Performance evaluation of nine reference centers and comparison of DNA extraction protocols for effective surveillance of Leishmania-infected Phlebotomine sand flies: Basis for tec... by Jorian Prudhomme, Aymeric Delabarre, Bulent Alten, Umut Berberoglu, Eduardo Berriatua, Gioia Bongiorno, José Manuel Cristovao, Maya Davidovich-Cohen, Trentina Di Muccio, Ozge Erisoz Kasap, Eleonora Fiorentino, Oscar D Kirstein, Edwin Kniha, Carla Maia, Mesut Mungan, Clara Muñoz-Hernández, Muhammed Nalçaci, Gizem Oguz Kaskan, Yusuf Ozbel, Seray Ozensoy Toz, Ricardo Parreira, Katharina Platzgummer, Ceylan Polat, José Risueño, Liora Studentsky, Gamze Varol, Julia Walochnik, Kardelen Yetişmiş, Florence Robert-Gangneux

“…Consistent quantitative real-time PCR (qPCR) parameters and efficient DNA extraction protocols are crucial for reliable results over time and across regions. …”
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Efficacy of transcranial magnetic stimulation in anorexia nervosa: a systematic review and meta-analysis by Amir Reza Bahadori, Parisa Javadnia, Sanaz Bordbar, Rasa Zafari, Tina Taherkhani, Afshan Davari, Abbas Tafakhori, Sajad Shafiee, Sara Ranji

“…The analysis exhibited a significant decrease in EDE-Q score after TMS (SMD: 0.634, 95% CI: 0.349–0.919, P-value < 0.001). …”
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Unraveling the transcriptomic effects of leucine supplementation on muscle growth and performance in basketball athletes. by Sinan Wang, Weishuai Guo, Rui Dong

“…WGCNA highlighted a key module associated with muscle growth, enriched in cytoskeletal pathways. qPCR validation confirmed the upregulation of cytoskeleton-related genes, supporting the transcriptomic findings. …”
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The relationships among food neophobia, mediterranean diet adherence, and eating disorder risk among university students: a cross-sectional study by Nilufer Ozkan, Fatma Hazan Gul

“…A positive correlation was found between age and KIDMED adherence, whereas a negative correlation was observed between BMI and EDE-Q scores regarding food neophobia. The effects of BMI on food neophobia and the risk of eating disorders were found to vary by sex. …”
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Human and entomological determinants of malaria transmission in the Lihir Islands of Papua New Guinea: A cross-sectional study. by Pere Millat-Martínez, Michelle Katusele, Bernadine Kasian, Elias Omera, Esther Jamea, Lina Lorry, Aina Casellas, Dan Ouchi, Chilaka Wali, Sylvia Raulo, Arthur Elizah, Peter Kaman, Absalom Dau, Muker Sakur, Lemen Kilepak, Siub Yabu, Nelson Koata, John Kave, Michael Toa, Christopher Urakusie, Charles Kongs, Frank Kisba, Moses Laman, Oriol Mitjà, William Pomat, Quique Bassat, Stephan Karl, Bàrbara Baro

“…Prevalence (qPCR) varied across geographic areas, from 8.5% in the mine-impacted zone (MIZ) to 27.0% in the non-MIZ. …”
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Higher systemic immune-inflammation index is associated with increased risk of Parkinson’s disease in adults: a nationwide population-based study by Jiayu Zhao, Zhipeng Wu, Zhipeng Wu, Fengyin Cai, Xuejv Yu, Zhenyu Song

“…The results of logistic regression showed that after adjusted for all covariates, compared with the Q1 group (lowest quartile in SII), the risk of PD in the Q3 group (OR = 1.82, 95%CI = 1.20–2.82, p &lt; 0.001) and the Q4 group increased (OR = 2.49, 95%CI = 1.69–3.77, p &lt; 0.001), with p-trend &lt; 0.001. …”
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Dose‐exposure‐response of CARDALIS® (benazepril/spironolactone) on the classical and alternative arms of the renin‐angiotensin‐aldosterone system in healthy dogs by Elizabeth Manson, Jessica L. Ward, Maria Merodio, Emilie Guillot, Thomas Blondel, Karin Allenspach, Oliver Domenig, Jonathan P. Mochel

