First detection and phylogenetic analysis of Trypanosoma species in European wolves and bears: discovery of novel haplotypes by Blanka Orłowska, Magdalena Świsłocka-Cutter, Katarzyna Filip-Hutsch, Daniel Młocicki, Adam Olszewski, Marek Asman, Krzysztof Anusz, Joanna Werszko

“…The diversity and occurrence of Trypanosoma species among wildlife in Europe were investigated through the analysis of 18S rRNA gene sequences. The phylogenetic analysis showed three new haplotypes of the 18S rRNA gene of Trypanosoma sp. isolated from two grey wolves (Canis lupus) and two brown bears (Ursus arctos). …”
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Acute hepatitis of non-specified etiology by Ye. V. Tsyganova, O. O. Znoyko, N. D. Yushchuk, K. R. Dudina, M. G. Isagulyants, N. V. Petrakova, T. V. Petrova, M. I. Mikhaylov

“…To estimate pathogenic and diagnostic value of detection of hepatotropic viruses components (DNA, RNA, antigens) and antiviral immune response at acute hepatitis of non-specified etiology by the methods of enzyme-linked immunoassay (EIA), polymerase chain reaction (PCR) and Т-cellular lymphocytes proliferation in response to HCV antigens stimulation.Methods. …”
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Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samples by Yun-Shan Li, Koichi Fujisawa, Kazuaki Kawai

“…Abstract Background Urinary 8-hydroxyguanosine (8-OHGuo) levels serve as a biomarker for oxidative stress and hydroxyl radical–induced RNA damage. Evaluating the diurnal and daily fluctuations in urinary 8-OHGuo excretion levels is essential for understanding its implications. …”
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MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways by Minmin Cai, Liufang Sheng

“…The cell viability and apoptosis affected by rhMFG-E8 were tested by MTT and flow cytometry analysis, respectively. Then, the mRNA expression of MFG-E8 was detected by qRT-PCR. …”
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Contribution of OqxAB Efflux Pump in Selection of Fluoroquinolone-Resistant Klebsiella pneumoniae by Orsolya Szabo, Bela Kocsis, Nikolett Szabo, Katalin Kristof, Dora Szabo

“…Quantitative reverse-transcriptase PCR was performed from each RNA sample. Mutation in gyrA and parC genes was analyzed in each strain and multilocus sequence typing (MLST) was performed. …”
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Characterizing temporal and global host innate immune responses against SARS-CoV-1 and -2 infection in pathologically relevant human lung epithelial cells. by Vivian Y Tat, Aleksandra K Drelich, Pinghan Huang, Kamil Khanipov, Jason C Hsu, Steven G Widen, Chien-Te Kent Tseng, George Golovko

“…In this study, our objective is to use a systems biology approach to explore the magnitude and scope of innate immune responses triggered by SARS-CoV-1 and -2 infection over time in pathologically relevant human lung epithelial cells (Calu-3/2B4 cells). Total RNA extracted at 12, 24, and 48 hours after β-CoVs or mock infection of Calu-3/2B4 cells were subjected to RNA sequencing and functional enrichment analysis to select genes whose expressions were significantly modulated post-infection. …”
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NAT10 promotes ovarian cancer cell migration, invasion, and stemness via N4-acetylcytidine modification of CAPRIN1 by Yang Song, Min Cheng

“…The molecular mechanism of NAT10 function was analyzed using bioinformatics, ac4C- RNA immunoprecipitation, and actinomycin D treatment assay. …”
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Sparse Gene Coexpression Network Analysis Reveals EIF3J-AS1 as a Prognostic Marker for Breast Cancer by Xin Chen, Zuyuan Yang, Chao Yang, Kan Xie, Weijun Sun, Shengli Xie

“…Kaplan–Meier survival analysis revealed that EIF3J-AS1, a downregulated lncRNA in breast tumor, is a potential prognostic marker for breast cancer. …”
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Anticolitic Effect of Berberine in Rat Experimental Model: Impact of PGE2/p38 MAPK Pathways by Li Jia, Kuijin Xue, Junheng Liu, Ola A. Habotta, Lianhai Hu, Ahmed E. Abdel Moneim

“…Notable downregulation of mRNA expression of p38 MAPK and increased protein expression of TGF-β were achieved by BER treatment. …”
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Single-Cell Analyses Reveal Necroptosis’s Potential Role in Neuron Degeneration and Show Enhanced Neuron-Immune Cell Interaction in Parkinson’s Disease Progression by Xiaomei Zeng, Zhifen Han, Kehan Chen, Peng Zeng, Yidan Tang, Lijuan Li

