Nucleotide receptor P2X7/STAT6 pathway regulates macrophage M2 polarization and its application in CAR-T immunotherapy by Qin Si, Lu Yang, Jie Liu, Hui Liu, Ruifang Bu, Na Cui

“…After silencing P2X7 receptor and/or overexpressing STAT6 within macrophages, the M1 and M2 markers were evaluated via flow cytometry, ELISA, and qRT-PCR. Additionally, the phosphorylation level of STAT6 was monitored by western blot. …”
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Characterization of rectus femoris lesions in knee osteoarthritis at different stages and the effect of ultrasound-guided acupotomy by Wen-Ying Yu, Wen-Ying Yu, Jing Liu, Jing Liu, Ze-Hao Lin, Ze-Hao Lin, Hong Liu, Hong Liu, Liang-Zhi Zhang, Liang-Zhi Zhang, Xiang-Long Feng, Xiang-Long Feng, Zhong-Biao Xiu, Zhong-Biao Xiu, Zhong-Biao Xiu

“…Additionally, ultrasound-guided acupotomy operations were performed on the rabbit model of late-stage KOA, and alterations in the key molecular markers of rectus femoris fibrosis were determined using Western Blot and qPCR methods.ResultsAs the disease progressed, the elastic modulus of the rectus femoris muscle in KOA rabbits gradually increased, the cross-sectional area gradually decreased, and the degree of fibrosis increased. …”
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Jianpi Yiqi Busui prescription alleviates myasthenia gravis by regulating Th17 through the TAK1/P38 MAPK/eIF-4E signaling pathway by Zhuming Chen, Jing Lu, Tianying Chang, Dongmei Zhang, Yibin Zhang, Miao Liu, Tong Wu, Peng Xv, Jian Wang

“…Flow cytometry was utilized to count Th17, Th1, Th2, and Treg cells in lymphocytes. ELISA and RT-qPCR were used to measure acetylcholine receptor antibody (AChR-Ab) and Th17-related cytokines, including IL-17, IL-21, IL-23, TNF-α, TGF-β, IL-1β, and IL-6. …”
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GmERF57 negatively regulates root development and phosphate absorption in soybean by Hongqing Zhu, Dandan Hu, Yifei Yang, Xuhao Zhai, Shanshan Zhang, Mengshi He, Huifang Zuo, Lina Zhang, Mengjun Xu, Shanshan Chu, Haiyan Lü, Hengyou Zhang, Yu Zhang, Dan Zhang

“…Previously, we identified soybean Ethylene Response Factor 57 (GmERF57) as a candidate gene responsible for q20, a major QTL associated with soybean low Pi (LP) tolerance-related traits identified through QTL mapping and genome-wide association analysis (GWAS). …”
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Genome-Wide Characterization and Expression Analysis of the HD-ZIP Gene Family in Response to Salt Stress in Pepper by Zhongrong Zhang, Ranran Zhu, Xuehua Ji, Hui Ji Li, Hui Lv, Hai Ying Zhang

“…CaHDZ03 possesses two closely linked ABA action elements, and its expression level increased significantly at 4 h under salt stress. qRT-P-CR and transcription analysis showed that the expression of CaHDZ03 and CaHDZ10 was upregulated under short-term salt stress, but CaHDZ10 was downregulated with long-term salt stress, which provided a theoretical basis for research the function of Ca-HDZIP in response to abiotic stress.…”
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Identification of novel serum lipid metabolism potential markers and metabolic pathways for oral cancer: a population-based study by Na Wang, Yujia Chen, Jianli Lin, Yulan Lin, Haoyuan Song, Weihai Huang, Liling Shen, Fa Chen, Fengqiong Liu, Jing Wang, Yu Qiu, Bin Shi, Ling Li, Lisong Lin, Lizhen Pan, Baochang He

“…Methods A total of 41 OC patients and 41 controls were recruited from a hospital in Southeast China to examine the serum lipidomics by Ultra-high Performance Liquid Chromatography Q Exactive Mass Spectrometry (UHPLC-QE-MS). Results The total serum lipid profile showed that triglycerides accounted for the highest proportion of total metabolites, reaching 35.90% of the total. …”
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FKBP5 as a key regulator of metabolic processes in birds: Insights from chicken pectoral muscle by Pengfei Du, Xiangli Zhang, Yao Zhu, Ziyang Wang, Xuemeng Si, Huaiyong Zhang, Yanqun Huang, Wen Chen

