Decoding the aroma of Rosa canina L.: Chemical composition and gene expression. by Parisa Jariani, Ali-Akbar Shahnejat-Bushehri, Roohangiz Naderi, Meisam Zargar, Mohammad Reza Naghavi

“…<h4>Methods</h4>Essential oils (EOs) were extracted from petals at five developmental stages (S1-S5) and analyzed using Gas Chromatography-Mass Spectrometry (GC-MS), identifying 20 distinct compounds. RNA isolation and quantitative real-time PCR (qRT-PCR) analysis were performed to assess gene expression.…”
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Inhibition of GSK3β is synthetic lethal with FHIT loss in lung cancer by blocking homologous recombination repair by Shishi Tao, Yue Pu, Eun Ju Yang, Guowen Ren, Changxiang Shi, Li-Jie Chen, Liang Chen, Joong Sup Shim

“…Pharmacological inhibition or siRNA depletion of GSK3β selectively suppressed the growth of FHIT-deficient lung cancer tumors in vitro and in animal models. …”
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Identifikasi Ekspresi Gen Myc pada Subkultur MCF-7 Breast Cancer Cell Line dengan Sel Punca by Yuastika Puspita Sari, Hirowati Ali, Aswiyanti Asri

“…Kemudian, dilakukan isolasi RNA, sintesis cDNA, dan ekspresi Myc diperiksa menggunakan PCR dan elektroforesis. …”
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A Reduction in Selenoprotein S Amplifies the Inflammatory Profile of Fast-Twitch Skeletal Muscle in the mdx Dystrophic Mouse by Craig Robert Wright, Giselle Larissa Allsopp, Alex Bernard Addinsall, Natasha Lee McRae, Sofianos Andrikopoulos, Nicole Stupka

“…In the extensor digitorum longus (EDL) muscles, mRNA expression of monocyte chemoattractant protein 1 (Mcp-1) (P=0.034), macrophage marker F4/80 (P=0.030), and transforming growth factor-β1 (Tgf-β1) (P=0.056) were increased in mdx:Seps1−/+ mice. …”
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An annotated high-content fluorescence microscopy dataset with EGFP-Galectin-3-stained cells and manually labelled outlineszenodo by Salma Kazemi Rashed, Malou Arvidsson, Rafsan Ahmed, Sonja Aits

“…Images were acquired on a Thermo Fischer CX7 high-content imaging system at 20x magnification created as part of an RNA interference screen with a modified U2OS osteosarcoma cell line. …”
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LongReadSum: A fast and flexible quality control and signal summarization tool for long-read sequencing data by Jonathan Elliot Perdomo, Mian Umair Ahsan, Qian Liu, Li Fang, Kai Wang

“…We also demonstrate a few examples using LongReadSum to analyze cDNA sequencing, direct RNA sequencing, ONT reduced representation methylation sequencing (RRMS), and whole genome sequencing (WGS) data.…”
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SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice by Chunxia Yu, Peng Zhang, Sujuan Liu, Yanmei Niu, Li Fu

“…Fecal microbiota composition and function were assessed by 16S rRNA sequencing. The sequencing results showed that SESN2−/− mice displayed differed microbiome profile from WT mice. …”
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Plant Growth Promotion and Biocontrol Potentiality of Endophytes Isolated from Root Nodules of Sulla flexuosa L. Plants by Samia Hamane, Anass El Yemlahi, Mounir Hassani Zerrouk, Ouiam El Galiou, Amin Laglaoui, Mohammed Bakkali, Abdelhay Arakrak

“…In vitro experiments revealed the strains’ potential to withstand salt and drought stresses by being able to grow in high concentrations of NaCl and PEG. Based on 16S rRNA gene sequencing, the strains were identified as Enterobacter and Serratia. …”
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Knockdown of zebrafish tmem242 enhances the production of ROS that signals to increase f9a expression resulting in DIC-like condition by Afnan Deebani, Jabila Mary, Sanchi Dhinoja, Ayah Al Qaryoute, Weam Fallatah, Pudur Jagadeeswaran

“…While thrombocyte production and aggregation were unaffected, coagulation was impaired, with delayed fibrin and thrombus formation. Notably, mRNA levels of several clotting factor genes, including coagulation factor 5 (f5), coagulation factor 7 (f7), and coagulation factor 9a (f9a), were elevated, except for coagulation factor 8 (f8). …”
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The Alteration and Clinical Significance of Th22/Th17/Th1 Cells in Patients with Chronic Myeloid Leukemia by Ping Chen, Min Wang, Daqi Li, Yan Jia, Na He, Wei Li, Daoxin Ma, Chunyan Ji

“…Plasma IL-22, IL-17, and IFN-r concentrations were measured by ELISA. AHR and RORC mRNA expressions were examined by RT-PCR. The frequencies of Th22, Th17, and Th1 cells, along with the expression of specific transcription factors RORC and AHR, were significantly decreased in ND patients compared with healthy controls, while all these abnormality recovered in CP patients. …”
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Blocking the Hepatic Branch of the Vagus Aggravates Hepatic Ischemia-Reperfusion Injury via Inhibiting the Expression of IL-22 in the Liver by Heng Zhou, Juling Xu, Sanxiong Huang, Ying He, Xiaowei He, Lu Guo, Shi Yin, Sheng Lu

