Characterization and Study of Gene Expression Profiles of Human Periodontal Mesenchymal Stem Cells in Spheroid Cultures by Transcriptome Analysis by Takenori Suga, Michihiko Usui, Satoru Onizuka, Kotaro Sano, Tsuyoshi Sato, Kohji Nakazawa, Wataru Ariyoshi, Tatsuji Nishihara, Keisuke Nakashima

“…Interestingly, we found that the mRNA and protein expressions of NR4A2 were strongly expressed in the spheroid-cultured hPDLMSCs. …”
Get full text

An atlas of neuropathic pain-associated molecular pathological characteristics in the mouse spinal cord by Fu-Lu Dong, Lina Yu, Pei-Da Feng, Jin-Xuan Ren, Xue-Hui Bai, Jia-Qi Lin, De-Li Cao, Yu-Tao Deng, Yan Zhang, Hui-Hui Shen, Hao Gong, Wen-Xing Sun, Dong-Qiu Chi, Yixiao Mei, Longfei Ma, Ming-Zhe Yin, Meng-Na Li, Peng-Fei Zhang, Nan Hu, Bing-Lin Zhou, Ying Liu, Xuan-Jie Zheng, Yi-Fan Chen, Da Zhong, Yuan-Xiang Tao, Min Yan, Bao-Chun Jiang

“…We here integrate spatial transcriptomics (ST) with single-nucleus RNA-sequencing (snRNA-seq) and bulk RNA-sequencing (bulk RNA-seq) to characterize regional pathological heterogeneity of the SC under NP conditions. …”
Get full text

Hypoglycemic effects of black brick tea with fungal growth in hyperglycemic mice model by Wei Xu, Yang Zhou, Ling Lin, Dongyin Yuan, Yingqi Peng, Li Li, Wenjun Xiao, Zhihua Gong

“…These teas can promote blood glucose transport and absorption by upregulating the protein and mRNA expression of insulin receptor substrate-1 and glucose transporter-2 and stimulate glycolysis by upregulating the protein and mRNA expression of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2. …”
Get full text

Two Novel Compounds Isolated from the Marine Fungal Symbiont of Aspergillus unguis Induce Apoptosis and Cell Cycle Arrest in Breast Cancer Cells: In vitro Study by Bashari MH, Agung MUK, Ariyanto EF, Al Muqarrabun LMR, Salsabila S, Chahyadi A, Rosandy AR, Afifah E, Afni M, Atmaja HE, Putri T, Utami F, Subhan B, Syafrizayanti, Andriani Y, Elfahmi

“…Unguisol A and Unguisol B induced apoptosis in MDA-MB-231 breast cancer cells via BCL2L1 mRNA downregulation, while both compounds induced cell cycle arrest at the S phase through AKT1 mRNA downregulation. …”
Get full text

Phenome-wide association studies between SERINC2 and neuropsychiatric disorders by Ping Liu, Xinqun Luo, Liping Cao, Yong Zhang, Jiawu Ji, Xiaoping Wang, Kesheng Wang, Xinghua Pan, Ruilan Yang, Zewen Tan, Yunlong Tan, Chiang-shan Li, Xiaoyun Guo, Zhiren Wang, Xingguang Luo, Xingguang Luo

“…Furthermore, the disease-risk alleles had highly similar regulatory effects on mRNA expression (8.1×10-7≤p ≤ 0.046), subcortical GMVs (7.0×10-4≤p ≤ 0.048) and cortical TH and SA (1.3×10-3≤p ≤ 0.050) in brains across alcoholism, schizophrenia, OCD and autism. …”
Get full text

Kloning gen virulen Streptococcus agalactiae sebagai bahan dasar vaksin rekombinan by , Sutanti, Retno Damayanti Soejoedono, Irvan Faizal

“…Bacterial identification was accomplished by PCR, using 16S rRNA primers, which revealed the 1,500 bp PCR product. …”
Get full text

NEAT1 regulates BMSCs aging through disruption of FGF2 nuclear transport by Zifei Wang, Wenyu Zhen, Qing Wang, Yuqiang Sun, Siyu Jin, Sensen Yu, Xing Wu, Wenhao Zhang, Yulong Zhang, Fei Xu, Rui Wang, Yuxuan Xie, Wansu Sun, Jianguang Xu, Hengguo Zhang

“…Abstract Background The aging of bone marrow mesenchymal stem cells (BMSCs) impairs bone tissue regeneration, contributing to skeletal disorders. LncRNA NEAT1 is considered as a proliferative inhibitory role during cellular senescence, but the relevant mechanisms remain insufficient. …”
Get full text

The Response of Corneal Endothelial Cells to Shear Stress in an In Vitro Flow Model by Sujuan Duan, Yingjie Li, Yanyan Zhang, Xuan Zhu, Yan Mei, Dongmei Xu, Guofu Huang

“…The expression of the corneal endothelium-related markers ZO-1, N-cadherin, and Na+-K+-ATPase in rabbit corneal endothelial cells (RCECs) was upregulated at both the mRNA and protein levels after exposure to shear stress. …”
Get full text

Characterization of the Emerging Enteropathogen Escherichia Albertii Isolated from Urine Samples of Patients Attending Sapporo Area Hospitals, Japan by Miyuki Fujioka, Sho Yoshioka, Masahiko Ito, Chowdhury Rafiqul Ahsan