“…Real-time quantitative PCR (RT-qPCR) was utilized to analyze the spatiotemporal expression patterns of FKBP5 in chickens. …”
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Identification and functional analysis of Wall-Associated Kinase genes in Nicotiana tabacum by Ling Li, Linggai Cao, Jintao Li, Zhiqiang Zhang, Zhiqiang Zhang, Jie Liu, Zhongying Ren, Jie Zhang, Rengang Wang, Yangfan Miao, Shizhou Yu, Wei Li, Wei Li

“…The expression levels of NtWAK genes were assessed by qRT-PCR. The subcellular localization of WAK proteins was observed in tobacco cells and Arabidopsis protoplasts. …”
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Clinical Outcomes in Diabetic Females Presenting with STEMI: A Cohort Study by Anjana Ajith Beena, Priya Shanmukhan, Veena Felix, Alummoottil George Koshy, Kunjukrishnapilla Sivaprasad

“…Among those with in-hospital mortality, a higher proportion had Anterior Wall Myocardial Infarction (AWMI) (p=0.043), were in Killip class>II (p-value <0.0001), and had qRBBB (Right Bundle Branch Block) (p-value <0.0001). …”
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Application of copy number variation sequencing combined with whole exome sequencing in prenatal left–right asymmetry disorders by Yu Qin, Muon Senglong, Koksear Touch, Juan Xiao, Ruijie Fang, Qingling kang, Lei Fan, Shufang Li, Jing Liu, Jianli Wu, Yuanyuan Wu, Xinwei Shi, Haiyi Liu, Xun Gong, Xingguang Lin, Ling Feng, Suhua Chen, Wei Li

“…CNV-seq revealed a 16p11.2 microdeletion syndrome in a situs inversus fetus presenting pathogenic and a 2q36.3 microduplication syndrome in a fetus with Heterotaxy presenting a variant of uncertain significance (VUS). …”
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Effet de l'illumination proche infra-rouge intracérébrale chez des primates MPTP by Fannie Darlot, Cécile Moro, Napoléon Torres‑Martinez, Claude Chabrol, Florian Reinhart, Diane Agay, Thomas Costecalde, John Mitrofanis, Alim‑Louis Benabid

“…Des études antérieures ont montré que des molécules, dont CoQ10 et la mélatonine, ont un effet neuroprotecteur sur les cellules DA de la SNc contre la dégénérescence induite dans des modèles animaux de la MP qui induisent un dysfonctionnement mitochondrial. …”
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CircUCK2(2,3) promotes cancer progression and enhances synergistic cytotoxicity of lenvatinib with EGFR inhibitors via activating CNIH4–TGFα–EGFR signaling by Xindong Wei, Anfeng Si, Shuai Zhao, Yi Fu, Jilei Li, Kedeerya Aishanjiang, Yujie Ma, Chang Yu, Bo Yu, Chunhong Cui, Hui Wang, Xianming Kong, Shibo Li, Xiaoni Kong, Ying Tong, Hailong Wu

“…CircUCK2(2,3) levels were measured in two independent HCC cohorts using quantitative real-time PCR (qRT–PCR). We explored the functions of circUCK2(2,3) using gain- and loss-of-function assays. …”
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Efficacy and safety of PD-1/PD-L1 inhibitors in advanced or recurrent endometrial cancer: a meta-analysis with trial sequential analysis of randomized controlled trials by Ji Ren, Jinghe Wang, Yanan Wang, Dongying Yang, Jianming Sheng, Shili Zhu, Yunli Liu, Xiaoqi Li, Wei Liu, Binbin Zhang

“…To evaluate heterogeneity, we employed Cochran’s Q test, I2 statistics, and 95% prediction intervals (PIs). …”
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Hypothyroidism impairs the circadian rhythmicity of clock genes and proteins involved in gut nutrient absorption in female mice by Ayla Secio-Silva, Paulo Henrique Evangelista-Silva, Felipe Emrich, Letícia Selvatici-Tolentino, Maíza Ferreira, Ana Bárbara de Paula Silva, Bruno Henrique Gomes, Tatienne Neder Figueira-Costa, André Gustavo Oliveira, Rodrigo Antonio Peliciari-Garcia, Francemilson Goulart-Silva, Paula Bargi-Souza

