Relieving Effect of <i>Artemisia ordosica Krasch</i> Extract on DSS-Induced Colitis by Regulating Immunity, Antioxidant Function, Gut Microbiota, and Bile Acid Metabolism in Mice by Min Jiang, Xuekai Zhang, Xiao Jin, Binlin Shi, Yuanqing Xu, Zheqi Wang

“…In vivo studies demonstrated that AOE reduced DSS-induced colitis in mice by increasing the colon length, enhancing antioxidant enzyme activity, inhibiting inflammatory cell infiltration, suppressing the formation of TNF-α and IL-6, and reducing malondialdehyde (MDA) levels. qPCR analysis revealed that AOE reversed the down-regulation of <i>Claudin</i> mRNA expression, and altered the composition of cecal microbiota, thus mitigating DSS-induced colitis. …”
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Correlation between malondialdehyde level and FOXO3 and CASP3 mRNA expression changed in early-onset preeclampsia placenta by Ni Made Wiasty Sukanty, Febriana Catur Iswanti, Syarifah Dewi, Muhammad Faruqi, Alyssa Shafa Andiana, Ani Retno Prijanti

“…A total of 31 EOPE placentas and 31 normal term placentas were used to measure the concentration of malondialdehyde (MDA) and the relative mRNA expression of FOXO3 and CASP3 using the spectrophotometric and RT-qPCR methods. Results: There was no difference in MDA concentration (p = 0.580) and FOXO3 (p = 0.467) and CASP3 (p = 0.243) mRNA expression in the normal and EOPE groups. …”
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Effects of Ginsenoside Biopolymer Nanoparticles on the Malignant Behavior of Non-Small-Cell Lung Cancer by Weizheng Zhou, Chengliang Cai, Hui Shi, Hai Jin, Xiaowei Wang

“…Cell scratch test was used to determine the migration ability of H125 cells, and Western blotting was used to measure the expression level of PTEN in H125 cells; the expression level of miR-192 in H125 cells was measured via RT-qPCR, and the apoptosis level of H125 cells was detected by Tunel assay. …”
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A Cross-Sectional Trait Versus State Biomarker Analysis of Inflammatory Cytokines and miRNAs in Patients with Major Depressive Disorder by Christopher Rockson, Chandrashekaran Girish, Harivenkatesh Natarajan, Vikas Menon

“…We quantified the gene expressions of plasma miR-16, miR-132, and miR-1202 using Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). We estimated the total oxidant status and total antioxidant status levels in the serum by ELISA. …”
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A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats by Hong Ma, Guo-Ping You, Xuan-Pu Zhang, Xiang-Jiu Yang, Hua-Dong Lu, Yan-Ling Huang, Wen-Qiang Zhang

“…The expressions of adiponectin receptors (adipoR1/R2) in liver and skeletal muscle tissues were detected through western blotting or RT-qPCR, respectively. Results. Globular adiponectin alleviated the hepatic steatosis and increased insulin secretion. …”
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In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium by Shaoyuan Xu, Jie Li, Xiaoyan Chen, Beiyu Liu

“…Annexin IV and VEGF were analyzed by qRT-PCR (mRNA), western blot (proteins), and immunohistochemistry (proteins). …”
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Detector development for spin-echo SANS techniques using ZnS:Ag/6LiF and 6Li glass scintillators by Giacomo Mauri, G. Jeff Sykora, Gregory N. Smith, Steven R. Parnell, Robert M. Dalgliesh, Dirk Honecker, Sarah E. Mann, Erik M. Schooneveld, Nigel J. Rhodes

“…These are powerful means to extend the measurable momentum transfer (Q) and energy (E) range in neutron scattering measurements. …”
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Prevalence and pattern of rheumatic valvular heart disease in Africa: Systematic review and meta-analysis, 2015-2023, population based studies. by Seid Mohammed Abdu, Altaseb Beyene Kassaw, Amare Abera Tareke, Gosa Mankelkl, Mekonnen Belete, Mohammed Derso Bihonegn, Ahmed Juhar Temam, Gashaw Abebe, Ebrahim Msaye Assefa

