Narratives of swiss football players about talent - and positive youth development: The role of social agents and agency. by Philipp Koch, Bryan Charbonnet, Noora Ronkainen

“…., Warner, S., & King, M. Q. (2022). Toward an integrated understanding of the youth sport system. …”
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Arthralgia and Extraintestinal Manifestations in Crohn’s Disease Elevate the Risk of IBD-Related Arthritis over Sacroiliitis by Ivan Giovannini, Nicola Cabas, Marco Marino, Annarita Tullio, Ilaria Tinazzi, Angela Variola, Carmelo Cicciò, Fabro Cinzia, Berretti Debora, Chiara Zuiani, Rossano Girometti, Luca Quartuccio, Alen Zabotti, Lorenzo Cereser

“…Results Ninety-five patients, mean age 43.9 years (standard deviation [SD] ± 16.6), 52.6% female were enrolled in the study with a median follow-up of 83 months (Q25:75 25:143). Six out 95 (6.3%) developed IBD-related arthritis with a mean time of 11 months (SD ± 16.8). …”
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Targeting deubiquitinase USP7-mediated stabilization of XPO1 contributes to the anti-myeloma effects of selinexor by Junying Wang, Mengping Chen, Jinxing Jiang, Yike Wan, Xin Li, Minyue Zhang, Fei Xiao, Lu Zhong, Hua Zhong, Zhaoyu Qin, Jian Hou

“…Methods We assessed XPO1 protein and mRNA levels using western blotting and RT-qPCR. Cycloheximide (CHX) chase assays and degradation blockade assays were used to determine the pathway of XPO1 degradation induced by SEL. …”
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Niacin alters ruminal microbial composition and metabolites in sheep fed a high-concentrate diet by Zhiqiang Cheng, Jiancheng Liu, Yingying Yu, Wentao Liu, Xiaobin Li, Fengming Li, Changjiang Zang, Kailun Yang

“…The concentrations of NH3-N (p < 0.01), propionate (p < 0.01), and butyrate (p < 0.05) in the NA group were significantly higher than those in the CON group. (3) Compared to the CON group, the ruminal pyruvate content in the NA group was significantly increased at 0 h before feeding (p < 0.05), and lactic acid (p < 0.05) was significantly decreased at 1 and 3 h after feeding, lactate dehydrogenase activities was significantly decreased (p < 0.01) at 3 and 5 h after feeding. (4) The number of specific operational taxonomic units (OTUs) in the CON and NA groups were 26 and 37, respectively, for a total of 1,178 OTUs; principal coordinate analysis (R2 = 0.172, p-value = 0.007) and non-metric multidimensional scaling (stress = 0.1646) results showed that the two groups of samples were significantly separated. (5) The species distribution bar graph shows that at the phylum level, the relative abundances of Bacteroidetes, Firmicutes, and Proteobacteria were 43.70, 36.25, and 12.77%, respectively. (6) Orthogonal projection to latent structure-discriminant analysis results showed that the two groups of samples were clearly separated in the positive and negative ionization modes, with R2Y and Q2Y values of 0.705, 0.857, 0.695, and 0.28, respectively. …”
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Environmental surveillance of SARS-CoV-2 for outbreak detection in hospital: A single centre prospective study by Hania Siddiqui, Alexandra M.A. Hicks, Aaron Hinz, Prachi Ray, Jennie Johnstone, Derek R. MacFadden, Jason A. Moggridge, Michael Fralick

“…Swabs were assayed for SARS-CoV-2 by quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR). Results from the floor swabs, including percentage positivity for SARS-CoV-2 and number of viral RNA copies, were sent to the hospital's infection control team twice-weekly. …”
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Validating a clinically based MS-MLPA threshold through comparison with Sanger sequencing in glioblastoma patients by Halka Lhotska, Karolina Janeckova, Hana Cechova, Jaromir Macoun, Tatiana Aghova, Libuse Lizcova, Karla Svobodova, Lucie Hodanova, Dora Konecna, Jiri Soukup, Filip Kramar, David Netuka, Zuzana Zemanova

“…Reduced expression of the MGMT gene (10q26.3), influenced primarily by the methylation of two differentially methylated regions (DMR1 and DMR2), is associated with a good response to temozolomide treatment. …”
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Serum hsa-miR-22-3p, hsa-miR-885-5p, Lipase-to-Amylase Ratio, C-Reactive Protein, CA19-9, and Neutrophil-to-Lymphocyte Ratio as Prognostic Factors in Advanced Pancreatic Ductal Ade... by Jakub Wnuk, Dorota Hudy, Joanna Katarzyna Strzelczyk, Łukasz Michalecki, Kamil Dybek, Iwona Gisterek-Grocholska

