-
4481
-
4482
ZC3H15 suppression ameliorates bone cancer pain through inhibiting neuronal oxidative stress and microglial inflammation
Published 2025-03-01“…Methods: To evaluate the expression of ZC3H15 in cancer-induced pain, we used microcomputed tomography (MicroCT), immunoblotting, co-immunoprecipitation (Co-IP), behavior tests, quantitative real-time polymerase chain reaction (qRT-PCR), and immunofluorescence assays in this investigation. …”
Get full text
Article -
4483
The Interactive Effects of Fruit Intake Frequency and Serum miR-484 Levels as Biomarkers for Incident Type 2 Diabetes in a Prospective Cohort of the Spanish Adult Population: The D...
Published 2025-01-01“…Baseline serum miR-484 levels were measured using real-time qPCR and categorized based on the 25th percentile. …”
Get full text
Article -
4484
Myocarditis and neutrophil-mediated vascular leakage but not cytokine storm associated with fatal murine leptospirosisResearch in context
Published 2025-02-01“…Bacterial load, biochemical parameters, cytokine production and leucocyte population were assessed by qPCR, ELISA, cytometry and immunohistochemistry. …”
Get full text
Article -
4485
Exosome‐derived long non‐coding RNA AC010789.1 modified by FTO and hnRNPA2B1 accelerates growth of hair follicle stem cells against androgen alopecia by activating S100A8/Wnt/β‐cat...
Published 2025-01-01“…Methods The expression and prognosis of lncRNA AC010789.1 in AGA hair follicle tissues were assessed by qRT‐PCR analysis. CCK‐8, EdU and Transwell analysis were utilized to assess cell growth. …”
Get full text
Article -
4486
MicroRNAs Expression Profile in MN1-Altered Astroblastoma
Published 2025-01-01“…MiRNA expression profiles were then detected on <i>MN1</i>-altered astroblastomas (<i>n</i> = 8) and normal brain controls (<i>n</i> = 2) by Nanostring technology and validated by RT-qPCR; then, the expression of deregulated miRNAs was correlated with clinical-pathological characteristics. …”
Get full text
Article -
4487
Iodine activates NLRP3 inflammasomes in PBMCs of patients with autoimmune thyroiditis and regulates Th1 and Th17 cell differentiation
Published 2025-01-01“…., NLRP1, NLRP3, absent in melanoma 2 (AIM2) and caspase-1), interleukin (IL)-1β and IL-18 were assessed using qRT-PCR and western blotting. Enzyme-linked immunosorbent assays (ELISAs) assessed the serum levels of IL-1β and IL-18. …”
Get full text
Article -
4488
The diagnostic performance of International Ovarian Tumor Analysis: Simple Rules for diagnosing ovarian tumors—a systematic review and meta-analysis
Published 2025-01-01“…After estimating the pooled effect of the sensitivity, specificity, and diagnostic odds ratio (DOR), the summary receiver operating characteristic (SROC) curve was estimated using the bivariate random effects model. Utilizing Cochran’s Q statistics and Higgins’s inconsistency test through the I2 index for pooled analysis, the heterogeneity of studies was quantitatively evaluated. …”
Get full text
Article -
4489
Combined Effects of Humic Acid-Modified Magnetic Biochar and AMF on the Properties of Saline-Alkali Soil and Photosynthetic Parameters of Muskmelon
Published 2024-12-01“…On one hand, the iron oxides and humic acids loaded on HA-MBC improved the physical and chemical properties of soil by increasing the quantity of surface functional groups and porosity, while AMF formed symbiotic roots to further enrich organic matter and nutrients, and increase soil enzyme activities, thus increasing the height and root length of muskmelon and promoting the recovery of Fv/Fm and the reduction of F0 and qN. On the other hand, the application of HA-MBC could increase the colonization rate of AMF in saline and alkaline soils by 19.46% and 22.72%, respectively, thus further enhancing the effects of AMF. …”
Get full text
Article -
4490
PCSK9-antibodies fail to block PCSK9-induced inflammation in macrophages and cannot recapitulate protective effects of PCSK9-deficiency in experimental myocardial infarction
Published 2025-01-01“…PCSK9 and LDL receptor levels were measured using ELISA and qRT-PCR. Cardiac function was assessed via echocardiography and isolated working heart model experiments. …”
Get full text