“…Methods Three groups of 6 dogs received different doses of CARDALIS® for 14 days following induction of RAAS activation by feeding a low‐sodium diet: (a) benazepril 0.25 mg/kg + spironolactone 2 mg/kg PO q24h (label dose); (b) benazepril 0.25 mg/kg + spironolactone 2 mg/kg PO q12h; or (c) benazepril 0.5 mg/kg + spironolactone 4 mg/kg PO q12h. …”
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Continuous-flow columns packed with zero-valent iron and iron sulfide as a feasible strategy to remediate the persistent contaminant nitroguanidine by Youngjae Yu, Erika E. Rios-Valenciana, Robert A. Root, Reyes Sierra-Alvarez, Jon Chorover, Jim A. Field

“…Both reactors transformed NQ into nitrosoguanidine (NsoQ). Further transformation of NsoQ by ZVI produced aminoguanidine, guanidine, and cyanamide, whereas NsoQ transformation by FeS produced guanidine, ammonium, and traces of urea. …”
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Surgical planning of small intestine neuroendocrine tumors: the concept of mesenteric tumor deposits by Romain L’Huillier, Gilles Poncet, Arnaud Pasquer, Thomas Walter, Catherine Lombard-Bohas, Valérie Hervieu, Bénédicte Cayot, Pierre-Jean Valette, Helen Cheung, Laurent Milot

“…The negative predictive value of CT was high (97% (34/35) to 100% (35/35)) for duodenal resection (ĸ = 0.78). Regarding retropancreatic lymph node invasion, the CT sensitivity was poor (24%, 4/17), with a high ĸ (0.88). …”
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The Novel Methods for Analysis of Exosomes Released from Endothelial Cells and Endothelial Progenitor Cells by Jinju Wang, Runmin Guo, Yi Yang, Bradley Jacobs, Suhong Chen, Ifeanyi Iwuchukwu, Kenneth J. Gaines, Yanfang Chen, Richard Simman, Guiyuan Lv, Keng Wu, Ji C. Bihl

“…Here we described novel methods for purification and phenotyping of EXs released from endothelial cells (ECs) and endothelial progenitor cells (EPCs) by combining microbeads and fluorescence quantum dots (Q-dots®) techniques. EXs from the culture medium of ECs and EPCs were isolated and detected with cell-specific antibody conjugated microbeads and second antibody conjugated Q-dots by using nanoparticle tracking analysis (NTA) system. …”
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A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways by Duo Wang, Gangli Ma, Jia Shen, Xinyang Xu, Weisong Shou, Zhengying Xuan, Yanjun He

“…Quantitative real-time PCR (qRT-PCR) analysis indicated that <i>ClSAUR1</i> is ubiquitously expressed across various watermelon tissues, with pronounced expression in the roots and leaves. …”
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Phosphorus Fertilization and Chemical Root Pruning: Effects on Root Traits During the Nursery Stage in Two Mediterranean Species from Central Chile by Fiorella Calderón-Ureña, Carolina Álvarez-Maldini, Manuel Acevedo, Manuel E. Sánchez-Olate, R. Kasten Dumroese, Antay Sierra-Olea, Juan F. Ovalle, Edwin Esquivel-Segura

“…The root-to-shoot ratio declined with WCu in <i>A. chilensis</i> (23.1%) and <i>Q. saponaria</i> (15.7%). Unlike our hypothesis, fine root architecture remained unaffected with root pruning in <i>A. chilensis</i>, while fine root length and volume decreased with increasing P concentrations in <i>Q. saponaria</i>. …”
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Development and application of reverse transcriptase droplet digital PCR technology for sensitive detection of BVDV-1 and BTV in bovine semen by Zhichao Yu, Linjun Chen, Qiang Cui, Han Yan, Junyan Li, Xiaoping Luo, Yingying Li, Xianghong Ju, Yanhong Yong, Namula Zhao, Zhiguo Zhao

“…The results revealed that the positivity rates of BVDV-1 and BTV RT‒ddPCR (25% and 23%, respectively) were greater than those of RT‒qPCR (19% and 18%, respectively). Conclusions RT‒ddPCR was highly sensitive for detecting low concentrations of BVDV-1 and BTV in clinical samples and could be a good supplement for qPCR testing.…”
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CRISPR/Cas13a combined with reverse transcription and RPA for NoV GII.4 monitoring in water environments by Yiqiang Sun, Weiwei Zhang, Houyun Zhang, Feng Zhao, Laijin Su

