Attitudes Toward Psychotherapeutic Treatment and Health Literacy in a Large Sample of the General Population in Germany: Cross-Sectional Study by Rebekka Schröder, Tim Hamer, Ralf Suhr, Lars König

“…Attitudes toward psychotherapy and both general and mental health literacy were assessed using the Questionnaire on Attitudes Towards Psychotherapeutic Treatment (QAPT) with 2 subscales (“positive attitudes” and “non-acceptance of society”), the European Health Literacy Survey instrument (HLS-EU-Q16) and the Mental Health Literacy Tool for the Workplace (MHL-W-G). …”
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Effects of Diets Containing Extruded Linseed and <i>Padina pavonica</i> Algae on Meat Rabbit: Carcass Performance and Meat Quality by Nour Elhouda Fehri, Michela Contò, Marta Castrica, Alda Quattrone, Gianluca Renzi, Sabrina Di Giovanni, Stella Agradi, Daniele Vigo, Gabriele Brecchia, Laura Menchetti, Claudia Maria Balzaretti, Doriana Beqiraj, Egon Andoni, Giulio Curone, Sebastiana Failla

“…Meat analyses were performed on <i>Longissimus thoracis</i> et <i>lumborum</i> (LTL) muscle for physical properties and on thigh meat (THM) for proximate composition, vitamin E, coenzyme-Q10, cholesterol, fatty acid profile, and mineral content. …”
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The Effect of Vascular Endothelial Growth Factor on Bone Marrow Mesenchymal Stem Cell Engraftment in Rat Fibrotic Liver upon Transplantation by Ke Yuan, Chunyou Lai, Lingling Wei, Tianhang Feng, Qinyan Yang, Tianying Zhang, Tao Lan, Yutong Yao, Guangming Xiang, Xiaolun Huang

“…Paraffin section staining, immunofluorescent staining, RT-qPCR (quantitative reverse transcription polymerase chain reaction), and Western blot were used to evaluate hepatic pathological changes and physiology function. …”
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lncRNA-SNHG14 Plays a Role in Acute Lung Injury Induced by Lipopolysaccharide through Regulating Autophagy via miR-223-3p/Foxo3a by Jun Hong, Shijing Mo, Fangxiao Gong, Zongbin Lin, Hanhui Cai, Ziqiang Shao, Xianghong Yang, Renhua Sun, Qiangnu Zhang, Jingquan Liu

“…The expression changes of lncRNA-SNHG14/miR-223-3p/Foxo3a were confirmed using qRT-PCR and west blot. The binding relationship of lncRNA-SNHG14/miR-223-3p/and miR-223-3p/Foxo3a was verified using dual-luciferase reporter, RNA immunoprecipitation, and RNA pull-down assays. …”
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Overexpression of OsDUF868.12 enhances salt tolerance in rice by Hao Chen, Jiale Wan, Jiali Zhu, Ziyi Wang, Caiyao Mao, Wanjing Xu, Juan Yang, Yijuan Kong, Xiaofei Zan, Rongjun Chen, Jianqing Zhu, Zhengjun Xu, Lihua Li, Lihua Li

“…A series of experiments, including RT-qPCR, Agrobacterium-mediated transient transformation in tobacco for localization analysis, phenotypic characterization, physiological and biochemical index measurement, and leaf staining, were conducted to investigate the function of OsDUF868.12 under salt stress. …”
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Reduced Glutathione Promoted Growth Performance by Improving the Jejunal Barrier, Antioxidant Function, and Altering Proteomics of Weaned Piglets by Zhimei Tian, Yiyan Cui, Miao Yu, Dun Deng, Zhenming Li, Xianyong Ma, Mingren Qu

“…The results of proteomics and qRT-PCR showed that GSH supplementation might dose-dependently promote growth performance and that it alleviated the weaning stress-induced oxidative injury of the jejunal mucosa in piglets by activating SIRTI and Akt pathways to regulate GPX4, HSP70, FoxO1. …”
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Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike Protein by Wang BH, Yu KY, Zhang XN, Sun XH, Tang LL, Shi XL

