Network pharmacology combined with experimental verification for exploring the potential mechanism of phellodendrine against depression by Lili Hu, Na Wu, Jue Wang, Donghui Cai

“…PHE affected the levels of IL6 and IL1β with different concentrations. The mRNA levels of CHRM1, HTR1A and key targets of the PI3K/Akt signalling pathway were also influenced. …”
Get full text

The Role of Epoxyeicosatrienoic Acids in Diabetes Mellitus-Induced Impaired Vascular Relaxation of Aortic Rings in Ovariectomized Sprague-Dawley Rats by Dragan Manojlović, Ana Stupin, Anita Matić, Zrinka Mihaljević, Sanja Novak, Ines Drenjančević

“…MS-PPOH did not reduce AChIR in OVX-DM rats as it did in OVX-CTR ones. CYP4a3 mRNA expression in OVX-DM rats was significantly lower compared to that in the OVX-CTR group. …”
Get full text

Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms by Hongyu Xu, Zetian Jia, Junhui Liu, Runming Liu, Wei Wei, Xiang Li

“…We describe steps for sample collection, fecal genomic DNA extraction, rRNA PCR amplification, sequencing library construction, and rRNA sequencing. …”
Get full text

Endoplasmic Reticulum Stress Regulates Scleral Remodeling in a Guinea Pig Model of Form-Deprivation Myopia by Chengcheng Zhu, Qingzhong Chen, Ying Yuan, Min Li, Bilian Ke

“…CRT was knocked down by lentivirus-mediated CRT shRNA transfection. The expression levels of GRP78, CHOP, TGF-β1, and COL1A1 were analyzed by qRT-PCR or western blotting. …”
Get full text

Label-free proteomic analysis of Duchenne and Becker muscular dystrophy showed decreased sarcomere proteins and increased ubiquitination-related proteins by Juliana Cristina Tobar da Silva, Mariângela Rangel Alves Nogueira, Yara Martins da Silva, Fábio César Sousa Nogueira, Nathalie Henriques Silva Canedo, Katia Carneiro, Denise de Abreu Pereira

“…Enrichment of functions associated with cell structure and RNA binding was also observed, including cytoskeletal protein binding and RNA binding. …”
Get full text

Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes by Julia Platas, Maria Isabel Guillén, María Dolores Pérez del Caz, Francisco Gomar, Vicente Mirabet, Maria José Alcaraz

“…CM enhanced type II collagen expression in OA chondrocytes while decreasing matrix metalloproteinase (MMP) activity in cell supernatants as well as the levels of MMP-3 and MMP-13 proteins and mRNA in OA chondrocytes stimulated with interleukin- (IL-) 1β. …”
Get full text

Proteogenomic Analysis Reveals Proteins Involved in the First Step of Adipogenesis in Human Adipose-Derived Stem Cells by Bernardo Bonilauri, Amanda C. Camillo-Andrade, Marlon D. M. Santos, Juliana de S. da G. Fischer, Paulo C. Carvalho, Bruno Dallagiovanna

“…We also used our previous next-generation sequencing data (RNA-seq) of the total and polysomal mRNA fractions of hASC to study posttranscriptional regulation during the initial steps of adipogenesis. …”
Get full text

Downregulation of Thromboxane A2 Receptor Occurs Mainly via Nuclear Factor-KappaB Signaling Pathway in Rat Renal Artery by Yaping Zhang, Man Mi, Yan-Hua Xie, Si-Wang Wang, Lars Edvinsson, Cang-Bao Xu

“…Downregulation of TP receptors was monitored using myograph (contractile function), real-time PCR (receptor mRNA), and immunohistochemistry (receptor protein). …”
Get full text

Exploring the Molecular Composition of Dissolved Organic Matter and Its Connection to Microbial Communities in Industrial-Scale Anaerobic Digestion of Chicken Manure by Juan Hu, Yurui Zeng, Aibin Hu, Xiaofeng Wang

“…In addition, the 16S rRNA results revealed microorganisms that were centered on metabolic function in the production of volatile fatty acids, H₂S/CH₄, and the hydrolysis reaction in the AD process. …”
Get full text

Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4 by Patricia Méndez-Samperio, Elena Miranda, Abraham Vázquez

“…We found that, although M. bovis BCG induced gene expression of CXCL-8 and CXCL-10 in M. bovis BCG-infected human epithelial cells, CXCL-8 mRNA level was significantly reduced by IL-4, whereas no significant effect of IL-4 was observed on CXCL10 mRNA level. …”
Get full text

Methylation Status of Vitamin D Receptor Gene Promoter in Benign and Malignant Adrenal Tumors by Catia Pilon, Andrea Rebellato, Riccardo Urbanet, Vincenza Guzzardo, Rocco Cappellesso, Hironobu Sasano, Ambrogio Fassina, Francesco Fallo

