Usp7 contributes to the tail regeneration of planarians via Islet/Wnt1 axis by Ang Liang, Jinglong Liu, Zhiyuan Zhang, Jing Xiao, Dezeng Liu, Zimei Dong, Guangwen Chen

“…Methods We identified an evolutionarily conserved gene, Usp7, and utilized RNA interference (RNAi), Quantitative real-time PCR (qRT-PCR), Whole-mount immunofluorescence, Tunnel, Whole-mount in situ hybridization (WISH), and western blotting to detect the function of Usp7 during the planarian regeneration. …”
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The clinical application of artificial intelligence in cancer precision treatment by Jinyu Wang, Ziyi Zeng, Zehua Li, Guangyue Liu, Shunhong Zhang, Chenchen Luo, Saidi Hu, Siran Wan, Linyong Zhao

“…In specific fields, including target prediction, targeted drug generation, immunotherapy response prediction, neoantigen prediction, and identification of long non-coding RNA, artificial intelligence offers promising perspectives. …”
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Evaluation of Cognitive Functions in People Living with HIV Before and After COVID-19 Infection by Dimtrios Basoulis, Elpida Mastrogianni, Irene Eliadi, Nikolaos Platakis, Dimitris Platis, Mina Psichogiou

“…A negative correlation was observed between MOCA scores and age (ρ = −0.283, <i>p</i> = 0.002), but not with a CD4 count at diagnosis (ρ = 0.169, <i>p</i> = 0.071) or initial HIV RNA load (ρ = 0.02, <i>p</i> = 0.984). In the subgroup with repeated testing, MOCA was correlated with the CD4 count (ρ = 0.238, <i>p</i> = 0.069 in the first and ρ = 0.319, <i>p</i> = 0.014 second test). …”
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Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine by Seyed Muhammad Hamid Malekpour, Moj Khaleghi, Alireza Akhtarpour

“…The results showed that the HM24 strain was the best tyrosinase-producing strain, and the most enzyme activity was in the peanut medium as well as in the soybean meal substrate. According to the 16S rRNA sequencing, the HM24 strain belonged to the genus Microbacterium with 99.97% identity. …”
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Increased RTN3 phenocopies nonalcoholic fatty liver disease by inhibiting the AMPK–IDH2 pathway by Hao Huang, Shuai Guo, Ya‐Qin Chen, Yu‐Xing Liu, Jie‐Yuan Jin, Yun Liang, Liang‐Liang Fan, Rong Xiang

“…The RTN3 transgenic mice exhibited the phenotypes of fatty liver and lipid accumulation. Single‐cell RNA sequencing analysis indicated that increased RTN3 might induce mitochondrial dysfunction. …”
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eEF-2K Deficiency Boosts the Virus-Specific Effector CD8⁺ T Cell Responses During Viral Infection by Liqing Wang, Benny Shone Song, Rayansh Poojary, Xiaofang Xiong, Xingcong Ren, Jin-Ming Yang, Jianxun Song

“…We found that eEF-2K-deficient (eEF-2K⁻/⁻) mice exhibited a significantly higher proportion of VACV-specific effector CD8⁺ T cells without compromising the development of VACV-specific memory CD8⁺ T cells. RNA sequencing demonstrated that eEF-2K⁻/⁻ VACV-specific effector CD8⁺ T cells had enhanced functionality, which improves their capacity to combat viral infection during the effector phase. …”
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Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats by Lukáš Alán, Tomáš Olejár, Monika Cahová, Jaroslav Zelenka, Zuzana Berková, Magdalena Smětáková, František Saudek, Radoslav Matěj, Petr Ježek

“…Aiming to compare delta cell physiology/pathology of GK to Wistar rats, we found that delta cell number increased over time as did somatostatin mRNA and delta cells distribution in PI is different in GK rats. …”
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Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal by Anicet Georges Bruno Manga, Godar Sene, André Amakobo Diatta, Tahir Abdoulaye Diop, Gérard Barroso, Diederik van Tuinen

“…After DNA extraction, nested PCR, and sequencing of the large subunit region of the rRNA gene, different phylotypes from rhizospheric soils, roots, and spores were compared by phylogeny in order to investigate the role of salinity in arbuscular fungal diversity. …”
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Gene Expression Analysis of Deduced Protein Sequence from the FLOWERING LOCUS C (FLC) Homolog during Vegetative and Reproductive Phases in Lepidium sativum L. by Parissa Jonoubi, Farkhondeh Rezanejad, Fatemeh Mahyapoor

“…Results showed that LsFLC expression levels were very high in the root and relatively high in leaves and stems, whereas no expression was allowed in flowers. Indeed, the mRNA levels were much higher during the vegetative phase compared to the transition to flowering, therefore agreeing with the known role of FLC as a promoter of vegetative growth and an inhibitor of flowering. …”
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Blockade of Vascular Endothelial Growth Factor Receptor 1 Prevents Inflammation and Vascular Leakage in Diabetic Retinopathy by Jianbo He, Hong Wang, Ying Liu, Wen Li, Dorothy Kim, Hu Huang

“…Protein expression and localization were examined by western blots (WB) and immunofluorescence (IF). mRNA expression was quantified by PCR array and real-time PCR. …”
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Possible Roles of Proinflammatory and Chemoattractive Cytokines Produced by Human Fetal Membrane Cells in the Pathology of Adverse Pregnancy Outcomes Associated with Influenza Viru... by Noboru Uchide, Kunio Ohyama, Toshio Bessho, Makoto Takeichi, Hiroo Toyoda