“…Observe the pathological changes in lung tissues, expression of inflammatory factors, and mucins in different groups of mice using HE and PAS staining methods, as well as ELISA and RT-qPCR. Flow cytometry was used to detect T helper 17 (Th17)/regulatory T (Treg) cells and T helper 1(Th1)/T helper 2 (Th2) lymphocyte ratios and the proportions of conventional myeloid dendritic cells (cDCs), plasma cell-like DCs, CD80 and CD86 cells in mouse spleens.Results: HE and PAS staining showed that, compared to that in the normal group, the lung tissue of the D1 group mice showed a significant inflammatory damage response, whereas the D3 and D10 groups showed a gradual recovery trend. …”
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NALCN expression is down-regulated and associated with immune infiltration in gastric cancer by Xuewei Li, Xuewei Li, Na Wu, Chen Wang, Beibei Pei, Xiaoyan Ma, Jun Xie, Jun Xie, Wenhui Yang

“…However, there is a paucity of experimental studies specifically addressing the relationship between NALCN and immune cell infiltration in gastric cancer (GC).MethodsThe expression levels of NALCN in tumor tissues, peripheral blood and gastric cancer cells lines from patients with GC were assessed using RNA sequencing, immunohistochemistry (IHC) staining and RT-qPCR. Data obtained from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases were utilized to investigate the correlation between NALCN expression and immune cell infiltration in GC. …”
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Quinazolinone Derivative MR2938 Protects DSS-Induced Barrier Dysfunction in Mice Through Regulating Gut Microbiota by Ling Lv, Mireguli Maimaitiming, Jichen Yang, Shuli Xia, Xin Li, Pingyuan Wang, Zhiqing Liu, Chang-Yun Wang

“…The inflammatory factor levels were performed by quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA). …”
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High fructose rewires gut glucose sensing via glucagon-like peptide 2 to impair metabolic regulation in mice by Eya Sellami, Paulo Henrique Evangelista-Silva, Caio Jordão Teixeira, Khoudia Diop, Patricia Mitchell, Fernando Forato Anhê

“…Glucose tolerance, insulin sensitivity, and gut glucose absorption were concomitantly assessed, and enteric sugar transporters and absorptive surface were quantified by RT-qPCR and histological analysis, respectively. Results: High fructose feeding led to impairment of blood glucose disposal, ectopic fat accumulation in the liver, and hepatic (but not muscle or adipose tissue) insulin resistance independent of changes in fat mass. …”
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miR‐130a‐3p regulated TGF‐β1‐induced epithelial‐mesenchymal transition depends on SMAD4 in EC‐1 cells by Xiaokang Tian, Qian Fei, Mingyu Du, Hongming Zhu, Jinjun Ye, Luxi Qian, Zhiwei Lu, Wenjun Zhang, Yan Wang, Fanyu Peng, Jie Chen, Baoling Liu, Qian Li, Xia He, Li Yin

“…The expression of miR‐130a‐3p in ESCC cell line and normal esophageal epithelial cell was determined by RT‐qPCR. The protein expression levels of TGF‐β‐induced changes in EMT were analyzed by western blotting and immunofluorescence. …”
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JAK/STAT3 signaling promotes pain and depression-like behaviors in rats with bone cancer pain by regulating Th17 cell differentiation by Shuyan Wu, Jundan Jiang, Danfeng Wang, Daoyi Lin, Mingxue Lin, Pinzhong Chen, Jianghu Chen, Honghong Zhang, Ying Wang, Xiaohui Chen, Xiaochun Zheng

“…Changes in inflammatory factors and related protein expression levels in the amygdala were detected using western blotting, immunofluorescence, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The effects of intra-amygdala injections of a lentivirus targeting retinoic acid-related orphan receptor γt (RORγt) (LV-shRORγt), nifuroxazide (a STAT3 antagonist), and colivelin (a STAT3 agonist) were evaluated. …”
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Causal role of plasma liposome in diabetic retinopathy: mendelian randomization (MR) study by Kai Yin, Lu Ding, Xueyan Li, Yuqi Zhang, Siyu Song, Liyuan Cao, Ruixue Deng, Min Li, Zirui Li, Qinjing Xia, Daqing Zhao, Xiangyan Li, Zeyu Wang