Article -
4491
Compositional Design, Microstructure, and Thermal Processing of Aluminum-Based Complex Concentrated Alloys
Published 2025-01-01“…Alloys A and AM were characterized by a multiphase microstructure with a dendritic FCC-Al matrix phase and various secondary phases (Q-AlCuMgSi, Al<sub>2</sub>Cu and Mg<sub>2</sub>Si), while alloy B consisted of a parent phase T-Mg<sub>32</sub>(Al,Zn)<sub>49</sub> and the secondary phases α-Al and Mg<sub>2</sub>Si. …”
Get full text
Article -
4492
SARS-CoV-2 excretion and genetic evolution in nasopharyngeal and stool samples from primary immunodeficiency and immunocompetent pediatric patients
Published 2025-01-01“…RNA detection was performed using RT-qPCR, and whole-genome sequencing was conducted with the NexSeq 1000 platform. …”
Get full text
Article -
4493
Polygonatum kingianum polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiota
Published 2025-01-01“…There were 5 phyla and 20genera of remarkable bacteria found among mice groups including genera of Escherichia, Pseudomonas_E, Mailhella, Paramuribaculum, NM07-P-09, Odoribacter, Nanosyncoccus, SFM01, Onthenecus, Clostridium_Q, UBA6985, Ructibacterium, UBA946, Lachnoclostridium_B, Evtepia, CAG-269, Limivicinus, Formimonas, Dehalobacterium, Dwaynesavagella, and UBA6985. …”
Get full text
Article -
4494
Phenomenology of Organizational Silence (Case Study Farhangian University)
Published 2024-09-01“…Data analysis was conducted using Smith's 6-step content analysis and coding process with Max.Q.DA software. Results The interview analysis results were organized into three main categories: external organizational, internal organizational, and individual, with 11 main themes and 64 subthemes. …”
Get full text
Article -
4495
Gut bacteria Prevotellaceae related lithocholic acid metabolism promotes colonic inflammation
Published 2025-01-01“…Histological analysis, quantitative PCR (qPCR) and Western Blotting were conducted on colonic samples from DSS-induced colitis mice to assess colonic inflammation, mucosal barrier integrity, and associated signaling pathways. …”
Get full text
Article -
4496
Lentivirus‐mediated gene therapy for Fabry disease: 5‐year End‐of‐Study results from the Canadian FACTs trial
Published 2025-01-01“…Globotriaosylceramide (Gb3) and lyso‐Gb3 levels in urine and plasma were assessed by mass spectrometry. qPCR assays measured vector copy number in PB leucocytes. …”
Get full text
Article -
4497
The relationship between stress levels and the habits of consuming high-sugar food among undergraduate students
Published 2025-01-01“…Respondent characteristics were collected using a structured questionnaire, high-sugar food consumption habits were assessed using the Q-FFQ, and stress levels were measured with the DASS questionnaire. …”
Get full text
Article -
4498
Viral load suppression rate of third-line antiretroviral therapy and its association with gender among HIV patients after second-line treatment failure in Africa: a systematic revi...
Published 2025-02-01“…Heterogeneity was evaluated with Cochrane’s Q and I2 statistics. Publication bias was checked using a funnel plot, Begg’s and Egger’s test, followed by the DerSimonian and Laird random effects model. …”
Get full text
Article -
4499
Effect of the gut core microbiota Cetobacterium on the growth, physiology, and nutritional metabolism of Nile tilapia (Oreochromis niloticus)
Published 2025-03-01“…Furthermore, analysis of the liver transcriptome and RT-qPCR data indicated a decrease of genes expression of fatty acid synthesis (SREB 1c, ACCα, and FAS) with NK01 supplementation feeding group. …”
Get full text
Article -
4500
RECQL4 affects MHC class II‐mediated signalling and favours an immune‐evasive signature that limits response to immune checkpoint inhibitor therapy in patients with malignant melan...
Published 2025-01-01“…Abstract Background Cancer immunotherapy has transformed metastatic cancer treatment, yet challenges persist regarding therapeutic efficacy. RECQL4, a RecQ‐like helicase, plays a central role in DNA replication and repair as part of the DNA damage response, a pathway implicated in enhancing efficacy of immune checkpoint inhibitor (ICI) therapies. …”
Get full text
Article