Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma by Zhaoming Lu, Kezheng Peng, Ning Wang, Hong-Min Liu, Guiqin Hou

“…The results showed that, after downregulating the expression of p70S6K and p-p70S6K by p70S6K siRNA, the inhibitory effects of rapamycin on cell proliferation, cell cycle, and tumor growth were significantly enhanced in vitro and in vivo. …”
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Targeting T-Cell Activation for Malaria Immunotherapy: Scoping Review by Balsa Nobility Gustifante, Shafia Khairani, Nisa Fauziah, Silvita Fitri Riswari, Afiat Berbudi

“…The findings underscore the potential of T cells, particularly CD8+ T cells, in liver-stage defense and advocate for the exploration of advanced vaccine platforms and novel therapies, such as mRNA-based vectors and monoclonal antibodies.…”
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Microscopical and molecular investigation of Ichthyophthirius multifiliis from fish in Mosul city, Iraq by Esraa A. Altaie, Nadia N. Alhayali

“…Molecular phylogenetic analysis conducted with 18S rRNA gene confirming species identification of one positive isolate out of 13 belonging to the ectoparasite I. multifiliis under the accession number PQ012981 and according to the genetic tree analysis of I. multifiliis showed 100% match with each of the United States isolates for the same species and under the accession numbers KJ690571, KJ690572, KJ690570, KJ690568, KJ690567, KJ690566 and KJ690565 and isolates OM865867 in India and OM302501 in China. …”
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Evaluation of insertion and deletion polymorphisms of Brazilian Odontoceti by Dafne Adriana Abreu dos Anjos, Anna Luiza dos Santos Donato, Rodrigo Goldenberg-Barbosa, Elizeu Fagundes de Carvalho, Cesar Rogerio Leal do Amaral

“…In this study, we performed an in silico analysis of InDels in 16S rRNA from 27 species of toothed whales, dolphins, and porpoises present in Brazilian waters and recurrently found stranded in the region. …”
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Molecular genetic methods for intestinal microbiome investigation by O. L. Kardymon, A. V. Kudryavtseva

“…Meanwhile, today, to distinguish species and strains of microorganisms at DNAs or RNA level, standard culture and visual method are combined to molecular biology and genetics methods even more frequently. …”
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Dysregulated Expression and Methylation Analysis Identified TLX1NB as a Novel Recurrence Marker in Low-Grade Gliomas by Hongzhou Duan, Zuozhen Yang, Chen Li, Jiayong Zhang, Shengli Shen, Changwei Yuan, Yingjin Wang

“…In this study, we integrated the TCGA and GEO datasets, analyzing RNA and methylation data for recurrence (R) and nonrecurrence (NR) groups. …”
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IgG expressed by renal tubular epithelial cells in epithelial mesenchymal transformation and interstitial fibrosis in diabetic kidney disease by Xinyao Wang, Zhenling Deng, Yue Wang

“…High glucose induced an over expression of IgG in human renal tubular epithelial cells, and knockdown of IgG with siRNA relieved the expression of α-smooth muscle actin (SMA), collagen IV, fibronectin, and transforming growth factor (TGF)-β1 under high glucose conditions. …”
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Bilateral acute macular neuroretinopathy following influenza A infection by Charles W. Ryan, Rajesh C. Rao

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Role of PPARG in Chemosensitivity-Regulating Network for Hypopharyngeal Squamous Cell Carcinoma by Fanyong Kong, Boxuan Han, Jiaming Chen, Xixi Shen, Lizhen Hou, Jugao Fang, Meng Lian

“…Here, we first employed RNA expression data analysis and literature data mining to uncover candidate genes related to HSCC chemosensitivity. …”
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A comprehensive review of mycoviruses infecting the plant pathogenic fungus Rosellinia necatrix by Sidra Hassan, Robert H. A. Coutts, Atif Jamal, Ioly Kotta-Loizou, Ioly Kotta-Loizou

“…The white root rot fungus is found to harbor diverse mycoviruses with double-stranded and positive-sense single-stranded RNA genomes from different families, including Spinareoviridae, Megabirnaviridae, Partitiviridae, Quadriviridae, Pseudototiviridae, Endornaviridae, Fusariviridae, Yadokariviridae, Hypoviridae, Fusagraviridae and Megatotiviridae. …”
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Morphine in Combination with Ketamine Improves Cervical Cancer Pain and Suppresses Immune Function via the JAK3/STAT5 Pathway by Yurong Jiang, Tong Li, Yi Qian, Xiaoming Zuo, Jinmei Liu