“…Although widely investigated on its pathology and treatment, the disease remains incompletely understood. Single-cell RNA sequencing (scRNA-seq) has been applied to the area of PD, providing valuable data for related research. …”
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Differences in gene expression profile between vocal cord Leukoplakia and normal larynx mucosa by gene chip by Jianhua Peng, He Li, Jun Chen, Xianming Wu, Tao Jiang, Xiaoyun Chen

“…Furthermore, we identified an antisense lncRNA (LOC100506801), an enhancer-like lncRNA (AK057351) and three long intergenetic noncoding RNAs including XLOC_008001, XLOC_011989 and XLOC_007341. …”
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The Coincidence of Newly Diagnosed Type 1 Diabetes Mellitus with IgM Antibody Positivity to Enteroviruses and Respiratory Tract Viruses by Murat Karaoglan, Fahriye Eksi

“…The IgM antibodies against enteroviruses and respiratory viruses were studied using the indirect immunofluorescence assay (IFA) test, and no CBV4-specific RNA was detected in the children. The onset times of T1DM were classified into fall-winter and spring-summer seasons and separated into cold, moderate, or warm months in terms of temperature. …”
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Inhibition of Cathepsin S Produces Neuroprotective Effects after Traumatic Brain Injury in Mice by Jianguo Xu, Handong Wang, Ke Ding, Xinyu Lu, Tao Li, Jiawei Wang, Chunxi Wang, Jian Wang

“…In this study, we investigated the time course of CatS protein and mRNA expression and the cellular distribution of CatS in a mouse model of traumatic brain injury (TBI). …”
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New Insights into Behçet’s Syndrome Metabolic Reprogramming: Citrate Pathway Dysregulation by Anna Santarsiero, Pietro Leccese, Paolo Convertini, Angela Padula, Paolo Abriola, Salvatore D’Angelo, Faustino Bisaccia, Vittoria Infantino

“…Real-time PCR experiments were performed on PBMCs to quantify SLC25A1 and ACLY mRNA levels. Data processing through the Kruskal-Wallis test and Dunn’s multiple comparison test as post hoc showed higher SLC25A1 and ACLY mRNA levels in BS patients compared to those in healthy controls. …”
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Characterization and phylogenetic analysis of the chloroplast genome of endangered Impatiens chishuiensis by LAN Wenxiang, MO Qing, HUANG Haiquan, HUANG Meijuan

“…It encodes a total of 120 genes, including 95 protein-coding genes, 8 rRNA genes, and 17 tRNA genes. (2) A total of 87 SSR sequences was detected. 20 327 codons were detected, with leucine (Leu) being the most abundant and tryptophan (Trp) being the least. (3) Impatiens was divided into two subgenera, including subg. …”
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Resistin Regulates Pituitary Lipid Metabolism and Inflammation In Vivo and In Vitro by F. Rodriguez-Pacheco, M. G. Novelle, M. J. Vazquez, E. Garcia-Escobar, F. Soriguer, G. Rojo-Martinez, E. García-Fuentes, M. M. Malagon, C. Dieguez

“…Here, by a combination of in vivo and in vitro experimental models, we demonstrated that central acute and chronic administration of resistin enhance mRNA levels of the lipid metabolic enzymes which participated on lipolysis and moreover inhibiting mRNA levels of the lipid metabolic enzymes involved in lipogenesis. …”
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The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats by Ying Yu, Xian-Jie Jia, Wei-ping Zhang, Ting-ting Fang, Jie Hu, Shan-Feng Ma, Qin Gao

“…Morphology of collagen in myocardial tissue was observed with Masson’s trichrome staining method and collagen volume fraction (CVF) was analysed. The mRNA expression of ALDH2 was assessed with Real-Time PCR. …”
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Bone Marrow Mesenchymal Stem Cells Decrease the Expression of RANKL in Collagen-Induced Arthritis Rats via Reducing the Levels of IL-22 by Fang Li, Xin Li, Guiyan Liu, Chong Gao, Xiaofeng Li

“…In addition, the expression of RANKL mRNA was measured by RT-PCR in the fibroblast-like synoviocytes (FLSs), cultured with IL-22 in vitro, which were delivered from the joints of CIA rats treated with or without MSCs. …”
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Keystone virus: a mosquito-borne arbovirus by Abdullah A. Alomar, Lawrence E. Reeves, Barry W. Alto

“… Keystone virus (KEYV) is an emerging negative-sense single-stranded RNA arthropod-borne virus (arbovirus) that can be transmitted to humans by the bite of an infected mosquito vector. …”
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REPETITIVE DNA SEQUENCES IN THE GENOMES OF PLANTS by A. B. Shcherban

“…The main classes of repetitive DNA sequences, including coding (rRNA genes) and non-coding sequences (tandem and dispersed repeats) are characterized. …”
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