“…Four water samples, totaling 64 samples, were analyzed using this method and compared with the qPCR method. The results of the two methods showed 100 % concordance with no significant difference in viral load. …”
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miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia by Mete Ozkurt, Thomas Hellwig-Bürgel, Reinhard Depping, Selda Kadabere, Rumeysa Ozyurt, Abdullah Karadag, Nilüfer Erkasap

“…First, we determined the dose of TNF-α in HepG2 cells that has no cytotoxic effect by using MTT assays and that inhibits Epo synthesis by qRT-PCR and ELISA. Then, we performed the microRNA array study with TNF-α (20 ng/ml)-treated cells, and the array results were confirmed by qRT-PCR. …”
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Donor Identification, Genetic Diversity, Population Structure and Marker–Trait Association Analyses for Iron Toxicity Tolerance Using Rice Landraces by Debanjana Saha, Udit Nandan Mishra, Chittaranjan Sahoo, Seema Tripathy, Uttam Kumar Behera, Susmita Das, Chandrasekhar Sahu, Shiv Datt, Manoj Kumar Rout, Tanmaya Lalitendu Mohanty, Shakti Prakash Mohanty, Saumya Ranjan Barik, Ishwar Chandra Mohanty, Sharat Kumar Pradhan

“…Marker–trait association analysis validated the reported iron toxicity tolerance QTLs <i>qFeTox 4.2</i> and <i>qFeTox 4.3</i>, which are useful for marker-assisted improvement. …”
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Associations of fasting plasma glucose and glycosylated hemoglobin levels at less than 24 weeks of gestation with hypertensive disorders of pregnancy: the BOSHI study by Seiya Izumi, Noriyuki Iwama, Hirotaka Hamada, Taku Obara, Mami Ishikuro, Michihiro Satoh, Takahisa Murakami, Masatoshi Saito, Takayoshi Ohkubo, Takashi Sugiyama, Shinichi Kuriyama, Nobuo Yaegashi, Kazuhiko Hoshi, Yutaka Imai, Hirohito Metoki, the BOSHI Study Group

“…The odds ratios (ORs) were 1.334 (95% confidence interval [CI]: 1.002–1.775) for Q4 of FPG and 1.405 (95% CI: 1.051–1.878) for Q4 of HbA1c. …”
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Single-cell profiling reveals a reduced epithelial defense system, decreased immune responses and the immune regulatory roles of different fibroblast subpopulations in chronic atro... by Lin Lin, Tingxuan Huang, Lizhi Li, Yang Lin, Feng Chen, Ziyi Zheng, Jie Zhou, Yizhe Wang, Weihao You, Yujie Duan, Yawen An, Shiwei He, Weimin Ye

“…Metabolic analysis was performed to compare the metabolic activity of C1Q + macrophages under different conditions. NichNet analysis was used to analyze the regulatory relationships between CCL11 + APOE + fibroblasts and C1Q + macrophages and between CCL11 + APOE + fibroblasts and CD8 + effector T cells. …”
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Scrutinizing $$B^0$$ B 0 -meson flavor changing neutral current decay into scalar $$K_0^*(1430)$$ K 0 ∗ ( 1430 ) meson with $$b\rightarrow s \ell ^+\ell ^-(\nu \bar{\nu })$$ b → s... by Yin-Long Yang, Ya-Xiong Wang, Hai-Bing Fu, Tao Zhong, Ya-Lin Song

“…Meanwhile, we extrapolate TFFs to the whole physical $$q^2$$ q 2 -region by using the simplified $$z(q^2)$$ z ( q 2 ) -series expansion. …”
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Characterization of RAP Signal Patterns, Temporal Relationships, and Artifact Profiles Derived from Intracranial Pressure Sensors in Acute Traumatic Neural Injury by Abrar Islam, Amanjyot Singh Sainbhi, Kevin Y. Stein, Nuray Vakitbilir, Alwyn Gomez, Noah Silvaggio, Tobias Bergmann, Mansoor Hayat, Logan Froese, Frederick A. Zeiler

“…To identify artifacts, (a) ICP <i>q</i>-order, AMP <i>p</i>-order, and RAP <i>p</i>-order and <i>q</i>-order, (b) residuals of ICP, AMP, and RAP, and (c) cross-correlation between residuals of RAP and AMP proved to be useful at the minute-by-minute resolution, whereas, for the 10-min-by-10-min data resolution, only the <i>q</i>-order of the optimal ARIMA model of ICP and AMP served as a distinguishing factor. …”
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