“…The proximal small intestine segment was collected and immediately frozen for gene expression analysis of circadian core clock components (Bmal1, Per2, Cry1, and Nr1d1) and nutrient transporters by RT-qPCR. The daily protein content of nutrient transporters involved in the absorption of the products of hydrolysis of lipids, proteins, and carbohydrates was evaluated over 24 h in isolated small intestinal epithelium by Western blotting. …”
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CFTR Expression Analysis for Subtyping of Human Pancreatic Cancer Organoids by Alexander Hennig, Laura Wolf, Beatrix Jahnke, Heike Polster, Therese Seidlitz, Kristin Werner, Daniela E. Aust, Jochen Hampe, Marius Distler, Jürgen Weitz, Daniel E. Stange, Thilo Welsch

“…Quantitative real-time PCR (qPCR) was performed for CFTR and HNF1A. A chemotherapeutic drug response analysis was done using gemcitabine, 5-FU, oxaliplatin, and irinotecan. …”
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Gut microbiota influence on lung cancer risk through blood metabolite mediation: from a comprehensive Mendelian randomization analysis and genetic analysis by Yizhao Du, Qin Wang, Zongmei Zheng, Hailun Zhou, Yang Han, Ao Qi, Lijing Jiao, Lijing Jiao, Yabin Gong

“…MR-Egger intercept test, Radial MR, MR-PRESSO, Cochran Q test and Leave-one-out (LOO) analysis were used for sensitivity analyses. …”
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Transcriptomic signatures of cold acclimated adipocytes reveal CXCL12 as a Brown autocrine and paracrine chemokine by Marina Agueda-Oyarzabal, Marie S. Isidor, Kaja Plucińska, Lars R. Ingerslev, Oksana Dmytriyeva, Patricia S.S. Petersen, Sara Laftih, Axel B. Pontoppidan, Jo B. Henningsen, Kaja Rupar, Erin L. Brown, Thue W. Schwartz, Romain Barrès, Zachary Gerhart-Hines, Camilla C. Schéele, Brice Emanuelli

“…Cxcl12 mRNA expression analysis by RT-qPCR and fluorescence in situ hybridization specified Cxcl12 distribution in various cell types, and indicated its enrichment in cold-acclimated brown adipocytes. …”
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The Expression Pattern and Functional Analysis of Extracellular Vesicle Long Non-Coding RNAs from Uterine Fluid During Implantation in Pig by Yijun Shang, Qiuping Zhang, Yue Ding, Yongzhong Wang, Shengchen Gu, Xupeng Zang, Zheng Xu, Sixiu Huang, Zicong Li, Zhenfang Wu, Ting Gu, Linjun Hong, Gengyuan Cai

“…A total of 30,203 lncRNAs were identified and 7879 differentially expressed lncRNAs were screened, and qRT-PCR was used to verify the sequencing data. …”
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Pharmacological approaches in drug-resistant pediatric epilepsies caused by pathogenic variants in potassium channel genes by Ilaria Filareto, Ilaria Mosca, Elena Freri, Francesca Ragona, Laura Canafoglia, Roberta Solazzi, Barbara Castellotti, Giuliana Messina, Cinzia Gellera, Maria Virginia Soldovieri, Paolo Ambrosino, Maurizio Taglialatela, Jacopo C. DiFrancesco, Tiziana Granata

“…The application in vivo of fluoxetine to counteract GoF effects induced by Kv3.1 or KNa1.1 variants determined a significant reduction of both seizure frequency and behavior disturbances in patient #4 (Kv3.1 V425M), and in both subjects carrying KNa1.1 variants (#5, S937G and #6, R262Q). However, for the latter case, this drug was halted due to severe behavioral side effects. …”
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A rare haplotype of the GJD3 gene segregating in familial Meniere’s disease interferes with connexin assembly by Alba Escalera-Balsera, Paula Robles-Bolivar, Alberto M. Parra-Perez, Silvia Murillo-Cuesta, Han Chow Chua, Lourdes Rodríguez-de la Rosa, Julio Contreras, Ewa Domarecka, Juan Carlos Amor-Dorado, Andrés Soto-Varela, Isabel Varela-Nieto, Agnieszka J. Szczepek, Alvaro Gallego-Martinez, Jose A. Lopez-Escamez

“…The connexin monomer and the homomeric connexon structural models were predicted using AlphaFold2 and HDOCK. RT-qPCR and immunofluorescence were done in mice cochleae to identify expression of the mouse ortholog candidate gene Gjd3. …”
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