Azithromycin Promotes the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells after Stimulation with TNF-α by Tingting Meng, Ying Zhou, Jingkun Li, Meilin Hu, Xiaomeng Li, Pingting Wang, Zhi Jia, Liyu Li, Dayong Liu

“…Real-time quantitative polymerase chain reaction (RT-qPCR) analysis was performed to detect the mRNA expression of osteogenic-related genes, including RUNX2, OCN, and BSP. …”
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The Role of β-Catenin in Th1 Immune Response against Tuberculosis and Profiles of Expression in Patients with Pulmonary Tuberculosis by Kunlong Xiong, Jinxia Niu, Ruijuan Zheng, Zhonghua Liu, Yanzheng Song, Lin Wang, Changtai Zhu, Lin Fan

“…Four weeks after infection, the mRNA expression of IFN-γ, TNF-α, and TCF-7 in the lungs of mice was measured. …”
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Optimized Method to Generate Well-Characterized Macrophages from Induced Pluripotent Stem Cells by Qimin Hai, Peter Bazeley, Juying Han, Gregory Brubaker, Jennifer Powers, Claudia M. Diaz-Montero, Jonathan D. Smith

“…The first three principal components of the RNA sequencing data showed clear clustering by differentiation stage. …”
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Vinblastine resets tumor-associated macrophages toward M1 phenotype and promotes antitumor immune response by Jing Wang, Jin Wang, Yi-Na Wang, Yuan-Yuan Wang, Wen-Juan Bai, Nai-Jun Miao

“…VBL and anti-PD-1 combination therapy was then investigated in comparison with monotherapy. RNA-seq of TAM of treated and untreated with VBL was performed to explore the changes in pathway activities. siRNA mediated knockdown experiments were performed to verify the target pathway that was affected by VBL treatment.Results Here, we showed that VBL, an antineoplastic agent that destabilizes microtubule, drove macrophage polarization into the M1-like phenotype both in vitro and in tumor models. …”
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Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension by Xiao‐ting Tian, Zhou‐yang‐fan Peng, Yu‐si Wu, Yuan‐yuan Cao, Xue‐chun Li, Ying Li, Si‐Yuan Tang, Alex F. Chen, Xiao‐hui Li

“…METHODS AND RESULTS NLRP3 knockout or short hairpin RNA interference of GSDME was performed in PAH animal models to investigate its effect on PAH progression. …”
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Effects of supplementation with vitamin D3 on growth performance, lipid metabolism and cecal microbiota in broiler chickens by Jiawei Li, Ximei Li, Jiamin Tian, Linna Xu, Yan Chen, Susu Jiang, Guohua Zhang, Jianxiong Lu

“…However, mRNA levels of genes involved in DNL and cellular lipid uptake (LPL and FATP1) and LPL activity were decreased in abdominal adipose tissue, and that of genes involved in lipid oxidation and lipolysis (HSL and ATGL) was increased by VD3. …”
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PET Study of Sphingosine-1-phosphate Receptor 1 Expression in Response to S. aureus Infection by Hao Jiang, Jiwei Gu, Haiyang Zhao, Sumit Joshi, Joel S. Perlmutter, Robert J. Gropler, Robyn S. Klein, Tammie L. S. Benzinger, Zhude Tu

“…NIBR-0213 or S1PR1-specific DsiRNA pretreatment reduced the tissue uptake of [18F]TZ4877, suggesting that uptake of [18F]TZ4877 is specific. …”
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Desmoglein-2 was a novel cancer-associated fibroblasts-related biomarker for oral squamous cell carcinoma by Jin Liu, Zhonghao Sun, Shihui Cao, Hao Dai, Ze Zhang, Jingtao Luo, Xudong Wang

“…Our object is to explore a CAFs-related biomarker in OSCC. Methods Single-cell RNA sequencing (ScRNA-seq) analysis was used to pinpoint CAF clusters in OSCC samples. …”
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<i>Artemia</i> Nauplii Enriched with Soybean Lecithin Enhances Growth Performance, Intestine Morphology, and Desiccation Stress Resistance in Yellow Drum (<i>Nibea albiflora</i>) L... by Zhenya Zhou, Pian Zhang, Peng Tan, Ruiyi Chen, Weihua Hu, Ligai Wang, Yuming Zhang, Dongdong Xu

“…This growth improvement was due to enhanced intestinal morphology, characterized by a significantly higher mucosal fold height, microvillus density, and microvillus length (<i>p</i> < 0.05). Intestinal RNA sequencing identified 160 upregulated and 447 downregulated differentially expressed genes (DEGs) in the SL group compared to the NH group. …”
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Dichlormid protect wheat from fomesafen residual injury by increasing PPO expression and the photosynthesis characterize by Dongzhi Li, Yilin Liu, Li Xu, Hao Yu, Yunchao Kan, Runqiang Liu, Guangling Li

“…To explore its molecular mechanism, RNA-Seq was conducted to analysis transcript profiles between fomesafen and fomesafen+dichlormid treated wheat seedlings. …”
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Prevalence of Hepatitis C Virus Among Hospitalized Patients in a Tertiary Hospital in Italy: The Basis for a National Screening Assessment Model? by Giulia Morsica, Massimo Locatelli, Gema Hernandez-Ibarburu, Francesca Rusconi, Alba Segovia-Hilara, Davide Di Napoli, Matteo Moro, Salvatore Mazzitelli, Hamid Hasson, Federico Esposti, Roberts Mazzuconi, Antonella Castagna