“…Influenza virus infection induced the mRNA expression of not only the proinflammatory cytokines but also chemoattractive cytokines, such as monocyte chemoattractant protein (MCP)-1, regulated on activation, normal T-cell expressed and secreted (RANTES), macrophage inflammatory protein (MIP)-1β, IL-8, growth-regulated oncogene (GRO)-α, GRO-β, epithelial cell-derived neutrophil-activating protein (ENA)-78, and interferon inducible protein (IP)-10 in cultured chorion cells. …”
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Azurin a potent anticancer and antimicrobial agent isolated from a novel Pseudomonas aeruginosa strain by Nourhan A. Zaghloul, Mona K. Gouda, Yasser Elbahloul, Nancy M. El Halfawy

“…Among twenty Pseudomonas aeruginosa isolates, we identified one isolate that demonstrated potent and remarkable azurin synthesis using the VITEK 2 system and 16S rRNA sequencing. The presence of the azurin gene was confirmed in the genomic DNA using specific oligonucleotide primers, and azurin expression was also detected in the synthesized cDNA, which revealed that the azurin expression is active. …”
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Identification of KIFC1 as an independent prognostic marker in renal clear cell carcinoma correlates with tumor proliferation and immune infiltration by Bin Du, Jia Wang, Jinping Zheng, Jing Huo, Pu Wang

“…We analyzed the prognostic value, associated signaling pathways, and regulatory mechanisms of KIFC1 in ccRCC through bioinformatics and proteomics. Concretely, both mRNA and protein expression levels of KIFC1 were dramatically upregulated. …”
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Diversity of microbial communities in cigar filler leaves with different initial water contents analyzed based on high-throughput sequencing technology by Yumeng Gao, Yumeng Gao, Yihui Wang, Bingqing Hou, Guo Zhang, Chun Jiang, Song Fang, Qian Wang, Yang Ning

“…IntroductionTo study the composition and succession of bacterial and fungal communities during the fermentation of cigar filler leaves with varying initial water contents, high-throughput sequencing technology was used to sequence the bacterial 16SrRNA genes and fungal ITS1 genes from cigar tobacco leaf samples. …”
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Enhancing single-cell transcriptomics using interposed anchor oligonucleotide sequences by Jianfeng Sun, Martin Philpott, Danson Loi, Gabriela Hoffman, Jonathan Robson, Neelam Mehta, Eleanor Calcutt, Vicki Gamble, Tom Brown, Tom Brown, Udo Oppermann, Adam P. Cribbs

“…Abstract Single-cell transcriptomics, which utilises barcodes and unique molecular identifiers (UMIs) for polyA+ mRNA capture, is compromised by oligonucleotide synthesis errors. …”
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Sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted mice by N. A. Feofanova, T. V. Yakovleva, E. N. Makarova, N. M. Bazhan

“…None of the genes studied (Ucp3, Cpt1 and Slc2a4) showed sex-related changes at mRNA levels in control groups, but females exposed to fasting demonstrated a significant increase in the expression of all genes as compared to control. …”
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Melanophilin-induced primary cilia promote pancreatic cancer metastasis by Yu-Ying Chao, Ruei-Ci Lin, Ping-Jui Su, Chu-An Wang, Ting-Yuan Tu, Ya-Chin Hou, Yi-Tzui Tsai, I-Chen Peng, Shaw-Jenq Tsai, Yan-Shen Shan, Chia-Yih Wang

“…Here, we demonstrated that melanophilin (MLPH) promotes PDAC metastasis by inducing the regrowth of primary cilia. Using RNA sequencing, we found that MLPH was upregulated in Gln-deficient conditions. …”
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The Combination of Host-Associated Bacillus megaterium R32 and Stachyose Promotes the Intestinal Health of Turbot (Scophthalmus maximus. L) by Yaoyao Kong, Sifan Zhao, Weihao Ou, Kangsen Mai, Yanjiao Zhang

“…The results showed that diets RS and RC significantly inhibited the expression of cell development and apoptosis-related genes (β-catenin, CyclinD1, BAX, Bid); diets RS and RC significantly increased the expression of intestinal tight junction protein claudin-4, while RS group significantly decreased the expression of myosin light chain kinase; diets RS and RC significantly decreased the expression of proinflammatory factors (IL-13, IL-15, IFN- γ), diet RS also significantly decreased the expression of TNF-α and AP-1, and increased the expression of TGF-β. 16s rRNA gene sequencing results showed that diets RS and RC significantly decreased the abundance of conditional pathogenic bacteria (Corynebacterium, Desulfovibrio, Escherichia-Shigella). …”
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Arabidopsis apoplast TET8 positively correlates to leaf senescence, and tet3tet8 double mutants are delayed in leaf senescence by Jayde A. Zimmerman, Benjamin Verboonen, Andrew P. Harrison Hanson, Luis R. Arballo, Judy A. Brusslan

“…The increase in apoplast TET8 in the pen1 mutant did not correspond to increased TET8 mRNA levels. In addition, apoplast TET8 was more abundant in the early leaf senescence myb59 mutant, meaning the increase in apoplast TET8 protein during leaf senescence is not dependent on pen1 or pen3. …”
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Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway by Chenlong Wang, Chuangjiang Chen, MengYa Wang, Sajid Ur Rahman, Bin Wei, Hongyan Ding, Wanyue Huang, Xichun Wang

“…When porcine ESCs were co-cultured with the addition of Rutin and ZEA following p53 gene silencing via siRNA transfection, Rutin significantly mitigated ZEA-induced mitochondrial damage, oxidative stress, and Fe2 + content through the p53 pathway. …”
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