An Overview of Long Noncoding RNAs Involved in Bone Regeneration from Mesenchymal Stem Cells by Shuping Peng, Lihua Cao, Shiwei He, Yancheng Zhong, Haotian Ma, Yanru Zhang, Cijun Shuai

“…In this review, we will summarize the roles and molecular mechanism of lncRNAs including H19, MALAT1, MODR, HOTAIR, DANCR, MEG3, HoxA-AS3, and MIAT in osteogenesis ossification; lncRNA ZBED3-AS1 and CTA-941F9.9, DANCR, and HIT in chondrogenic differentiation; and lncRNA DANCR in osteoclast differentiation. …”
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TGF-β signaling orchestrates cancer-associated fibroblasts in the tumor microenvironment of human hepatocellular carcinoma: unveiling insights and clinical significance by Junwei Ge, Hongwei Jiang, Junjun Chen, Xuemin Chen, Yue Zhang, Liangrong Shi, Xiao Zheng, Jingting Jiang, Lujun Chen

“…In our recent study, drawing from the GEO database, we identified two fibroblast subtypes in HCC through single-cell RNA sequencing (scRNA-seq) and explore the expression and distribution of TGF-β and its receptors in the TME of HCC. …”
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Molecular genetics of idiopathic pulmonary fibrosis by R. N. Mustafin

“…Therefore, dysregulation of transposons, which serve as key sources of noncoding RNA and affect mechanisms of aging, may serve as a driver for IPF development. …”
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Transient degradation of NF-κB proteins in macrophages after interaction with mast cell granules by Noriko Ito, Yuai Li, Tsuneo Suzuki, Daniel J. Stechschulte, Kottarappat N. Dileepan

“…On the other hand, in peritoneal macrophages, MCG induce more extensive modifications to NF-κB proteins with associated inhibition of iNOS or TNF-α mRNA expression.…”
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Functional analysis of a novel homozygous missense IVD gene variant: a case report with dual genetic diagnoses by Yuying Zhu, Ke Wu, Hanying Wen

“…The assays of relative IVD gene mRNA expression, IVD protein expression, and enzymatic activity were used.ResultsWhole-exome sequencing identified a novel homozygous missense variant in the IVD gene (NM_002225.5) c.1006T>C (p.Cys336Arg) within a region of homozygosity of 15q11.2-q21.3. …”
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Repression of the stress granule protein G3BP2 inhibits immune checkpoint molecule PD‐L1 by Yanhong Zhang, Changli Yue, Anna M. Krichevsky, Igor Garkavtsev

“…Cancer cells treated with C108 or cancer cells with genetically repressed G3BP2 had decreased PD‐L1 expression due to enhanced mRNA degradation. Our study provides a compelling mechanism linking stress granule formation and immune checkpoint program of cancer, suggesting this link may provide new opportunities for improving anticancer immunotherapy.…”
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Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia by Faruku Bande, Siti Suri Arshad, Latiffah Hassan, Zunita Zakaria

“…A nested PCR assay was used to determine the viral RNA and proviral DNA status of naturally infected cats. …”
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The Human Microglia Atlas (HuMicA) unravels changes in disease-associated microglia subsets across neurodegenerative conditions by Ricardo Martins-Ferreira, Josep Calafell-Segura, Bárbara Leal, Javier Rodríguez-Ubreva, Elena Martínez-Saez, Elisabetta Mereu, Paulo Pinho E Costa, Ariadna Laguna, Esteban Ballestar

“…We constructed an atlas of human brain immune cells by integrating nineteen single-nucleus RNA-seq and single-cell RNA-seq datasets from multiple neurodegenerative conditions, comprising 241 samples from patients with Alzheimer’s disease, autism spectrum disorder, epilepsy, multiple sclerosis, Lewy body diseases, COVID-19, and healthy controls. …”
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Modeling the endocrine control of vitellogenin production in female rainbow trout by Kaitlin Sundling, Gheorghe Craciun, Irvin Schultz, Sharon Hook, James Nagler, Tim Cavileer, Joseph Verducci, Yushi Liu, Jonghan Kim, William Hayton

“…Numerical simulation results based on this model are comparedwith experimental data for estrogen receptor mRNA, Vg mRNA, and Vgin the plasma from female rainbow trout over a normal annual reproductivecycle. …”
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Impact of Notch3 Activation on Aortic Aneurysm Development in Marfan Syndrome by Kathryn Jespersen, Chenxin Li, Rishi Batra, Christopher A. Stephenson, Paul Harding, Kylie Sestak, Ryan T. Foley, Harrison Greene, Trevor Meisinger, Jason R. Cook, B. Timothy Baxter, Wanfen Xiong

“…However, the role of Notch signaling in aortic pathology and aortic smooth muscle cell (SMC) differentiation in Marfan syndrome (MFS) is not completely understood. Methods. RNA-sequencing on ascending aortic tissue from a mouse model of MFS, Fbn1mgR/mgR, and wild-type controls was performed. …”
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Inhibition of BRD4 attenuated IFNγ-induced apoptosis in colorectal cancer organoids by Akimi Yonezawa, Kana Shimomura, Koji Okamoto, Haruna Takeda

