SPOCK2 controls the proliferation and function of immature pancreatic β-cells through MMP2 by Katarzyna Blaszczyk, Anna P. Jedrzejak, Natalia Ziojla, Ekaterina Shcheglova, Karolina Szarafin, Artur Jankowski, Christine A. Beamish, Jolanta Chmielowiec, Omaima M. Sabek, Ashok Balasubramanyam, Sanjeet Patel, Malgorzata Borowiak

“…Through bidirectional expression modulation and single-cell RNA-seq, we identified SPOCK2, an ECM protein, as an inhibitor of immature β-cell proliferation. …”
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Identified VCAM1 as prognostic gene in gastric cancer by co-expression network analysis by Wenjuan Li, Hong Gao, Jianjun Liu

“…This study aims to identify genetic variances and potential diagnostic and therapeutic approaches for diverse types of gastric cancer utilizing an extensive dataset. Data from RNA sequencing and clinical pathological details were acquired from The Cancer Genome Atlas (TCGA) database and the Gene Expression Omnibus (GEO) dataset. …”
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BuDDI: Bulk Deconvolution with Domain Invariance to predict cell-type-specific perturbations from bulk. by Natalie R Davidson, Fan Zhang, Casey S Greene

“…BuDDI utilizes domain adaptation techniques to effectively integrate available corpora of case-control bulk and reference scRNA-seq observations to infer cell-type-specific perturbation effects. …”
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Hemorrhagic Shock Induces a Rapid Transcriptomic Shift of the Immune Balance in Leukocytes after Experimental Multiple Injury by Lisa Debler, Annette Palmer, Sonja Braumüller, Bettina Klohs, Tom Eirik Mollnes, Karlheinz Holzmann, Markus Huber-Lang, Rebecca Halbgebauer

“…Four hours after trauma, corresponding to three hours after induction of hemorrhage, RNA was isolated from all peripheral blood leukocytes, and a microarray analysis was performed. …”
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DNA Methylation May be Involved in the Analgesic Effect of Hyperbaric Oxygen via Regulating FUNDC1 by Kun Liu, Hao Wu, Rui Gao, Guang Han

“…HBO downregulated DNMT3a mRNA and protein expression, but not those of DNMT1 and DNMT3b. …”
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LIMD1-AS1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down-regulating miR-29c-3p by Yongsheng Yu, Nan He, Zhaolu Song, Chang Wang, Jinhuang Xu

“…Functionally, LIMD1-AS1 modulated the expression of miR-29c-3p to potentiate the proliferative, migratory, and invasive capabilities of PCa cells while concurrently inhibiting apoptosis. Conclusion LncRNA LIMD1-AS1 promotes the advancement of PCa by regulating miR-29c-3p, indicating that LIMD1-AS1/miR-29c-3p axis could serve as potential therapeutic targets for the therapeutic intervention of PCa.…”
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Differentiation value of miR-26b for major depressive disorder, schizophrenia, generalized anxiety disorder by Lingming Kong, Xiaoli Zhu, Liyi Zhang, Shengdong Chen

“…Materials and Methods: This study aimed to investigate the differentiation value of peripheral microRNA-26b expression in 52 patients of MDD, SZ, and GAD, respectively, and 52 controls. …”
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Differentiation of <i>Escherichia coli</i> and <i>Shigella flexneri</i> by Metabolite Profiles Obtained Using Gold Nanoparticles-Based Surface-Assisted Laser Desorption/Ionization... by Adrian Arendowski

“…<i>Escherichia coli</i> and <i>Shigella flexneri</i> are challenging to differentiate using methods such as phenotyping, 16S rRNA sequencing, or protein profiling through matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) due to their close relatedness. …”
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CIRBP Regulates Pancreatic Cancer Cell Ferroptosis and Growth by Directly Binding to p53 by Hongqiang Gao, Ran Xie, Rong Huang, Chonglin Wang, Yue Wang, Dongdong Wang, Kaimin Liu, Conghui Yang, Qingxiong Yang, Long Chen

“…RIP results showed that CIRBP antibody significantly enriched p53 RNA, indicating that it could directly bind to p53. …”
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Pyrogallol acts as a novel anticancer factor to enhance the sensitivity to cisplatin in ovarian cancer cells through inducing miR-15a upregulation by Azin Shirani, Razieh Mirabbasi, Leila Shabani, Somayeh Reiisi

“…Also, the qPCR results showed a significant increase in the expression of miR-15a and a decrease in the expression of miRNA target genes under treatment with the drugs both separately and in their combination. …”
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Evaluation of Plaque Stability of Advanced Atherosclerotic Lesions in Apo E-Deficient Mice after Treatment with the Oral Factor Xa Inhibitor Rivaroxaban by Qianxing Zhou, Florian Bea, Michael Preusch, Hongjie Wang, Berend Isermann, Khurrum Shahzad, Hugo A. Katus, Erwin Blessing

