Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa by Noel-David Nogbou, Granny M. Nkawane, Khanyisa Ntshane, Charles K. Wairuri, Dikwata T. Phofa, Kagiso K. Mokgokong, Mbudzeni Ramashia, Maphoshane Nchabeleng, Lawrence C. Obi, Andrew M. Musyoki

“…Conventional PCR amplification of the AdeABC efflux pump (adeB, adeS, and adeR) and quinolone (parC and gyrA) resistance genes were performed, followed by quantitative real-time PCR of AdeABC efflux pump genes. …”
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Unraveling nitrogen metabolism, cold and stress adaptation in polar Bosea sp. PAMC26642 through comparative genome analysis by Anamika Khanal, Anamika Khanal, So-Ra Han, So-Ra Han, So-Ra Han, Jun Hyuck Lee, Tae-Jin Oh, Tae-Jin Oh, Tae-Jin Oh, Tae-Jin Oh

“…Enzymes associated with various stress adaptation pathways, including oxidative stress (superoxide dismutase, catalase, and thiol peroxidase), osmotic stress (OmpR), temperature stress (Csp and Hsp), and heavy metal resistance, were also identified. …”
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A Realistic Approach to Calculating the Nitrogen Use Efficiency Index in Cereals with Winter Wheat (<i>Triticum aestivum</i> L.) as an Example by Witold Grzebisz, Jarosław Potarzycki

“…These core datasets were used to calculate total N input (<i>N_in</i>) to the soil/crop system during the two main periods of WW growth: (1) before (vegetative mega-phase, V) and (2) after wheat flowering (reproductive mega-phase, R, or grain filling period, GFP). The number of grains per ear (GE) and the number of grains per unit area (grain density: GD) depended significantly on <i>N_in</i> at the onset of flowering. …”
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Insights gained through real-time monitoring of porcine reproductive and respiratory syndrome virus and description of temporal trends based on laboratory data in Ontario, Canada by Tatiana Petukhova, Maria Spinato, Tanya Rossi, Michele T. Guerin, Cathy A. Bauman, Pauline Nelson-Smikle, Davor Ojkic, Zvonimir Poljak

“…The test results from Ontario swine herd samples submitted from January 2014 to July 2023 were processed in R v.4.1.1. The final optimized, aggregated, and anonymized datasets were exported to the Tableau server and were used to design dynamic real-time visualizations with Tableau Desktop v.2021.4. …”
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A novel multi-source data-driven energy consumption prediction model for Venlo-type greenhouses in China by Yangda Chen, Aiqun Bao, Yapeng Li, Yingfeng Xiang, Wanlong Cai, Zhaoqiang Xia, Jialei Li, Mingyang Ning, Jing Sun, Haixi Zhang, Xianpeng Sun, Xiaoming Wei

“…The final experimental results indicated that the proposed energy consumption prediction method demonstrates excellent performance with high prediction accuracy (R² = 0.9388) and low computational resource consumption (runtime = 926.91s), outperforming other models. …”
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Comparison of different severe obesity definitions in predicting future cardiometabolic risk in a longitudinal cohort of children by Gilles Paradis, Melanie Henderson, Lisa Kakinami, Angelo Tremblay, Anna Smyrnova

“…Unfavourable blood pressure was defined as ≥90th percentile for age-adjusted, sex-adjusted and height-adjusted systolic or diastolic blood pressure.Analysis Area under the receiver operating characteristic curve (AUC) and McFadden psuedo R2 for predicting F1 or F2 unfavourable cardiometabolic levels from baseline SO definitions were calculated. …”
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CRISPR/Cas9-editing of PRNP in Alpine goats by Aurélie Allais-Bonnet, Christophe Richard, Marjolaine André, Valérie Gelin, Marie-Christine Deloche, Aurore Lamadon, Gwendoline Morin, Béatrice Mandon-Pépin, Eugénie Canon, Dominique Thépot, Johann Laubier, Katayoun Moazami-Goudarzi, Ludivine Laffont, Olivier Dubois, Thierry Fassier, Patrice Congar, Olivier Lasserre, Tiphaine Aguirre-Lavin, Jean-Luc Vilotte, Eric Pailhoux

