Multi-locus CRISPRi targeting with a single truncated guide RNA by Molly M. Moore, Siddarth Wekhande, Robbyn Issner, Alejandro Collins, Anna J. Cruz, Yanjing V. Liu, Nauman Javed, Salvador Casaní-Galdón, Jason D. Buenrostro, Charles B. Epstein, Eugenio Mattei, John G. Doench, Bradley E. Bernstein, Noam Shoresh, Fadi J. Najm

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scRNA‐seq data analysis method to improve analysis performance by Junru Lu, Yuqi Sheng, Weiheng Qian, Min Pan, Xiangwei Zhao, Qinyu Ge

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Exploring microRNA pathways and metformin’s anti‐inflammatory potential in osteoarthritis by Quang D. La, Benjamin Tangkamolsuk, David F. Lo

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Aberrant Regulation of Messenger RNA 3′-Untranslated Region in Human Cancer by Isabel López de Silanes, María Paz Quesada, Manel Esteller

“…The messenger RNA 3′-untranslated region (3′UTR) is emerging as critically important in regulating gene expression at posttranscriptional levels. …”
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Exploiting the Achilles’ Heel of Viral RNA Processing to Develop Novel Antivirals by Ali Zahedi Amiri, Choudhary Ahmed, Subha Dahal, Filomena Grosso, Haomin Leng, Peter Stoilov, Maria Mangos, Johanne Toutant, Lulzim Shkreta, Liliana Attisano, Benoit Chabot, Martha Brown, Mario Huesca, Alan Cochrane

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How to survive the mutational meltdown: lessons from plant RNA viruses by Lafforgue, Guillaume, Lefebvre, Marie, Michon, Thierry, Elena, Santiago F.

“…While the impact of Muller’s ratchet on viruses has been largely studied in bacteriophages and animal RNA viruses, its effects on plant RNA viruses remain poorly documented. …”
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Exosome-Derived microRNA: Potential Target for Diagnosis and Treatment of Sepsis by Yujie Xiao, Yixuan Yuan, Dahai Hu, Hongtao Wang

“…During the progress of sepsis, the miRNA profile of exosomes undergoes notable alterations, is reflecting, and may affect the progression of the disease. …”
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Detection by Single-Cell RNA Sequencing of Virally Mediated Skin Diseases by Linda Zhou, Thomas H. Leung

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Expression and clinical significance of disulfidptosis-related LncRNA in gastric cancer by CHU Yudan, SUN Haidong, CUI Ran, ZHENG Hong

Subjects: “…|gastric cancer|disulfidptosis|disulfidptosis-related long non-coding rna(lncrna)|lncrna-small nucleolar rna host gene (snhg4)|wnt signaling pathway…”
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Mutanome Engineered RNA Immunotherapy: Towards Patient-Centered Tumor Vaccination by Mathias Vormehr, Barbara Schrörs, Sebastian Boegel, Martin Löwer, Özlem Türeci, Ugur Sahin

“…In this review, we describe a process, which integrates next generation sequencing based cancer mutanome mapping, in silico target selection and prioritization approaches, and mRNA vaccine manufacturing and delivery into a process we refer to as MERIT (mutanome engineered RNA immunotherapy).…”
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HIV RNA Suppression and Immune Restoration: Can We Do Better? by Marilia Rita Pinzone, Michelino Di Rosa, Bruno Cacopardo, Giuseppe Nunnari

“…., low levels of plasma HIV RNA) has been related with higher levels of chronic inflammation and all-cause mortality. …”
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Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA by Nico Rublack, Hien Nguyen, Bettina Appel, Danilo Springstubbe, Denise Strohbach, Sabine Müller

“…Nowadays, RNA synthesis has become an essential tool not only in the field of molecular biology and medicine, but also in areas like molecular diagnostics and material sciences. …”
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Genetic Variations and mRNA Expression of NRF2 in Parkinson’s Disease by Caroline Ran, Karin Wirdefeldt, Lovisa Brodin, Mehrafarin Ramezani, Marie Westerlund, Fengqing Xiang, Anna Anvret, Thomas Willows, Olof Sydow, Anders Johansson, Dagmar Galter, Per Svenningsson, Andrea Carmine Belin

“…Further, we quantified NRF2 mRNA expression in EBV transfected human lymphocytes from patients and controls using quantitative real-time reverse transcription PCR. …”
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High-accuracy crRNA array assembly strategy for multiplex CRISPR by Xiangtong Zhao, Lixian Yang, Peng Li, Zijing Cheng, Yongshi Jia, Limin Luo, Aihong Bi, Hanchu Xiong, Haibo Zhang, Hongen Xu, Jinrui Zhang, Yaodong Zhang

Subjects: “…MT: RNA/DNA Editing…”
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Ovarian Cancer Prognostic Prediction Model Using RNA Sequencing Data by Seokho Jeong, Lydia Mok, Se Ik Kim, TaeJin Ahn, Yong-Sang Song, Taesung Park

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Leveraging mRNA technology for antigen based immuno-oncology therapies by Wei Zheng, Michele Ceccarelli, Charalampos S Floudas, Siranush Sarkizova

“…The application of messenger RNA (mRNA) technology in antigen-based immuno-oncology therapies represents a significant advancement in cancer treatment. …”
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Expanding the miRNA Transcriptome of Human Kidney and Renal Cell Carcinoma by Adam P. Sage, Brenda C. Minatel, Erin A. Marshall, Victor D. Martinez, Greg L. Stewart, Katey S. S. Enfield, Wan L. Lam

“…Here, we undertake a large-scale analysis of small RNA-sequencing data from both clear cell renal cell carcinoma (ccRCC) and nonmalignant samples to generate a robust set of miRNAs that remain unannotated in kidney tissues. …”
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Molecular characterization of Pseudomyxoma peritonei with single-cell and bulk RNA sequencing by Ye Jin Ha, Seong-Hwan Park, Seon-Kyu Kim, Ka Hee Tak, Jeong-Hwan Kim, Chan Wook Kim, Yong Sik Yoon, Seon-Young Kim, Jong Lyul Lee

“…Additionally, we provide bulk RNA-seq datasets from two independent cohorts: 19 fresh frozen tissue samples (12 PMPs) and 34 formalin-fixed paraffin-embedded (FFPE) samples (25 PMPs). …”
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The Emerging Functions of Long Noncoding RNA in Immune Cells: Autoimmune Diseases by Keshav Raj Sigdel, Ao Cheng, Yin Wang, Lihua Duan, YanLin Zhang

“…The long noncoding RNAs (lncRNAs) are RNA transcripts more than 200 nucleotides in length, which do not encode proteins. …”
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Identification of prognostic biomarkers of sepsis and construction of ceRNA regulatory networks by Guihong Chen, Wen Zhang, Chenglin Wang, Muhu Chen, Yingchun Hu, Zheng Wang

“…A ceRNA network consisting of 4 circRNA, 26 miRNA, and 2 mRNA was constructed. …”
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