Development and characterization of a double-crested cormorant hepatic cell line, DCH22, for chemical screening by Tasnia Sharin, Doug Crump, Jason M. O’Brien

“…Exposure to 3,3′,4,4′,5-pentachlorobiphenyl (PCB-126), benzo-a-pyrene, ß-napthoflavone and phenacetin induced CYP1A activity and mRNA expression in DCH22 3D spheroids. Induction of CYP3A activity and mRNA expression was observed following exposure to hexabromocyclododecane (HBCD), tris(1,3-dichloroisopropyl)phosphate, carbamazepine, and metyrapone. …”
Get full text

Benchmarking cell type annotation methods for 10x Xenium spatial transcriptomics data by Jinming Cheng, Xinyi Jin, Gordon K. Smyth, Yunshun Chen

“…Many good reference-based cell type annotation tools have been developed for single-cell RNA sequencing and sequencing-based spatial transcriptomics data. …”
Get full text

Potential Biomarkers of the Earliest Clinical Stages of Parkinson’s Disease by Anelya Kh. Alieva, Elena V. Filatova, Aleksey V. Karabanov, Sergey N. Illarioshkin, Petr A. Slominsky, Maria I. Shadrina

“…Based on these results, it can be suggested that the upregulation of the mRNA levels of ATP13A2, PARK7, and ZNF746 in untreated patients in the earliest clinical stages can also be observed in the preclinical stages of PD, and that these genes can be considered as potential biomarkers of the preclinical stage of PD.…”
Get full text

Estimation of Indole-3-Acetic Acid Production from New Molecularly Characterized Rhizobacterial Strains by Mohammed Ayad Harbawi, Najwa Ibrahim Khaleel Al-Barhawee

“… From different fields in Mosul city, we collected sixteen samples of soil surrounding the rhizosphere zone to isolate and characterize rhizobacterial species based on biochemical tests, 16S rRNA gene primer amplification using PCR and nucleotide sequence analysis, and similarity to global isolates in the gene bank. …”
Get full text

The Frequency of Intestinal Parasitic Infections in COVID-19 Patients: A Case-Control Study in Tehran, Capital of Iran by Ali Taghipour, Majid Pirestani, Ramin Hamidi Farahani, Mohammad Barati

“…Nasopharyngeal/oropharyngeal swab samples were collected from all participants for the diagnosis of COVID-19. RNA extraction was performed, and then real time reverse-transcription polymerase chain reaction (rRT-PCR) assay was applied to detect viral RNA. …”
Get full text

Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales by Yusuke Takashima, Kohei Yamamoto, Yousuke Degawa, Yong Guo, Tomoyasu Nishizawa, Hiroyuki Ohta, Kazuhiko Narisawa

“…Phylogenetic analysis of the 16S rRNA gene of BRE revealed that it is distinct from all known BRE. …”
Get full text

Investigating Pb2 CAP-binding domain inhibitors from marine bacteria for targeting the influenza A H5N1. by Taha A Kumosani, Aymn T Abbas, Balogun Basheer, Ahmed M Hassan, Soonham S Yaghmoor, Areej H Alyahiby, Amer H Asseri, Vivek Dhar Dwivedi, Esam I Azhar

“…A promising strategy for antiviral drug development involves targeting the RNA-dependent RNA polymerase, specifically the PB2-cap binding domain of Influenza A H5N1. …”
Get full text

Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene by Ke Li, Lijun Wu, Jianjiang Liu, Wei Lin, Qiang Qi, Tianlan Zhao

“…These data provide a direct evidence that a novel tRNA mutation was associated with T2DM. Thus, our findings provide a new insight into the understanding of pathophysiology of maternally inherited diabetes.…”
Get full text

Activation of TLR4-Mediated NF𝜅B Signaling in Hemorrhagic Brain in Rats by Weiyu Teng, Lishu Wang, Weishuang Xue, Chao Guan

“…Compared with saline control, the TLR4 mRNA and protein significantly increased starting at 6 hours after ICH, peaked on the 3rd day after ICH, and then decreased but still maintained at a higher level on the 7th day after ICH (𝑃<.05). …”
Get full text

Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human Adipocytes by Yuan Tian, Aize Kijlstra, Johan Renes, Martin Wabitsch, Carroll A. B. Webers, Tos T. J. M. Berendschot

“…Lutein significantly inhibited adipocyte FD mRNA expression and FD protein release into adipocyte culture supernatants. …”
Get full text

