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4281
Sport values and sustainability?
Published 2025-01-01“…To develop values that serve sustainability, stakeholders like coaches, PE teachers, and sports programs coordinators need to (1) consider sports characteristics and the way they orient the climate of practice, (2) be aware of the role of value socialization agents, and (3) pay attention to the norms and values (they make) salient during sport practice. References Hong, Q. N., Fàbregues, S., Bartlett, G., Boardman, F., Cargo, M., Dagenais, P., ... & Pluye, P. (2018). …”
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4282
Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy
Published 2025-06-01“…Neurobehavioral function was assessed using the Bederson score, and brain tissues were analyzed using hematoxylin and eosin (H&E) staining, TUNEL staining, and RT-qPCR for apoptosis-related genes. Results: The CP group exhibited significant neurobehavioral deficits and increased apoptosis. …”
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4283
Transcriptome analysis of 3D4/21 cells expressing CSFV NS4B
Published 2025-02-01“…Twelve DEGs were selected and further verified by RT-qPCR. Enrichment analyses of GO annotations and KEGG pathways revealed that these DEGs were associated with endocytosis, autophagy, cell adhesion, transport, immune response, apoptosis and so on. …”
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4284
The azimuthal correlation between the leading jet and the scattered lepton in deep inelastic scattering at HERA
Published 2024-12-01“…A measurement of jet cross sections in the laboratory frame was made in a fiducial region corresponding to photon virtuality $$10 {\,\text {Ge}\hspace{-0.66666pt}\text {V}}^2< Q^2 < 350 {\,\text {Ge}\hspace{-0.66666pt}\text {V}}^2$$ 10 Ge V 2 < Q 2 < 350 Ge V 2 , inelasticity $$0.04< y < 0.7$$ 0.04 < y < 0.7 , outgoing lepton energy $$E_e > 10 {\,\text {Ge}\hspace{-0.66666pt}\text {V}}$$ E e > 10 Ge V , lepton polar angle $$140^\circ< \theta _e < 180^\circ $$ 140 ∘ < θ e < 180 ∘ , jet transverse momentum $$2.5 {\,\text {Ge}\hspace{-0.66666pt}\text {V}}< p_\textrm{T,jet} < 30 {\,\text {Ge}\hspace{-0.66666pt}\text {V}}$$ 2.5 Ge V < p T,jet < 30 Ge V , and jet pseudorapidity $$-1.5< \eta _\textrm{jet} < 1.8$$ - 1.5 < η jet < 1.8 . …”
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4285
Identification of Adulterated Deer Blood Crystals in Traditional Chinese Medicine Based on MALDI-TOF MS Peptide Fingerprints Technique
Published 2025-01-01“…Firstly, Skyline software was used to predict candidate peptides by identifying the theoretical characteristic peptides for each species, and the mass spectrometry data of the tryptic digests were collected using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometer (UPLC-Q-TOF MS). Then, the mass spectrometry data were combined with UNIFI software and Uniprot database to accurately select a set of characteristic peptides that can reflect the differences between the target proteins, which was achieved by examining the presence of candidate characteristic peptides in the samples, as well as considering parameters such as the response, missed cut rate, and detection rate. …”
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4286
circICMT upregulates and suppresses the malignant behavior of bladder cancer
Published 2025-02-01“…Method: Differentially expressed circRNAs were identified from RNA sequencing data and circICMT was identified as a new candidate circRNA. qRT-PCR and sanger sequencing were used to detect the expression of circICMT in bladder cancer tissue specimens. …”
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4287
Primer estudio piloto para el tamizaje neonatal de linfopenias T y B en Colombia
Published 2024-12-01“…Se extrajo el ADN y se midieron los niveles de TREC y KREC por qPCR. Resultados. En los seis pacientes con sospecha de linfopenia se encontraron niveles muy bajos o indetectables de TREC. …”
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4288
Noncoding RNA profiling in omentum adipose tissue from obese patients and the identification of novel metabolic biomarkers
Published 2025-02-01“…Subsequent analyses included functional annotation with GO and KEGG pathway mapping, validation via real-time quantitative polymerase chain reaction (qRT‒PCR), the exploration of protein‒protein interactions (PPIs), and the identification of key regulatory genes through network analysis.ResultsThe results indicated that, compared with those in healthy individuals, various lncRNAs, circRNAs, and miRNAs were significantly differentially expressed in obese subjects. …”
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4289
Crocin improves post-thaw quality, fertility-associated gene expression and fertilization potential of buffalo bull sperm
Published 2023-11-01“…Expression levels of sperm acrosome-associated SPACA3, DNA condensation-related PRM1, anti- apoptotic BCL2, pro-apoptotic BAX, and oxidative stress-associated ROMO1 genes were evaluated through qPCR. The in vivo fertility of semen doses containing 1mM crocin was compared with control. …”
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4290
Comparative evaluation of antifungal susceptibility testing methods of invasive Candida species and detection of FKS genes mutations in caspofungin intermediate and resistant isola...
