Comparative analysis of microbial contamination in diesel fuels using MALDI-TOF MS by Agnieszka Ludwiczak, Tomasz Zieliński, Ewelina Sibińska, Grażyna Czeszewska-Rosiak, Michał Złoch, Joanna Rudnicka, Andrzej Tretyn, Paweł Pomastowski

“…Detection and characterization of microbiological contamination in fuels were performed with further validation by 16 S rRNA gene sequencing. Microorganisms collected from three types of diesel fuel were cultured, and fuel samples were analyzed. …”
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Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-Inflammation by Wenpei Chen, Xiaolan Yao, Chenghao Zhou, Ziyang Zhang, Gang Gui, Baoqin Lin

“…Furthermore, DHK significantly downregulated protein expressions of VEGF and inducible NO synthase (iNOS) and mRNA expression of ICAM-1 in retinas. These results suggest that the antioxidative and anti-inflammatory activities of DHK may be important mechanisms involved in the protective effect of DHK on DR in STZ-induced diabetic rats.…”
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Investigating the Prognostic and Oncogenic Roles of Membrane-Associated Ring-CH-Type Finger 9 in Colorectal Cancer by Jiayan Zhang, Qinghan Jiao, Zhigang Chen

“…In vitro experiments involved siRNA-mediated MARCHF9 knockdown in COAD cell lines (SW480 and LoVo). …”
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Stalled disomes marked by Hel2-dependent ubiquitin chains undergo Ubp2/Ubp3-mediated deubiquitination upon translational run-off by Mario Scazzari, Ying Zhang, Anna Moddemann, Sabine Rospert

“…Eukaryotic cells utilize the ribosome-associated quality control (RQC) and no-go mRNA decay (NGD) pathways to resolve these problems. …”
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Lipopolysaccharide inhibits osteoblast formation and receptor activator of nuclear factor-κB ligand degradation via autophagy inhibition by Huaizhi Zhang, Jianhua Lin, Xu Chen, Jianhui Dai, Haibin Lin

“…Our results showed that LPS inhibited autophagy (LC3 conversion and autophagosome formation) and enhanced the protein and mRNA expression of RANKL in MC3T3-E1 osteoblast precursor line. …”
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Leptin Antagonizes Peroxisome Proliferator-Activated Receptor-γ Signaling in Growth Plate Chondrocytes by Lai Wang, Yvonne Y. Shao, R. Tracy Ballock

“…Leptin treatment also leads to a mild downregulation of PPAR mRNA expression and a significant MAPK/ERK-dependent PPARγ phosphorylation at serine 112/82. …”
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Empagliflozin Alleviates Hepatic Steatosis by Activating the AMPK-TET2-Autophagy Pathway in vivo and in vitro by Ting Li, Ting Fang, Linxin Xu, Xiangyang Liu, Xiaoyu Li, Mei Xue, Xiaochen Yu, Bei Sun, Liming Chen

“…Ten-eleven translocation 2 (TET2) knockdown was achieved by siRNA transfection. Hepatic steatosis was evaluated by Oil Red O staining and triglyceride quantification. …”
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The Effect of Therapeutic Blockades of Dust Particles-Induced Ca2+ Signaling and Proinflammatory Cytokine IL-8 in Human Bronchial Epithelial Cells by Ju Hee Yoon, Sung Hwan Jeong, Jeong Hee Hong

“…Our results show that exposure to several inhibitors of Ca2+ pathway attenuated the PM10-induced Ca2+ response and subsequent IL-8 mRNA expression. PM10-mediated Ca2+ signal and IL-8 expression were attenuated by several pharmacological blockades such as antioxidants, IP3-PLC blockers, and TRPM2 inhibitors. …”
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The urinary microbiome in association with diabetes and diabetic kidney disease: A systematic review. by Tiscar Graells, Yi-Ting Lin, Shafqat Ahmad, Tove Fall, Johan Ärnlöv

“…Most of the studies had a small sample size, used voided midstream urine, and used 16S rRNA sequencing.<h4>Conclusion</h4>This systematic review summarizes trends seen throughout published data available to have a first baseline knowledge of the urinary microbiome, and its microbiota, in association with diabetes including the decreased richness and α-diversity in urinary microbiota in individuals with diabetes compared to healthy controls and the decreased α-diversity with the evolution of kidney disease independently of the cause.…”
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Genome-wide identification and analysis of the NF-Y transcription factor family reveal its potential roles in tobacco (Nicotiana tabacum L.) by Zhen Tian, Luyao Xue, Jincun Fu, Wenting Song, Baojian Wang, Jinhao Sun, Xiujiang Yue, Fanrui Cheng, Jingjing Mao, Jiangtao Chao, Dawei Wang, Shaopeng Li

