HIF1α-Induced Glycolysis Metabolism Is Essential to the Activation of Inflammatory Macrophages by Ting Wang, Huiying Liu, Guan Lian, Song-Yang Zhang, Xian Wang, Changtao Jiang

“…Further results revealed that macrophage M1 polarization, induced by HIF1α overexpression, was via upregulating the mRNA expression of the genes related to the glycolysis metabolism. …”
Get full text

Clostridium butyricum attenuates LPS-induced myocardial injury in septic mice by modulating CD4 + CD25 + FOXP3 + Treg by Jinglin Zhao, Liuli Wu, Rupan Zhang, Mei Yuan, Junchao Huang, Xiongfei Jia, Xiaoqin Mao

“…Intestinal microbiota imbalance plays a crucial role in sepsis regulation. Using 16srRNA technology, we explored how intestinal colonization of Clostridium butyricum over 28 days impacted mice with LPS-induced sepsis. …”
Get full text

Gastrointestinal protozoa in pet cats from Anhui province: prevalence and molecular characterization by Hao Zhang, Xing Tong, Zhonghui Ma, Tao Zhang, Feng Wu, Qiong Wu, Congshan Yang, Chunyang Han

“…The evolutionary relationships and genetic characteristics of G. intestinalis based on the GDH gene, Cryptosporidium based on the SSU rRNA gene, and E. bieneusi based on the ITS sequence were assessed: five cases of G. intestinalis were identified, with four belonging to assemblage F and one to zoonotic assemblage B, two Cryptosporidium cases were identified as Cryptosporidium felis, and all eight E. bieneusi cases were identified as belonging to group 1, with three cases being genotype D, three EbpA, and two EbpC.DiscussionAge, neutering status, and deworming were identified as potential risk factors. …”
Get full text

Metataxonomic Characterization of Enriched Consortia Derived from Oil Spill-Contaminated Sites in Guimaras, Philippines, Reveals Major Role of Klebsiella sp. in Hydrocarbon Degrada... by Kiara Nicole D. Rodriguez, Russel T. Santos, Michael Joseph M. Nagpala, Rina B. Opulencia

“…The consortia that obtained the highest percent degradation for each of the four hydrocarbons were “B2” (92.34% diesel degraded), “A5” (85.55% hexadecane degraded), “B1” (74.33% hexane degraded), and “B7” (63.38% xylene degraded). Illumina MiSeq 16S rRNA gene amplicon sequencing revealed that the dominant phyla in all consortia are Pseudomonadota (previously Proteobacteria), followed by Bacillota (previously Firmicutes). …”
Get full text

Virtual Screening for Potential Inhibitors of NS3 Protein of Zika Virus by Maheswata Sahoo, Lingaraja Jena, Sangeeta Daf, Satish Kumar

“…Zika virus (ZIKV) is a mosquito borne pathogen, belongs to Flaviviridae family having a positive-sense single-stranded RNA genome, currently known for causing large epidemics in Brazil. …”
Get full text

Mutation yellow in agouti loci prevents age-related increase of skeletal muscle genes regulating free fatty acids oxidation by Y. V. Piskunova, A. Y. Kazantceva, A. V. Baklanov, N. M. Bazhan

“…C57Bl/6J mice bearing a dominant autosomal mutation Ay (Ay /a mice) and mice of the standard genotype (a/a mice, control) have been studied in three age groups: 10, 15 and 30 weeks. The relative mRNA level of genes was measured by real-time PCR in skeletal muscles (uncoupling protein 3 (Ucp3) and carnitine palmitoyl transferase 1b (Cpt1b) (free fatty acids oxidation), solute carrier family 2 (facilitated glucose transporter), member 4 (Slc2a4) (glucose uptake)), in WAT lipoprotein lipase (Lpl) (triglyceride deposition), hormone-sensitive lipase (Lipe) (lipid mobilization), and Slc2a4 (glucose uptake)), and in BAT: uncoupling protein 1 (Ucp1) (energy expenditure). …”
Get full text