Published 2025-01-01“…Molecular characterization of the FKS gene demonstrated that FKS1 mutations V47I, V52K, V56T, D57S, L62F, I71Y, I71Q in the HS1 region, and G7S, P11H mutations in the HS2 region were associated with increased caspofungin MIC values (16 µg/ml). …”
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4291
Pre-sowing treatment of acorns with gibberellic acid and N-hexanoyl-L-homoserine lactone induced changes in growth and hormonal balance of Quercus robur L. seedlings
Published 2024-12-01“…The dynamics and distribution of endogenous cytokinins, abscisic (ABA), indole-3-acetic (IAA), gibberellic (GA3 and GA4) and salicylic (SA) acid in 47-day-old seedlings' organs of Q. robur were analysed using HPLC-MS. Priming with GA3 solution resulted in 85.8% of acorn germination, surpassing the control by 24.6%, whereas priming with C6-HSL solution led to 93.4% germination, surpassing the control by 32.2%. …”
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4292
Clinical Performance of MAGLUMI Diagnostic Tests for the Automated Detection of Dengue Virus
Published 2025-01-01“…Dengue-confirmed and excluded samples, validated by qPCR, were subjected to testing with MAGLUMI Denv NS1 in accordance with the manufacturer’s instructions. …”
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4293
The impact of uric acid on acute coronary syndrome prognosis in elderly patients
Published 2025-12-01“…Kaplan-Meier curves demonstrated a higher cumulative incidence of MACE during long-term follow-up in the Q4 group (Log-rank p < 0.05). Cox regression analysis revealed an independent correlation between UA levels and an increased risk of MACE (HR 1.002, 95%CI 1.000–1.003, p = 0.021). …”
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4294
Comprehensive analysis of mRNA and miRNA differential expression profiles in the hypothalamus-pituitary-gonadal axis in laying and broodiness period of Wanxi white geese
Published 2025-01-01“…Six DEGs and four DEMs were randomly selected for real-time fluorescence quantitative reverse transcription PCR (RT-qPCR). The results showed that the transcriptome sequencing data were accurate and reliable. …”
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4295
Suberosin attenuates rheumatoid arthritis by repolarizing macrophages and inhibiting synovitis via the JAK/STAT signaling pathway
Published 2025-01-01“…Methods Tumor necrosis factor-alpha (TNF-α) induced inflammation in RA-derived fibroblast-like synoviocytes (RA-FLS), and the expression of proinflammatory mediators was assessed using q-RT PCR and ELISA after treatment with various SBR concentrations. …”
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4296
Economic Evaluation of Robotic-assisted Radical Prostatectomy: A Systematic Review and Meta-analysis
Published 2025-02-01“…Heterogeneity was assessed using the Q test and I2 statistic. Key findings and limitations: Thirteen studies with 17 comparisons, ten from high-income (HICs) and three from middle-income (MICs) countries, were included. …”
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4297
The effect of green management on sustainable marketing orientation in small and medium industrial businesses: the mediating role of social legitimacy
Published 2024-05-01“…The new CVPAT index, instead of the Stone-Geisser Q2 index (>0.0), has been used. The new HTMT index also evaluates the divergent validity of the structural model. …”
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4298
Integrating rapamycin with novel PI3K/Akt/mTOR inhibitor microRNAs on NOTCH1-driven T-cell acute lymphoblastic leukemia (T-ALL)
Published 2024-07-01“…Gene and miRNA expression levels were assessed using quantitative real-time PCR (qRT-PCR). Two miRNAs exhibiting down-regulation in both clinical samples and Jurkat cells were transfected to the Jurkat cell line to investigate their impact on target gene expression. …”
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4299
Surveillance of tick-borne encephalitis virus foci in Slovakia: A seroprevalence study in ruminants combined with virus detection in ticks
Published 2025-01-01“…Overall, 2,534 ticks (2,528 Ixodes ricinus, 3 Dermacentor reticulatus, 2 Dermacentor marginatus, 1 Haemaphysalis concinna) from 7 collection sites were pooled and analysed by RT-qPCR, resulting in estimated prevalence of 2.86 % (ranging from 0.31 to 8.72 % at different sites). …”
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4300
Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Published 2018-01-01“…Cardiomyocytes were derived from hiPS cells that were generated from two healthy donors. qPCR and patch clamp techniques were used for the study. …”
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