Polarization of ILC2s in Peripheral Blood Might Contribute to Immunosuppressive Microenvironment in Patients with Gastric Cancer by Qingli Bie, Pan Zhang, Zhaoliang Su, Dong Zheng, Xinyu Ying, Yumin Wu, Huijian Yang, Deyu Chen, Shengjun Wang, Huaxi Xu

“…The increased frequency of ILC2s was also found in PBMC of patients by flow cytometry. In addition, the mRNA of Arg1 and iNOS were also significantly increased in patients. …”
Get full text

Dose-dependent role of AMH and AMHR2 signaling in male differentiation and regulation of sex determination in Spotted knifejaw (Oplegnathus punctatus) with X1X1X2X2/X1X2Y chromosom... by Haixia Zhao, Jun Li, Zhizhong Xiao, Yongshuang Xiao

“…Notably, the specific mRNA expression of amhr2 is significantly enriched in amhr2cy, whose extracellular domain exhibits the highest binding affinity for Amh protein, with sexual expression differences manifesting as early as 5 dph. …”
Get full text

Nucleosome patterns in four plant pathogenic fungi with contrasted genome structures by Clairet, Colin, Lapalu, Nicolas, Simon, Adeline, Soyer, Jessica L., Viaud, Muriel, Zehraoui, Enric, Dalmais, Berengère, Fudal, Isabelle, Ponts, Nadia

“…We combined MNase-seq and RNA-seq analyses to concomitantly map nucleosome-rich and transcriptionally active regions during fungal growth in axenic culture; we developed the MNase-seq Tool Suite (MSTS) to analyse and visualise data obtained from MNase-seq experiments in combination with other genomic data and notably RNA-seq expression data. …”
Get full text

Serum Glial Cell Line-Derived Neurotrophic Factor (sGDNF) Is a Novel Biomarker in Predicting Cirrhosis in Patients with Chronic Hepatitis B by Guangyue Yang, Liping Zhuang, Tiantian Sun, Yee Hui Yeo, Le Tao, Wei Zhang, Wenting Ma, Liu Wu, Zongguo Yang, Yanqin Yang, Dongying Xue, Jie Zhang, Rilu Feng, Ebert Matthias P., Steven Dooley, Ekihiro Seki, Ping Liu, Cheng Liu

“…Conclusion. Using serum, tissue mRNA, and biopsy data, our study revealed a significant potential of sGDNF as a novel noninvasive biomarker for cirrhosis in CHB patients.…”
Get full text

Genome- and Transcriptome-Wide Characterization and Expression Analyses of bHLH Transcription Factor Family Reveal Their Relevance to Salt Stress Response in Tomato by Jianling Zhang, Xiaoying Liu, Zuozhen Yin, Tiantian Zhao, Dan Du, Jing Li, Mingku Zhu, Yueying Sun, Yu Pan

“…A total of 30 <i>SlbHLHs</i> were defined as the differentially expressed genes in response to salt stress by RNA-seq. The expression profiles of selected <i>SlbHLHs</i> were varyingly and markedly induced by multiple abiotic stresses and hormone treatments. …”
Get full text

Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST) Gene Delivery Suppresses Activation of Microglia-Like BV-2 Cells by Tongya Yu, Hui Quan, Yuzhen Xu, Yunxiao Dou, Feihong Wang, Yingying Lin, Xue Qi, Yanxin Zhao, Xueyuan Liu

“…When REST was knocked down by a specific short hairpin RNA (sh-RNA), BV-2 cell proliferation, migration, and proinflammation cytokine expression and secretion induced by Aβ1-42 were increased, demonstrating that REST may act as a repressor of microglia-like BV-2 cell activation.…”
Get full text

NIPAL1 as a prognostic biomarker associated with pancreatic adenocarcinoma progression and immune infiltration by Youlong Zhu, Zongze Qi, Shaoqi Zu, Fangchao Yang, Yanming Wang, Lei Zhu, Xintong Li, Ruixue Li, Hong Zhu

“…Therefore, further research is required to determine its diagnostic significance and understand its biological functions in cancer. Primitive RNA sequencing (RNA-seq) data of PAAD from The Cancer Genome Atlas (TCGA) was utilized for bioinformatics analysis to characterize the expression levels of NIPAL1 in tumor and normal tissues. …”
Get full text

Triiodothyronine (T3) increases the expression of the amphiregulin (AREG) oncogene by activating extranuclear pathways in MCF-7 breast cancer cells by Maria Teresa De Sibio, Fernanda Cristina Fontes Moretto, Regiane Marques Castro Olimpio, Miriane de Oliveira, Lucas Solla Mathias, Vinícius Vigliazzi Peghinelli, Helena Paim Tilli, Bianca Mariani Gonçalves, Dariane Beatriz Marino Cardoso, Larissa Silva Dall Aqua, Igor de Carvalho Depra, Mariana Menezes Lourenço, Aline Carbonera Luvizon, Paula de Oliveira Montandon Hokama, Maria Tereza Nunes, Marna Eliana Sakalem, Célia Regina Nogueira

“…A control group (C) received no T3 or inhibitors. Analyses of mRNA and protein expression were done using RT-qPCR and Western blot, respectively. …”
Get full text

Machine learning-driven identification of critical gene programs and key transcription factors in migraine by Lei Zhang, Yujie Li, Yunhao Xu, Wei Wang, Guangyu Guo

“…Methods We utilized single-nucleus RNA sequencing (snRNA-seq) data from 43 brain regions, along with genome-wide association study (GWAS) data, to investigate susceptibility to migraine. …”
Get full text

