Leptospirosis Risk Assessment in Rodent Populations and Environmental Reservoirs in Humanitarian Aid Settings in Thailand by Panadda Krairojananan, Kasima Wasuworawong, Surachai Leepitakrat, Taweesak Monkanna, Elizabeth W. Wanja, Silas A. Davidson, Betty K. Poole-Smith, Patrick W. McCardle, Alyssa Mann, Erica J. Lindroth

“…A surveillance program was conducted at Engineering Civil Assistance Program (ENCAP) training sites using real-time PCR, dark-field microscopy, and 16S rRNA gene sequencing to detect <i>Leptospira</i> in rodents and environmental samples. …”
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Genetic characterization of clinical <I>Klebsiella</I> isolates circulating in Novosibirsk by A. V. Bardasheva, N. V. Fomenko, T. V. Kalymbetova, I. V. Babkin, S. O. Chretien, E. V. Zhirakovskaya, N. V. Tikunova, V. V. Morozova

“…Species identification of strains was performed using 16S rRNA and rpoB gene sequences. It was revealed that Klebsiella pneumoniae strains were dominant in the population (57 strains), while the remaining 15 strains were K. grimontii, K. aerogenes, K. oxytoca and K. quasipneumoniae. …”
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Exploring the impact of genetic factors and fatty acid quality on visceral and overall Fat levels in overweight and obese women: a genetic risk score study by Niloufar Rasaei, Atefeh Tavakoli, Saba Mohammadpour, Mehdi Karimi, Alireza Khadem, Azam Mohamadi, Seyedeh Fatemeh Fatemi, Fatemeh Gholami, Khadijeh Mirzaei

“…Finally, the GRS was created by combining three SNPs [CAV-1 (rs3807992), Cry-1 (rs2287161), and MC4R (rs17782313)]. Results The study found that there was no significant association between the quality of fat intake (as measured by CSI score and N6/N3 score) and VAI or BAI in both crude (B = 70.70, SE = 35.14, CI:1.81-139.55, P = 0.04) and adjusted models (B = 93.67, SE = 39.28, CI:16.68–17.68, P = 0.01). …”
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Multidisciplinary nursing care in chronic Chagas disease: a scoping review by Ângelo Antônio Oliveira Silva, Leonardo Maia Leony, Ramona Tavares Daltro, Emily Ferreira Santos, Natália Erdens Maron Freitas, Larissa de Carvalho Medrado Vasconcelos, Daniel Dias Sampaio, Faber Neves Santos, Lívia Dórea Dantas Fernandes, Roque Aras, Alejandro Marcel Hasslocher-Moreno, Fred Luciano Neves Santos

“…Methods This scoping review employed the PRISMA-ScR method as a framework for article selection. …”
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Collection of microorganisms of ICG SB RAS as a genetic resource for biotechnology by A. V. Bryanskaya, Y. E. Uvarova, A. S. Rozanov, N. M. Slynko, V. N. Shlyakhtun, K. V. Starostin, E. A. Demidov, E. V. Lazareva, O. P. Taran, S. E. Peltek

“…DNA isolation and the sequencing of 16S rRNA gene sequences were performed. The strains of the collection were characterized by morphological, physiological, moleculargenetic and mass-spectrometric characteristics. …”
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Carbon Stock and Soil Characteristics under Expansive Shrubs in the Dry Afromontane Forest in Northern Ethiopia by Mebrahtu Haile, Emiru Birhane, Meley Mekonen Rannestad, Muyiwa S. Adaramola

“…The statistical difference in carbon stock and soil characteristics P<0.05 of both invaded and uninvaded vegetation conditions were tested using an independent t-test using an R-software. The mean above- and below-ground biomass carbon stocks of the uninvaded vegetation condition (17.62 Mg·C/ha and 4.14 Mg·C/ha, respectively) were found to be significantly higher than those of the invaded vegetation condition (4.73 Mg·C/ha and 1.11 Mg·C/ha, respectively). …”
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Development of High-performance Proton Transfer Reaction Time-of-Flight Mass Spectrometer by Chun-guang XIE, Ji-yang ZHANG, Tong YANG, Yu-liang HUANG, Shu-guang XIE, Hui ZHU, Jing-xuan SUN, Ping CHENG

“…Experimental tests showed that the system’s resolution (full width half maximum, FWHM) is better than 2 069 (for toluene), with the detection limit of 0.36 μg/m3, a linear range of 4 orders of magnitude, and a linear correlation coefficient (R2) of 0.991. The development of this system provides a new method for online rapid analysis of VOCs in environmental monitoring, industrial processes, and biomedicine, accelerating the technical development of PTR-TOFMS domestically and narrowing the gap with the international advanced level.…”
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Increased CCR7loPD-1hiCXCR5+CD4+ T Cells in Peripheral Blood Mononuclear Cells Are Correlated with Immune Activation in Patients with Chronic HBV Infection by Ya-Xin Huang, Qi-Yi Zhao, Li-Li Wu, Dong-Ying Xie, Zhi-Liang Gao, Hong Deng

“…We observed a weak positive correlation between the CCR7loPD-1hi Tfh subset population and the alanine transaminase (ALT) level (r=0.370, p=0.001). The CCR7loPD-1h Tfh subset in the chronic HBV-infected patients was elevated to various degrees among the different immune phases. …”
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Detection of Bartonella spp. in foxes' populations in Piedmont and Aosta Valley (NW Italy) coupling geospatially-based techniques by Annalisa Viani, Tommaso Orusa, Sara Divari, Stella Lovisolo, Stefania Zanet, Riccardo Orusa, Enrico Borgogno-Mondino, Enrico Bollo

