Urinary exosomal miRNA-451a can be used as a potential noninvasive biomarker for diagnosis, reflecting tubulointerstitial damage and therapeutic response in IgA nephropathy by Qiong Zhang, Yan Zhao, Yankun Luo, Songjia Guo, Haizhu Hou, Xiaoli Han, Yun Zhou

“…The expression of miR-451a within exosomes was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). uE miR-451a was significantly upregulated in patients with IgAN compared to non-IgAN and HCs. …”
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Analysis of the CHS Gene Family Reveals Its Functional Responses to Hormones, Salinity, and Drought Stress in Moso Bamboo (<i>Phyllostachys edulis</i>) by Shiying Su, Xueyun Xuan, Jiaqi Tan, Zhen Yu, Yang Jiao, Zhijun Zhang, Muthusamy Ramakrishnan

“…Notably, <i>PeCHS14</i> consistently maintained high expression levels, suggesting its key role in stress response mechanisms. qRT-PCR analysis further validated the expression differences in five <i>PeCHS</i> genes under GA and ABA treatments. …”
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The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine by Shukui Zhou, Ying Wang, Kaile Zhang, Nailong Cao, Ranxing Yang, Jianwen Huang, Weixin Zhao, Mahbubur Rahman, Hong Liao, Qiang Fu

“…Then, acellular matrix was transplanted onto subcutaneous tissue for 1 week or 3 weeks; H&E staining and immunohistochemical analysis of CD68 expression and quantitative real-time PCR (qPCR) were performed to evaluate the immunogenicity and biocompatibility. …”
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Prevalence of asymptomatic Leishmania donovani infection and associated factors in Ethiopia: a systematic review and meta-analysis by Habtamu Belay, Adugna Abera, Esayas Aklilu, Tesfahun Bishaw, Ayinalem Alemu, Geremew Tasew, Berhanu Erko

“…Study heterogeneity assessed using Chi-square (χ²)-based Q test (p < 0.1) and I² subgroup analysis and meta-regression conducted with significance set at p < 0.05. …”
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miR-155 Predicts Long-Term Mortality in Critically Ill Patients Younger than 65 Years by Frank Tacke, Martina E. Spehlmann, Mihael Vucur, Fabian Benz, Mark Luedde, David Vargas Cardenas, Sanchari Roy, Sven Loosen, Hans-Joerg Hippe, Norbert Frey, Christian Trautwein, Alexander Koch, Christoph Roderburg, Tom Luedde

“…Concentrations of circulating miR-155 were determined in 218 critically ill patients and 76 healthy controls. Results. By using qPCR, we demonstrate that miR-155 serum levels are elevated in patients with critical illness when compared to controls. …”
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Comment acquérir une compétence d’oral spontané en situation d’apprentissage institutionnalisée by Günter Schmale

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Early Treatment with Empagliflozin and GABA Improves β-Cell Mass and Glucose Tolerance in Streptozotocin-Treated Mice by Caroline Daems, Sophie Welsch, Hasnae Boughaleb, Juliette Vanderroost, Annie Robert, Etienne Sokal, Philippe A. Lysy

“…Islets from EMPA-treated mice were also less subjected to ER (endoplasmic reticulum) stress and inflammation, as shown by qPCR analysis. Glucose homeostasis parameters and islet area/pancreas area ratio improved, as compared to diabetic controls, when T1D mice were treated for three weeks with GABA and EMPA. …”
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Functional differentiation of human dental pulp stem cells into neuron-like cells exhibiting electrophysiological activity by B. Pardo-Rodríguez, A. M. Baraibar, I. Manero-Roig, J. Luzuriaga, J. Salvador-Moya, Y. Polo, R. Basanta-Torres, F. Unda, S. Mato, Gaskon Ibarretxe, Jose Ramon Pineda