Revolutionizing ESCC prognosis: the efficiency of tumor-infiltrating immune cells (TIIC) signature score by Haixia Wang, Shaowei Ma, Zixin Yang, Ren Niu, Haiyong Zhu, Shujun Li, Shaolin Gao, Zhirong Li, Yanhua Tian

“…Methods Transcriptome data and scRNA-seq data of ESCC samples were extracted from the GEO and TCGA databases. …”
Get full text

Potential Strategies Applied by <i>Metschnikowia bicuspidata</i> to Survive the Immunity of Its Crustacean Hosts by Ji Zhang, Bingyu Li, Bingnan Zuo, Xiaodong Li

“…It is shown that the expression trends of these tested DEGs in qRT-PCR assays are the same as those in RNA-Seq assays. This study not only provides insights into strategies facilitating <i>M. bicuspidata</i>’s survival within <i>E. sinensis</i>, initially elucidating the pathogenesis of this yeast, but also recommends a useful molecular tool regarding qRT-PCR assays in this pathogen.…”
Get full text

Dietary γ-Aminobutyric Acid Promotes Growth and Immune System Performance and Improves Erythropoiesis and Angiogenesis in Gibel Carp (<i>Carassius auratus gibelio</i>) by Xinlan Bai, Lu Zhang, Hualiang Liang, Dongyu Huang, Mingchun Ren, Haifeng Mi

“…In terms of oxygen-carrying capacity, the mRNA expression of <i>epo</i>, <i>tf</i>, <i>tfr1</i>, <i>ho-1</i>, and <i>vegf</i> was markedly increased at the level of 449 mg/kg (G2). …”
Get full text

TSP-1-1223 A/G Polymorphism as a Potential Predictor of the Recurrence Risk of Bladder Cancer in a Chinese Population by Xiao Yang, Pengchao Li, Xuejian Yang, Chao Qin, Qiang Cao, Zhengdong Zhang, Meilin Wang, Hongzhou Cai, Jinbao Gu, Jun Tao, Min Gu, Qiang Lu, Changjun Yin

“…The expression of TSP-1 mRNA in bladder cancer tissues was lower in patients with an AG genotype than in those with an AA genotype. …”
Get full text

Integrative proteomic-transcriptomic analysis revealed the lifestyles of Lactobacillus paracasei H4-11 and Kluyveromyces marxianus L1-1 under co-cultivation conditions by Na Liu, Likang Qin, Haiying Zeng, Anyan Wen, Song Miao

“…The combined analysis results of RNA sequencing (RNA-seq) technology and 4D label-free quantitative (4D LFQ) proteomics explained the flavor formation pathways in rice-acid soup inoculated with L. paracasei H4-11 and K. marxianus L1-1. …”
Get full text

Direct and Indirect Protein Interactions Link FUS Aggregation to Histone Post-Translational Modification Dysregulation and Growth Suppression in an ALS/FTD Yeast Model by Seth A. Bennett, Samantha N. Cobos, Raven M. A. Fisher, Elizaveta Son, Rania Frederic, Rianna Segal, Huda Yousuf, Kaitlyn Chan, David K. Dansu, Mariana P. Torrente

“…Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are incurable neurodegenerative disorders sharing pathological and genetic features, including mutations in the <i>FUS</i> gene. FUS is an RNA-binding protein that mislocalizes to the cytoplasm and aggregates in ALS/FTD. …”
Get full text

Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis by Ruyu Shi, Xing Li, Bing Gao, Chunhong Zhang, Minhui Li

“…INS-1 cells were treated with C. daurica, cell viability assay, RNA-seq technology, superoxide dismutase activity and malonaldehyde content, quantitative real time-PCR and Western blot analysis were used to study the protective effects of C. daurica. …”
Get full text

Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation by Yanqin Wang, Xinxin Li, Xiaoyun Xing, Haibo Xue, Ruiqun Qi, Hong Ji, Lei Ma

“…Quantitative real-time RT-PCR detected Hes1 mRNA expression. Enzyme-linked immunosorbent assay and western blot measured IL-17A serum concentration and protein expression. …”
Get full text

A 7-Amino Acid Peptide Mimic from Hepatitis C Virus Hypervariable Region 1 Inhibits Mouse Lung Th9 Cell Differentiation by Blocking CD81 Signaling during Allergic Lung Inflammation by Wanzhou Zhao, Conghao Tan, Xi Yu, Ruihe Yu, Qibing Mei, Yun Cheng

“…Furthermore, 7P inhibited the differentiation of human Th9 cells with high CD81 expression from naïve CD4+ T cells by blocking CD81 signaling. CD81 siRNA significantly reduced Th9 cell differentiation from naïve CD4+ T cells in vitro. …”
Get full text

Exosomal non-coding RNAs in the regulation of bone metabolism homeostasis: Molecular mechanism and therapeutic potential by Chengxiong Huang, Yu Xiao, Liming Qing, Juyu Tang, Panfeng Wu

“…In this review, we focus on the regulation mechanism and function of bone homeostasis mediated by exosomal ncRNAs (Exos-ncRNAs), including macrophage polarization, autophagy, angiogenesis, signal transduction and competing endogenous RNA (ceRNA). We summarized the therapeutic strategies and potential drugs of Exos-ncRNAs in metabolic bone disease. …”
Get full text

Incidence of methicillin, inducible clindamycin, and vancomycin resistance in Staphylococcus aureus clinical isolates in Gombe, Northeastern Nigeria by Haruna Adamu, Celestina T. Otegwu, Fatima B. Abdullahi, Babajide A. Tytler, Busayo O. Olayinka

“…The species-specific 16S rRNA gene was investigated in the presumptive identified S. aureus isolates whereas the mecA and vanA genes were investigated in cefoxitin and vancomycin resistant S. aureus isolates via PCR. …”
Get full text