“…Heterogeneity was checked using Cochran’s Q statistic. The MR Pleiotropy Residual Sum and Outlier (MR-PRESSO) global test and the MR-Egger regression were used to detect horizontal pleiotropy. …”
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Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications by Enrico Ragni, Andrea Papait, Michela Maria Taiana, Paola De Luca, Giulio Grieco, Elsa Vertua, Pietro Romele, Cecilia Colombo, Antonietta Rosa Silini, Ornella Parolini, Laura de Girolamo

“…Methods: Immunophenotype and secretory ability at soluble protein and EV-related levels, including embedded miRNAs, were analysed in the secretomes by means of flow cytometry, nanoparticle tracking analysis, high throughput ELISA and qRT-PCR arrays. Secretomes effect was tested in in vitro models of chondrocytes, lymphocytes and monocytes to mimic the OA microenvironment. …”
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O debate entre cosmopolitismo, patriotismo e nacionalismo: uma introdução geral ao tema by Mariane Gehlen Perin

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Social learning activities to improve community engagement in waste management program by S. Sunarti, R.S.Y. Zebua, J.H. Tjakraatmadja, A. Ghazali, B. Rahardyan, K. Koeswinarno, S. Suradi, N. Nurhayu, R.H.A. Ansyah

“…Instead, path model analysis indicated the mediating effect  of Practice-based learning for Dialogue-based learning and Affective Factors, with the accuracy model at a moderate level (R2 = 42%; Q2 = 0.2258). Meanwhile, supporting facilities influenced both Practice-based learning (β = 0.3116, P < 0.001) and Affective factors (β = 0.4419, P < 0.001) significantly. …”
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Platinum drugs upregulate CXCR4 and PD‐L1 expression via ROS‐dependent pathways, with implications for novel combined treatment in gastric cancer by Xiaoyu Kang, Lin Zhang, Shushang Liu, Fei Wang, Haiming Liu, Fenli Zhou, Fei Wu, Haohao Zhang, Daiming Fan, Yongzhan Nie, Zhangqian Chen

“…Immunohistochemistry, western blot, and RT‐qPCR were performed to determine the expression pattern of CXCR4 and PD‐L1 in GC. …”
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Arginase-1-specific T cells target and modulate tumor-associated macrophages by Mads Hald Andersen, Inés Lecoq, Ayako Wakatsuki Pedersen, Evelina Martinenaite, Shamaila Munir Ahmad, Maria Perez-Penco, Lucia Lara de la Torre, Marion Chapellier, Lars Rønn Olsen, Mia Aaboe-Jørgensen, Hannah Jorinde Glöckner, Anne Mette Askehøj Rømer

“…T-cell-mediated changes in the macrophage immune phenotype and cytokine microenvironment were examined using flow cytometry, RT-qPCR and multiplex immunoassay. The effect of Arg1-derived peptide IMV on TAMs in vivo was assessed by multiplex gene analysis of F4/80+cells.Results We show that Arg1-based IMV-mediated tumor control was linked to a decrease in multiple immunosuppressive pathways in the TAM population of the treated animals. …”
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Association between insulin resistance indices and outcomes in patients with heart failure with preserved ejection fraction by Weicheng Ni, Ruihao Jiang, Di Xu, Jianhan Zhu, Jing Chen, Yuanzhen Lin, Hao Zhou

“…When categorized into quartiles, the highest quartile of the TyG index (Q4) was significantly associated with MACE (HR 2.48, 95% CI 2.24–2.76, P < 0.001 in model 3). …”
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Green Tea Extract (<i>Theaceae</i>; <i>Camellia sinensis</i> L.): A Promising Antimicrobial, Anti-Quorum Sensing and Antibiofilm Candidate Against Multidrug-Resistant <i>Campylobac... by Mona S. Emara, Ahmed M. Ammar, Ashraf M.O. Abdelwahab, Attia A. Elgdawy, Adel Abdelkhalek, Elena Pet, Gabi Dumitrescu, Mirela Ahmadi, Norhan K. Abd El-Aziz

“…Furthermore, real-time quantitative polymerase chain reaction (RT-qPCR) results showed a significant decrease (<i>p</i> < 0.05) in the expression levels of biofilm biosynthesis gene and its regulator (<i>FlaA</i> and <i>LuxS</i>, respectively) in <i>Campylobacter</i> isolates treated with the green tea extract in comparison with untreated ones. …”
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