Caloric labels do not influence taste pleasantness and neural responses to erythritol and sucrose by Aleksandra Budzinska, Laura Byl, Fabienne Teysseire, Emilie Flad, Patrick Dupont, Bettina Wölnerhanssen, Anne Christin Meyer-Gerspach, Lukas Van Oudenhove, Nathalie Weltens

“…General Linear Model (GLM) analysis of brain responses at FDR q < 0.05 showed no main effect of sweetener nor label, nor a label-by-sweetener interaction. …”
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Resolution of oncogene-induced senescence markers in HPV-infected cervical cancer tissue by Ashraf I. Khasawneh, Sofian Al Shboul, Nisreen Himsawi, Amani Al Rousan, Nisreen Abu Shahin, Mohammed El-Sadoni, Ahmad Alhesa, Ala’ Abu Ghalioun, Suzan Khawaldeh, Bayan Shawish, Salem Abu Mahfouz, Mais Al-Shayeb, Shatha Abo Dawoud, Raghad Tlilan, Mohammad Nuseir, Moureq R. Alotaibi, Ola Abu Al Karsaneh, Fida Asali, Marcos Yébenes Mayordomo, Raghda Barham, Rame Khasawneh, Tareq Saleh

“…Methods We utilized a set of patient-derived premalignant and malignant tissue samples to investigate the protein (Ki67 and Lamin B1) and gene (TP53, IL1A, CCL2, and MMP9) expression of several OIS-associated biomarkers using immunohistochemistry (IHC) and qRT-PCR, respectively. Furthermore, we characterized the HPV status of all tissue samples. …”
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NCAPD3-mediated ferroptosis of 2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-glucoside inhibits proliferation in T47D cells by Jianfen Shen, Shuo Zhang, Shuo Zhang, Yan Song, Yan Song, Leiming Yang, Leiming Yang, Qi Huang, Qi Huang, Pengyu Wang, Pengyu Wang, Youzhi Zhang, Youzhi Zhang

“…And the hub genes (transcripts) were selected using the bioinformatics analysis, quantitative polymerase chain reaction (qPCR) and Western blotting (WB). Moreover, cell cycle and ferroptosis were assessed using flow cytometry analysis and WB respectively. …”
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Grotius e Rousseau: duas concepções distintas de soberania by Anderson Francisco dos Santos

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Lnc-EST885 promotes hepatocellular carcinoma metastasis through PI3K / AKT pathway by interaction with TRAF4 by Shaoliang Zhu, Gang Wang, Yuxuan Zhang, Mengjie Zou, Zhi Li, Shenhong Qu, Xiaosu Zou, Wenqian Nong, Weiwei Miao, Qicong Chen, Juanmei Mo, Huibing Chen, Lequn Li, Xiaofeng Dong, Honglin Luo

“…RNA pull-down and ESI-FT-ICR-MS were used to identify proteins that interact with lnc-EST885 and were verified by RIP-qPCR. Furthermore, the association of lnc-EST885 and TRAF4 with HCC prognosis and metastasis was evaluated through bioinformatics analysis and animal models. …”
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Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer by Yvana Cristina Jorge, Mayra Mioto Mataruco, Leandro Pires Araújo, Ana Flávia Teixeira Rossi, Juliana Garcia de Oliveira, Marina Curado Valsechi, Alaor Caetano, Kenji Miyazaki, Célia Sebastiana de Jesus Fazzio, Jorge Alberto Thomé, Paula Rahal, Sonia Maria Oliani, Ana Elizabete Silva

“…We analyzed 40 samples of CG, 20 of GA, and 10 of normal mucosa (C) by the quantitative real-time PCR (qPCR) technique and the immunohistochemistry assay. …”
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Production of ACE inhibitory peptides via ultrasonic-assisted enzymatic hydrolysis of microalgal Chlorella protein: Process improvement, fractionation, identification, and in silic... by Jeeraporn Pekkoh, Apiwit Kamngoen, Antira Wichaphian, May Thu Zin, Supakit Chaipoot, Kamon Yakul, Wasu Pathom-aree, Wageeporn Maneechote, Benjamas Cheirsilp, Kuan Shiong Khoo, Sirasit Srinuanpan

