FadA antigen of Fusobacterium nucleatum: implications for ceRNA network in colorectal cancer and adenomatous polyps progression by Sama Rezasoltani, Elahe Shams, Moein Piroozkhah, Yaser Aidi, Mehdi Azizmohammad Looha, Parmida Bagheri, Roudabeh Behzadi Andouhjerdi, Amir Sadeghi, Leili Rejali, Ehsan Nazemalhosseini-Mojarad

“…Subsequently, the mRNA, miRNA, and lncRNA expression levels were examined in intestinal biopsy specimens from 30 healthy controls, 30 Adenoma patients, and 30 cases of CRC stage I using qRT-PCR. Results Elevated expression levels of FadA, ANXA2, hsa-let-7a-2, and LINC00460 were observed in CRC specimens, followed by AP cases, in comparison to samples from normal individuals. …”
Get full text

Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos by Yuxin Chen, Ziwen Yang, Zhiqiang Guo, Lemei Zhan, Kenneth B. Storey, Danna Yu, Jiayong Zhang

“…The present study quantified the transcript levels of mitochondria protein-coding genes (mtPCGs) using quantitative real-time polymerase chain reaction (<i>RT-qPCR</i>) in samples of larvae of three dragonfly species (<i>A. parthenope</i>, <i>E. elegans</i>, and <i>G. confluens</i>) under different levels of CPF stress. …”
Get full text

Dihydrotanshinone I enhanced BRAF mutant melanoma treatment efficacy by inhibiting the STAT3/SOX2 signaling pathway by Xing Luo, Yi Duan, Jinwei He, CongGai Huang, Jun Liu, Yifan Liu, Mengdei Xu, Qiong Dai, Zhihui Yang

“…In this study, we investigated the effect of dihydrotanshinone I on the MAPK pathway inhibitor resistance of BRAF mutant malignant melanoma.MethodAfter treating A375, A375R, and A2058 cells with DHT or a combination of DHT and BRAF/MEK inhibitors, WB and Real-Time RT-qPCR were used to confirm the activation of the MAPK and STAT3/SOX2 pathways. …”
Get full text

Sevoflurane Exacerbates Cognitive Impairment Induced by Aβ1–40 in Rats through Initiating Neurotoxicity, Neuroinflammation, and Neuronal Apoptosis in Rat Hippocampus by Yue Tian, Ke-yan Chen, Li-dan Liu, Yun-xia Dong, Ping Zhao, Shan-bin Guo

“…Protein and mRNA expressions were executed by immunohistochemical (IHC) staining, Western blotting, and qRT-PCR. Results. Compared with the NS-treated group, sevoflurane only caused cognitive impairment and increased the level of Aβ1–42 of the brain in the Aβ-treated group. …”
Get full text

PD-L1 positive platelets mediate resistance to immune checkpoint inhibitors in patients with colorectal cancer by Jiacheng Li, Jia Liu, Shifeng Yang, Yu Xia, Qingzhe Meng, Biying Sun, Yansong Liu, Bin Zhao, Jiaqi Jin, Hui Xu, Lihong Wang, Pengxia Zhang, Zhuoxin Cheng

“…Methods The functional depletion effects of PD-L1 positive platelets on TME and immune cells were measured via western blotting, immunofluorescence staining, qRT-PCR, ELISpot and flow cytometry. In vivo, CD274 knockout (KO), CD8a KO, platelet-specific KO (PF4-Cre-Hsp90b1flox/flox) mouse models and a subcutaneous tumour model treated with aspirin and PD-L1 mAb were established in C57BL/6 N mice. …”
Get full text

Congenital Zika Syndrome: Insights from Integrated Proteomic and Metabolomic Analysis by Leticia Gomes-de-Pontes, Lucila Akune Barreiros, Lillian Nunes Gomes, Ranieri Coelho Salgado, Sarah Maria da Silva Napoleão, Paulo V. Soeiro-Pereira, Saulo Duarte Passos, Antonio Condino-Neto

“…EVs were isolated from CZS patient samples and control groups using Izon qEV size-exclusion chromatography columns. <b>Results:</b> The analyzed EVs presented distinct molecular profiles in patients with CZS. …”
Get full text

