Comprehensive review of preclinical evaluation strategies for COVID-19 vaccine candidates: assessing immunogenicity, toxicology, and safety profiles by Delaram Doroud, Mojtaba Daneshi, Fatemeh Kazemi-Lomedash, Zohre Eftekhari

“…Evaluating their biodistribution is crucial for newly developed vaccines, such as lipid nanoparticles –messenger RNA (LNP-mRNA), DNA, and Viral vectors in non-replicated (VVnr), or recombinant virus vaccines. …”
Get full text

Zerumbone Attenuates the Severity of Acute Necrotizing Pancreatitis and Pancreatitis-Induced Hepatic Injury by Deng Wenhong, Yu Jia, Wang Weixing, Chen Xiaoyan, Chen Chen, Xu Sheng, Jin Hao

“…The expression of I-κBα and NF-κB protein was evaluated by western blot and immunohistochemistry assay while ICAM-1 and IL-1β mRNA were examined by RT-PCR. The results showed that AMY, sPLA2, ALT, and AST levels and histopathological assay of pancreatic and hepatic tissues were significantly reduced following administration of zerumbone. …”
Get full text

Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages by Ulla Jalonen, Riina Nieminen, Katriina Vuolteenaho, Hannu Kankaanranta, Eeva Moilanen

“…We established a TTP knock-down cell line by expressing shRNA against TTP (shTTP cell line). A cytokine antibody array was used to measure cytokine production in macrophages exposed to lipopolysaccharide (LPS). …”
Get full text

Peptidoglycan recognition protein PGRP-5 is involved in immune defence and neuro-behavioral disorders in zebrafish embryos. by Xi Li, Guanghua Xiong, Manni Luo, Siwan Mao, Ruiying Zhang, Ziwei Meng, Juan Li, Xinjun Liao

“…Thirdly, the mRNA levels of DrPGRP-5 were greatly elevated after 6 h of E. coli infection but reached its highest value at 24 h after M. luteus stimulation. …”
Get full text

Sequential Treatment with Activin and Hepatocyte Growth Factor Induces FOXM1 to Promote Colorectal Cancer Liver Metastasis by Hong Peng, Ting Ye, Lei Deng, Xiaofang Yang, Zheng Jiang, Jinjun Guo

“…Activin alone increased the mRNA and protein expression of FOXM1. In contrast, HGF alone enhanced the phosphorylation of FOXM1, without altering the total protein level of FOXM1. …”
Get full text

Distribution of Hepatitis C Virus Genotypes in Canada: Results from the LCDC Sentinel Health Unit Surveillance System by RK Chaudhary, M Tepper, S Eisaadany, Paul R Gully

“…Samples from 387 patients (40.4%) were tested for HCV RNA, and 289 (74.7%) were positive for RNA. The major risk factor for HCV infection was injection drug use, reported in 71% of cases. …”
Get full text

Complete chloroplast genome of Sagina maxima A. Gray (Caryophyllaceae) from Ulleung-do Island, Korea by Asif S. Tamboli, Suhas K. Kadam, Jin-Suk Youn, JiYoung Yang, Young Seok Sim, Jae Hong Pak, Yeon-Sik Choo

“…A unique set of 112 genes were identified which includes 78 protein-coding genes, 30 tRNA and 4 rRNA. Total 18 genes were duplicated within the IR regions and 16 genes have one intron and only clpP gene with two introns. …”
Get full text

Postharvest Analysis of Lowland Transgenic Tomato Fruits Harboring hpRNAi-ACO1 Construct by Bita Behboodian, Zainon Mohd Ali, Ismanizan Ismail, Zamri Zainal

“…The plant hormone, ethylene, is an important regulator which involved in regulating fruit ripening and flower senescence. In this study, RNA interference (RNAi) technology was employed to silence the genes involved in ethylene biosynthetic pathway. …”
Get full text

Modulatory Impacts of Probiotic on the Synthesis of Claudins and Mucins in the Gastrointestinal Tract of Broiler Chicks by ESI Mohammed, M Al-Rasheed, MS Ahmed

“…ABSTRACT This study investigated the effect of 3 strains of probiotic bacteria on mRNA synthesis of some claudins (claudins1(CLD1), CLD3, and CLD5), and mucins (MUC5AC and MUC2) in broilers’ digestive systems. …”
Get full text

Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR by Wan Fen Joveen-Neoh, Ka Lung Chong, Clemente Michael Vui Ling Wong, Tiek Ying Lau

“…The objectives of this paper were to accurately identify the incidence of human malaria parasites in the interior division of Sabah, Malaysian Borneo, based on small subunit ribosomal RNA (ssrRNA) and to determine the misidentification rate in human malaria parasites. …”
Get full text