Reanalysis of sequences of alleged Javan tiger highlights the difficulties in studying big cats and the need for high-throughput sequencing by Anubhab Khan, Yulianto Yulianto, Sabhrina Gita Aninta, Wirdateti Wirdateti

Get full text

Expression and Secretion of CXCL-8 and CXCL-10 From <emph>Mycobacterium Bovis</emph> BCG-Infected Human Epithelial Cells: Role of IL-4

“…We found that, although <emph>M. bovis</emph> BCG induced gene expression of CXCL-8 and CXCL-10 in <emph>M. bovis</emph> BCG-infected human epithelial cells, CXCL-8 mRNA level was significantly reduced by IL-4, whereas no significant effect of IL-4 was observed on CXCL10 mRNA level. …”
Get full text

Effects of Amelogenin on Proliferation, Differentiation, and Mineralization of Rat Bone Marrow Mesenchymal Stem Cells In Vitro by Masanobu Izumikawa, Keijiro Hayashi, Mohammad Ali Akbor Polan, Jia Tang, Takashi Saito

“…The mRNA expression of osteocalcin and DMP-1 was also stimulated for 24 hours and 0.5 hours, respectively, in amelogenin addition group. …”
Get full text

Investigation of the Mechanism Underlying Calcium Dobesilate-Mediated Improvement of Endothelial Dysfunction and Inflammation Caused by High Glucose by Yijun Zhou, Chaojun Qi, Shu Li, Xinghua Shao, Zhaohui Ni

“…A pentraxin 3 (PTX3) small interfering RNA (siRNA) intervention experiment was performed in the cells. …”
Get full text

The potential of supplementing compound organic trace elements at lower levels in Chinese yellow- feathered broiler diets, part II: Impacts on growth performance, gut health, intes... by Xiaoyan Nie, Fei Zhao, Yucheng Yin, Qi Lu, Yang Dai, Rui Wang, Yiwen Ji, Huihua Zhang, Cui Zhu

“…Spearman correlation analysis showed that the mRNA of occludin and jejunal V/C ratio were positively correlated with the abundance of Firmicutes (phylum), but negatively correlated with the abundance of Bacteroidota (phylum). …”
Get full text

The assessment of marrow adiposity in type 1 diabetic rabbits through magnetic resonance spectroscopy is linked to bone resorption by Wei Li, Wei Wang, Minlan Zhang, Qi Chen, Fengyi Li, Shaojun Li

“…Marrow adipogenesis was evident through a 31.0% increase in adipocyte size, a 60.0% rise in adipocyte number, a 103.3% increase in the percentage of adipocyte area, and elevated mRNA expressions of marrow adipocyte markers. Osteoclast markers (TRACP and cathepsin K RNA and serum TRACP5b levels) were elevated in diabetic rabbits. …”
Get full text

To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway by Jia-Rong Gao, Xiu-Juan Qin, Zhao-Hui Fang, Li-Shan, Li-Ping Han, Hui-Jiang, Ming-Fei Guo, Nan-Nan Jiang

“…RT-QPCR was used to detect the mRNA expression of endothelial markers PTEN, PI3K, Akt, and VEGF. …”
Get full text

Identification of Anoikis-Related Genes in Chronic Kidney Disease Based on Bioinformatics Analysis Combined with Experimental Validation by Liu H, Mei M, Zhong H, Lin S, Luo J, Huang S, Zhou J

“…Furthermore, we constructed a competitive endogenous RNA (ceRNA) network for the hub genes utilizing the ENCORI and miRDB databases, while also calculating Spearman correlation coefficients. …”
Get full text

Exosomes secreted by ATF3/Nrf2-mediated ferroptotic renal tubular epithelial cells promote M1/M2 ratio imbalance inducing renal interstitial fibrosis following ischemia and reperfu... by Qiao Tang, Jiatao Xie, Yifei Wang, Chong Dong, Chong Dong, Qian Sun

“…The IR group had more significant mitochondrial damage and higher PTGS2 and TFRC mRNA levels than those in the sham group. Compared with the IR group, the above indexes were all alleviated in the IR+Fer-1 or IR+ATF3KD groups. …”
Get full text

Repressor Element 1 Silencing Transcription Factor (REST) Governs Microglia-Like BV2 Cell Migration via Progranulin (PGRN) by Tongya Yu, Yingying Lin, Yuzhen Xu, Yunxiao Dou, Feihong Wang, Hui Quan, Yanxin Zhao, Xueyuan Liu

“…REST expression was inhibited by sh-RNA, which induced BV2 cell migration obviously. …”
Get full text