“…The relative expression levels of miR-22-3p and miR-885-5p were measured using RT-qPCR. Serum hsa-miR-22-3p was detected in 22 cases (44%), while hsa-miR-885-5p was detected in 33 cases (66%). …”
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Influence of preoperative variables on the 3-month functional outcomes of the Vivity extended depth-of-focus intraocular lens: a prospective case series by Giacomo Savini, Alice Galzignato, Catarina P. Coutinho, Jinhai Huang, Yue Wu, Piero Barboni, João Mendanha Dias, Filomena J. Ribeiro, Domenico Schiano-Lomoriello

“…Regression analysis did not disclose any significant correlation between the preoperatively measured variables and the postoperative outcomes, with a few exceptions: preoperative higher order corneal aberrations were correlated to halometry area (r2 = 0.2592, P = 0.0006) and the Q value to contrast sensitivity (r2 = 0.1717, P = 0.00574) under photopic conditions with glare at a spatial frequency of 18 cpd and without glare for all spatial frequencies (P < 0.01); it was also correlated to contrast sensitivity under mesopic conditions without glare at a spatial frequency of 12 cpd (r2 = 0.2311, P = 0.0011). …”
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Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats by Esra Aslan, Gokhan Sadi, Hilal Guzel, Cigdem Karaca, Omer Adil Korkmaz, Mine Kanat Pektas, Mehmet Celegen, Tugce Aladag, Seyma Oncu, Mehmet Bilgehan Pektas

“…Gene expressions were evaluated with semi-quantitative real-time polymerase chain reaction (qRT-PCR). The indirect terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) method was used to assess changes in apoptotic cells, and hematoxylin/eosin staining to determine adipocyte number and diameter. …”
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Glycated Serum Protein Genetics and Pleiotropy with Cardiometabolic Risk Factors by Matthew P. Johnson, Ryan Keyho, Nicholas B. Blackburn, Sandra Laston, Satish Kumar, Juan Peralta, Suman S. Thapa, Bradford Towne, Janardan Subedi, John Blangero, Sarah Williams-Blangero

“…We localized a significant (LOD score=3.18) and novel GSP QTL on chromosome 11q, which has been previously linked to type 2 diabetes. …”
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D-mannose alleviates chronic periodontitis in rats by regulating the functions of neutrophils by Xue Li, Xueting Chen, Qingyu Zhu, Pengye Zhang, Shunxue Nan, Lei Lv, Shengcai Qi

“…After D-mannose treatment, the expression levels of IL-17 (p<0.01) and TET2 (p<0.01) were suppressed by IF, and the expression levels of IL-1β (p<0.05), IL-17 (p<0.05) and TET2 (p<0.01) were downregulated by WB. The results of qPCR showed that D-mannose reduced the expression levels of IL-1β (p<0.05), IL-6 (p<0.01), IL-17 (p<0.01), TNF-α (p<0.01), G-CSF (p<0.01), GM-CSF (p<0.01), TET2 (p<0.01), HK1 (p<0.01), HK2 (p<0.01), and PFKFB3 (p<0.01). …”
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Shorter telomere length in COPD cases secondary to biomass-burning smoke exposure by Angélica Domínguez-de-Barros, Gloria Pérez-Rubio, Ingrid Fricke-Galindo, Alejandra Ramírez-Venegas, Malena Gajate-Arenas, Rafael Hernández-Zenteno, Salvador García-Carmona, Robinson Robles-Hernández, María E. Ramírez-Díaz, Filiberto Cruz-Vicente, María L. Martínez-Gómez, Jacob Lorenzo-Morales, Ramcés Falfán-Valencia, Elizabeth Córdoba-Lanús

“…Lung function parameters were measured by spirometry, and relative telomere length (rTL) from peripheral blood DNA was determined using multiplex qPCR. Results Results showed rTL to inversely correlate with age (R2=-0.207, p = 0.006) and with the hours-a-day of BS exposure (R2=-0.297, p < 0.001). …”
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Computational and in vitro evaluation of sumac-derived ©Rutan compounds towards Sars-CoV-2 Mpro inhibition by Muzaffar Kayumov, Parthiban Marimuthu, Parthiban Marimuthu, Jamoliddin Razzokov, Jamoliddin Razzokov, Jamoliddin Razzokov, Nurkhodja Mukhamedov, Nurkhodja Mukhamedov, Akmal Asrorov, Nodir S. Berdiev, Jamolitdin F. Ziyavitdinov, Ansor Yashinov, Ansor Yashinov, Yuliya Oshchepkova, Shavkat Salikhov, Sharafitdin Mirzaakhmedov

“…Furthermore, the per-residue decomposition analysis showed C145, E166, and Q189 residues as the hotspot residues for components contributing to maximum BFE energies. …”
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Profiling of Somatic Mutations in Phaeochromocytoma and Paraganglioma by Targeted Next Generation Sequencing Analysis by Andrea Luchetti, Diana Walsh, Fay Rodger, Graeme Clark, Tom Martin, Richard Irving, Mario Sanna, Masahiro Yao, Mercedes Robledo, Hartmut P. H. Neumann, Emma R. Woodward, Farida Latif, Stephen Abbs, Howard Martin, Eamonn R. Maher

