The Frequency of <i>DPYD</i> c.557A>G in the Dominican Population and Its Association with African Ancestry by Mariela Guevara, Carla González de la Cruz, Fernanda Rodrigues-Soares, Ernesto Rodríguez, Caíque Manóchio, Eva Peñas-Lledó, Pedro Dorado, Adrián LLerena

“…<b>Methods:</b> For this study, 196 healthy volunteers from the Dominican Republic were genotyped for <i>DPYD</i> variants by qPCR, and individual genomic ancestry analysis was performed in 178 individuals using 90 informative ancestry markers. …”
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PARALELO ENTRE LA ACTIVIDAD Y LA SUSTANCIA ADICTIVA DESDE UN ENFOQUE SOCIAL Y SUS POSIBLES TRATAMIENTOS by Alan Herzberg, Viviana Messina, Diego Moreno, Natalia Olmedo, Romina Pitronello, Francisca Roman

“…El tema de las adicciones ha sido abordado históric amente por la literatura desde múltiples puntos de vista, sin embargo en esta investigación bibliográfica nuestra intenci ón es dimensionar las teorías sobre el origen de la s adicciones, considerando la complementariedad teórica para la q ue se presta el tema, permitiendo un paralelo con l as terapias psicoanalíticas y la visión freudiana. …”
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کارایی مدل ویکور در پهنه‌بندی خطر زمین‌لغزش در حوضه آبخیز سد گلال استان ایلام by فتح الله نادری, بهروز ناصری, نعمت اله بسطامی

“…سپس بر پایه الگوریتم بهینه‌سازی ویکور، درجه سودمندی و پشیمانی انتخاب گزینه‌های برتر (زیر حوضه‌ها)، مشخص و در پایان با محاسبه میزان شاخص بهینه ویکور (Q)، زیر حوضه‌ها برحسب درجه حساسیت پذیری به زمین‌لغزش در سه کلاس کیفی حساسیت­پذیری کم، متوسط و زیاد طبقه‌بندی شدند. …”
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Comparative evaluation of various DNA extraction methods and analysis of DNA degradation levels in commercially marketed Chestnut rose juices and beverages by Yongchao Ren, Yunlong Ma, Yanqi Li, Yun Song, WeiWei Zhao, Xuncai Huang, Danmin Yu, Jian Li, Zuogang Xu, Wenjun Zhao

“…Methods The quantity and quality of the extracted DNA were assessed using NanoDrop One spectrophotometer, gel electrophoresis, and real-time polymerase chain reaction (real-time PCR or qPCR) assays. An assessment was conducted on the processing time, labor intensity, and cost associated with each approach. …”
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Integrative analysis of metabolite and transcriptome reveals the biosynthetic pathway and candidate genes for iridoid glycoside biosynthesis in Neopicrorhiza scrophulariiflora (Pen... by Ke Rao, Ke Rao, Siyu Liu, Siyu Liu, Xiaohui Tang, Guofu Jia, Shaohua Yang, Chaoxiang Ren, Chaoxiang Ren, Jin Pei, Jin Pei

“…Transcriptome analysis revealed the expression patterns of differentially expressed genes, and metabolomic analysis revealed the distribution characteristics of metabolites in different tissues of N. scrophulariiflora. Through qRT-PCR validation, we found that three NsF3H/NsF3D genes, four NsUGD/NsUPD genes, one Ns2HFD gene, and three NsSQM genes may participate in the iridoid biosynthesis pathway. …”
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Unveiling new therapeutic horizons in rheumatoid arthritis: an In-depth exploration of circular RNAs derived from plasma exosomes by Guoqing Li, Hongyi Chen, Jiacheng Shen, Yimin Ding, Jingqiong Chen, Yongbin Zhang, Mingrui Tang, Nan Xu, Yuxuan Fang

“…In addition, the morphology and size of exosomes and the expression of CD9 and CD81 verified the successful extraction of exosomes. The qPCR analysis of plasma exosomes in TNFi patients found that the expression of hsa-circ0002715 was higher than that in patients who didn’t reach ACR20, and the expression of hsa-circ0001946 was lower than that in patients who didn’t reach ACR20. …”
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DKK3 promotes renal fibrosis by increasing MFF-mediated mitochondrial dysfunction in Wnt/β-catenin pathway-dependent manner by Jianling Song, Yanxia Chen, Yan Chen, Minzi Qiu, Wenliu Xiang, Ben Ke, Xiangdong Fang

“…Western blotting, IHC, and qRT-PCR were performed to detect molecular expression levels. …”
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Effects of biochar on the chemical properties of soils and the volume of wood in a plantation of Acacia mangium Willd in the Colombian Orinoquía (highlands) by Giovanni Reyes-Moreno, Aquiles Enrique Darghan, Carlos Rivera-Moreno

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Inhibition of complement system-related gene ITGB2 attenuates epithelial–mesenchymal transition and inflammation in diabetic nephropathy by Jun Peng, Wenqi Zhao, Lu Zhou, Kun Ding

“…Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and western blotting were utilized to detect the ITGB2 expression. …”
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Low Serum miR-607 Level as a Potential Diagnostic and Prognostic Biomarker in Patients of Pancreatic Ductal Adenocarcinoma: A Preliminary Study by Dawei Jiang, Xiangfei Yuan, Jianqi Ni, Lan Shen, Min Cai, Liu Xu

“…The expression levels of miR-607 were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). The correlation between miR-607 expression and clinical characteristics was analyzed by the Chi-square test. …”
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Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in mice by Xiaoyan Duan, Xiaoyan Duan, Xiaoyan Duan, Xiaoyan Duan, Rilige Wu, Jianbo Li, Jianbo Li, Zeya Li, Yanqi Liu, Ping Chen, Bangmao Wang, Bangmao Wang, Bangmao Wang

