Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese by Jin Yu, Ichraf Mabrouk, Qiuyuan Liu, Yuxuan Zhou, Yupu Song, Jingyun Ma, Fengshuo Liu, Xiangman Hu, Zhiyi Yang, Yuxia Zeng, Jingtao Hu, Yongfeng Sun

“…Additionally, the expression trends of 4 identified DEGs were validated by RT-qPCR. In conclusion, this study elucidates the potential regulatory role of melatonin in gonadal development in geese, identifying candidate genes involved in early gonadal differentiation and maturation. …”
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CircPRMT5, a Potential Salivary Biomarker, Facilitates the Progression of Head and Neck Squamous Cell Carcinoma via the IGF2BP3-SERPINE1 Pathway by Jiang S, Ou L, Wang Y, Su K, Chen Z, He L, Xu X, Cheng B, Xia J, Fan Z

“…Finally, salivary exosomes were isolated, and the expression levels of circPRMT5 were assessed using qRT-PCR.Results: The upregulation of circPRMT5 in HNSCC tissues was identified to be correlated with cervical lymph node metastasis and advanced clinical T stage. …”
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Comprehensive analysis of metabolomics and transcriptomics reveals varied tepal pigmentation across Gloriosa varieties by Yue Sun, Pinli Hu, Yanan Jiang, Zhenzhen Wang, Jiaxing Chang, Yiwei Zhou, Haojing Shao

“…Weighted gene co-expression network analysis (WGCNA) revealed significant co-expression patterns between 217 transcription regulatory factors (TFs) and 8 carotenoid biosynthesis genes, and between 194 TFs and 41 anthocyanin biosynthesis genes. qRT-PCR verified the expression patterns of four carotenoid biosynthesis-related genes, eight anthocyanin biosynthesis-related genes, and three transcription regulatory factors. …”
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Inducing salt stress tolerance in bitter gourd (Momordica chanrantia) through seed treatment with chitosan by Ahsan Ali, Fiaz Hussain Ferdosi, Mubeen Sarwar, Sumreen Anjum, Zain Mushtaq, Mehwish Liaquat, Muhammad Taqqi Abbas, Moazzam Anees, Muhammad Rizwan Tariq, M. Irfan Ashraf, Abdulrahman Alasmari, Md Sabir Ahmed Mondol, Gholamreza Abdi

“…MethodsThe present study was designed to evaluate the various morphological attributes (mean germination time, germination index, final emergence percentage, measurements of root length, measurement of shoot length, measurement of plant dry biomass, and measurement of plant fresh biomass), physiological attributes (leaf chlorophyll content and electrolyte leakage), biochemical attributes (proline contents, antioxidant enzymes, superoxide dismutase, catalase Q9 , and peroxidase), leaf water relations (leaf osmotic potential, leaf water potential, leaf turgor potential, and leaf relative water content), and ion concentrations (Na+, K+, Ca +, and Cl-) that can be used for the evaluation of salt stress tolerance potential in bitter gourd. …”
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Hypermethylation in Calca Promoter Inhibited ASC Osteogenic Differentiation in Rats with Type 2 Diabetic Mellitus by Lei Wang, Feng Ding, Shaojie Shi, Xingxing Wang, Sijia Zhang, Yingliang Song

“…CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca. …”
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Reduced Circulating Levels of miR-491-5p and miR-485-3p Are Associated with the Occurrence of Vertebral Fractures in Postmenopausal Women with Osteoporosis by Jixi Xu, Mingbo Li, Wei Pei, Jinyong Ding, Yueran Pan, Huifeng Peng, Shiman Lin, Yanbo Huang

“…The expression levels of miR-491-5p and miR-485-3p were determined by qRT-PCR. Pearson correlation analysis was carried out to assess the relationship between miR-491-5p, miR-485-3p, and bone metabolic markers. …”
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HDC1 Promotes Primary Root Elongation by Regulating Auxin and K⁺ Homeostasis in Response to Low-K⁺ Stress by Xiaofang Kuang, Hao Chen, Jing Xiang, Juan Zeng, Qing Liu, Yi Su, Chao Huang, Ruozhong Wang, Wanhuang Lin, Zhigang Huang