“…The levels of interferon (IFN)-γ, interleukin (IL)-2, and IL-17 and the corresponding mRNA expression in vitro were determined using ELISA and qRT-PCR, respectively. …”
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Gemcitabine combined with baicalein exerts antiviral activity against PEDV by inhibiting the entry and replication phases by Yingge Zheng, Yanfei Zhang, Fangying Xiong, Dehua Luo, Yuxiang Wang, Yijia Zhang, Dengguo Wei

“…Porcine epidemic diarrhea virus (PEDV) is a member of the Coronaviridae family, whose genome consists of a single strand of positive-sense RNA and has evolved into multiple epidemic lineages with no available drugs in clinical practice. …”
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Use of Posttranscription Gene Silencing in Squash to Induce Resistance against the Egyptian Isolate of the Squash Leaf Curl Virus by Omnia Taha, Inas Farouk, Abdelhadi Abdallah, Naglaa A. Abdallah

“…Two genes fragment from SqLCV introduced in sense and antisense orientations using pFGC5049 vector to be expressed as hairpin RNA. The first fragment was 348 bp from replication associated protein gene (Rep). …”
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CAR T cells for treating autoimmune diseases by Dimitrios Mougiakakos, Ulrike Koehl, Stephan Fricke, Ulrich Blache, Sandy Tretbar

“…Finally, we will address safety and manufacturing aspects for CAR T cells and discuss mRNA technologies and automation concepts for ensuring the future availability of safe and efficient CAR T cell products.…”
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Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample by Pankaj Kumar Arora, Alok Srivastava, Vijay Pal Singh

“…These eighteen 4C2NP-decolorizing strains have been identified as members of four different genera, including Bacillus, Paenibacillus, Pseudomonas, and Leuconostoc based on the 16S rRNA gene sequencing and phylogenetic analysis. Most of the bacteria (10) belonged to the genus Bacillus and contributed 56% of the total 4C2NP-degrading bacteria, whereas the members of genera Paenibacillus and Pseudomonas represented 22% and 17%, respectively, of total 4C2NP-degrading isolates. …”
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HOXA-AS3 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells via the miR-455-5p/PD-L1 Axis by Cheng Zeng, Shaojun Ye, Yu Chen, Qu Zhang, Yan Luo, Liang Gai, Bo Luo

“…Thus, the present study is aimed at elucidating the functions of the lncRNA HOXA-AS3 in HCC. The functions of the HOXA-AS3/miR-455-5p/programmed death-ligand 1 (PD-L1) axis were investigated in vitro via qRT-PCR and dual-luciferase reporter assays. …”
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Chemokine Expression in Human Astrocytes in Response to Shiga Toxin 2 by Naomi Kioka, Koichi Minami, Akira Tamura, Norishige Yoshikawa

“…Expression of both interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) mRNA in HASTs was significantly upregulated by Stx2. …”
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Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application by Nuria Nieto-Nicolau, Eva M. Martínez-Conesa, Ricardo P. Casaroli-Marano

“…We evaluated graft quality in terms of percent of p63-positive (p63+) cells by immunofluorescence, colony forming efficiency, and mRNA and protein expression of p63, PAX6, Wnt7a, E-cadherin, and cytokeratin (CK) 12, CK3, and CK19. …”
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Molecular techniques for cancer diagnostics by Vimal Kishor Singh, Ramesh Chandra

“…Several key findings are being identified to discover and validate such molecular biomarkers for their diagnostic potential at the DNA, RNA, and protein levels. Furthermore, noninvasive imaging technologies and serum biomarkers are promising higher quality outputs in a limited time which could be a useful tool to monitor early responses to the treatment and may be able to design the remedial course. …”
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Blocking REDD1/TXNIP Complex Ameliorates HG-Induced Renal Tubular Epithelial Cell Apoptosis and EMT through Repressing Oxidative Stress by Lin Mu, Nan Chen, Yakun Chen, Zhifen Yang, Huandi Zhou, Shan Song, Yonghong Shi

“…Similarly, the transfection of REDD1 siRNA plasmid also suppressed HG-induced apoptosis and EMT. …”
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