“…Interestingly, the fractionated low molecular weight (LMW) peptides (<3 kDa) demonstrated notable levels of both ABTS radical scavenging (IC50 at 3.34 µg/mL) and ACE inhibition activities (IC50 at 2.95 µg/mL), alongside a significant abundance of essential amino acids, reaching up to 34.98 %. Q-TOF-LC-MS/MS analysis of the LMW peptides verified the appearance of active sequences, identifying up to 49 LMW fragments within the fractionated LMW peptides. …”
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Potential Mechanisms and Effects of Dai Bai Jie Ethanol Extract in Preventing Acute Alcoholic Liver Injury by Niantong Xia, Hongwei Xue, Yihang Li, Jia Liu, Yang Lou, Shuyang Li, Yutian Wang, Juan Lu, Xi Chen

“…Mechanistic investigations were conducted using RT-qPCR assays and immunohistochemical analysis to determine the protective effects of DBJ. …”
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Immunogenic dying cells elicit potent anti-tumor T cell immunity against lung metastasis and tumorigenesis by Min Hu, Xinyu Meng, Tong Wang, Yifan Wang, Xiaodong Chen, Dongliang Xu, Wei He, Hongjia Zhang, Wenzheng Guo, Bo Jing, Siwei Zhang, Jianhua Xu, Beibei Sun, Xueqian Sun, Tingting Liu, Na Ni, Tongtong Zhang, Wenwen Cui, Xiaoyu Wu, Liping Xia, Feng Yao, Fang Zhang, Jing Du, Jiong Deng

“…Cytokines were analyzed by ELISA or Q-PCR analysis. Results Immunization with these live but ICD dying tumor cells induced potent tumor-specific anti-tumor T cell immunity, which not only protected host from challenge by these tumor cells in prevention and therapy in mouse model of lung metastasis, but also prevented tumors development in Gprc5a-ko mouse model of lung tumorigenesis. …”
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Baicalein induces apoptosis by inhibiting the glutamine-mTOR metabolic pathway in lung cancer by Jingyang Li, Di Zhang, Shaohui Wang, Peng Yu, Jiayi Sun, Yi Zhang, Xianli Meng, Juan Li, Li Xiang

“…The overexpression of glutamine transporters was validated using qRT–PCR and western blot analyses. The levels of ASCT2, LAT1, GLS1, p-mTOR, mTOR, and apoptosis-related proteins were evaluated using western blot analysis. …”
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Une inscription méconnue de Cos/Cosa dans la cité des Cadurques by Sabine Armani

“…The beginning of the third line is enigmatic: while the identification of the first letter (a Q) is easy thanks to certain paleographical characteristics, one must be more cautious restoring the word. …”
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Transcriptome analysis unveils the mechanisms of oxidative stress, immunotoxicity and neurotoxicity induced by benzotriazole UV stabilizer-328 in zebrafish embryos by Xinlu Yang, Yijing Gan, Menghuan Zhang, Shaolin Xie, Mingfu Lin, Lixiang Zhong, Mengke Song, Jun Wang, Yumei Huang

“…Moreover, the results of transcriptome analysis and qRT-PCR validation suggested that immune and nerve disorders were caused by UV-328 in zebrafish larvae through regulating the RIG-I-like receptor signaling pathway and neuroactive ligand-receptor interaction, respectively. …”
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Integrating pharmacogenomics and cheminformatics with diverse disease phenotypes for cell type-guided drug discovery by Arda Halu, Sarvesh Chelvanambi, Julius L. Decano, Joan T. Matamalas, Mary Whelan, Takaharu Asano, Namitra Kalicharran, Sasha A. Singh, Joseph Loscalzo, Masanori Aikawa

“…We validate the drugs predicted in our proofs-of-concept using real-time polymerase chain reaction (qPCR) experiments. Results Cell type-specific gene-drug perturbation networks built using QUIZ-C are topologically distinct, reflecting the biological uniqueness of the cell lines in CMap, and are enriched in known drug targets. …”
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Shared mechanisms of enhanced plasmid maintenance and antibiotic tolerance mediated by the VapBC toxin:antitoxin system by Sarah Hollingshead, Gareth McVicker, Maria R. Nielsen, YuGeng Zhang, Giulia Pilla, Rebekah A. Jones, Jonathan C. Thomas, Sarah E. H. Johansen, Rachel M. Exley, Ditlev E. Brodersen, Christoph M. Tang

“…The plasmid stabilizing SNP leads to a Q12L substitution in the DNA-binding domain of VapB, which reduces VapBC binding to its own promoter, impairing vapBC autorepression. …”
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UGCG promotes chemoresistance and breast cancer progression via NF-κB and Wnt/β-catenin pathway activation by Li Long, Lei Wang, Yiran Liang, Fangzhou Ye, Yuhan Jin, Dan Luo, Xiaoyan Li, Yajie Wang, Yaming Li, Dianwen Han, Bing Chen, Wenjing Zhao, Lijuan Wang, Qifeng Yang