“…The descriptive information for the study is presented in the table, and the quantitative results are presented in forest plots. The Cochrane Q test and I2 test statistic were used to test heterogeneity across studies. …”
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Omicron Positivity in Air of Hospital Settings Gathered COVID-19 Patients, Vaccinated/Unvaccinated Populations by Zeynep Ece Kuloğlu, Zeynep Bengi Eren, Bedirhan Haykar, Cansel Vatansever, Tayfun Barlas, Mert Ahmet Kuskucu, Gülen Güney-Esken, Füsun Can

“…In the cross-sectional part, we collected aerosol samples from clinical and public areas in the hospital. We performed qPCR and viral culture. In the pediatrics, outpatient clinic waiting room, where the children were without masks, and unvaccinated, 66% of the samples were positive for viral RNA. …”
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Effect of phenylbutazone administration on the enteroinsular axis in horses with insulin dysregulation by Kate L. Kemp, Jazmine E. Skinner, François‐René Bertin

“…Methods Randomized cross‐over study design, with horses assigned to treatment with phenylbutazone (4.4 mg/kg IV q24h) or placebo (5 mL 0.9% saline). On Day 9 of treatment, an OGT was conducted, followed by a 10‐day washout period, administration of the alternative treatment, and repetition of the OGT. …”
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Putative and pretreatment drug resistance mutations in reverse transcriptase gene among untreated chronic hepatitis B patients at Arifin Achmad Regional District Hospital, Riau, In... by Arfianti Arfianti, Fauzia Andrini Djojosugito, Maisaroh Maisaroh, Hendra Asputra, Dita Kartika Sari, Tubagus Odih Rhomdani Wahid

“…In addition, F221Y and N238H/Q mutations were frequently observed in genotype B, while V224 I was only found in patients infected with genotype C (p=0.000). …”
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Inhibiting METTLl3-ATG5 axis-mediated harmful autophagy in macrophages could help reduce airway epithelial inflammation and remodeling in COPD by Chen Guiyang, Xia Zhiwei, Zeng Xianyou, Liu Hanying

“…Methylated RNA immunoprecipitation-qPCR showed that METTL3 knockdown reduced m6A, and the actinomycin D assay suggested that METTL3 knockdown reduced ATG5 mRNA levels and lowered ATG5 mRNA stability. …”
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Two Novel CYP11B1 Gene Mutations in Patients from Two Croatian Families with 11β-Hydroxylase Deficiency by Katja Dumic, Tony Yuen, Zorana Grubic, Vesna Kusec, Ingeborg Barisic, Maria I. New

“…Sequencing of the CYP11B1 gene reveals that this patient is compound heterozygous for a novel, previously undescribed p.R141Q (c.422G>A) mutation in exon 3 and a p.T318R (c.953C>G) mutation in exon 5. …”
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Unraveling TGF-β1’s Role in Mediating Fibrosis and Cell Death in Feline Kidney Cells by Chanyanuch Intachat, Phongsakorn Chuammitri, Benjaporn Sornpet, Prapas Patchanee, Nawin Manachai, Kakanang Piyarungsri

“…A gene expression analysis was conducted using qPCR to calculate the relative gene expression, while the protein expression was assessed through Western blot analysis. …”
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Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells by Amir Hossein Aalami, Mohammad Mesgari, Amirhossein Sahebkar

“…MTT assays showed that the inhibitory concentration (IC50) of ZnO-NPs after 24 h was 11.16 μg/mL, 60.08 μg/mL, and 26.3 μg/mL on MCF7, MDA-MB-231, and HFF cells, respectively. The qRT-PCR results showed reduced expression of miR-155-5p, miR-203a-3p, and miR-223-3p when the MCF7 cells were treated with the IC50 concentration of ZnO-NPs (11.16 μg/mL). …”
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Effect of glyphosate on renal function: A study integrating epidemiological and experimental evidence by Lin Hu, Mingcong Chen, Xiaoran Xue, Mingyi Zhao, Qingnan He