“…Sequencing results identified Bartonella schoenbuchensis R1 as the most commonly isolated species (5/9, 62.5%), followed by Candidatus “Bartonella gerbillinarum” in two samples (2/9, 28.6%). …”
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Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal Ecosystem by Michelle Scriver, Ulla vonAmmon, Xavier Pochon, Martin Zirngibl, Francois Audrezet, Kaeden Leonard, Kylie Pedersen, Nick Bamford, Ngairo Tahere, Jo‐Ann L. Stanton, Neil J. Gemmell, Anastasija Zaiko

“…Detected eukaryotic communities were assessed using eDNA metabarcoding analysis of the small‐subunit ribosomal RNA (18S rRNA) and mitochondrial Cytochrome C Oxidase subunit I (COI) genes. …”
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Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Eff... by Anastasia Latanova, Stefan Petkov, Yulia Kuzmenko, Athina Kilpeläinen, Alexander Ivanov, Olga Smirnova, Olga Krotova, Sergey Korolev, Jorma Hinkula, Vadim Karpov, Maria Isaguliants, Elizaveta Starodubova

“…Perforin secretion correlated with coproduction of IFN-γ and IL-2 (R=0,97). Both DNA immunogens induced strong anti-RT antibody response. …”
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Undernutrition disrupts jejunal and ileal microbiota and epithelial tissue homeostasis in a pregnant sheep model by Weibin Wu, Muhammad Faheem Akhtar, Jiahong Geng, Huizhen Lu, Muhammad Ajwad Rahim, Jianbo Cheng, Xiaoling Ding, Shengyong Mao, Yanfeng Xue

“…After 15-d treatment, all ewes were slaughtered, and jejunal and ileal digesta and epithelium samples were collected for 16S rRNA gene sequencing and transcriptome sequencing, respectively. …”
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Identification of the Transcriptional Networks and the Involvement in Angiotensin II-Induced Injury after CRISPR/Cas9-Mediated Knockdown of Cyr61 in HEK293T Cells by Junjie Wang, Dongdong Fu, Soulixay Senouthai, Yan Jiang, Rentong Hu, Yanwu You

“…The differentially expressed genes were affected for small cell lung cancer, axon guidance, Fc gamma R-mediated phagocytosis, MAPK signaling pathway, focal adhesion, insulin resistance, and metabolic pathways. …”
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Comprehensive investigation of matrix metalloproteinases in skin cutaneous melanoma: diagnostic, prognostic, and therapeutic insights by Lingxia Wu, Chenxiaoxiao Liu, Weicai Hu

“…Furthermore, we elucidated potential regulatory pathways involving miR-22-3p, which targets MMP7, MMP11, and MMP14 genes in SKCM. …”
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Impact of the severity and extension of dental caries lesions on Turkish preschool children’s oral health-related quality of life: a cross-sectional study by Ayça Kurt, Damla Bolat, Ömer Hatipoğlu

“…Spearman correlation analysis revealed a significant positive correlation between pufa and ECOHIS scores (r = 0.33, p < 0.05). Conclusions The study demonstrates that extension of dental caries lesions, particularly when measured by the pufa Index, significantly impacts specific aspects of OHRQoL, including pain, eating difficulties, and sleep disturbances in children, as well as emotional distress in parents. …”
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Efficacy and safety of dexamethasone or triamcinolone in combination with anti-vascular endothelial growth factor therapy for diabetic macular edema: A systematic review and meta-a... by Bo Zhou, Hua Liu, Feng Xiong

“…All analyses were performed by R 4.3.1, Stata 12.0, and TSA v0.9.5.10 Beta software.…”
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Assessment of the variability and correlation of biometric measurements in eyes with cataracts by Jian-Feng Zhao, Chen Yang, Jie Zhou, Hai-Yan Zhang, Yu Geng

“…Pearson's correlation coefficients (r) were used to evaluate the relationships between age, gender, and various ocular parameters, including axial length (AL), anterior chamber depth (ACD), lens thickness (LT), central corneal thickness (CCT), corneal refractive power (Km), and white-to-white (WTW) measurement. …”
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Continuous high-soy protein soymilk intake affects ordinary walking speed in the Japanese pre-frail and frail elderly: a randomized controlled trial by Nene Sato, Yuji Terashima, Makoto Sugawara, Ryoichi Unno, Hiroaki Asao, Mitsuhiro Iwasaki, Tomoyuki Watanabe, Tomoko Uno, Mitsuo Maruyama

“…Registered July 29, 2021; https://center6.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000051409 .…”
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Mechanistic insights into chloride‐related heart failure progression according to the plasma volume status by Hajime Kataoka

“…For the study subjects as a whole (n = 47), changes in the ePVS correlated positively with changes in the serum chloride concentration from stable to worsening HF (r = 0.398, P = 0.0056). When divided into two groups of worsening HF with an increased (n = 31) vs. non‐increased serum chloride concentration (n = 16), no significant baseline differences in body weight, serum logBNP, or ePVS were detected between groups. …”
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Enabling regulatory policy globally will promote realization of the potential of animal biotechnology by Eric Hallerman, Justin Bredlau, Luiz Sergio A. Camargo, Maria Lucia Zaidan Dagli, Margaret Karembu, Daniel Kovich, Anne Ndanu Muia, Mariana L. Murrone, Pedro Jesus Rocha-Salavarrieta, Rhodora Romero-Aldemita, Mark Tizard, Mark Walton, Diane Wray-Cahen

“…Only one biotech animal in the world that was developed for food production has reached the market under a GMO or rDNA approval process. The advent of genome editing techniques has revolutionized the scientific approach to introducing changes into DNA sequences and how biotechnology can be used to enhance agricultural breeding. …”
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