The necroptosis-related lncRNA ENSG00000253385.1 promotes the progression of esophageal squamous cell carcinoma by targeting the miR-16-2-3p/VDAC1 axis by Xiaoyang Duan, Jian Shi, Ran Hou, Yajie Huang, Chunyan Wang, Huazhen Du

“…FISH and quantitative real-time polymerase chain reaction (qRT‒PCR) were used to detect gene expression in ESCC tissues and cells. …”
Get full text

Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep by João Henrique Barbosa Toscano, Cintia Hiromi Okino, Isabella Barbosa dos Santos, Luciana Aparecida Giraldelo, Marei Borsch von Haehling, Sérgio Novita Esteves, Ana Carolina de Souza Chagas

“…RNA was extracted from tissue and blood samples for relative quantification of innate immune-related genes by RT-qPCR. For the abomasal fundic mucosa, increased TNFα and IL1β expression levels (P<0.05) were found in the susceptible animals, while resistant animals had IL33 superiorly expressed (P<0.05). …”
Get full text

RETRACTED ARTICLE: Bone mesenchymal stem cell-derived exosomal microRNA-7-5p inhibits progression of acute myeloid leukemia by targeting OSBPL11 by Duanfeng Jiang, Xin Wu, Xiaoying Sun, Wei Tan, Xin Dai, Youbang Xie, Ashuai Du, Qiangqiang Zhao

“…Methods Dysregulated miR-7-5p in AML patients was identified using qRT-PCR and its clinical significance was explored. …”
Get full text

Branched-chain amino acids levels associated with risk of erectile dysfunction: A Mendelian randomization analysis by Yijian Deng, Liying Zeng, Yigui Lai, Shuai Ji, Baizhao Peng, Hanqi Lu, Ming Wang, Hiu Yee Kwan, Qi Wang, Xiaoshan Zhao

“…The primary method used to assess causal associations was the inverse variance-weighted (IVW) method, supplemented by MR-Egger, weighted median, and simple median analyses. Cochrane's Q test was utilized to evaluate heterogeneity within the results, while the MR-Egger intercept test was utilized to evaluate the Level pleiotropy. …”
Get full text

GPR137-RAB8A activation promotes ovarian cancer development via the Hedgehog pathway by Chao Tang, Lin Li, Chongying Zhu, Qiang Xu, Zihao An, Shouying Xu, Chao Lin

“…The biological effects of GPR137-RAB8A-Hedgehog(HH) were investigated using in vitro and in vivo models and methods including qRT-PCR, RNA stability assay, RNA immunoprecipitation assay, GLI-luciferase reporter assay, nucleo-cytoplasmic separation assay, membrane-cytoplasmic separation assay, western blot, co-immunoprecipitation, immunofluorescence staining, cell counting kit-8 assay, wound healing assay, matrigel invasion assay, colony formation assay, xenografts assay, in situ transplantation tumor model of ovarian cancer in nude mice, and immunohistochemistry staining. …”
Get full text

Altered Left Ventricular Ion Channel Transcriptome in a High-Fat-Fed Rat Model of Obesity: Insight into Obesity-Induced Arrhythmogenesis by Reza Ashrafi, Marianne Yon, Lucy Pickavance, Joseph Yanni Gerges, Gershan Davis, John Wilding, Kun Jian, Henggui Zhang, George Hart, Mark Boyett

“…Left ventricle samples were removed at 8 weeks and mRNA expression of ion channels and other molecules was measured using qPCR. Obese rats had significant upregulation of Cav1.2, HCN4, Kir2.1, RYR2, NCX1, SERCA2a, and RYR2 mRNA and downregulation of ERG mRNA. …”
Get full text

TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis by Lin Wang, Diqing Ouyang, Lin Li, Yunchuan Cao, Yingwen Wang, Nina Gu, Zhaosi Zhang, Zhao Li, Shuang Tang, Hui Tang, Yuan Zhang, Xiaochuan Sun, Jin Yan