PD-L1 Expression and CD8+ T Cell Infiltration Predict a Favorable Prognosis in Advanced Gastric Cancer by Yangyang Wang, Chunchao Zhu, Wei Song, Jun Li, Gang Zhao, Hui Cao

“…PD-L1 expression was positively correlated with CD8+ T cell infiltration. Then, PD-L1 and CD8A mRNA expression was analyzed using gastric cancer data from the TCGA database, confirming a positive correlation. …”
Get full text

Downregulation of Hlx Closely Related to the Decreased Expressions of T-bet and Runx3 in Patients with Gastric Cancer May Be Associated with a Pathological Event Leading to the Im... by Yan Xu, Jingjing Gao, Zhaoliang Su, Xiaoli Dai, Yazhen Li, Yingzhao Liu, Jianguo Chen, Jia Tong, Yun Zhang, Chaoyang Wu, Dong Zheng, Shengjun Wang, Huaxi Xu

“…In addition, the Runx3 expression was also downregulated with the lower T-bet mRNA level. These results suggested that the decreased Hlx expression was closely associated with T-bet and Runx3 downregulations and may contribute to the development of gastric cancer.…”
Get full text

Electroacupuncture-Regulated miR-34a-3p/PDCD6 Axis Promotes Post-Spinal Cord Injury Recovery in Both In Vitro and In Vivo Settings by Lili Ma, Lizhong Ma, Yu Yang, Ting Chen, Limin Wang, Qilong Deng

“…In our study, based on bioinformatics prediction, we found that miR-34a-3p might be an upstream regulator miRNA for PDCD6, which was subsequently validated through combined utilization of the qRT-PCR, western blot, and dual-luciferase reporter system. …”
Get full text

YTHDF1-mediated m6A modification of GBP4 promotes M1 macrophage polarization in acute lung injury by Fengan Cao, Shilei Wang, Qiuyue Tan, Junna Hou, Yunlu Li, Wentao Ma, Shilong Zhao, Jing Gao

“…YTHDF1 was found to enhance GBP4 expression by recognizing m6A sites on its mRNA, which was linked to reduced inflammation in MLE-12 cells upon YTHDF1 knockdown. …”
Get full text

Cardiovascular Risk in Systemic Autoimmune Diseases: Epigenetic Mechanisms of Immune Regulatory Functions by Chary López-Pedrera, Carlos Pérez-Sánchez, Manuel Ramos-Casals, Monica Santos-Gonzalez, Antonio Rodriguez-Ariza, Ma José Cuadrado

“…Epigenetic regulatory mechanisms comprise DNA methylation, histone modifications, and microRNA activity, all of which act upon gene and protein expression levels. …”
Get full text

Molecular reassessment of diaporthalean fungi associated with strawberry, including the leaf blight fungus, Paraphomopsis obscurans gen. et comb. nov. (Melanconiellaceae) by Dhanushka Udayanga, Shaneya D. Miriyagalla, Dimuthu S. Manamgoda, Kim S. Lewers, Alain Gardiennet, Lisa A. Castlebury

“…Combined analyses of four nuclear loci, 28S ribosomal DNA/large subunit rDNA (LSU), ribosomal internal transcribed spacers 1 and 2 with 5.8S ribosomal DNA (ITS), partial sequences of second largest subunit of RNA polymerase II (RPB2) and translation elongation factor 1-α (TEF1), were used to reconstruct a phylogeny for these pathogens. …”
Get full text

Phillyrin ameliorates DSS-induced colitis in mice via modulating the gut microbiota and inhibiting the NF-κB/MLCK pathway by Tong Li, Guiqiu Hu, Shoupeng Fu, Di Qin, Zheyu Song