“…Further experiments confirmed that Hv significantly downregulated the expression of IL-22 protein and mRNA in the liver, blocking the activation of the STAT3 pathway. …”
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Association of H-Type Hypertension with miR-21, miR-29, and miR-199 in Kazahks of Xinjiang, China by Xin He, RuLin Ma, Yu Li, Haixia Wang, YiZhong Yan, YiDan Mao, ShengYu Liao, XueYing Sun, ShuXia Guo, Heng Guo

“…Then, we analyzed the effect of MTHFR 677C > T polymorphism on the association between the above miRNA and H-type hypertension. Method. In this study, the expression of miR-21, miR-29, and miR-199 was quantitatively measured in 120 serum samples and then stratified according to the C677T polymorphism to analyze the relationship between target miRNAs and HHcy. …”
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Recent updates of centromere proteins in hepatocellular carcinoma: a review by Zhongyuan Yang, Wenjiao Chen, Yunhui Liu, Yuxin Niu

“…For example, small ribonucleic acid (RNA) can silence CENP expression and prevent the occurrence and development of liver cancer. …”
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Machine Learning Enabled Prediction of Biologically Relevant Gene Expression Using CT‐Based Radiomic Features in Non‐Small Cell Lung Cancer by Shrey S. Sukhadia, Christoph Sadée, Olivier Gevaert, Shivashankar H. Nagaraj

“…Methods In a retrospective study of two NSCLC patient cohorts separated by 5 years, we performed a radiogenomic analysis of previously disseminated data from 2018 (n = 116) and newly acquired data from 2023 (n = 44) using RNA sequencing and lung CT images. Combining the data from two cohorts post binarization (of gene expression) or batch normalization (of radiomic features) in each cohort proved to be a better approach as compared to training the model on one cohort and validating on the other. …”
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Inulin Reduces Kidney Damage in Type 2 Diabetic Mice by Decreasing Inflammation and Serum Metabolomics by Jiayuan He, Xiang Li, Man Yan, Xinsheng Chen, Chang Sun, Jiajun Tan, Yinsheng Song, Hong Xu, Liang Wu, Zhengnan Yang

“…The administration of inulin not only ameliorated renal injury but also resulted in a reduction in the mRNA expressions of inflammatory factors in the spleen and serum oxidative stress levels, when compared to the DM group. …”
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Heme Oxygenase 1/Peroxisome Proliferator-Activated Receptor Gamma Pathway Protects Intimal Hyperplasia and Mitigates Arteriovenous Fistula Dysfunction by Regulating Oxidative Stres... by Tingfei Xie, Yunpeng Xu, Lecai Ji, Xiaolu Sui, Aisha Zhang, Yanzi Zhang, Jihong Chen

“…AVF model mice were established, and the vascular tissues from the arteriovenous anastomosis site were sent for mRNA sequencing. Differentially expressed mRNAs (DEmRNAs) were screened and subjected to functional analysis. …”
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Exosomes derived from liver failure patients’ plasma stimulated mesenchymal stem cells alleviate acute liver failure by Zhuoran Wang, Jun Ling, Shaoli You, Bing Zhu

“…Methods Untreated exosomes (NC-Exos) and LF-Exos were extracted and characterized by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), western blotting, and miRNA sequencing. We then examined the protective effects of LF-Exos on hepatocytes acutely injured by D-galactosamine (D-GalN)/lipopolysaccharide (LPS) co-treatment and on a mouse model of acute liver failure (ALF). …”
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Tenidap sodium inhibits secretory non-pancreatic phospholipase A2 synthesis by foetal rat calvarial osteoblasts by W. Pruzanski, B. P. Kennedy, H. van den Bosch, E. Stefanski, M. Wloch, P. Vadas

“…Northern blot analysis showed that TD did not affect the sPLA2 mRNA levels; however, immunoblotting showed a dose-dependent reduction in sPLA2 enzyme. …”
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GENDER-SPECIFIC INFLUENCE OF Aу MUTATION ON PROGENY METABOLIC PHENOTYPE, FETAL GROWTH AND PLACENTAL GENE EXPRESSION IN MICE by E. N. Makarova, E. I. Denisova, V. V. Kozhevnikova, A. E. Kuleshova

“…On day 13 of pregnancy, the mRNA levels of the examined genes did not differ in placentas of male and female fetuses in ɑ/ɑ  females. …”
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The Prognostic Model Established by the Differential Expression Genes Based on CD8+ T Cells to Evaluate the Prognosis and the Response to Immunotherapy in Osteosarcoma by Yu Chen, Wei Yan, Hongqing Wang, Zhiliang Ou, Huihong Chen, Zhenhua Huang, Jinlian Yang, Biqiong Liu, Fanjie Ou, Huang Zhang

“…In this study, we obtained the single-cell RNA sequencing datasets of OS patients from public databases and defined 34 cell clusters by dimensional reduction and clustering analysis. …”
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