“…Various techniques were used to assess the role of DAM-like cells in TBI and the effects of glycolysis on DAM-like cells, including RT‒qPCR, immunofluorescence assays, behavioural tests, extracellular acidification rate (ECAR) tests, Western blot analysis, cell magnetic sorting and culture, glucose and lactate assays, and flow cytometry. …”
Get full text

Plasma prolactin axis shift from placental to pituitary origin in late prepartum mice by Taku James Sairenji, Shinnosuke Masuda, Yuya Higuchi, Mitsue Miyazaki, Hiroyuki Yajima, Oh Kwan Ee, Yuki Fujiwara, Takuya Araki, Noriaki Shimokawa, Noriyuki Koibuchi

“…The level of Prl3b1 mRNA was determined by RT-qPCR in the placenta and remained stable until parturition, implying that PRL3B1 peptide production or secretion was suppressed. …”
Get full text

Impaired hippocampal neurogenesis associated with regulatory ceRNA network in a mouse model of postoperative cognitive dysfunction by Jingrun Lin, Xiaoqiu Zhu, Xuan Li, Yu Hong, Yaohui Liang, Siqi Chen, Chenzhuo Feng, Lin Cao

“…Key findings were validated by RT-qPCR and immunofluorescence. The Connectivity Map (CMap) database was queried to predict potential POCD treatments. …”
Get full text

Vaginal Dysbiosis in Infertility: A Comparative Analysis Between Women with Primary and Secondary Infertility by Iliana Alejandra Cortés-Ortíz, Gustavo Acosta-Altamirano, Rafael Nambo-Venegas, Jesús Alejandro Pineda-Migranas, Oscar Giovanni Ríos-Hernández, Eduardo García-Moncada, Alejandra Yareth Bonilla-Cortés, Mónica Sierra-Martínez, Juan Carlos Bravata-Alcántara

“…Methods: Samples were obtained through cervical swabs, and microorganism identification was performed using qPCR techniques. Results: Analysis revealed a positive correlation between increased age and the likelihood of primary infertility, as well as a negative correlation with secondary infertility. …”
Get full text

Bifidobacterium animalis BD400 protects from collagen-induced arthritis through histidine metabolism by Yang Yang, Yang Yang, Qing Hong, Qing Hong, Xuehong Zhang, Zhenmin Liu, Zhenmin Liu

“…The expression of intestinal barrier protein was analyzed by qPCR to evaluate the intestinal barrier function. …”
Get full text

Heterogeneous occurrence of evergreen broad-leaved forests in East Asia: Evidence from plant fossils by Jiagang Zhao, Shufeng Li, Jian Huang, Wenna Ding, Mengxiao Wu, Tao Su, Alexander Farnsworth, Paul J. Valdes, Linlin Chen, Yaowu Xing, Zhekun Zhou

“…Paleoclimate simulation results suggest that the precipitation of wettest quarter (PWetQ, mm) exceeding 600 mm is crucial for the occurrence of EBLFs. …”
Get full text

Activation of Melanocortin Receptors MC1 and MC5 Attenuates Retinal Damage in Experimental Diabetic Retinopathy by S. Rossi, R. Maisto, C. Gesualdo, M. C. Trotta, F. Ferraraccio, M. K. Kaneva, S. J. Getting, E. Surace, F. Testa, F. Simonelli, P. Grieco, F. Merlino, M. Perretti, M. D’Amico, C. Di Filippo

“…Occludin progressively decreased in the retina of mice developing retinopathy. qPCR of murine retina revealed expression of two MC receptors, Mc1r and Mc5r. …”
Get full text

Knockdown of PDPN in astrocytes reduces hippocampal inflammation in T2DM mice by Xiaohang Wang, Xiaohang Wang, Xiaohang Wang, Qianqian Wang, Zhensheng Cai, Chengming Ni, Huan Wang, Hui Liu, Yusong Zhao, Jinbang Wang, Subo Liu, Xueying Wang

“…Our study aims to reveal its role in T2DM-induced hippocampal inflammation in VaD.MethodsFirstly, we will validate the expression of the Pdpn gene in T2DM astrocytes via qPCR and Western blot. Subsequently GFAP-specific promoter adeno-associated virus(AAV)carrying interfering sequence was used to knockdown the key gene in astrocytes of T2DM mice. …”
Get full text