“…The underlying mechanism of the association between glyphosate and renal function was explored by the real-time quantitative polymerase chain reaction (RT-qPCR) and non-targeted metabolomics analysis [embryos (0.75 hpf–96 hpf) were exposed to 90 μg/mL glyphosate]. 1170 participants were enrolled in the NHANES study. …”
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Oncostatin M and TLR-4 Ligand Synergize to Induce MCP-1, IL-6, and VEGF in Human Aortic Adventitial Fibroblasts and Smooth Muscle Cells by David Schnittker, Karen Kwofie, Ali Ashkar, Bernardo Trigatti, Carl D. Richards

“…When combined, LPS and OSM synergized to increase MCP-1, IL-6, VEGF protein, and mRNA expression as assessed by qRT-PCR, in both HAoAFs and HAoSMCs, while LPS-induced IL-8 levels were reduced. …”
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CircSPG21 ameliorates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells and mitigates intervertebral disc degeneration through the miR-217/SIRT... by Yongbo Zhang, Sheng Yang, Xuan You, Zhengguang Li, Liuyang chen, Rui Dai, Hua Sun, Liang Zhang

“…Interactions among circSPG21, miR-217, and the NAD-dependent protein deacetylase sirtuin-1 (SIRT1) were validated through dual-luciferase assays, RNA fluorescence in situ hybridization and RNA immune precipitation. β-Gal staining, EdU staining, Western blotting, JC-1 assays, cell cycle analysis, and quantitative reverse transcription PCR (RT‒qPCR) were used to examine the functions of these molecules in TBHP-induced senescent NPMSCs. …”
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Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1 by Adel, Eltoukhy, Yang, Jia, Imane, Lamraoui, M. A., Abo‑Kadoum, Omar, Mohammad Atta, Ruth, Nahurira, Junhuan, Wang, Yanchun, Yan

“…The pathways of microbial metabolism in diverse environments were significantly enriched among DEGs based on KEGG enrichment analysis. qRT‑PCR confirm the involvement of BPA degradation relevant genes in accordance with RNA Seq data. …”
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RETRACTED: A Synthetic Peptide Designed to Neutralize Lipopolysaccharides Attenuates Metaflammation and Diet-Induced Metabolic Derangements in Mice by Shireen Mohammad, Sura Al Zoubi, Sura Al Zoubi, Debora Collotta, Nadine Krieg, Nadine Krieg, Bianka Wissuwa, Bianka Wissuwa, Gustavo Ferreira Alves, Gareth S. D. Purvis, Gareth S. D. Purvis, Giuseppe Danilo Norata, Giuseppe Danilo Norata, Giuseppe Danilo Norata, Andrea Baragetti, Andrea Baragetti, Alberico Luigi Catapano, Alberico Luigi Catapano, Alberico Luigi Catapano, Egle Solito, Egle Solito, Elisabeth Zechendorf, Tobias Schürholz, Wilmar Correa-Vargas, Klaus Brandenburg, Sina M. Coldewey, Sina M. Coldewey, Massimo Collino, Muhammad M. Yaqoob, Lukas Martin, Lukas Martin, Christoph Thiemermann

“…The liver steatosis caused by feeding mice a HFD resulted in the activation of nuclear factor kappa light chain enhancer of activated B cells (NF-ĸB) (phosphorylation of inhibitor of kappa beta kinase (IKK)α/β, IκBα, translocation of p65 to the nucleus), expression of NF-ĸB-dependent protein inducible nitric oxide synthase (iNOS) and activation of the NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome, all of which were reduced by Peptide 19-2.5. …”
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