“…We (2) improved hDPSC neurodifferentiation by adding retinoic acid (RA) and potassium chloride (KCl) pulses for 21 or 60 days and characterized the results by immunofluorescence, digital morphometric analysis, RT-qPCR and electrophysiology. Results We found that neural markers like Nestin, GFAP, S100β and p75NTR were expressed differently in neurodifferentiated hDPSC cultures depending on the presence or absence of serum during the initial cell expansion phase. …”
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Tocilizumab modulates the activity of the classical and alternative complement pathways in rheumatoid arthritis patients by Iván Ferraz-Amaro, Iván Ferraz-Amaro, Sergio Santos-Concepción, Javier Castro-Hernández, Maria Vanesa Hernández-Hernández, Beatriz Tejera Segura, Cristina Luna, Esmeralda Delgado-Frias, Federico Díaz-González, Federico Díaz-González, Federico Díaz-González

“…The complement system has been implicated in the etiopathogenesis of RA.ObjectiveTo evaluate the effect of systemic IL-6 inhibition on complement pathways functional activity in RA patients treated with TCZ.DesingProspective non-interventional study.MethodsTwenty-seven RA patients included in the TOCRIVAR study who received TCZ (8mg/kg IV/q4w) were evaluated at baseline and at weeks 12, 24 and 52 of treatment. …”
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Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential? by Carla C. G. Pinheiro, Alessander Leyendecker Junior, Daniela Y. S. Tanikawa, José Ricardo Muniz Ferreira, Reza Jarrahy, Daniela F. Bueno

“…To evaluate whether greater osteogenic potential of the established mesenchymal stem cell strains was associated with an increase in the expression profile of neural crest genes, real-time qPCR was performed on the following genes: SRY-box 9, SRY-box 10, nerve growth factor receptor, transcription factor AP-2 alpha, and paired box 3. …”
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Genetic divergence of hot pepper lines (Capsicum spp.) in hybrid combinations by Šević Biljana, Cvikić Dejan, Slađana Savić, Tupajić Ivan, Ugirnović Milan, Bajagić Marija, Luković Kristina, Stojiljković Jelena

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Efecto del nivel de suplementación con harina de algodón sobre el consumo y el aumento de peso en bovinos en recría alimentados con silaje de sorgo by Jose L. Chiossone, O. Balbuena

“….-1 difirió (p<0,05) con el aumento del nivel de suplementación, pero no se detectan diferencias (p=0,12) como g kg-1 PV, aunque en ambos casos se verificó un efecto cuadrático (Q<0,05). El tratamiento testigo (P0) presentó un ADPV de 0,07 kg an-1 .-1, con un CS de 18,2 g kg-1 PV que se incrementó 31,3% al incluir 3 g kg-1 PV de HA. …”
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Lipid-encapsulated gold nanoparticles: an advanced strategy for attenuating the inflammatory response in SARS-CoV-2 infection by Sanjeevram Dhandapani, Yujeong Ha, Rongbo Wang, Tae Woo Kwon, Ik-Hyun Cho, Yeon-Ju Kim

“…This effect was mitigated with KGS-NE oral administration, as observed through H&E and qRT-PCR analysis. Biochemical analysis at various oral administration concentrations demonstrated that KGS-NE had no adverse effects on the kidney and liver. …”
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Keeping Active with Texting after Stroke (KATS): a single-arm feasibility and acceptability study of a behavioural intervention to promote community-based physical activity after s... by Gozde Ozakinci, Stephan U Dombrowski, Andrew Murphy, Albert Farre, Jenna Breckenridge, Jacqui H Morris, Linda A Irvine, Keith Jenkinson