“…Among 1297 HCV Ab positive PTs, only 198 (15.3%) were tested for the presence of HCV RNA. The birth cohort 1969–1989 had a modest seroprevalence (1.5%), yet they were the most affected age group, with 44.4% being HCV RNA positive. …”
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纳米孔长读测序鉴定肝癌细胞中低氧特异性转录本 by 高力, 沈晟齐, 高平

“…方法实验选取正常肝实质细胞系THLE-3与肝癌细胞系Hep3B作为研究对象，分别置于常氧与低氧条件下培养48 h后，提取其总RNA。随后，采用纳米孔测序技术，对这两种细胞系在不同氧浓度下的转录组进行高通量、高精度的测序分析。…”
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Expression of Toll-Like Receptors 2 and 4 and Related Cytokines in Patients with Hepatic Cystic and Alveolar Echinococcosis by Tuerhongjiang Tuxun, Hai-Zhang Ma, Shadike Apaer, Heng Zhang, Amina Aierken, Yu-Peng Li, Ren-Yong Lin, Jin-Ming Zhao, Jin-Hui Zhang, Hao Wen

“…Median levels of TLR2 mRNA in AE and CE groups were significantly elevated as compared with that in healthy control group. …”
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Recognition of HER2 expression in hepatocellular carcinoma and its significance in postoperative tumor recurrence by Ji‐Hua Shi, Wen‐Zhi Guo, Yang Jin, Hua-Peng Zhang, Chun Pang, Jie Li, Pål‐Dag Line, Shui‐Jun Zhang

“…HER2 in HCC biology needs further explorations. Methods Clinical and mRNA data of HCC patients were obtained from TCGA HCC cohort, GSE89377 and GSE115018. …”
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Nuclear talin-1 provides a bridge between cell adhesion and gene expression by Alejandro J. Da Silva, Hendrik S.E. Hästbacka, Mikael C. Puustinen, Jenny C. Pessa, Jens C. Luoto, Erika Sundström, Benjamin T. Goult, Guillaume Jacquemet, Eva Henriksson, Lea Sistonen

“…Importantly, small interfering RNA (siRNA)-mediated depletion of endogenous TLN1 triggers extensive changes in the gene expression profile of human breast epithelial cells. …”
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Insulin-Attenuated Inflammatory Response of Dendritic Cells in Diabetes by Regulating RAGE-PKCβ1-IRS1-NF-κB Signal Pathway: A Study on the Anti-Inflammatory Mechanism of Insulin in... by Liding Zhao, Ya Li, Qingbo Lv, Min Wang, Yi Luan, Jiale Song, Guosheng Fu, Junbo Ge, Yunzeng Zou, Wenbin Zhang

“…The fluctuations in the RNA levels of cytokines and chemokines were analyzed by quantitative RT-PCR. …”
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Genome-Wide Expression Profiles of Hemp (Cannabis sativa L.) in Response to Drought Stress by Chunsheng Gao, Chaohua Cheng, Lining Zhao, Yongting Yu, Qing Tang, Pengfei Xin, Touming Liu, Zhun Yan, Yuan Guo, Gonggu Zang

“…In order to decipher the molecular responses of hemp to drought stress, transcriptome changes of drought-stressed hemp (DS1 and DS2), compared to well-watered control hemp (CK1 and CK2), were studied with RNA-Seq technology. RNA-Seq generated 9.83, 11.30, 11.66, and 11.31 M clean reads in the CK1, CK2, DS1, and DS2 libraries, respectively. …”
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Plastome data provides new insights into population differentiation and evolution of Ginkgo in the Sichuan Basin of China by Liyun Nie, Fangling Liu, Meixia Wang, Zhuying Jiang, Jiali Kong, Luke R. Tembrock, Shenglong Kan, Penghao Wang, Jie Wang, Zhiqiang Wu, Xiaoli Liu

“…We identified 170 sequence variants and extensive RNA editing (235 sites) with a bias toward hydrophobic amino acid conversions, suggesting active molecular evolution. …”
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Plastid genome comparison and phylogenetic analyses of the Chinese group of medicinal species and related taxa within Asparagus genus by Pingxuan Xie, Tinglu Wang, Jiawei Tan, Linyuan Fan, Changkun Liu, Hanjing Yan

“…However, accurate authentication of these medicinal species and their phylogenetic relationships with related taxa remains unresolved.MethodsTo identify simple sequence repeats (SSRs) and divergence hotspot regions appropriate for future authentication studies, as well as to infer the phylogenetic relationships among Asparagus species, we employed a plastid genome (plastome) dataset consisting of 25 Asparagus species (21 newly sequenced and four retrieved from GenBank), encompassing 12 Chinese medicinal species, for comparative and phylogenetic analyses.ResultsAll Asparagus plastomes displayed a typical quadripartite structure with sizes ranging from 155,948 bp to 157,128 bp and harbored 114 unique genes (80 protein-coding genes, 30 tRNA genes, and four rRNA genes). IRscope and Mauve analyses indicated minimal structural variation among Asparagus plastomes. …”
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Preoperative liquid biopsy transcriptomic panel for risk assessment of lymph node metastasis in T1 gastric cancer by Ping’an Ding, Jiaxiang Wu, Haotian Wu, Wenqian Ma, Tongkun Li, Peigang Yang, Honghai Guo, Yuan Tian, Jiaxuan Yang, Limian Er, Renjun Gu, Lilong Zhang, Ning Meng, Xiaolong Li, Zhenjiang Guo, Lingjiao Meng, Qun Zhao

“…This model significantly reduced overtreatment rates from 83.9 to 44.1% in tissue specimens and from 84.4 to 56.0% in liquid biopsies. The 4-mRNA panel showed specificity for T1 GC compared to other gastrointestinal cancers (P < 0.001). …”
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