“…Moreover, the organ-specific RT-qPCR analyses unraveled that HDC1 negatively regulates the expression of CBL-CIPK-K⁺ channel-related genes such as <i>CBL1, CBL2</i>, <i>CBL3</i>, <i>AKT1,</i> and <i>TPK1</i>, thereby establishing a molecular link between histone deacetylation, auxin signaling, and CBLs-CIPKs pathway in response to K⁺ deficiency.…”
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Identifying disulfidptosis-related biomarkers in epilepsy based on integrated bioinformatics and experimental analyses by Sijun Li, Lanfeng Sun, Hongmi Huang, Xing Wei, Yuling Lu, Kai Qian, Yuan Wu

“…G protein guanine nucleotide-binding protein alpha subunit q (GNAQ) was linked to sodium valproate resistance. …”
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Upregulated astrocyte HDAC7 induces Alzheimer-like tau pathologies via deacetylating transcription factor-EB and inhibiting lysosome biogenesis by Jinwang Ye, Suyue Zhong, Huali Wan, Xing Guo, Xuanbao Yao, Qiong Liu, Liming Chen, Jian-Zhi Wang, Shifeng Xiao

“…Immunoprecipitation, immunofluorescence, western blotting, RT-qPCR, mass spectrometric, and luciferase reporter assay were used to identify HDAC7 substrates, modification site and related signaling pathways in astrocyte-tau clearance. …”
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Hypoxia-Elicited Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Alleviate Myocardial Infarction by Promoting Angiogenesis through the miR-214/Sufu Pathway by Lianbo Shao, Yihuan Chen, Jingjing Li, Jingfan Chao, Ziying Yang, Yinglong Ding, Han Shen, Yueqiu Chen, Zhenya Shen

“…The potential mechanism by which MSChyp-sEVs enhance angiogenesis was explored in vitro by RT–qPCR and western blotting. Results. Tube formation and scratch assays demonstrated that the proangiogenic capability of huMSC-derived sEVs was enhanced by hypoxia pretreatment. …”
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Integrated Impacts of Urban Spatial Form on Thermal Environment and Zonal Regulation under the Perspective of Spatial Heterogeneity by Yan Jinlong, Yin Chaohui, An Zihao, Zhang Simin, Wen Qian, Chen Weiqiang

“…Second, the three-dimensional (3D) building indicator had a greater overall impact on the thermal environment than the two-dimensional (2D) urban land-use type indicator. Building density (q = 0.479) was the dominant factor affecting the thermal environment. …”
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Prevalence of Salmonella and Shigella species and their multidrug resistance patterns among pediatric populations in Ethiopia: a systematic review and meta-analysis by Mihret Tilahun, Melaku Ashagrie Belete, Alemu Gedefie, Habtu Debash, Ermiyas Alemayehu, Daniel Gebretsadik Weldehana, Hussein Ebrahim, Ousman Mohammed, Bruktawit Eshetu, Saba Gebremichael Tekele, Zewudu Mulatie, Agumas Shibabaw

“…Heterogeneity was assessed using the Cochrane Q test and I2 statistics with corresponding p-values. …”
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Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress by Yong-Juan Mo, Yi-Li Gao, Shi-Jjian Xu, Bin Wang, Jingping Wu, Peng Yu, Ming Li, Xiangtao Kang, Zhi-Qiang Han

“…The sturgeons were then allocated into four experimental groups for salt stress exposure; one control group was maintained in freshwater, while the others were subjected to salinities of 8 ‰, 16 ‰, and 24 ‰, respectively. Quantitative PCR (qPCR) and western blot analysis were employed to assess Atrogin-1 expression levels under high salt stress. …”
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Genome-Wide and Gene-Specific Epigenomic Platforms for Hepatocellular Carcinoma Biomarker Development Trials by Christina Michailidi, Ethan Soudry, Mariana Brait, Leonel Maldonado, Andrew Jaffe, Carmen Ili-Gangas, Priscilla Brebi-Mieville, Jimena Perez, Myoung Sook Kim, Xiaoli Zhong, Quiang Yang, Blanca Valle, Stephen J. Meltzer, Michael Torbenson, Manel Esteller, David Sidransky, Rafael Guerrero-Preston