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Detection Methods for Asparagine and Glutamine Contents in Fruits and Vegetables and Recommendations for Dietary Intake of Fruits and Vegetables in Patients with Urea Cycle Disorde... by YE Xinyu, LI Ziying, TANG Shujun, SUN Liping, ZHAO Yunxia, XIAO Weimin, GU Yaping, KUANG Huiying, CHEN Xin, YANG Guowu, HUANG Yali

“…The calibration curves for Asn and Gln showed a good linearity in the range of 10–1 000 ng/mL (R2 > 0.999), with limit of detection (LOD) and limit of quantification (LOQ) of 1.5 and 5.0 mg/100 g, respectively. …”
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Effects of home-based virtual reality upper extremity rehabilitation in persons with chronic stroke: a randomized controlled trial by Hiroyuki Ase, Kaoru Honaga, Mami Tani, Tomokazu Takakura, Futoshi Wada, Yuhei Murakami, Reina Isayama, Akira Tanuma, Toshiyuki Fujiwara

“…Trial registration: This study was registered in the University Hospital Medical Information Network (UMIN) Clinical Trial Registry in Japan (Unique Identifier: UMIN000038469) on November 1, 2019; https://center6.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000043836 .…”
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Effects of a 16-week dance intervention on loneliness and self-esteem in left behind children: a randomised controlled trial by Xiaolin Li, Qian Yang, Jiayi Long, Linghui Zhou, Chunxia Lu, Zhenqian Zhou, Ming Zeng, Weixin Dong

“…(b) There was a significant negative correlation between loneliness and self-esteem among the LBC (p < 0.001, r = − 0.390). (c) After the dance intervention, the LBC’s loneliness significantly decreased [F (2, 1258) = 74.516, η p 2 = 0.106, p < 0.001] and their self-esteem significantly increased [F (2, 1258) = 73.64, η p 2 = 0.104, p < 0.001]. …”
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Characterization of serum metabolome and respiratory microbiota in children with influenza A virus infection by Xinyi Shi, Shenghao Hua, Zeyuan Chen, Weiyi Cao, Mengqing Xiao, Wenlong Pei, Zhe Cao, Zhan Zhang, Haibing Yang, Haibing Yang, Xuejun Shao, Yu Xia, Yu Xia

“…Ultra performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF/MS) and 16S rRNA gene sequencing were employed to analysis the respiratory microbiome and serum metabolic characteristics of 85 patients with IAV infection and age-matched 55 controls with respiratory disease who tested negative for 13 types of respiratory pathogens. …”
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The characteristics of chronic benzene poisoning in 176 Chinese occupational population cases by Lian Gou, Lian Gou, Xingyu Ma, Lili Huang, Lili Huang, Mei Qiu, Ruiqing Guo, Jun Jia, Peiyu Xu, Nan Lian

“…Linear regression analysis indicated that the number of workers increased with year as the independent variable (r2 = 0.40, p = 0.016). The working duration of benzene exposure appeared to decline, but this trend was not statistically significant. …”
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Mobilization of plant genetic resources from the territory of India by N. P. Loskutova, T. M. Ozerskaya

“…In 1977, another collecting team led by R. A. Udachin, whose task was to study the system of nation-wide projects on crop improvement, traveled over five states, visited 24 scientific and academic institutions, and collected 3189 germplasm samples. …”
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Detección del polimorfismo T991T en familias cubanas con diagnóstico clínico de la enfermedad de Wilson by Yulia Clark Feoktistova, Caridad Ruenes Domech, Elsa F. García Bacallao, Teresa Collazo Mesa, Hilda Roblejo Balbuena, Estela Morales Peralta, Iliana Clark Feoktistova, Zoe Robaina Jiménez

“…En el 71,4 % de los pacientes que presentaron el polimorfismo T991T se identificó además el polimorfismo K832R. En un paciente se identificó la mutación L708P.…”
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The analysis of the effect of piston speed on the operation of a refrigeration compressor operating in the area of wet steam by D. Kh. Sadvakasov, G. I. Chernov, V. S. Evdokimov, N. A. Raykovskiy

