Unexpected scarcity of ANME archaea in hydrocarbon seeps within Monterey Bay by A. C. Semler, A. E. Dekas

“…However, we observed atypical microbial communities: extremely few ANME sequences were detected in either 16S rRNA or <i>mcrA</i> gene surveys at Clam Field (<span class="inline-formula">&lt;</span> 0.1 % of total community reads), even after 6 months of incubation with methane in the laboratory, and only slightly more ANME sequences were recovered from Extrovert Cliff (<span class="inline-formula">&lt;</span> 0.3 % of total community reads). …”
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Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by Proteomics by Yimin Zhu, Xingyuan Xiao, Lairong Dong, Zhiming Liu

“…MicroRNAs are small noncoding RNA molecules that control expression of target genes. …”
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Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease by Masaki Fukui, Yoko Ogawa, Shin Mukai, Mizuka Kamoi, Teru Asato, Yutaka Kawakami, Kazuo Tsubota

“…Next, serial changes of tear secretion and VAMP8 expression at both protein and mRNA level of LG in an animal cGVHD model compared with the syngeneic control. …”
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Utilizing a novel fecal sampling method to examine resistance of the honey bee (Apis mellifera) gut microbiome to a low dose of tetracycline. by Casey L Gregory, Emma L Bradford, Richard D Fell, David C Haak, Lisa K Belden

“…We used metrics of alpha and beta diversity calculated from 16S rRNA gene amplicon sequences to compare gut community structure. …”
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Synergistic Effect of Hyperglycemia and Suppression on Adult Mouse Islet Beta Cell Replication by Szu-Tah Chen, Shin-Huei Fu, Samuel Hsu, Yu-Yao Huang, Brend Ray-Sea Hsu

“…gene silencing was performed by infecting islets of C57BL/6 with shRNA lentiviral particles. At 54 hours after viral infection, protein content in cultured targeting islets was 22% of that in freshly isolated islets. …”
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Acute Liver Failure Caused by Amanita phalloides Poisoning by Luca Santi, Caterina Maggioli, Marianna Mastroroberto, Manuel Tufoni, Lucia Napoli, Paolo Caraceni

“…Liver damage from Amanita phalloides is related to the amanitins, powerful toxins that inhibit RNA polymerase II resulting in a deficient protein synthesis and cell necrosis. …”
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Rapid Characterization and Action Mechanism of the Antidiabetic Effect of Diospyros lotus L Using UHPLC-Q-Exactive Orbitrap MS and Network Pharmacology by Shihan Qin, Mingjuan Liu, Sunv Tang, E. Shuai, Ziming Wang, Kaiquan Yu, Wei Cai

“…In addition, 92 relevant antidiabetic targets were mainly related to positive regulation of transcription from the RNA polymerase II promoter, extracellular space, and protein binding, suggesting the involvement of TNF, PI3K-Akt, and HIF-1 signaling pathways in the antidiabetic effect of Diospyros lotus L. …”
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Postmortem Interval Influences α-Synuclein Expression in Parkinson Disease Brain by Alexandra Dumitriu, Carlee Moser, Tiffany C. Hadzi, Sally L. Williamson, Christopher D. Pacheco, Audrey E. Hendricks, Jeanne C. Latourelle, Jemma B. Wilk, Anita L. DeStefano, Richard H. Myers

“…We examined expression of the full-length SNCA transcript (140 amino acid protein isoform), as well as total SNCA mRNA levels in 165 frontal cortex samples (101 PD, 64 control) using quantitative real-time polymerase chain reaction. …”
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Seed banking impacts native Acacia ulicifolia seed microbiome composition and function by Dylan Russell, Vaheesan Rajabal, Matthew Alfonzetti, Marlien M. van der Merwe, Rachael V. Gallagher, Sasha G. Tetu

“…In this study we investigated the composition and functional potential of seed-associated bacterial epiphytes associated with stored and freshly collected seeds of a native plant, Acacia ulicifolia, using both 16S rRNA gene sequencing and culture-based approaches. …”
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Association of Fecal Microbiota with Irritable Bowel Syndrome-Diarrhea and Effect of Traditional Chinese Medicine for Its Management by Fang Yang, Jiaqi Wu, Ning-Yuan Ye, Jing Miu, Jing Yan, Li-Na Liu, Bai Ye

“…In order to better elucidate the relationship between intestinal microbiota changes and IBS-D, we compared fecal microbiota of IBS-D rats and healthy control using pyrosequencing of bacterial 16S rRNA gene targeted. Furthermore, we explored the effects of different traditional Chinese medicine (TCM) on intestinal microbiota of IBS-D in dose-dependent manner. …”
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Network pharmacology study on the mechanism of berberine in Alzheimer’s disease model by Yaoyi Zhang, Shuai Lv, Pinyuan Huang, Lingmin Xiao, Nan Lin, En Huang