“…The expression of the relevant factors in the blood supernatant of the mice was determined by ELISA. RT-qPCR and Western blotting were used to detect mRNA and protein expression of target genes. …”
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Appropriate Complementary Feeding Practice and Its Associated Factors among Mothers Who Have Children Aged between 6 and 24 Months in Ethiopia: Systematic Review and Meta-Analysis by Asrat Hailu Dagne, Shimeles Biru Zewude, Abenezer Melkie Semahegn

“…Data analysis was conducted using STATA version 11. Cochran (Q test) and I2 test were used to test the heterogeneity of the studies. …”
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Identification of Potential miRNA-mRNA Regulatory Network Contributing to Parkinson’s Disease by Xi Yin, Miao Wang, Wei Wang, Tong Chen, Ge Song, Yixuan Niu, Ziying Jiang, Zhongbao Gao, Zhenfu Wang

“…We further investigated the expression of genes in the miRNA-mRNA regulatory network in blood samples collected from PD patients and healthy donors via qRT-PCR. We identified 1505 upregulated and 1302 downregulated DEGs, and 77 upregulated and 112 downregulated DEmis were preliminarily screened from the GEO database. …”
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Correlation Between Inflammatory Markers and Pathogenic Bacteria in Children&rsquo;s Winter Respiratory Infections in Xinjiang by Wang L, Wang W, Wang J, Zhu L, Luo J

“…Using Indirect Immunofluorescence Antibody test(IFA), blood routine, C-reactive Protein (CRP), and High-sensitivity C-reactive Protein(hsCRP), we detected nine respiratory pathogens (Respiratory syncytial virus; Influenza A virus; Influenza B virus; Parainfluenza virus; Adenovirus; Mycoplasma pneumoniae; Chlamydia pneumoniae; Legionella pneumophila type 1; Rickettsia Q) in all patients and analyzed their distribution and correlation. …”
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Traces of Paleolithic expansion in the Nivkh gene pool based on data on autosomal SNP and Y chromosome polymorphism by V. N. Kharkov, N. A. Kolesnikov, L. V. Valikhova, A. A. Zarubin, A. L. Sukhomyasova, I. Yu. Khitrinskaya, V. A. Stepanov

“…The Nivkh haplogroup O2a1b1a2a-F238 is found among residents of China and Myanmar. The Q1a1a1-M120 line is represented among the Nivkhs, Koryaks, Evenks and Yukaghirs. …”
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Derivation and Verification of the Relationship between Ablation Index and Baseline Impedance by Zheng Cai, Sainan Li, Qi Zhang, Chenyuan Wang, Zhen Jin, Ming Fu, Shuai Zhang, Ming Liang, Zulu Wang, Yaling Han

“…Methods. (1) Keeping the AIs relatively constant, the lesion dimensions in different baseline impedances were studied. (2) According to Joule's law, Q = I2RT, keeping the current (I) unchanged, the powers corresponding to different baseline impedances can be obtained. …”
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Automated Generation of Multiple-Choice Questions for Computer Science Education Using Conditional Generative Adversarial Networks by Muhammad Shoaib, Ghassan Husnain, Nasir Sayed, Yazeed Yasin Ghadi, Masoud Alajmi, Ayman Qahmash

“…The model is trained on a carefully constructed dataset that includes material from more than 20 subareas in computer science, consisting of materials such as textbooks, online encyclopedias and Q&#x0026;A websites. Through rigorous evaluation using comprehensive performance metrics, including Question Relevance Score (QRS), Diversity Index (DI), and Difficulty Alignment Accuracy (DAA), we demonstrate the efficacy and robustness of our framework in generating high-quality MCQs. …”
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Mechanism of melanoma suppression by Prosopis juliflora derived alkaloids: Apoptosis induction and signaling pathway inhibition by Jasoda Choudhari, Snehal K. Nimal, Shridhar Chougule, Trupti Shinde, N.R. Dhatrak, Gopal C. Kundu, Rajesh N. Gacche

“…Mechanistic study of PJLME-AF was explored using ROS generation assay, apoptosis assays (Annexin V) assay, western blot (apoptotic protein, EMT markers, stem cell markers and signalling pathways), qPCR. In-vitro spheroid assay was performed to explored the PJLME-AF cytotoxic effects on tumour spheroid forming ability of melanoma cells. …”
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Identification of SETD4 as an Onco‐Immunological Biomarker Encompassing the Tumor Microenvironment, Prognoses, and Therapeutic Responses in Various Human Cancers by Yuyun Zhong, Ruiqi Wang, Zijie Huang, Zhaoting Hu, Bin Peng, Bin Chen, Liyue Sun

“…Furthermore, we investigated its association with cancer‐related pathways, the immune microenvironment, immunotherapy markers, and drug resistance signatures of chemotherapy. Additionally, qRT‐PCR was performed to validate SETD4 expression in clinical specimens. …”
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Identification and Analysis of Potential Immune-Related Biomarkers in Endometriosis by Yanan He, Jixin Li, Yanjun Qu, Liyuan Sun, Xibo Zhao, Han Wu, Guangmei Zhang

“…Then, we used quantitative real-time polymerase chain reaction (qRT-PCR) to verify the hub genes. Simultaneously, the Pearson correlation analysis and protein-protein interaction (PPI) analyses were used to indicate the potential mechanisms of TF-IRGs at the molecular level and obtain hub IRGs. …”
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