“…The paper presents a theoretical assessment of the effect of the piston speed on the energy efficiency of a steam compression refrigeration cycle based on compression in the area of wet steam, implemented in a low-speed refrigeration compressor stage. The refrigerant R134a was chosen as the working fluid. The numerical calculation of the working process of a refrigeration compressor operating in the field of wet steam was carried out on the basis of a mathematical model and implemented using the Runge– Kutta method of the 4th order in the MathCad package. …”
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Aerobic capacity and muscle proteome: Insights from a mouse model by Abel Plaza‐Florido, Alejandro Santos‐Lozano, Susana López‐Ortiz, Beatriz G. Gálvez, Joaquín Arenas, Miguel A. Martín, Pedro L. Valenzuela, Tomàs Pinós, Alejandro Lucia, Carmen Fiuza‐Luces

“…AC was significantly associated (P‐values ranging from 0.0002 to 0.049) with 73 (McArdle) and 61 (wild‐type) proteins (r‐values from −0.90 to 0.94). These proteins were connected in PPI networks that enriched biological processes involved in skeletal muscle structure/function in both groups (false discovery rate <0.05). …”
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Prevalence and investigation of Cytomegalovirus (HCMV) in blood donors from the main blood establishment in Rio de Janeiro/Brazil by Agildo da Silva Oliveira, Jéssica Gonçalves Pereira, Gabrielle Tantos Nunes, Ivanildo Pedro de Sousa Junior, Dmitry José de Santana Sarmento, Josiane Iole França Lopes, Luiz Amorim Filho, Vanessa Salete de Paula

“…The risk persists even after prophylaxis ends, especially in patients who undergo organ transplantation and receive blood or blood products from a seropositive donor while being seronegative themselves (D+/R-). Materials and methods: Here, we investigated the serological and molecular prevalence of HCMV among 980 blood donors from the main blood bank in Rio de Janeiro, Brazil, using chemiluminescence and real-time PCR (TaqMan). …”
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Evaluation of chicken liver meal as fish meal alternative in large yellow croaker Larimichthys crocea diet: Effects of growth performance, antioxidant capacity, intestinal health a... by Jianchun Shao, Yujie Wang, Liwen Wu, Qingyin Zheng, Wenbo Zhu, Zhengbang Chen, Dianguang Zhang

“…The CLM75 and CLM100 diets led to a significant decrease in the activity of amylase (AMS), lipase (LPS), catalase (CAT) as well as total antioxidant capacity (T-AOC) for liver of Larimichthys crocea, while remarkably increasing malondialdehyde (MDA) content. 16S rRNA sequencing revealed that CLM75 and CLM100 diets resulted in increased relative abundances of Proteobacteria phylum and decreased relative abundances of Firmicutes phylum. …”
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The extensive cultivation of Pyropia haitanensis along the coastal areas influences the ecological dynamics of nearby intertidal mudflat pond water by Peihao Liu, Qijun Luo, Shanshan Zhu, Juanjuan Chen, Rui Yang, Tingting Niu, Tiegan Wang, Peng Zhang, Haimin Chen

“…The dynamic diversity patterns and structure of microbial communities were determined by 16S and 18S rRNA sequencing, and the environmental factors were measured.ResultsIn the offshore aquaculture area, the cultivation of P. haitanensis increased the dissolved oxygen and pH, decreased inorganic nutrients, reduced the alpha diversity of bacteria, established the dominance of Proteobacteria, but did not influence the composition of microeukaryotes. …”
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Causal machine learning models for predicting low birth weight in midwife-led continuity care intervention in North Shoa Zone, Ethiopia by Wudneh Ketema Moges, Awoke Seyoum Tegegne, Aweke A. Mitku, Esubalew Tesfahun, Solomon Hailemeskel

“…Conclusions Based on these findings, the CKNN classifier demonstrated a higher accuracy and effectiveness. The S-learner and R-learner models, utilizing the XGBoost Regressor and BaseSRegressor, provided accurate estimations of ITE for assessing the impact of the MLCC program. …”
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