“…Further experiments demonstrated that BV2 and C8-D1A treated with BBR were decreased in the mRNA and protein expression of APP and presenilin 1 while PPARG was increased with a reduction in the NF-κB pathway. …”
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Effect of Ultrafine Powderization and Solid Dispersion Formation via Hot-Melt Extrusion on Antioxidant, Anti-Inflammatory, and the Human Kv1.3 Channel Inhibitory Activities of Ange... by Yunyao Jiang, Jingpei Piao, Nan Liu, Jincai Hou, Jianxun Liu, Weicheng Hu

“…In addition, AGN-SD inhibited the production of prostaglandin E2 and intracellular reactive oxygen species and the mRNA expression of inducible nitric oxide synthase, cyclooxygenase-2, interleukin 1β, and interleukin 6. …”
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Exploring Core Genes Associated with Sepsis and Systemic Inflammatory Response Syndrome Using Single-Cell Sequencing Technology by Shen Y, Leng L, Hu Y

“…The frequency of occurrence is on the rise, but there is a lack of certain indicators for the timely detection and recognition of illnesses.Methods: By virtue of scRNA-seq, this research has analyzed single-cell transcriptome data from samples taken from groups with septic death and systemic inflammatory response syndrome so as to identify the unique markers and patterns in immune response.Results: By revealing the status of twelve cell clusters of four major cell types in blood samples through UMAP cell clustering and the differences of major cell populations between the dead and SIRS patients, the results have elucidated the components of different cells and their marker genes in two disease states, and the response mechanism beneficial to disease diagnosis in blood samples.Conclusion: By establishing a theoretical framework centered on cellular and molecular regulation, the study has introduced a novel approach for diagnosing and treating sepsis death group and SIRS patients early, as well as differentiating and preventing these conditions.Keywords: sepsis, SIRS, single cell sequencing, biomarkers, single cell transcriptome…”
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Reevaluating the Concept of Treating Experimental Tumors with a Mixed Bacterial Vaccine: Coley’s Toxin by C. Maletzki, U. Klier, W. Obst, B. Kreikemeyer, M. Linnebacher

“…Quantitative gene expression analysis revealed upregulated mRNA levels of selected Toll-like receptors. Flow cytometric phenotyping of CT-stimulated huPBLs identified raised numbers of CD25+-activated leukocytes. …”
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Lessons Learned from Experimental Human Model of Zinc Deficiency by Ananda S. Prasad

“…The effect of zinc deficiency on thymulin activity and IL-2 mRNA was seen within eight to twelve weeks of the institution of zinc-deficient diet in human volunteers, whereas lymphocyte zinc decreased in 20 weeks and plasma zinc decreased in 24 weeks after instituting zinc-deficient diet. …”
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Rac1-dependent regulation of osteoclast and osteoblast differentiation by developmentally regulated GTP-binding 2 by Jung Ha Kim, Semun Seong, Kabsun Kim, Inyoung Kim, Jeong Woo Park, Jeong-Tae Koh, Nacksung Kim

“…Downregulation of Drg2 using siRNA in bone marrow-derived macrophages inhibited osteoclast differentiation and function and Rac1 activation in vitro. …”
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Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloprotein... by Szewczyk Karolina, Lewandowska Urszula, Owczarek Katarzyna, Sosnowska Dorota, Gorlach Sylwia, Koziołkiewicz Maria, Hrabec Zbigniew, Hrabec Elżbieta

“…To assess the effect of evening primrose extract on Caco-2 cell proliferation, crystal violet staining and sulforhodamine B (SRB) assays were used whereas mRNA expression and activity of MMPs were evaluated by RT-PCR and gelatin zymography.…”
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Anti-Inflammatory and Neuroprotective Role of Natural Product Securinine in Activated Glial Cells: Implications for Parkinson’s Disease by Dmitri Leonoudakis, Anand Rane, Suzanne Angeli, Gordon J. Lithgow, Julie K. Andersen, Shankar J. Chinta

“…Additionally, securinine also inhibited interferon-γ- (IFN-γ-) induced nitric oxide levels and iNOS mRNA expression. Furthermore, conditioned media (CM) from securinine pretreated BV2 cells significantly reduced mesencephalic dopaminergic neurotoxicity compared with CM from LPS stimulated microglia. …”
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Laser Capture Microdissection in Dentistry by Uraiwan Chokechanachaisakul, Tomoatsu Kaneko, Takashi Okiji, Reika Kaneko, Hideaki Suda, Jacques E. Nör

“…This paper is focused on the presentation and discussion of the existing literature that covers the fields of RNA analysis following LCM in dentistry.…”
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TMAO-Activated Hepatocyte-Derived Exosomes Are Widely Distributed in Mice with Different Patterns and Promote Vascular Inflammation by Xiang Liu, Jiazichao Tu, Ziqin Zhou, Bingxin Huang, Jianrong Zhou, Jimei Chen

“…Furthermore, TMAO-Exos, but not control-Exos, could significantly promote the mRNA expressions of Tnf, Icam1, Sele, and Cox-2 in the aorta. …”
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