“…The role of UGCG in the malignant progression and chemoresistance of TNBC was assessed through various functional assays. Western blotting, qRT-PCR, and immunohistochemistry were employed to investigate the signaling pathways associated with UGCG in TNBC. …”
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Intranasal delivery of hMSC-derived supernatant for treatment of ischemic stroke by inhibiting the pro-inflammatory polarization of neutrophils by Yixiang Jiang, Ning Wang, Jingyi Liu, Haoran Ren, Wenkang Jiang, Yanting Lei, Xidan Fu, Miao Hao, Xiujuan Lang, Yumei Liu, Xijun Liu, Rui Li, Hulun Li

“…The neuroprotective effects of hMSC-L and hMSC-M were assessed with TTC staining, behavioral tests and pathological staining. Flow cytometry and qPCR evaluated the expression of immune cells and cytokines in the CNS and peripheral immune organs. …”
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Tannin alleviated reproductive dysfunction in pregnant ewes infected with Haemonchus contortus by Xin Li, Xin Li, Hai Xiang, Hai Xiang, Rong Liang, Rong Liang, Xinyu Han, Xinyu Han, Rongzhen Zhong, Rongzhen Zhong, Hongyu Liu, Hongyu Liu, Yi Fang, Yi Fang

“…The mRNA levels of hypothalamic–pituitary–ovarian axis-related hormone receptors and placental tissue inflammation genes were detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Finally, fetal weights were measured, and fetal ovarian tissue samples were taken for transcriptomic analysis.ResultsThe results showed that tannins increased the levels of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E2), progesterone (P4), human chorionic gonadotropin (hCG), red blood cell (RBC) counts, packed cell volume (PCV), and mRNA levels of gonadotropic axis receptors in pregnant ewes infected with H. contortus (p &lt; 0.05). …”
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Cross-sectional and longitudinal associations between serum vitamin D and continuous metabolic syndrome score among children and adolescents: roles of levels of inflammation in per... by Yanyan Li, Zhuang Ma, Yan Li, Ting Xiong, Ziyang Zhang, Bingxuan Kong, Wenlong Lu, Xiu Zhao, Rongfei Zheng, Yuhan Tang, Ping Yao, Zhe Su, Yuanjue Wu, Jingfan Xiong

“…GLM revealed that compared to Q1, with the quartiles of serum 25(OH)D concentrations increase, the β value ranged from 0.028 (95% CI: − 0.093, 0.037) to 0.001(95%CI: − 0.067, 0.069), and then to -0.074 (95%CI: -0.146, -0.003, P for trend = 0.0659). …”
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Expression of USP18 and IL2RA Is Increased in Individuals Receiving Latent Tuberculosis Treatment with Isoniazid by Eleane de Oyarzabal, Lourdes García-García, Claudia Rangel-Escareño, Leticia Ferreyra-Reyes, Lorena Orozco, María Teresa Herrera, Claudia Carranza, Eduardo Sada, Esmeralda Juárez, Alfredo Ponce-de-León, José Sifuentes-Osornio, Robert J. Wilkinson, Martha Torres

“…Expression of chemokine ligand 4 (CCL4), chemokine ligand 10 (CXCL10), chemokine ligand 11 (CXCL11), interferon alpha (IFNA), radical S-adenosyl methionine domain-containing 2 (RSAD2), ubiquitin-specific peptidase 18 (USP18), interferon-induced protein 44 (IFI44), interferon-induced protein 44 like (IFI44L), interferon-induced protein tetratricopeptide repeats 1(IFIT1), and interleukin 2 receptor subunit alpha (IL2RA) mRNA levels were assessed by qPCR before, during, and after INH treatment. Results. …”
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Ginsenoside Rd protects against acute liver injury by regulating the autophagy NLRP3 inflammasome pathway by Xiaomei Zhong, Yibin Sun, Yanxiang Lin, Shan Deng, Huan Wang, Xian Zhou, Jinjian Lu, Yanfang Zheng, Ruoyin Luo, Mingqing Huang, Jianyuan Song

“…Immunofluorescence staining, qPCR and Western blot were employed to analyze the expressions of genes and proteins associated with inflammation and autophagy. …”
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