“…This mechanism results in the preservation of the integrity of the intestinal barrier. The outcomes of 16S rRNA sequencing suggest that PHY (50 mg/kg) augmented the relative abundance of certain probiotic strains, including Lactobacillaceae and Lachnospiraceae. …”
Get full text

Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate Gyrus by Jonathan Soulé, Zsuzsa Penke, Tambudzai Kanhema, Maria Nordheim Alme, Serge Laroche, Clive R. Bramham

“…RT-PCR analysis from dentate gyrus tissue collected shortly after training did not reveal learning-specific changes in Arc mRNA expression. In situ hybridization and immunohistochemistry were therefore used to assess possible sparse effects on gene expression. …”
Get full text

TEC-mediated tRF-31R9J regulates histone lactylation and acetylation by HDAC1 to suppress hepatocyte ferroptosis and improve non-alcoholic steatohepatitis by Juanjuan Zhu, Xian Wu, Mao Mu, Quan Zhang, Xueke Zhao

“…Abstract Background Tectorigenin (TEC) is a monomer of anthocyanin, which we found exhibits hepatoprotective effects. tRNA-derived fragments (tRFs) and ferroptosis play important roles in the pathogenesis of non-alcoholic steatohepatitis (NASH). …”
Get full text

Acid sphingomyelinase modulates anxiety-like behavior likely through toll-like receptor signaling pathway by Huiqi Yuan, Yanan Xu, Hailun Jiang, Meizhu Jiang, Luofei Zhang, Shifeng Wei, Cao Li, Zhigang Zhao

“…In the present study, we employed Asm-knockout (Asm KO) mice to investigate the association between Asm and anxiety using behavioral tests, RNA sequencing, q-PCR, immunohistochemical staining, and other methods. …”
Get full text

Exosomal miR-141-3p from PDLSCs Alleviates High Glucose-Induced Senescence of PDLSCs by Activating the KEAP1-NRF2 Signaling Pathway by Min Liu, Rui Chen, Yunxuan Xu, Jiawen Zheng, Min Wang, Ping Wang

“…Further analyses revealed that microRNA-141-3p (miR-141-3p) was expressed at relatively high levels in PDLSC-Exos and was instrumental in PDLSC-Exo-mediated restoration by downregulating Kelch-like ECH-associated protein 1 (KEAP1), which is a negative regulator of NRF2 expression. …”
Get full text

Fungal planktonic community related to salinity and temperature in an oligotrophic sea by Ashwag A. Asseri, Alexandra Coello-Camba, Susana Agustí

“…We collected water samples from the photic layer at six sites along the Red Sea basin and analyzed the fungal community by targeting the 28S rRNA gene. Our results showed that Chytridiomycota dominated these communities, accounting for 85% of reads, followed by members of the divisions Basidiomycota (4.7%) and Cryptomycota (4.13%). …”
Get full text

Role of silent mutations in KRAS-mutant tumors by Jun Lu, Chao Zhou, Feng Pan, Hongyu Liu, Haohua Jiang, Hua Zhong, Baohui Han, Xiangxiang Pan

“…Silent mutations, which do not alter protein sequences but can affect RNA stability and translational efficiency, pose intriguing questions regarding their contribution to tumor biology. …”
Get full text

Resveratrol, a food-derived polyphenol, promotes Melanosomal degradation in skin fibroblasts through coordinated activation of autophagy, lysosomal, and antioxidant pathways by Saki Okamoto, Saya Kakimaru, Mayuko Koreishi, Mika Sakamoto, Yoshimasa Nakamura, Hideya Ando, Yoshio Tsujino, Ayano Satoh

“…At concentrations of 25–50 μM, resveratrol increased autophagy as measured by microtubule-associated protein 1A/1B-light chain 3 (LC3)-II/LC3-I ratio and enhanced lysosomal activity as assessed by a lysosomal activity reporter system. RNA sequencing revealed upregulation of lysosomal and autophagy-related genes, including cathepsins. …”
Get full text