“…In 16S rRNA sequencing analysis, the E. albertii strains were ranked at the top of homology, but not in the top one, making it difficult to differentiate them from other strains. …”
Get full text

Tissue Factor Expression in Obese Type 2 Diabetic Subjects and Its Regulation by Antidiabetic Agents by Jing Wang, Theodore P. Ciaraldi, Fahumiya Samad

“…Plasma TF antigen, activity, and adipose TF mRNA were greater in obese T2D subjects compared with obese nondiabetics. …”
Get full text

COVID-19: Gastrointestinal Manifestations and Complications by Angel Yun-Kuan Thye, Priyia Pusparajah, Loh Teng-Hern Tan, Jodi Woan-Fei Law, Vengadesh Letchumanan, Learn-Han Lee

“…This review aims to study the prevalence of some of the gastrointestinal manifestations following COVID-19 infection and findings of positive SARS-CoV-2 RNA in stool specimens while making parallels to the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) infection. …”
Get full text

Stochastic modelling of PTEN regulation in brain tumors: A model for glioblastoma multiforme by Margherita Carletti, Matteo Montani, Valentina Meschini, Marzia Bianchi, Lucia Radici

“…Starting from [23,24,25], we introduce a competitive stochastic model for post-transcriptional regulation of PTEN, including interactions with the miRNA and concurrent genes. Our model also covers protein formation and the backward mechanism going from the protein back to the miRNA.The numerical simulations show that the model reproduces the expected dynamics of normal glial cells. …”
Get full text

An Alternative to the Human Hemoglobin Test in the Investigation of Bloodstains Treated with Active Oxygen: The Human Glycophorin A Test by Ana Castelló, Francesc Francés, Fernando Verdú

“…The need to determine the nature of the evidence is reflected in the latest bibliography, where the possibility of employing mRNA and miRNA markers for this purpose is proposed. …”
Get full text

HBV cccDNA: The Molecular Reservoir of Hepatitis B Persistence and Challenges to Achieve Viral Eradication by André Boonstra, Gulce Sari

“…The cccDNA serves as the sole template for viral replication, as it generates the pregenomic RNA (pgRNA) necessary for producing new viral genomes. …”
Get full text

Pituitary adenoma: important genetic factors and clinical diagnostic issues by G. Juknytė, E. Karinauskė, R. Liutkevičienė

“…Since the development of pituitary adenomas can be significantly influenced by epigenetic changes in pituitary cells, there is currently an increasing focus on the study of epigenetic mechanisms such as DNA methylation, histone modifications, and micro-RNA studies. Analysis of mRNA expression is also performed to assess the decrease or increase in gene expression in the case of pathology. …”
Get full text

Comparative analysis of the Streptococcus pneumoniae competence development in vitro versus in vivo during pneumonia-derived sepsis by Sook Yin Chong, Shi Qian Lew, Tauqeer Alam, Christopher A. Gaulke, Gee W. Lau

“…However, mechanisms driving competence development during host infection are rarely examined, and a direct comparison between in vitro and in vivo competence induction has not been performed.MethodsWe conducted a comparative gene expression analysis of pneumococcal competence development in vitro versus in vivo during pneumonia-derived sepsis in mice. We examined existing RNA-Seq data and performed validation using RNA obtained from an independent replicate experiment.Results and discussionOur analysis revealed both similarities and differences in the expression of “early”, “late”, and “delayed” competence between in vitro versus during pneumonia-derived sepsis. …”
Get full text

Chronic asymptomatic orchitis in dogs alters Sertoli cell number and maturation status by Pauline Rehder, Eva-Maria Packeiser, Hanna Körber, Sandra Goericke-Pesch

“…Sertoli cells also partly returned to an immature status, as indicated by the expression of AMH and CK18 at mRNA and protein levels. The degree of spermatogenesis disruption matched with the degree of Sertoli cell alterations. …”
Get full text

Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog by Korakot Nganvongpanit, Waranee Pradit, Siriwadee Chomdej

“…Ten mRNA, including nonapoptotic genes (AGG, COL2A1, MMP-3, HAS-1, HAS-2, and TIMP-1) and apoptotic genes (BAX, BCL-2, CAS-3, and CAS-9), were studied for their expression using real-time PCR. …”
Get full text

Altered Peroxisome-Proliferator Activated Receptors Expression in Human Endometrial Cancer by Paweł Knapp, Adrian Chabowski, Agnieszka Błachnio-Zabielska, Katarzyna Jarząbek, Sławomir Wołczyński

“…We sought to analyze PPARs mRNA content as well as protein immunohistochemical expression that was further quantified by Western Blot technique. …”
Get full text

Integrin-Linked Kinase: A Multi-functional Regulator Modulating Extracellular Pressure-Stimulated Cancer Cell Adhesion through Focal Adhesion Kinase and AKT by Shouye Wang, Marc D. Basson

“…We therefore hypothesized that ILK might also regulate pressure-stimulated cancer cell adhesion through AKT and FAK phosphorylation. Silencing ILK by siRNA reduced basal cancer cell adhesion and prevented the stimulation of adhesion by pressure. …”
Get full text