“…Their tissue specificity and expression pattern analyses for leaf development, drought and saline-alkali stress, and ABA response were carried out using RNA-seq or qRT-PCR. These findings illuminate the role of NtNF-Ys in regulating plant leaf development, drought and saline-alkali stress tolerance, and ABA response. …”
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Alternative Splicing of Cdh23 Exon 68 Is Regulated by RBM24, RBM38, and PTBP1 by Nana Li, Haibo Du, Rui Ren, Yanfei Wang, Zhigang Xu

“…Exon 68 of the Cdh23 gene is spliced into its mRNA only in a few cell types including hair cells. …”
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CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata by Mingjia Yu, Shanjun Yang, Hongxia Sun, Qiang Xia

“…Moreover, temporal expression profile analysis demonstrates a drastic induction in the expression of CD63 in hemocytes after pathogenic challenge with either V. parahaemolyticus or V. alginolyticus. By performing dsRNA-mediate RNAi knockdowns of CD63, a dramatic reduction in hemocytes phagocytic activity to pathogenic Vibrio is recorded by flow cytometry, revealing the definite role of Pu-CD63 in promoting hemocyte-mediated phagocytosis. …”
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Studi Awal Analisis Molekuler Human Papillomavirus dari Apusan Glans dan Batang Penis by Maya Savira, Resty Yuwandari, Yossi Maryanti, Rahmat Azhari Kemal, Donel S

“…Hasil: Optimasi awal menunjukkan gen β-globin dapat terdeteksi dari hasil ekstraksi dengan kit Zeesan Viral RNA Extraction. Pita HPV hasil PCR dengan primer MY09 dan MY11 dapat muncul namun masih tipis. …”
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Ataxin-10 Inhibits TNF-α-Induced Endothelial Inflammation via Suppressing Interferon Regulatory Factor-1 by Yong Li, Qi Zhang, Na Li, Liting Ding, Jinping Yi, Yue Xiao, Shibiao Chen, Xuan Huang

“…Overexpression of Ataxin-10 markedly inhibited the mRNA expressions of VCAM-1 and several cytokines including MCP-1, CXCL-1, CCL-5, and TNF-α; thus, it can also suppress monocyte adhesion to endothelial cells. …”
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Thioflavin-T: application as a neuronal body and nucleolar stain and the blue light photo enhancement effect by Jin-Hong Min, Heela Sarlus, Sho Oasa, Robert A. Harris

“…Furthermore, we show that THT could potentially be used as a an alternative to the use of fluorescent reporters or other more costly RNA binding compounds in the study of nucleolar dynamics owing to its ability to clearly stain nucleoli in live cells. …”
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Treatment with Herbal Formula Extract in the hSOD1G93A Mouse Model Attenuates Muscle and Spinal Cord Dysfunction via Anti-Inflammation by Eun Jin Yang, Sun Hwa Lee, Mudan Cai

“…To evaluate the effects of HFE, we performed footprint behavioral tests, western blotting, and immunohistochemistry to detect protein expression and quantitative PCR to detect mRNA levels in the muscles and spinal cord of hSOD1G93A mice. …”
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Groundbreaking Technologies and the Biocontrol of Fungal Vascular Plant Pathogens by Carmen Gómez-Lama Cabanás, Jesús Mercado-Blanco

“…Other technologies such as genome editing, RNA interference (RNAi), and functional peptides can enhance BCA efficacy against pathogenic fungi. …”
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Ethanol Disrupts the Protective Crosstalk Between Macrophages and HBV-Infected Hepatocytes by Murali Ganesan, Anup S. Pathania, Grace Bybee, Kusum K. Kharbanda, Larisa Y. Poluektova, Natalia A. Osna

“…We found that exposure of HepG2.2.15 cells to an acetaldehyde-generating system (AGS) increased HBV RNA, HBV DNA, and cccDNA expressions and suppressed the activation of ISGs, <i>APOBEC3G</i>, <i>ISG15</i>, and <i>OAS1</i>. …”
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Insight into the Advances in Clinical Trials of SARS-CoV-2 Vaccines by Syed Mohammed Basheeruddin Asdaq, Shahamah Jomah, Syed Imam Rabbani, Ali Musharraf Alamri, Salman Khalaf Salem Alshammari, Badr Sami Duwaidi, Majed Sadun Alshammari, Abdulhakeem S. Alamri, Walaa F. Alsanie, Majid Alhomrani, Nagaraja Sreeharsha, Mohd. Imran

“…Among them are BioNTech and Moderna vaccines (mRNA based); ChAdOx1, Gam-COVID-Vac, Janssen vaccines (vector-based); CoronaVac, COVAXIN (virus inactivated); and EpiVacCorona vaccine (viral peptide). …”
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circSPECC1 Promotes Proliferation and Migration of LNCaP Prostate Cancer Cells by Affecting Their Epithelial-Mesenchymal Transition by Fangmei Xie, Jian Shen, Zeping Han, Wenfeng Luo, Liyin Liao, Jinhua He

“…Methods. Stable circSPECC1 shRNA-expressing and circSPECC1-overexpressing LNCaP cell lines were constructed, and relative gene expression levels were determined by RT-PCR. …”
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