Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in mice by Xiaoyan Duan, Xiaoyan Duan, Xiaoyan Duan, Xiaoyan Duan, Rilige Wu, Jianbo Li, Jianbo Li, Zeya Li, Yanqi Liu, Ping Chen, Bangmao Wang, Bangmao Wang, Bangmao Wang

“…RT-qPCR and Western blotting were used to detect mRNA and protein expression of target genes. The fecal microbiota was analyzed by 16S rRNA sequencing. …”
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Inhibitory Mechanism of Robiotic Bacteria on The Growth of Vibrio harveyi in Tiger Shrimp (Penaeus monodon) Larvae by . Widanarni, E. Ayuzar, . Sukenda

“…Partial sequencing of 16S-rRNA gene showed that 1Ub was similar to Pseudoalteromonas piscicida, whereas SKT-b and Ua were similar to Vibrio alginolyticus. …”
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Transcriptomic analysis of Rhipicephalus microplus hemocytes from female ticks infected with Babesia bovis or Babesia bigemina by Rubikah Vimonish, Janaina Capelli-Peixoto, Wendell Johnson, Lowell Kappmeyer, Perot Saelao, Naomi Taus, Chungwon Chung, Massaro Ueti

“…Hemocyte cells were collected from replete female ticks infected with Babesia bovis or Babesia bigemina to perform high-throughput RNA-sequencing (RNA-Seq) analysis. Results This study identified major changes in the gene profile of tick hemocytes during Babesia infection. …”
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Exosomes Play an Important Role in the Progression of Plasma Cell Mastitis via the PI3K-Akt-mTOR Signaling Pathway by Xiaohong Wang, Yong Han, Jian Liu, Yingzhe Zhang, Kai Cheng, Jiwei Guo, Qingqun Guo, Song Liu, Hongguang Sun, Yitong Hua, Guoqiang Zhang, Shujian Xu, Fengli Guo, Zhenlin Yang

“…The transcriptome expression difference of exosomes was detected by RNA-Seq; then, we confirmed the key difference genes by western blot and immunohistochemistry. …”
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Dorsal Root Ganglion Maintains Stemness of Bone Marrow Mesenchymal Stem Cells by Enhancing Autophagy through the AMPK/mTOR Pathway in a Coculture System by Shuaishuai Zhang, Junqin Li, Huijie Jiang, Yi Gao, Pengzhen Cheng, Tianqing Cao, Donglin Li, Jimeng Wang, Yue Song, Bin Liu, Hao Wu, Chunmei Wang, Liu Yang, Guoxian Pei

“…In addition, AMPK inhibitor compound C could significantly downregulate the protein expression of LC3 and the mRNA level of stemness genes in the coculture group. …”
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Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis by Leonidas Kandilogiannakis, Eirini Filidou, Ioannis Drygiannakis, Gesthimani Tarapatzi, Stylianos Didaskalou, Maria Koffa, Konstantinos Arvanitidis, Giorgos Bamias, Vassilis Valatas, Vasilis Paspaliaris, George Kolios

“…Similarly, HIOs elicited strong mRNA and protein mesenchymal (CXCL10) and epithelial (CXCL1, CCL2, CXCL8, and CCL20) chemokine responses in early but not late passages. …”
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Integrated analysis of cell cycle and p53 signaling pathways related genes in breast, colorectal, lung, and pancreatic cancers: implications for prognosis and drug sensitivity for... by Jiyauddin Khan, Priyanjana Ghosh, Urmi Bajpai, Kountay Dwivedi, Daman Saluja

“…Validation of hub genes at mRNA and protein levels was done via GEPIA2 and Human Protein Atlas. …”
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Inhibition of the Ubiquitin-Activating Enzyme UBA1 Suppresses Diet-Induced Atherosclerosis in Apolipoprotein E-Knockout Mice by Jiawei Liao, Xiaolei Yang, Qiuyue Lin, Shuang Liu, Yunpeng Xie, Yunlong Xia, Hui-Hua Li

“…Besides, PYR-41 also suppressed aortic mRNA expression of NADPH oxidase (Nox1, Nox2, and Nox4) and lesional oxidative stress levels, determined by DHE staining. …”
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Lactoferrin Induces Osteoblast Growth through IGF-1R by Jian-Ming Hou, En-Yu Chen, Fan Lin, Qing-Ming Lin, Ying Xue, Xu-Hua Lan, Man Wu

“…Results. (1) 1, 10, 100, and 1000 μg/mL lactoferrin induced the expression of IGF-1 mRNA and protein. 10 μg/mL and 100 μg/mL lactoferrin induced the expression of IGF-1R mRNA and protein. (2) Lactoferrin (100 μg/mL) induced osteoblast proliferation while inhibiting apoptosis. …”
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IL-33 Deficiency Attenuates Lung Inflammation by Inducing Th17 Response and Impacting the Th17/Treg Balance in LPS-Induced ARDS Mice via Dendritic Cells by Li Cheng, Yang Jiao, Wei Jiang, Xin Zhang, Liping Zhang, Gongwei Jia

“…Furthermore, depletion of IL-33 inhibited Th17 cells, significantly decreased RORγt mRNA and protein expression and IL-17 levels in BALF, and led to less differentiation of T cells into Th17 cells than Treg cells. …”
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Metallothioneins 1 and 2 Modulate Inflammation and Support Remodeling in Ischemic Cardiomyopathy in Mice by Georg D. Duerr, Daniela Dewald, Eva J. Schmitz, Luise Verfuerth, Katharina Keppel, Christine Peigney, Alexander Ghanem, Armin Welz, Oliver Dewald