“…Promoter hypermethylation of RASSF1A, SSBP2, and B4GALT1 quantified by qMSP had a sensitivity ranging from 38% to 52%, a specificity of 100%, and an AUC from 0.58 to 0.75. …”
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MicroRNA Expression in Cutaneous Lupus: A New Window to Understand Its Pathogenesis by Silvia Méndez-Flores, Janette Furuzawa-Carballeda, Gabriela Hernández-Molina, Gustavo Ramírez-Martinez, Nora E. Regino-Zamarripa, Blanca Ortiz-Quintero, Luis Jiménez-Alvarez, Alfredo Cruz-Lagunas, Joaquín Zúñiga

“…We included 22 patients with subacute (SCLE) and 20 with discoid (DLE) lesions, and 19 healthy donors (HD). qRT-PCR for miRNA analysis, flow cytometry in peripheral blood, and skin immunohistochemistry were performed to determine the distribution of CD4 T cells and regulatory cells and their correlation with circulating miRNAs. …”
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Laboratory Measurement of CH317OH Transitions in the Frequency Range from 216 to 264 GHz for Astronomical Application by Akemi Tamanai, Takahiro Oyama, Yoshimasa Watanabe, Takeshi Sakai, Riouhei Nakatani, Shaoshan Zeng, Isabelle Kleiner, Nami Sakai

“…We have specifically paid attention to the Q -branch transitions, which are the strongest line series in this frequency region. …”
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Cyclopiazonic acid suppresses the function of Leydig cells in prepubertal male rats by disrupting mitofusin 1-mediated mitochondrial function by Hang Lin, Ming Su, He Zhu, Yang Yu, Jianmin Sang, Yiyan Wang, Qiqi Zhu, Yang Zhu, Xiaoheng Li, Xingwang Li, Ren-Shan Ge, Huitao Li

“…The findings from in vivo experiments showed that CPA reduced serum testosterone and progesterone levels at 1 mg/kg/day. The qPCR and Western blotting analyses revealed significant alterations in the expression of genes and proteins pertinent to PLC function, such as Scarb1, Star, Cyp11a1, and Cyp17a1. …”
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Impact of Financial Literacy on Saving Decisions: Insight from Aseman Project Operated by Barkat Foundation by VAhid Moghadam, Hadi Amiri, Sahar Baharipour, Fatemeh Bagheri, Parisa GHasemian

“…For the variable of saving behavior, the Q-squared value was a strong value of 0.41.The path coefficients and significance levels revealed several key relationships: 1) Saving knowledge had a significant positive effect on saving attitude with a path coefficient of 0.53 (p<0.05). …”
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Inflammatory adhesion mediates myocardial segmental necroptosis induced by mixed lineage kinase domain-like protein in acute myocardial infarction by Lijiang Wei, Naifu Wan, Wentong Zhu, Chenchen Liu, Zeyu Chen, Wuwei Rong, Lujun Zhang, Meifeng Xie, Yueqi Qin, Ting Sun, Qing Jing, Ankang Lyu

“…The role of MLKL in regulating myocardial morphological necroptosis was evaluated using immunofluorescence staining, flow cytometry, qRT-PCR, Western blot, CCK-8 assay, and ELISA. …”
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Lipopolymers as the Basis of Non-Viral Delivery of Therapeutic siRNA Nanoparticles in a Leukemia (MOLM-13) Model by Panadda Yotsomnuk, Amarnath Praphakar Rajendran, Daniel Nisakar Meenakshi Sundaram, Luis Carlos Morales, Cezary Kucharski, Mohammad Nasrullah, Wanwisa Skolpap, Xiaoyan Jiang, Spencer B. Gibson, Joseph Brandwein, Hasan Uludağ

“…The quantitative analysis of gene silencing performed by qPCR as well as immunostaining/flow cytometry indicated significant reduction in both FLT3 expression and FLT3 protein after specific siRNA delivery. …”
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