Loss of YTHDC1 m6A reading function promotes invasiveness in urothelial carcinoma of the bladder by Jinyun Xu, Jonas Koch, Claudia Schmidt, Malin Nientiedt, Manuel Neuberger, Philipp Erben, Maurice Stephan Michel, Manuel Rodríguez-Paredes, Frank Lyko

“…Integrative analysis of multimodal sequencing datasets provided detailed insights into the molecular mechanisms mediating YTHDC1-dependent phenotypes and identified SMAD6 as a key transcript involved in the invasiveness of urothelial carcinoma of the bladder. Notably, SMAD6 mRNA colocalized less with YTHDC1 in tumoral tissues than in paratumoral tissues, indicating disrupted binding during cancer progression. …”
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A scoping review of evidence of naturally occurring Japanese encephalitis infection in vertebrate animals other than humans, ardeid birds and pigs. by Zoë A Levesque, Michael G Walsh, Cameron E Webb, Ruth N Zadoks, Victoria J Brookes

“…Direct evidence (virus, viral antigen or viral RNA) of JEV infection was identified in a variety of mammals and birds (not always identified to the species level), including bats, passerine birds (family Turdidae), livestock (cattle [Bos taurus] and a goat [Capra hircus]), carnivores (two meerkats [Suricata suricatta]), and one horse (Equus caballus). …”
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Avian Infectious Bronchitis Virus: Molecular Detection in Southwestern Ethiopia Chickens by Bezina Arega Emeru, Haregawi Tesfaye Desta

“…A side-by-side questionnaire was administered to assess risk factors. Total RNA was extracted, reverse transcription polymerase chain reaction (RT-PCR) was conducted, and products were visualized under UV light. …”
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Activation of PPAR𝛾 by Rosiglitazone Does Not Negatively Impact Male Sex Steroid Hormones in Diabetic Rats by Mahmoud Mansour, Elaine Coleman, John Dennis, Benson Akingbemi, Dean Schwartz, Tim Braden, Robert Judd, Eric Plaisance, Laura Ken Stewart, Edward Morrison

“…Treatment for eight weeks increased PPAR𝛾 mRNA and protein in the testis and elevated serum adiponectin, an adipokine marker for PPAR𝛾 activation. …”
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Screening and identification of purine degrading Lactobacillus fermentum 9-4 from Chinese fermented rice-flour noodles by Lianghua Lu, Tiantian Liu, Xiaoling Liu, Chenghua Wang

“…A new lactic acid bacteria strain designated as Lactobacillus fermentum 9-4 was screened out from 10 Shengzhamifen samples by a comprehensive method integrating agar plate selection, in vitro purine-metabolizing enzymatic activities of xanthine oxidase and urate oxidase, 16S rRNA gene sequencing and identification. The resting cells of L. fermentum 9-4 showed the maximum degradation rates of inosine and guanosine by respective 2.13 × 10−3 and 2.78 × 10−3 g/(L·min), and the highest assimilation ratio of guanosine by (55.93 ± 3.12)%, which are improvements over LAB strains characterized previously. …”
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Fecal shedding level of Haemonchus contortus is associated with gastrointestinal bacterial microbial composition in naturally infected sheep by Jeferson Vidart Ramos, Verônica Rocha Merched, Laura Dias da Silva Ribeiro, Guilherme Neves Lima Rattmann, Renan Eugênio Araújo Piraine, Fabio Pereira Leivas Leite

“…Stool samples were collected monthly for egg counts (EPG), and 16S rRNA gene sequencing was performed on five animals from each group. …”
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Apoptosis through Death Receptors in Temporal Lobe Epilepsy-Associated Hippocampal Sclerosis by Marcelo Ananias Teocchi, Lília D’Souza-Li

“…In addition, we evaluated the hippocampal relative mRNA expression of the two TNF receptors, TNFRSF1A and TNFRSF1B, in patients, being both upregulated (n=14; P<0.01 and P<0.04, resp.) when compared to the post mortem control group (n=4). …”
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Trichromacy is insufficient for mate detection in a mimetic butterfly by Andrew Dang, Gary D. Bernard, Furong Yuan, Aide Macias-Muñoz, Ryan I. Hill, J. P. Lawrence, Aline Giselle Rangel Olguin, Armando Luis-Martínez, Sean P. Mullen, Jorge Llorente-Bousquets, Adriana D. Briscoe

“…We examine the trichromatic visual system of A. fessonia and characterize its photoreceptors using RNA-seq, eyeshine, epi-microspectrophotometry, and optophysiology. …”
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VCAM-1 Promotes Angiogenesis of Bone Marrow Mesenchymal Stem Cells Derived from Patients with Trauma-Induced Osteonecrosis of the Femoral Head by Regulating the Apelin/CCN2 Pathway by Yiming Shao, Lei Sun, Baodong Ma, Ranran Jin, Yueyao Ban, Ruibo Li, Jianfa Wang, Hongkai Lian, Han Yue

“…VCAM-1 enhances the migration and angiogenesis of BMSCs. We further performed mRNA transcriptome sequencing to explore the mechanisms by which VCAM-1 promotes angiogenesis. …”
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Bimolecular Complementation to Visualize Filovirus VP40-Host Complexes in Live Mammalian Cells: Toward the Identification of Budding Inhibitors by Yuliang Liu, Michael S. Lee, Mark A. Olson, Ronald N. Harty

