Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in Osteoarthritis by Lingxiao Xu, Qiuyue Peng, Wenhua Xuan, Xiaoke Feng, Xiangqing Kong, Miaojia Zhang, Wenfeng Tan, Meilang Xue, Fang Wang

“…Furthermore, recombinant IL-29 augmented the mRNA expression of IL-1β, IL-6, IL-8, and matrix-metalloproteinase-3 (MMP-3) in OA FLS and increased cartilage degradation when ex vivo OA cartilage explant was coincubated with OA FLS. …”
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ISU201 Enhances the Resolution of Airway Inflammation in a Mouse Model of an Acute Exacerbation of Asthma by Yuka Hiroshima, Linda Garthwaite, Kenneth Hsu, Hyouna Yoo, Sang-Ho Park, Carolyn L. Geczy, Rakesh K. Kumar, Cristan Herbert

“…ISU201 was administered to mice 2 and 8 hours later, while pulmonary inflammation and expression of mRNA for chemokines and proinflammatory cytokines were assessed after 4, 12, and 24 hours. …”
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Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer by Asmaa M. Elfiky, May M. Eid, May El-Manawaty, Zeinab A. Elshahid, Elham Mohamed Youssef, Khaled Mahmoud

“…This study aims to create new super paramagnetic iron oxide nano-carrier (SPION) that can effectively transport miRNA to specific CRC cell lines. In addition, evaluate the efficiency of this nano-formulation as a therapeutic candidate for CRC. …”
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Distinct Redox Signalling following Macrophage Activation Influences Profibrotic Activity by Caitlin V. Lewis, Antony Vinh, Henry Diep, Chrishan S. Samuel, Grant R. Drummond, Barbara K. Kemp-Harper

“…Human primary and THP-1 macrophages were treated with IFN-γ+LPS or IL-4-activating stimuli, and mRNA expression of established M1 (CXCL11, CCR7, IL-1β) and M2 (MRC-1, CCL18, CCL22) markers was used to confirm activation. …”
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NF-κB-94ins/del ATTG Genotype Contributes to the Susceptibility and Imbalanced Th17 Cells in Patients with Immune Thrombocytopenia by Jie Yu, Mingqiang Hua, Xueyun Zhao, Rui Wang, Chaoqing Zhong, Chen Zhang, Ruiqing Wang, Guosheng Li, Na He, Ming Hou, Daoxin Ma

“…Compared with controls, NF-κB gene expression was significantly decreased and WW or WD genotype ITP patients displayed higher mRNA expression than DD individuals. Similarly, the mRNA expression of NLRP3 was also increased in the WW genotype. …”
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Antitumor Effect of Demethylzeylasteral (T-96) on Triple-Negative Breast Cancer via LSD1-Mediate Epigenetic Mechanisms by Zhengjie Shen, Yongjuan Gu, Ruiyang Jiang, Heya Qian, Siyuan Li, Lixian Xu, Wenzhe Gu, Yun Zuo

“…Knockdown of LSD1 by siRNA blocked the pharmacological activity of T-96. …”
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Pathogenic Role of Iron Deposition in Reticuloendothelial Cells during the Development of Chronic Hepatitis C by Hironori Mitsuyoshi, Kohichiroh Yasui, Kanji Yamaguchi, Masahito Minami, Takeshi Okanoue, Yoshito Itoh

“…The levels of serum soluble TNF-α receptor (sTNFR2) and hepatic hepcidin mRNA and the efficacy of phlebotomy were compared among patients with different iron deposition patterns. …”
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Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathway by Kaifang Guan, Xiaolin Liu, Weihong Lu, Yuhao Mao, Yirong Mao, Ying Ma, Rongchu Wang, Qiming Li

“…Flow cytometry and quantitative real-time PCR proved that MFG-E8 promoted cell proliferation by upregulating mRNA expression of cyclin D1, cyclin E1, CDK, and downregulating mRNA expression of p21 and p27, thereby increasing the S and G2/M phase and decreasing the G0/G1 phase. …”
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circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8 by Xueliang Yang, Quan Sun, Yongming Song, Wenli Li

“…The quantitative real-time polymerase chain reaction (RT-qPCR) and western blot analyses were used for examining circHUWE1, microRNA-34a-5p (miR-34a-5p), and tumor necrosis factor alpha-induced protein 8 (TNFAIP8). …”
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Targeted Inhibition of the miR-199a/214 Cluster by CRISPR Interference Augments the Tumor Tropism of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells under Hypoxic Con... by Yumei Luo, Xuehu Xu, Xiuli An, Xiaofang Sun, Shu Wang, Detu Zhu

