Canagliflozin attenuates kidney injury, gut-derived toxins, and gut microbiota imbalance in high-salt diet-fed Dahl salt-sensitive rats by Lili He, Qingjuan Zuo, Sai Ma, Guorui Zhang, Zhongli Wang, Tingting Zhang, Jianlong Zhai, Yifang Guo

“…This study employed 16S rRNA sequencing technology and liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based metabolic profiling combined with advanced differential and association analyses to investigate the correlation between the microbiome and the metabolome in male DSS rats.Results A high-salt diet disrupted the balance of the intestinal flora and increased toxic metabolites (methyhistidines, creatinine, homocitrulline, and indoxyl sulfate), resulting in severe kidney damage. …”
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Chloride intracellular channel CLIC3 mediates fibroblast cellular senescence by interacting with ERK7 by Changjiao Luan, Yue Gao, Jun Zhao, Xiaohui Zhang, Chaofan Wang, Wentao Sun, Yucheng Li, Xinxing Yang, Jiaxiao Chen, Weili Liu, Weijuan Gong, Xingjie Ma

“…In this study, we conducted RNA sequencing on bleomycin-induced senescent lung tissues from mice and identified Clic3 as the most significantly upregulated CLIC member. …”
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Pre-therapeutic efficacy of the CDK inhibitor dinaciclib in medulloblastoma cells by Marta Buzzetti, Sonia Morlando, Dimitrios Solomos, Ammara Mehmood, Alexander W. I. Cox, Mattia Chiesa, Yuri D’Alessandra, Michela Garofalo, Caroline H. Topham, Gianpiero Di Leva

“…We found that dinaciclib-triggered apoptosis is triggered by CDK9 inhibition and the resultant reduction in RNA pol II phosphorylation, which leads to the downregulation of the oncogenic marker MYC, and the anti-apoptotic protein MCL-1. …”
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Molecular Detection of Ehrlichia ruminantium in Cattle From Different Agro‐Ecological Zones of Cameroon: Implication for the Understanding of the Heartwater Epidemiology by Esthelline Yangea Tchounkeu, Barberine Assongo Silatsa, Rolin Mitterran Ndefo Kamga, Pythagore Soubgwi Fogue, Gustave Simo

“…DNA was extracted from blood and semi‐nested PCR targeting the 16S rRNA gene of E. ruminantium was used to search for this bacterium. …”
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Combined PARP14 inhibition and PD-1 blockade promotes cytotoxic T cell quiescence and modulates macrophage polarization in relapsed melanoma by Chun Wai Wong, Rotem Leshem, Kieran Neil Sefton, I-Hsuan Lin, Dervla Tamara Isaac, Mario Niepel, Adam Hurlstone

“…Using preclinical models and single-cell RNA sequencing, we investigated immune and tumor cell adaptations to PARP14 inhibition combined with PD-1 blockade.Results Combining PARP14 inhibition and PD-1 blockade suppressed tumor-associated macrophages while increasing proinflammatory memory macrophages. …”
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Exploring genetic structures and shared sites between alcohol, cheese intake, and inflammatory bowel disease by Zhifang Huang, Weichao Yuan

“…Furthermore, both conjFDR and MTAG analyses identified multiple shared genetic loci, with nine genes (Y_RNA, DENND1B, GCKR, KPNA7, CLN3, SLC39A8, FUT2, ERAP2, and SMAD3) being.ConclusionThe present study provides genetic evidence supporting the comorbidity of alcohol and cheese intake with IBD, offering novel insights into potential strategies for the prevention and treatment of IBD through the modulation of alcohol and cheese consumption.…”
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Characterization of infectious salmon anaemia virus survival and infectivity in seawater by Simon Chioma Weli, Marit Måsøy Amundsen, Ole Bendik Dale, Sonal Jayesh Patel

“…In addition, the intensity of positive IFAT staining of cells infected with the eluted spiked seawater was proportional to the viral load obtained by RT-qPCR. However, while viral RNA could be detected at high amounts in samples from day five and day eight respectively for ISAV-G and ISAV-H incubated at 15 ℃, we could not demonstrate infective particles from these samples. …”
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Microbiome analysis in individuals with human papillomavirus oral infection by David Israel Escobar Marcillo, Grete Francesca Privitera, Francesca Rollo, Alessandra Latini, Eugenia Giuliani, Maria Benevolo, Massimo Giuliani, Barbara Pichi, Raul Pellini, Maria Gabriella Donà

“…DNA purified from oral rinse-and-gargles of HIV-infected (HIV+) and HIV-uninfected (HIV−) individuals were used for 16S rRNA gene V3–V4 region amplification and sequencing. …”
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Impact of sex on the adaptation of adult mice to long consumption of sweet-fat diet by N. M. Bazhan, T. V. Iakovleva, A. D. Dubinina, E. N. Makarova

“…Weights of body, liver and fat depots, blood concentrations of hormones and metabolites, liver fat, and mRNA levels of genes involved in regulation of energy metabolism in the liver, perigonadal and subcutaneous white adipose tissue (pgWAT, scWAT) and brown adipose tissue (BAT) were measured. …”
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Polydatin ameliorates early brain injury after subarachnoid hemorrhage through up-regulating SIRT1 to suppress endoplasmic reticulum stress by Yuwei Han, Guangzhi Hao, Song Han, Tingzhun Zhu, Yushu Dong, Ligang Chen, Xinyu Yang, Xiaoming Li, Hai Jin, Guobiao Liang