“…Hearts were examined with M-mode echocardiography and processed for histological and mRNA studies. Results. Expression of MT1/2 mRNA was transiently induced during repetitive I/R in WT-mice, accompanied by a transient inflammation, leading to interstitial fibrosis with left ventricular dysfunction without infarction. …”
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Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China by Haixiang Qiu, Patrick John Kelly, Jilei Zhang, Qinghua Luo, Yi Yang, Yongjiang Mao, Zhangping Yang, Jing Li, Hongzhuan Wu, Chengming Wang

“…(JX629807) were present in ruminants from China, while the 16S rRNA FRET-qPCR sequence data indicated that there might also be A. platys, A. phagocytophilum, Anaplasma sp. …”
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Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and Adrenocorticotropin by Ivan Igaz, Gábor Nyírő, Zoltán Nagy, Henriett Butz, Zsolt Nagy, Pál Perge, Peter Sahin, Miklós Tóth, Károly Rácz, Peter Igaz, Attila Patócs

“…Expression of microRNAs was studied in plasma samples of 10 individuals examined by 1 mg dexamethasone suppression test and another 10 individuals stimulated by 250 μg tetracosactide (adrenocorticotropin). Total RNA was isolated and microRNA expression was analyzed by real-time reverse transcription quantitative polymerase chain reaction normalized to cel-miR-39 as reference. …”
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Effect of High, Medium, and Low Molecular Weight Hyaluronan on Inflammation and Oxidative Stress in an In Vitro Model of Human Nasal Epithelial Cells by Giusy Daniela Albano, Anna Bonanno, Luca Cavalieri, Eleonora Ingrassia, Caterina Di Sano, Liboria Siena, Loredana Riccobono, Rosalia Gagliardo, Mirella Profita

“…We evaluated the ERK1/2 and IκBα phosphorylation, NF-κB signal pathway activation, ROS production, IL-8 and NOX-4 protein, and mRNA levels, in nasal epithelial cells RPMI 2650 stimulated with recombinant human (rh) IL-17A. …”
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Scapular Angiomatoid Fibrous Histiocytoma with EWSR1-CREB1 Fusion in an Adult Patient by Hiroshi Kobayashi, Naohiro Makise, Aya Shinozaki-Ushiku, Yuki Ishibashi, Masachika Ikegami, Shinji Kohsaka, Tetsuo Ushiku, Katsutoshi Oda, Kiyoshi Miyagawa, Hiroyuki Aburatani, Hiroyuki Mano, Sakae Tanaka

“…Comprehensive genomic analysis, especially RNA sequencing, is efficient in diagnosing this rare tumor. …”
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TEAD1‐Mediated Trans‐Differentiation of Vascular Smooth Muscle Cells into Fibroblast‐Like Cells Contributes to the Stabilization and Repair of Disrupted Atherosclerotic Plaques... by Ming Zhai, Zhijun Lei, Yefei Shi, Jiayun Shi, Yanxi Zeng, Shiyu Gong, Weixia Jian, Jianhui Zhuang, Qing Yu, Mark W. Feinberg, Wenhui Peng

“…Here, an expansion of FSP1+ cells in a tandem stenosis (TS) model of atherosclerotic mice is unveiled, predominantly originating from VSMCs through single‐cell RNA sequencing (scRNA‐seq) analyses and VSMC lineage tracing studies. …”
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Heat shock transcription factor 1 facilitates liver cancer progression by driving super‐enhancer‐mediated transcription of MYCN by Yizhe Liu, Qili Shi, Yue Su, Zhiao Chen, Xianghuo He

“…Additionally, we utilized CUT&Tag, H3K27 acetylation enrichment, and RNA sequencing (RNA‐seq) to investigate the super‐enhancer (SE) regulatory landscapes of HSF1 in liver cancer cell lines. …”
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Curcumin and α/β-Adrenergic Antagonists Cotreatment Reverse Liver Cirrhosis in Hamsters: Participation of Nrf-2 and NF-κB by José Roberto Macías-Pérez, Bruno Jesús Vázquez-López, Martin Humberto Muñoz-Ortega, Liseth Rubí Aldaba-Muruato, Sandra Luz Martínez-Hernández, Esperanza Sánchez-Alemán, Javier Ventura-Juárez

“…In addition, carvedilol treatment and the combination of doxazosin with curcumin increased Nrf-2/NF-κB mRNA ratio and its protein expression in the inflammatory cells in the livers, possibly as another mechanism of hepatoprotection. …”
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Genetic characteristics involving the PD-1/PD-L1/L2 and CD73/A2aR axes and the immunosuppressive microenvironment in DLBCL by Tingting Zhang, Zhenzhen Zhang, Xiubao Ren, Jin He, Zhengzi Qian, Bin Meng, Lei Jiao, Hengqi Liu, Lanfang Li, Lihua Qiu, Shiyong Zhou, Wenchen Gong, Huilai Zhang, Xianhuo Wang

“…The immunosuppressive effect of these two pathways on the tumor microenvironment was evaluated via RNA sequencing. Single-cell RNA sequencing was further applied to investigate the dysfunctional CD8+ T cells. …”
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Co‐Translational Deposition of N6‐Acetyl‐L‐Lysine in Nascent Proteins Contributes to the Acetylome in Mammalian Cells by Dingyuan Guo, Nan Li, Xiaoyan Zhang, Runxin Zhou, Jie He, Xiao‐Ping Ding, Weixing Yu, Fuqiang Tong, Sibi Yin, Yu Wang, Xin Xu, Long Wang, Mingzhu Fan, Shan Feng, Ke Liu, Ke Tang, Zhuqing Ouyang, Yusong R Guo, Yugang Wang

“…Herein, it is reported that Lysyl‐tRNA synthetase (KARS) mediates co‐translational deposition of diet‐derived N6‐acetyl‐L‐lysine (AcK) in nascent proteins to contribute to the acetylome in cells. …”
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