“…We first developed a baculovirus-delivered CRISPR interference (CRISPRi) system that sterically blocked the E-box element in the promoter of the miR-199a/214 cluster with an RNA-guided catalytically dead Cas9 (dCas9). We then applied this CRISPRi system to hiPS-NSCs and successfully suppressed the expression of miR-199a-5p, miR-199a-3p, and miR-214 in the microRNA gene cluster. …”
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The Anti-Inflammatory Effects of Shinbaro3 Is Mediated by Downregulation of the TLR4 Signalling Pathway in LPS-Stimulated RAW 264.7 Macrophages by Hwa-Jin Chung, Wonil Koh, Won Kyung Kim, Joon-Shik Shin, Jinho Lee, Sang Kook Lee, In-Hyuk Ha

“…Shinbaro3 also downregulated the mRNA and protein expression of inflammatory mediators in a dose-dependent manner. …”
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Allelic variation in an expansin, MdEXP-A1, contributes to flesh firmness at harvest in apples by Qiufang Su, Yifeng Feng, Xianglu Li, Zidun Wang, Yuanwen Zhong, Zhengyang Zhao, Huijuan Yang

“…Quantitative trait loci (QTLs) were analyzed through bulked segregant analysis sequence (BSA-seq) and comparative transcriptome analysis (RNA-seq) to explore the genetic basis of firmness formation. …”
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miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8 by Zhongsi Hong, Haiyu Hong, Jian Liu, Xiaobin Zheng, Mingxing Huang, Chunna Li, Jinyu Xia

“…Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects. …”
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Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study by Birgit Henrich, Ilka Hermann, Mara Di Giulio, Karl Köhrer, René Deenen, Sugi Sivalingam, Ulrike Peters, Thomas Beikler, Ralf Janda, Stefan Rüttermann

“…In situ: TaqMan PCR and 16S rRNA Next Generation Sequencing (NGS) were performed. …”
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Knockdown of Fibromodulin Inhibits Proliferation and Migration of RPE Cell via the VEGFR2-AKT Pathway by He Hu, Shanshan Li, Jianqiao Li, Chao Huang, Fang Zhou, Li Zhao, Wenzhen Yu, Xiao Qin

“…Small interfering (si) RNA-based gene transfer technology was used to decrease FMOD expression and to study its effects on RPEs in vitro. …”
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Correlation of susceptibility to ortho-aminoazotolueneinduced hepatocarcinogenesis with Car and Ahr signaling pathway activation in mice by N. V. Baginskaya, E. V. Kashina, M. Yu. Shamanina, S. I. Ilnitskaya, V. I. Kaledin, V. A. Mordvinov

“…In this study, we investigated the effect of chronic OAT administration on the mRNA expression levels of Ahr, Car and their target genes Cyp1a1 and Cyp2b10 in the liver of DD/He (DD) and CC57BR/Mv (BR) mouse strains contrasting in sensitivity to hepatocarcinogenesis. …”
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Unlocking the multifaceted roles of GLP-1: Physiological functions and therapeutic potential by Tohada M. AL-Noshokaty, Rehab Abdelhamid, Nourhan M. Abdelmaksoud, Aya Khaled, Mariam Hossam, Razan Ahmed, Toka Saber, Shahd Khaled, Shereen Saeid Elshaer, Ahmed I. Abulsoud

“…Also, microribonucleic acid (RNA) (miRNAs), critical regulators of gene expression, are introduced as potential modulators of GLP-1 signaling. …”
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Maternal obesity exacerbates the responsiveness of offspring BALB/c mice to cow’s milk protein-induced food allergy by Jingxin Gao, Tiange Li, Dong Liang, Han Gong, Liang Zhao, Xueying Mao

“…Furthermore, maternal obesity led to a lower level of occludin mRNA expression in BLG -sensitized mice. The mice born to obese mothers exhibited increased mRNA expression levels of GATA-3, interleukin (IL)-4 and IL-10 in jejunum after BLG sensitization, indicating maternal obesity intensified Th2-type biased immune responses. …”
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Oral Cancer Stem Cell-Derived Small Extracellular Vesicles Promote M2 Macrophage Polarization and Suppress CD4+ T-Cell Activity by Transferring UCA1 and Targeting LAMC2 by Lan Wu, Sai Ye, Yilin Yao, Chenping Zhang, Wei Liu

“…Mechanistically, we revealed that the lncRNA UCA1, by binding to miR-134, modulated the PI3K/AKT pathway in macrophages via targeting LAMC2. …”
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Monoallelic variants resulting in substitutions of MAB21L1 Arg51 Cause Aniridia and microphthalmia. by Hildegard Nikki Hall, Hemant Bengani, Robert B Hufnagel, Giuseppe Damante, Morad Ansari, Joseph A Marsh, Graeme R Grimes, Alex von Kriegsheim, David Moore, Lisa McKie, Jamalia Rahmat, Catia Mio, Moira Blyth, Wee Teik Keng, Lily Islam, Meriel McEntargart, Marcel M Mannens, Veronica Van Heyningen, Joe Rainger, Brian P Brooks, David R FitzPatrick

“…We found limited evidence for mutation-specific interactions with MSI2/Musashi-2, an RNA-binding proteins with effects on many different developmental pathways. …”
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