“…Western blot and quantitative real-time polymerase chain reaction (PCR) analyses were performed to examine protein and mRNA expressions related to ER stress. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was used to detect cell apoptosis, and transmission electron microscopy was used to observe the ultrastructure of the endoplasmic reticulum.ResultsThe results indicated that PD significantly reduced brain edema and Evans blue extravasation after SAH, improving neurological function. …”
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Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway by Hong Wei, Rui Bu, Qinghui Yang, Jing Jia, Tao Li, Qiuping Wang, Yanjun Chen

“…Exendin-4 treatment decreased the protein level of TXNIP. Moreover, RNA silencing of TXNIP mimicked the antipyroptotic actions of Exendin-4. …”
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The importance of fungi and of mycology for a global development of the bioeconomy by L. Lange, L. Bech, P.K. Busk, M.N. Grell, Y. Huang, M. Lange, T. Linde, B. Pilgaard, D. Roth, X. Tong

“…This knowledge can be obtained by studying the fungal proteome and metabolome; the biology of fungal RNA and epigenetics; protein expression, homologous as well as heterologous; fungal host/substrate relations; physiology, especially of extremophiles; and, not the least, the extent of global fungal biodiversity. …”
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Aqp 9 and Brain Tumour Stem Cells by Guri Fossdal, Einar O. Vik-Mo, Cecilie Sandberg, Mercy Varghese, Mari Kaarbø, Emily Telmo, Iver A. Langmoen, Wayne Murrell

“…However, immunostaining did not reveal increased protein expression of aqp9 in the tumourspheres containing progenitor cells; in contrast, its expression (both mRNA and protein) was high in differentiated cultures. …”
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Morphological, craniodental, and molecular characterization of Platyrrhinus guianensis (Chiroptera, Phyllostomidae) and second record for Brazil by Amanda C. da S. Lima, Samira B. Mendes, Ana P. M. Olímpio, Fábio H. S. Cardoso, Cleison L. da S. Costa, Elmary da C. Fraga, Maria C. Barros, Iracilda Sampaio

“…Molecular results provide the first public sequence of P. guianensis for the 16S rRNA gene and a new sequence for the COI gene, which will be useful in future studies for the species. …”
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Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain by Jia-Wen Leong, Syahril Abdullah, King-Hwa Ling, Pike-See Cheah

“…MicroRNAs (miRNAs) are small noncoding RNA known to regulate brain development. The expression of two novel miRNAs, namely, miR-344b and miR-344c, was characterized during mouse brain developmental stages in this study. …”
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Treatment of petrochemical wastewater with low C/N by IFFAS-ESMBR integrated composite process by ZHAO Xuan, LIU Tao, YANG Meng, QIAO Sen

“…Under the applied voltage of -1.2 V, the average growth rate of transmembrane pressure difference(TMP) of CNT-PVDF composite hollow fiber membrane was only 2.5 kPa/d, and the PN/PS of sludge on the membrane surface decreased gradually, indicating that the degree of irreversible membrane fouling was mild. 16S rRNA high-throughput sequencing showed that a large number of carbon and nitrogen removal functional bacteria, such as Rhodobacterales, Verrucomicrobiales, Anaerolineales and Xanthomonadales, were enriched in suspended sludge, membrane surface sludge and carrier surface sludge, which played a significant role in the degradation of organic matter and ammonia nitrogen in the reactor. …”
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Dysregulation of c-Jun (JUN) and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) in obese people and their predictive values for metabolic syndrome by Chenxi Yang, Yi Chen, Guangfeng Tang, Tongtong Shen, Li Li

“…Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to evaluate the messenger RNA levels of JUN and FOSB in the peripheral blood of healthy volunteers (lean and obese) and patients with MetS (lean and obese), along with that in the adipose tissue and peripheral blood of obese mouse model. …”
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Artificial simulated saliva, gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213 by Juanjuan Tian, Xiaomeng Wang, Xueliang Zhang, Xiaohong Chen, Mingsheng Dong, Xin Rui, Qiuqin Zhang, Mei Jiang, Wei Li

“…However, they can be hydrolyzed and utilized by gut microbiota, and short-chain fatty acids (SCFAs) level were increased after IPS1and IPS2 treatments. Furthermore, 16S rRNA sequencing analysis of fermentation samples were performed. …”
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Role of bovine respiratory syncytial virus in etiology of respiratory diseases on milk farms by S. V. Koteneva, A. V. Nefedchenko, T. I. Glotova, A. G. Glotov

“…Quantification of BRSV RNA demonstrated that maximum virus accumulation was observed in lungs and nasal charges and it confirms data on its tropism to pulmonary interstitium.…”
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Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer by Jong-Lyul Park, Mirang Kim, Kyu-Sang Song, Seon-Young Kim, Yong Sung Kim

“…We initially examined plasma miRNA expression levels in a screening cohort consisting of 15 patients with gastric cancer and 15 healthy controls from Korean population, using TaqMan quantitative real-time polymerase chain reaction. …”
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