“…Virus-host interactions play key roles in promoting efficient egress of many RNA viruses, including Ebola virus (EBOV or “e”) and Marburg virus (MARV or “m”). …”
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Blood Transcriptional Signatures for Disease Progression in a Rat Model of Osteoarthritis by Michał Korostyński, Natalia Małek, Marcin Piechota, Katarzyna Starowicz

“…Functional links that connect expression of the gradually downregulated genes to the G13 signaling pathway were indicated. The mRNA abundance levels of the identified transcripts were further analyzed in publicly available gene expression dataset obtained from a GARP study cohort of OA patients. …”
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USING THE METHODS OF SYSTEMS BIOLOGY FOR PREDICTING PERSPECTIVE TARGET GENES TO SELECT C3 AND C4 CEREALS FOR OXIDATIVE STRESS RESISTANCE by A. V. Doroshkov, A. V. Bobrovskikh

“…Because these genes undergo the most conservative evolution and have the highest level of mRNA expression, we may assume that they contribute a lot to the antioxidant system functioning of the C3 and C4 cereal plants studied. …”
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FTO effects the proliferation, invasion, and glycolytic metabolism of colon cancer by regulating PKM2 by Kongyan Zhang, Fei Zhang, Jiahe Wang

“…In vitro experiments utilized five human colon cancer cell lines and a normal colon epithelial cell line, with Western blotting and RT-PCR for protein and mRNA quantification, respectively, and lentiviral transfection to modulate FTO expression. …”
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Genomic and Drug Resistance Profile of Bovine Multidrug-Resistant <i>Escherichia coli</i> Isolated in Kazakhstan by Pavel G. Alexyuk, Andrey P. Bogoyavlenskiy, Yergali S. Moldakhanov, Kuralay S. Akanova, Adolat N. Manakbayeva, Timur Kerimov, Vladimir E. Berezin, Madina S. Alexyuk

“…Whole-genome sequencing, followed by bioinformatic processing and the annotation of genes of this strain, showed that the genome has a total length of 4,803,482 bp and contains 4986 genes, including 122 RNA genes. A total of 31% of the genes are functionally significant and represent 26 functional groups. …”
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Analysis of ginseng rusty root symptoms transcriptome and its pathogenesis directed by reactive oxygen species theory by Pengcheng Yu, Xiaowen Song, Wei Zhang, Yao Yao, Junling Ren, Liyang Wang, Wenfei Liu, Zhaoping Meng, Xiangcai Meng

“…In this study, the phloem tissue of healthy Panax ginseng (AG), the nonred tissue of the phloem epidermis around the lesion (BG), and the red lesion site tissue of GRS (CG) were extracted for mRNA transcriptomic analysis; 35,958 differentially expressed genes (DEGs) were identified and were associated with multiple stress resistance pathways, reactive oxygen species (ROS), and iron ion binding. …”
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Is There a Real Relationship between the Presence of Helicobacter pylori in Dental Plaque and Gastric Infection? A Genotyping and Restriction Fragment Length Polymorphism Study on... by Mojtaba Moosavian, Elyas Kushki, Tahereh Navidifar, Eskandar Hajiani, Mahdi Mandegari

“…The presence of H. pylori was identified by the rapid urease test (RUT) and the amplification of ureAB and 16S rRNA genes. Also, to verify a hypothetical mouth-to-stomach infection route, the enzymatic digestions of three genes of cagA, vacA, and ureAB in H. pylori strains isolated from dental plaques and stomach samples were compared for each same case. …”
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Development and validation of a rapid loop-mediated isothermal amplification assay for the detection of Chrysomyxa and characterization of Chrysomyxa woroninii overwintering on Pic... by Wan Ting Yu, Xin Wang, Tan Yin, Clement Kin-Ming Tsui, Chong Juan You

“…In this study, a sensitive, genus-specific loop-mediated isothermal amplification (LAMP) assay targeting the ITS-28S rRNA region was developed to detect the presence of Chrysomyxa in spruce needle and bud samples. …”
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Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production by Duck Gyun Kim, Boncheol Gu, Yujin Cha, Jeonghan Ha, Yongjae Lee, Gahyeon Kim, Byung-Kwan Cho, Min-Kyu Oh

“…We also demonstrate improved secondary metabolite production using multiplexed genome editing with multiple single guide RNA libraries in several Streptomyces strains. Cas9-BD is also used to capture large BGCs using a developed in vivo cloning method. …”
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Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA by XiaoHong Zhang, YuJi Miao, XiaoDan Hu, Rui Min, PeiDang Liu, HaiQian Zhang

“…These results indicate that the zinc finger motif is a target domain for gamma radiation, which may decrease 5S rRNA expression via impairment of the zinc finger motifs in TFIIIA.…”
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Targeting Myeloid-Derived Suppressor Cells Is a Novel Strategy for Anti-Psoriasis Therapy by Chao Chen, Lirong Tan, Wu Zhu, Li Lei, Yehong Kuang, Panpan Liu, Jie Li, Xiang Chen, Cong Peng

“…Depleting MDSCs by Gemcitabine significantly suppresses IMQ-induced psoriatic inflammation and epidermal thickening as well as Th17 and Treg cell accumulation. Moreover, through the RNA-Seq technique, we validated some differentially expressed genes on CD4+ T-cells of IMQ-induced-MDSC-depleted mice such as IL-21 and Timd2, which are involved in Th17-cell differentiation or T-cell activation. …”
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