“…We previously showed a decreased expression of vitamin D receptor (VDR) mRNA/protein in a small group of adrenocortical carcinoma (ACC) tissues, suggesting the loss of a protective role of VDR against malignant cell growth in this cancer type. …”
Get full text

Identification of Potential Abnormal Methylation-Modified Genes in Coronary Artery Ectasia by Xiuchun Yang, Yijun Zong, Zhentian Zhang, Yan Zhao, Xueying Gao, Jie Zhang, Qian Hou, Renyi Li, Bing Xiao

“…A total of 152 DEGs and 4318 DMGs were obtained from RNA sequencing and the GSE87016 dataset, respectively. …”
Get full text

Molecular Evidence of Emerged Pulmonary Lophomoniasis due to Lophomonas blattarum among Hospitalized Patients in Southwestern Iran: A National Registry-Based Study by Kobra Mokhtarian, Simin Taghipour, Maryam Nakhaei, Amirmasoud Taheri, Ali Sharifpour, Mahdi Fakhar, Hajar Ziaei Hezarjaribi

“…Samples are referred to the Iranian National Registry Center for Lophomoniasis (INRCL), Mazandaran Province, Northern Iran, for detecting Lophomonas spp. infection by a conventionally small subunit ribosomal RNA (SSU rRNA) PCR test. Results. A total of 132 frozen BALF specimens were examined, 36 (27.3%) tested Lophomonas spp. positive using the conventional PCR technique. …”
Get full text

Multi-omics analysis reveals that neutrophil extracellular traps related gene TIMP1 promotes CRC progression and influences ferroptosis by Yuzhao Jin, Luyu Liao, Qianping Chen, Bufu Tang, Jin Jiang, Ji Zhu, Minghua Bai

“…Method We jointly screened three major NETs genes through machine learning. Large-sample RNA transcriptome and single-cell transcriptome analysis further confirmed that TIMP1 is a core gene in NETs. …”
Get full text

GOLPH3 inhibits glioma cell apoptosis through the JNK signaling pathway by Shao Xie, Shao Xie, Jiahai Ding, Zhaohao Wang, Hengliang Shi, Zheng-Quan Yu

“…However, its precise role in glioma progression remains under active investigation.MethodsTo elucidate the function of GOLPH3, U87 glioma cells were transfected with GOLPH3-specific small interfering RNA (siRNA) to suppress its expression. An in vivo glioma model was generated by implanting GOLPH3-knockdown U87 cells into nude mice. …”
Get full text

CRISPR/Cas9, a universal tool for genomic engineering by A. V. Smirnov, A. M. Yunusova, V. A. Lukyanchikova, N. R. Battulin

“…The CRISPR/Cas9 system was initially described as an element of archeal and bacterial immunity, but gained much attention recently for its outstanding ability to be programmed to target any genomic loci through a short 20-nucleotide sgRNA region. Here we review some modern applications of the CRISPR/Cas9 system. …”
Get full text

Global Transcriptomic Analysis of Inbred Lines Reveal Candidate Genes for Response to Maize Lethal Necrosis by Ann Murithi, Gayathri Panangipalli, Zhengyu Wen, Michael S. Olsen, Thomas Lübberstedt, Kanwarpal S. Dhugga, Mark Jung

“…This study analyzed the transcriptomes of four commercially used maize inbred lines and a non-adapted inbred line, all with varying response levels to MLN. RNA-Seq revealed differentially expressed genes in response to infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV), the causative agents of MLN. …”
Get full text

Identification of KRT16 and ANXA10 as cell cycle regulation genes for lung adenocarcinoma based on self-transcriptome sequencing of surgical samples and TCGA public data mining by Wen-jian Liu, Jia-pan Shen, Ren-quan Zhang, Xiao-yun Fan

“…The expression was validated at mRNA and protein levels, and the gene knockdown was used to examine the impact and underlying mechanisms on lung cancer cells. …”
Get full text

Golgin Subfamily A Member 5 Is Essential for Production of Extracellular Matrix Proteins during TGF-β1-Induced Periodontal Ligament-Fibroblastic Differentiation by Hyun-Jin Kim, Seong-Min Kim, Min-Jeong Choi, Young-Joo Jang

“…When the TGF-β1 signaling pathway was blocked or GOLGA5 was depleted by siRNA, the levels of extracellular matrix (ECM) proteins, such as type I collagen and fibronectin, decreased in PDL-fibroblastic progenitors. …”
Get full text

Increased nuclear expression of DNA damage inducible transcript 4 can serve as a potential prognostic biomarker in patients with gliomas: a study based on data mining and experimen... by Alireza Sadeghipour, Fahimeh Fattahi, Zahra Madjd, Fatemeh Tajik, Farnoosh Sedaghati, Leili Saeednejad Zanjani

“…Materials and methods The expression of DDIT4 at the mRNA level and its potential role as a prognostic biomarker in gliomas were analyzed using the GEPIA tool. …”
Get full text