Isolation and Characterization of Diesel-Degrading Bacteria from Hydrocarbon-Contaminated Sites, Flower Farms, and Soda Lakes by Gessesse Kebede Bekele, Solomon Abera Gebrie, Eshetu Mekonen, Tekle Tafese Fida, Adugna Abdi Woldesemayat, Ebrahim M. Abda, Mesfin Tafesse, Fasil Assefa

“…Consequently, the partial 16S rRNA gene sequences revealed that the two most potent bacterial strains (AAUW23 and AAUG11) were Pseudomonas aeruginosa, while AAUG36 was Bacillus subtilis. …”
Get full text

Blue light promotes conjunctival epithelial-mesenchymal transition and collagen deposition through ITGB4 by Qianjie Yang, Kuangqi Chen, Siyi Chen, Yinhao Wang, Yutong Xia, Jinbo Chen, Ye Shen

“…BL disrupted cell conjunction and regulated EMT-related proteins. RNA sequencing analysis revealed upregulation of ITGB4 and enrichment of cell migration and adhesion pathways. …”
Get full text

Exosomal circRNAs: Deciphering the novel drug resistance roles in cancer therapy by Xi Li, Hanzhe Liu, Peiyu Xing, Tian Li, Yi Fang, Shuang Chen, Siyuan Dong

“…Exosomal circular RNA (circRNAs) are pivotal in cancer biology, and tumor pathophysiology. …”
Get full text

Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma by Chao Lu, Shenglin Gao, Li Zhang, Xiaokai Shi, Yin Chen, Shuzhang Wei, Li Zuo, Lifeng Zhang

“…We applied lentivirus-mediated small-interfering RNA to down-regulate the expression of Nuclear protein 1 in BTCC cell lines. …”
Get full text

TOM1L Is Involved in a Novel Signaling Pathway Important for the IL-2 Production in Jurkat T Cells Stimulated by CD3/CD28 CoLigation by Ahmed Elmarghani, Hanan Abuabaid, Peter Kjellen

“…Here we show that in Jurkat T cells stimulated by CD3/CD28 coligation where the expression of TOM1L is reduced by lenti virus mediated-siRNA results in a dramatically lower IL-2 production. …”
Get full text

Spatiotemporal Pattern of Neuroinflammation After Impact-Acceleration Closed Head Injury in the Rat by Servan Rooker, Sebastian Jander, Jos Van Reempts, Guido Stoll, Philippe G. Jorens, Marcel Borgers, Jan Verlooy

“…We found an early upregulation of IL-1β, IL-6, and TNF-α mRNA between 1 h and 7 h after injury; the expression of iNOS mRNA only revealed a significant increase at 4 h. …”
Get full text

Carvedilol Confers Ferroptosis Resistance in HL-1 Cells by Upregulating GPX4, FTH1, and FTL1 and Inducing Metabolic Remodeling Under Hypoxia/Reoxygenation by Yi-Chin Li, Mei-Ling Cheng

“…Also, Carvedilol promoted ferroptosis-related genes, ferritin heavy chain 1 (<i>FTH1</i>) and ferritin light chain 1 (<i>FTL1</i>) mRNA levels, to reduce lipid peroxidation under both hypoxia and HR. …”
Get full text

Modulatory effects of rutin on the expression of cytochrome P450s and antioxidant enzymes in human hepatoma cells by Karakurt Sedar

“…Besides, rutin treatment significantly inhibited mRNA expression of CYP3A4 (73.2 %, p=0.0014). Also, CYP1A1, NQO1 and GSTP1 mRNA expressions were significantly increased 2.77-fold (p = 0.029), 4.85- fold (p = 0.0051) and 9.84-fold (p < 0.0001), respectively.…”
Get full text

The R203M and D377Y mutations of the nucleocapsid protein promote SARS-CoV-2 infectivity by impairing RIG-I-mediated antiviral signaling. by Yongkui Li, Moran Li, Heng Xiao, Feng Liao, Miaomiao Shen, Weiwei Ge, Junxian Ou, Yuqing Liu, Lumiao Chen, Yue Zhao, Pin Wan, Jinbiao Liu, Jun Chen, Xianwu Lan, Shaorong Wu, Qiang Ding, Geng Li, Qiwei Zhang, Pan Pan

“…Those mutations, R203M and D377Y, enhanced the inhibitory impact of N protein on the impairment of RIG-I-mediated antiviral signaling, such as IRF3 phosphorylation and IFN-β activation. The viral RNA-binding activity of N protein was promoted by these mutations, effectively attenuating the recognition and interaction of RIG-I with viral RNA compared to the original or other variants. …”
Get full text

Markers of chronic hepatitis B at patients with HIV-infection and treatment approaches by S. I. Maksimov, S. P. Tsarenko, A. V. Kravchenko, N. Yu. Gankina, N. D. Yushchuk

“…All blood samples were investigated for the presence of НBV, HDV, HCV, HAV-virus markers in serum by enzyme-linked immunoassay and for the presence of RNA HIV, RNA HCV and DNA HBV by polymerase chain reaction.Results. …”
Get full text