“…Mutations were identified for HRAS (c.37G>C; p.G13R and c.182A>G; p.Q61R) in 7.1% (6/85); for BRAF (c.1799T>A; p.V600E) in 1.2% (1/85) of tumours; and for TP53 (c.1010G>A; p.R337H) in 2.35% (2/85) of cases. …”
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Co-infection of SARS‐CoV‐2 and influenza A/B among patients with COVID-19: a systematic review and meta-analysis by Monireh Golpour, Hossein Jalali, Reza Alizadeh-Navaei, Masoumeh Rezaei Talarposhti, Tahoora Mousavi, Ali Asghar Nadi Ghara

“…Heterogeneity of study results was evaluated using the I-square and Q index, and publication bias was examined using the Begg’s and Egger’s tests, as well as funnel plot. …”
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Ethanol extract of Polygonatum cyrtonema Hua mitigates non-alcoholic steatohepatitis in mice by Dongliang Chen, Yue Shen, Yue Shen, Fang Huang, Bo Huang, Shangfu Xu, Shangfu Xu, Lisheng Li, Lisheng Li, Jie Liu, Zheng Li, Xia Li

“…One hand, liver injury was assessed by serum enzymes, biochemistry, and histopathology; On the other hand, RNA-seq, qPCR, and Western blot were employed to investigate the related molecular mechanisms.Results211 metabolites were identified through UHPLC-MS/MS analysis. …”
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3D bioprinting of engineered exosomes secreted from M2-polarized macrophages through immunomodulatory biomaterial promotes in vivo wound healing and angiogenesis by Sayan Deb Dutta, Jeong Man An, Jin Hexiu, Aayushi Randhawa, Keya Ganguly, Tejal V. Patil, Thavasyappan Thambi, Jangho Kim, Yong-kyu Lee, Ki-Taek Lim

“…Transcriptomic (RNA-Seq) and real-time PCR (qRT-PCR) study revealed that co-culture of fibroblast/keratinocyte/stem cells/endothelial cells in a 3D bioprinted COL@d-ECM/M2-Exo hydrogel upregulated the skin-associated signature biomarkers through various regulatory pathways during epidermis remodeling and downregulated the mitogen-activated protein kinase (MAPK) signaling pathway after 7 days. …”
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Carboxylesterase 4A Inhibits the Malignant Biological Behavior of Nasopharyngeal Carcinoma via the PI3K/AKT Pathway by Qiaoli Chen MS, Quanxiang Hao MBBS, Yanping Yang MBBS, Limei Li PhD, Danping Li MS, Ran Zhao MS, Wanqi Wei MBBS, Lixian Deng MBBS, Jiaming Su MBBS, Ziyuan Liang MBBS, Shiyue Tang MS, Yaomin Lu MBBS, Yushan Liang PhD, Zhe Zhang PhD, Xiaoying Zhou PhD, Xue Xiao PhD, Ping Li PhD, Yi Huang PhD, Weilin Zhao PhD

“…Method A standardized mean difference (SMD) analysis was used to analyze the dysregulation of CES4A based on the gene expression omnibus (GEO) database. qRT-PCR and immunohistochemical staining (IHC) were used to identify the mRNA and protein levels of CES4A in NPC cell lines and tissues, respectively. …”
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SUMO-Specific Peptidase 5 Promotes Oesophageal Squamous Cell Carcinoma Growth through the NF-κB-SLC1A3 Axis by Chaoxiang Du, Yunfan Hu, Xinyu Yang, Zhe Zhang, Jianmin Gu, Tao Zhang, Renfeng Wang, Shaoyuan Zhang, Lijie Tan, Guiping Yu

“…The correlation of SENP5 with signalling pathway components was validated via real-time quantitative PCR (qPCR), western blotting (WB), and immunoprecipitation. …”
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Establishment of <i>Echinococcus granulosus EgM123</i> Recombinant Gene Rabies Virus SRV₉ and Identification of Its Biological Characteristics by Yueqi Yang, Mengdan Hou, Guicheng Su, Xiaoyan Ma, Xiaohui Su, Kunlei Li, Songhan Liu, Luheng Xiao, Jingjing Yao, Jiahao Zhai, Xiaoying Wei, Yang Zhou, Qianqian Lai, Yuwei Dong, Jieyu Liu, Shaohua Zhai

“…The rescued virus-infected BSR cells were characterized by TEM, and the results indicated that bullet-like viral particles with an average size of 148.47 nm and a cyst structure were present in the cytoplasm of BSR cells; the expression levels of continuously cultivated 9th-, 10th-, 11th-, 12th-, and 13th-generation viruses were quantified by qRT-PCR, and the results showed that mRNA expression of the virus was upregulated. …”
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