“…Real time-PCR from thoracic aortas revealed that rivaroxaban (5 mg/kg/day) treatment reduced mRNA expression of inflammatory mediators, such of IL-6, TNF-α, MCP-1, and Egr-1 (P<.05). …”
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Th1/17-Biased Inflammatory Environment Associated with COPD Alters the Response of Airway Epithelial Cells to Viral and Bacterial Stimuli by Yifan Chen, Rakesh K. Kumar, Paul S. Thomas, Cristan Herbert

“…Primary AECs pretreated with Th1/17 cytokines exhibited enhanced expression of mRNA for proinflammatory cytokines in response to poly I:C. …”
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Anti-Inflammatory Activity of Ferula assafoetida Oleo-Gum-Resin (Asafoetida) against TNF-α-Stimulated Human Umbilical Vein Endothelial Cells (HUVECs) by Leila Mobasheri, Mohsen Khorashadizadeh, Hossein Safarpour, Maryam Mohammadi, Gholamreza Anani Sarab, Vahid Reza Askari

“…In addition, EEA pretreatment decreased TNF-α-induced mRNA expression of VCAM-1 and surface protein expression of ICAM-1 in the target cells. …”
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Whole genome sequencing completes the molecular genetic testing workflow of patients with Lynch syndrome by Klaudia Horti-Oravecz, Anikó Bozsik, Tímea Pócza, Ildikó Vereczkey, Tamás Strausz, Erika Tóth, Tatiana Sedlackova, Diana Rusnakova, Tomas Szemes, István Likó, Edit Oláh, Henriett Butz, Attila Patócs, János Papp, Vince Kornél Grolmusz

“…Here we present a DNA-, RNA- and tumor tissue-based WGS prioritization workflow for patients with a suspicion of LS where MGPT detected no LS-related PV. …”
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Pediatric kidney transplant recipients are at an increased risk for dysbiosis by Gizem Yılmaz, Seha Saygılı, Ayşe Ağbaş, Esra Karabağ Yılmaz, Ahmet Variş, Nur Canpolat

“…The gut microbiota was assessed using 16S rRNA gene sequencing, with alpha and beta diversity, as well as all statistical analyses, conducted using the Phyloseq library in the R programming language. …”
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Descriptores espacio-frecuencia para identificación automática de patrones de textura en productos textiles utilizando aprendizaje supervisado by Arley Bejarano Martínez, Andres Felipe Calvo Salcedo, Carlos Alberto Henao Baena

“…Para la etapa de clasificación se analiza el uso de tres métodos del estado del arte como lo son las Máquinas de Soporte Vectorial (SVM), las Redes Neuronales Artificiales (RNA) y el Proceso Gaussiano (GP). Para validar el método se construye una base de datos anotada con diez tipos de telas con un total de 1000 fotos, a las cuales se le aplica el proceso caracterización y clasificación por medio de un experimento Montecarlo. …”
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MAIP1-Related Tumor Immune Infiltration: As a Potential Prognostic Biomarker for Esophageal Cancer by Xiaoyu He, Fu Gan, Yongjian Lin, Guoqing Liu, Yunhua Lin, Dongxu Chen

“…This study aims to determine the clinical significance of MAIP1 in EC and explores its potential molecular mechanisms regulating tumor immune infiltration. Methods. We obtained RNA-seq datasets and corresponding clinical data for EC patients from the Cancer Genome Atlas (TCGA) database via the UCSC Xena browser to extract MAIP1 expression and plot survival curves to determine their prognosis. …”
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Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections by Sandra Menting, Khoa T. D. Thai, Tran T. T. Nga, Hoang L. Phuong, Paul Klatser, Katja C. Wolthers, Tran Q. Binh, Peter J. de Vries, Marcel Beld

“…An internally controlled, generic real-time PCR was developed and validated by testing serial dilutions of a DENV positive control RNA in the presence of a fixed amount of IC with results showing a good linearity (R2=0.9967) and a LOD of at least 1.95×104 copies/mL. …”
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UCH-L1 Inhibitor Alleviates Nerve Damage Caused by Moyamoya Disease by Minghua Xu, Xiaomin Zhao, Jiang Zhao, Zhisheng Tan, Chengshi Zhang, Yun Huang, Huiping Zhong, Meifeng Guo, Chen Zhang, Ping Ye, Wentao Zheng

“…Exosomes were isolated from the serum of patients with MMD and healthy controls. The total RNA was extracted from the exosomes, and the level of GFAP and UCH-L1 between the serum exosomes of the two groups was analyzed by a quantitative reverse transcription-polymerase chain reaction and western blot. …”
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Effects of Ginkgo biloba Extract on Inflammatory Mediators (SOD, MDA, TNF-α, NF-κBp65, IL-6) in TNBS-Induced Colitis in Rats by Yan-Hong Zhou, Jie-Ping Yu, Yi-Fei Liu, Xiao-Jun Teng, Mei Ming, Peng Lv, Ping An, Shi-Quan Liu, Hong-Gang Yu

“…The results showed that EGB could significantly ameliorate macroscopic and histological damage, evidently elevate the activities of SOD and reduce the contents of MDA, inhibit the protein and mRNA expressions of TNF-α, NF-κBp65, and IL-6 in the colon tissues of experimental colitis in a dose-dependent manner compared with the model group. …”
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