“…Methods We knocked out Brd4 in tumor organoids carrying mutations in Apc and Kras to generate Brd4KO organoids, and performed RNA-seq. The response of Brd4KO organoids to IFNγ was analyzed via a cell viability assay, an apoptosis assay, and RNAseq. …”
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AURKB affects the proliferation of clear cell renal cell carcinoma by regulating fatty acid metabolism by Yang Yang, Dan Li, Zhigang Liu, Kai Zhou, Wenxing Li, Yanqi Yang, Ruifang Sun, Yulong Li

“…Methods We performed bioinformatics analysis, CCK-8 assay, RNA sequencing, RT-PCR and Western blot to analyze the function and mechanism of AURKB in ccRCC. …”
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The Dissemination of Rift Valley Fever Virus to the Eye and Sensory Neurons of Zebrafish Larvae Is Stat1-Dependent by Sebastiaan ter Horst, Aleksandra Siekierska, Ann-Sofie De Meulemeester, Arno Cuvry, Laura Cools, Johan Neyts, Peter de Witte, Joana Rocha-Pereira

“…Pericardial injection of RVFV resulted in ~4 log₁₀ viral RNA copies/larva, which was inhibited by the antiviral 2′-fluoro-2′-deoxycytidine. …”
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Effects of Aspirin on Odontogenesis of Human Dental Pulp Cells and TGF-β1 Liberation from Dentin In Vitro by V. Khampatee, C. Zhang, L. Chou

“…Expressions of DSP and RUNX2 were determined with the ELISA. DSP and RUNX2 mRNA levels were measured with RT-qPCR. Alizarin red staining was conducted to evaluate the mineralized nodule formation. …”
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A Study Comparing Immunohistochemistry, Fluorescence in situ hybridization, GeneXpert® Breast Cancer STRAT4 Assay for Determining Hormone Receptor, Human Epidermal Growth Factor Re... by Rajaa El Aje, Abdellah Naya, Ayoub Khoaja, Younes Zaid, Mounia Oudghiri, Jodi Weidler, Mehdi Karkouri

“…The simple Kappa coefficient was, for ER, 0.830 (P < 0.0001), 0.565 (P < 0.0001) for PR, 0.838 (P < 0.0001) for HER2-IHC, 0.771 (P < 0.0001) for HER2 IHC+FISH and, for, Ki67, 0.458 (P < 0.0001); Conclusions: We demonstrated globally a high concordance between centrally assessed IHC, IHC+FISH and mRNA measurements of ER/ESR1 and HER2/ERBB2, and a moderate agreement between PR/PGR and Ki67/MKi67. …”
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cAMP analogues downregulate the expression of granulocyte macrophage colony-stimulating factor (GM-CSF) in human bone marrow stromal cells in vitro by G. Bug, J. Aman, C. Huber, C. Peschel, H. G. Derigs

“…Incubation of secondary haematopoietic progenitor cell deprived human stromal cell cultures with IL-1 or TNF- α induced GM-CSF protein expression in culture supernatants and GM-CSFmRNA in adherent stromal cells. The coincubation with 8-bromo-cAMP (8BrcAMP), a water soluble cAMP analogue, inhibited this GM-CSF stimulation at the protein and the m RNA level. …”
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Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines by Raouf Bahri, Sèverine Curt, Dalila Saidane-Mosbahi, Mahmoud Rouabhia

“…Following contact with C. parapsilosis, normal human gingival epithelial cells expressed high levels of Toll-like receptors (TLR)-2, -4, and -6, but not TLR-9 mRNA. The upregulation of TLRs was paralleled by an increase of IL-1β, TNFα, and IFNγ mRNA expression, suggesting the involvement of these cytokines in the defense against infection with C. parapsilosis. …”
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Interference in Macrophage Balance (M1/M2): The Mechanism of Action Responsible for the Anti-Inflammatory Effect of a Fluorophenyl-Substituted Imidazole by Julia Salvan da Rosa, Eduarda Talita Bramorski Mohr, Tainá Larissa Lubschinski, Guilherme Nicácio Vieira, Thais Andreia Rossa, Marcus Mandolesi Sá, Eduardo Monguilhott Dalmarco

“…Moreover, fluorophenyl-imidazole had the ability to increase the M2 markers IL-4, IL-13, CD206, apoptosis and phagocytosis levels, arginase-1, and FIZZ-1 mRNA expression before LPS stimulation. Similarly, it was also able to induce the production of these same M2 markers in macrophages without being induced with LPS. …”
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Modulation of Apoptosis and ER Stress Markers in Hepatocellular Carcinoma Cells by Irinotecan, Hesperidin, and Piperine by Serkan Sen, Kardelen Kocaman Kalkan, Canan Yilmaz, Sefa Celik

“…Researchers performed total RNA extraction and quantitative PCR to assess mRNA levels of Bad, Bax, and p53 (apoptosis-related genes) and ATF4, CHOP, and GRP78 (ER stress markers). …”
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Loss of SNHG4 Attenuated Spinal Nerve Ligation-Triggered Neuropathic Pain through Sponging miR-423-5p by Xia Pan, Cheng Shen, Yayi Huang, Long Wang, Zhongyuan Xia

“…Increasing noncoding RNAs have been implicated in neuropathic pain development. lncRNAs have been recognized as significant regulators of neuropathic pain. lncRNA Small Nucleolar RNA Host Gene 4 (SNHG4) is associated with several tumors. …”
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