“…Based on these findings, KATS will be further optimised before evaluating effectiveness in a randomised controlled trial.Trial registration number and protocol availability ISRCTN 13704805 https://www.hra.nhs.uk/planning-and-improving-research/application-summaries/research-summaries/keeping-active-with-texting-after-stroke-kats/Protocol available https://www.isrctn.com/ISRCTN13704805?q=13704805&amp;filters=&amp;sort=&amp;offset=1&amp;totalResults=1&amp;page=1&amp;pageSize=10…”
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Effectiveness of flipped classrooms in Chinese students of clinical medicine major undergoing clinical practice: a meta-analysis by Xinrui Sun, Xin Yuan, Linyu Zhang, Yi Zhang, Xue Xiao, Hanmin Liu, Fang Ma

“…Weighted mean differences (WMD) and 95% confidence interval (CI) were used to compare the differences in theoretical and skill scores between the flipped classrooms and traditional lecture groups. The Cochran Q test and the I2 statistic were used for heterogeneity analysis. …”
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Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus by Jiang Ye, Xiaowei Wu, Xiang Li, Yuting Zhang, Yuqing Li, Hui Zhang, Jie Chen, Yuyan Xiang, Yefan Xia, Hu Zhao, Zengdong Tan, Liang Guo, Xuan Yao

“…Through genome-wide association studies in Brassica napus accessions, we identify BnaA09.MYB52 transcription factor as a candidate gene underlying ZS11_qSCC.A09. Knocking out BnaMYB52 results in a 7.7%–8.2% reduction in seed coat content (SCC) but a 12.3%–13.4% increase in SOC, whereas overexpression of BnaA09.MYB52 leads to the opposite effects. …”
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Gut microbiota and kidney function in autosomal dominant polycystic kidney disease participants in Cameroon: a cross-sectional study by Inès Obolo Nwaga, Victorine Bandolo Nzana, Rhoda Nsen Bughe, Isaac Dah, Cheboh Cho-Fon, Maimouna Mahamat, Emmanuelle Ndjong, Aristide Nono, Jean Claude Mballa, Fon Abongwa Acho, Vicky Ama Moor, Wilfred Fon Mbacham, François Folefack Kaze

“…Microbial DNA extracted from stool specimens and amplified by qPCR was used to quantify Enterobacteriaceae, Bifidobacterium and Lactobacillus abundance. …”
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Cancer-associated fibroblast-derived COL17A1 promotes gemcitabine resistance and tumorigenesis in pancreatic cancer cells by interacting with ACTN4 by Rongyu Shi, Ning Zhang, Han Li, Hu Zhong, Chengcheng Zhong, Wei Du, Xi Yang

“…Levels of COL17A1 and Actinin Alpha 4 (ACTN4) were measured by using qRT-PCR and western blotting. Functional analyses were conducted using MTT, 5-ethynyl-2ʹ-deoxyuridine, transwell, and sphere formation assays, respectively. …”
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Combinatorial functionomics identifies HDAC6-dependent molecular vulnerability of radioresistant head and neck cancer by Sharon Pei Yi Chan, Celestia Pei Xuan Yeo, Boon Hao Hong, Evelyn Mui Cheng Tan, Chaw Yee Beh, Eugenia Li Ling Yeo, Dennis Jun Jie Poon, Pek Lim Chu, Khee Chee Soo, Melvin Lee Kiang Chua, Edward Kai-Hua Chow

“…Regulatory roles of HDAC6/SP1 axis were investigated using immunoprecipitation, gel shift and ChIP-qPCR assays. Comparative transcriptomic analyses were employed to determine the prognostic significance of targeting HDAC6. …”
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Pleiotropic Effects of a KCNQ1 Variant on Lipid Profiles and Type 2 Diabetes: A Family-Based Study in China by Xiaowen Wang, Junhui Wu, Yao Wu, Mengying Wang, Zijing Wang, Tao Wu, Dafang Chen, Xun Tang, Xueying Qin, Yiqun Wu, Yonghua Hu

“…The genetic variant rs2237895, located in the Potassium Voltage-Gated Channel Subfamily Q Member 1 (KCNQ1) gene, has been replicated to be associated with type 2 diabetes mellitus (T2DM) susceptibility, but the relationship with lipids is conflicting. …”
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