Molecular Mechanism During Mycelium Subculture Degeneration of <i>Volvariella volvacea</i> by Lidan Feng, Lujuan Wang, Yuanxi Lei, Jie Li, Fengyun Zhao

“…Thus, five generations of subcultured strains (M1–M5) were obtained after 20 months of mycelial subculturing, their production traits were determined, and transcriptomic analysis was performed using RNA-seq; the differentially expressed genes were verified via RT-qPCR. The results showed that as the number of subcultures increased, the diameter of the mycelium and biological efficiency gradually decreased; in addition, the time in which the primordium formed increased and the production cycle was lengthened, while strains M4 and M5 lacked the ability to produce fruiting bodies. …”
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Trend of Soil-Transmitted Helminths in Ethiopian Children: A Systematic Review and Meta-Analysis (2000-2018) by Getaneh Alemu, Feleke Mekonnen, Mezgebu Nega, Chalachew Muluneh

“…Heterogeneity between studies was assessed using the Cochran Q test and I2 test statistics based on the random effect model. …”
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Multispecies fisheries management of the Sultanate of Oman using bioeconomic model by Sachinandan Dutta, Majid Al Harthy, Saud M. Al Jufaili, Ibtisam Al Abri

“…Rastrelliger kanagurta exhibited the highest growth rate (r = 0.260), with similar catchability coefficients (q) between R. kanagurta (2.18E-05) and S. commerson (2.93E-05), and T. albacares (8.48E-06) and S. longiceps (8.41E-06). …”
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Bivalirudin exerts antiviral activity against respiratory syncytial virus-induced lung infections in neonatal mice by Zhuang Shihao, Tang Qiuyu, Chen Ping, Wang Chengyi, Liu Guanghua

“…Further, to delineate the role of micro RNAs, the qRT-PCR analysis was done, and the results displayed an increase in miR-136, miR-30b, and let-7i. …”
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miR-149 Alleviates Ox-LDL-Induced Endothelial Cell Injury by Promoting Autophagy through Akt/mTOR Pathway by Zhongsheng Zhu, Jinyu Li, Rui Tong, Xiaorong Zhang, Bo Yu

“…Autophagy was detected by immunofluorescence. RT-qPCR and western blot were carried out to determine the mRNA and protein expressions of Akt and mTOR. …”
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Effectiveness of Percutaneous Hypodermic Needle Release of Trigger Finger: A Prospective Study by R Sahaya Jose, M Syed Moosa, SL Binesh

“…Our aim of the study is to assess the pain and functional outcome following the percutaneous release of the trigger finger by using the visual analog scale (VAS), quick disabilities of the arm, shoulder, and hand (Q-DASH) score, and Quinnell's criteria, respectively, at regular follow-up intervals of 1, 3, 6 months, and 1 year. …”
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Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren’s Syndrome by Jiayao Fu, Yiping Pu, Baoli Wang, Hui Li, Xiujuan Yang, Lisong Xie, Huan Shi, Zhijun Wang, Junhao Yin, Tianle Zhan, Yanxiong Shao, Changyu Chen, Qi Luo, Jiabao Xu, Zirui Zong, Xindi Wei, Wanwen Xiao, Chuangqi Yu, Lingyan Zheng

“…In vivo, we injected different doses of BPTES into SS-like NOD/Ltj mice and found that 10 mg/kg BPTES significantly restored the salivary flow rate. Histological and qRT–PCR analyses showed that this concentration of BPTES attenuated lymphocytic infiltration and the numbers of PCNA-positive cells and CD4+ T cells. …”
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CircWDR78 inhibits the development of colorectal cancer by regulating the miR-653-3p/RGS4 axis by Chu Hao, Yunju Pu, Jiunian Li, Zhi Zhong, Zhaohui Huang, Xue Wang

“…Finally, the luciferase assay and qRT-PCR experiment proved that circWDR78 could sponge miR-653-3p, and confirmed that RGS4 is the downstream target of miR-653-3p. …”
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Computational Analysis of nsSNPs of ADA Gene in Severe Combined Immunodeficiency Using Molecular Modeling and Dynamics Simulation by Soukaina Essadssi, Al Mehdi Krami, Lamiae Elkhattabi, Zouhair Elkarhat, Ghita Amalou, Houria Abdelghaffar, Hassan Rouba, Abdelhamid Barakat

“…Using eleven prediction tools, we identified 15 nsSNPs (H15D, H15P, H17Q, H17Y, D19N, T26I, G140E, C153F, A183D, G216R, H258Y, C262Y, S291L, S291W, and K34OE) as harmful. …”
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Rapid and simple detection of Candida albicans using closed dumbbell-mediated isothermal amplification by Yanli Zhang, Xuhan Chen, Yeling Zhong, Fei Guo, Guifang Ouyang, Rui Mao

“…The limit of detection of Candida albicans DNA by CA-OL-CDA assay was 6.2×10-6 ng/μL of DNA (10 copies/μL), 10-fold more sensitive than real-time quantitative PCR (qPCR).DiscussionThe CA-OL-CDA assay exhibited advantages of high specificity, sensitivity, simpler and more efficient operation. …”
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Genetic Diversity of <i>Diaphorina citri</i> and Its Endosymbiont Across Diffusion Frontier and Epidemic Areas of Citrus Huanglongbing in China by Jin Yang, Ai-Jun Huang, Jun Zhou, Ping You, Xiang Li, Han Luo, Long Yi

“…We utilized mitochondrial genes (<i>COI</i>, <i>ND5</i>, and <i>Cytb</i>) of <i>D. citri</i> and housekeeping genes (<i>dnaQ</i>, <i>rpoC</i>, and <i>argH</i>) of its endosymbiont <i>Candidatus</i> Carsonella ruddii (<i>Ca.…”
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Opposing Effects of Omega-3 and Omega-6 Long Chain Polyunsaturated Fatty Acids on the Expression of Lipogenic Genes in Omental and Retroperitoneal Adipose Depots in the Rat by B. S. Muhlhausler, R. Cook-Johnson, M. James, D. Miljkovic, E. Duthoit, R. Gibson

“…Samples from distinct adipose depots (omental and retroperitoneal) were collected and mRNA expression of the pro-lipogenic transcription factors Sterol-Retinoid-Element-Binding-Protein1c (SREBP1c) and Peroxisome Proliferator Activated Receptor-γ (PPARγ), lipogenic enzymes Sterol-coenzyme Desaturase1 (SCD-1), Fatty Acid Synthase (FAS), lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (G3PDH) and adipokines leptin and adiponectin determined by qRT-PCR. Increasing dietary ALA content resulted in altered expression of SREBP1c, FAS and G3PDH mRNA in both adipose depots. …”
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Writers, readers, and erasers of N6-Methyladenosine (m6A) methylomes in oilseed rape: identification, molecular evolution, and expression profiling by Chaofan Shan, Kui Dong, Dongyu Wen, Ziyi Ye, Fei Hu, Meryem Zekraoui, Jun Cao

“…RNA-seq and quantitative real-time PCR (qRT-PCR) expression analyses revealed that the identified Bnam6As exhibit tissue-specific expression patterns, as well as their expression patterns in response to various abiotic and biotic stresses. …”
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The Application of Resolvin D1-Loaded Gelatin Methacrylate in a Rat Periodontitis Model by Zhe Xing, Jing Liu, Jiazheng Cai, Xiaofeng Jiang, Jingwen Liang, Masahito Fujio, Elin Hadler-Olsen, Jing Wang, Alpdogan Kantarci, Ying Xue

“…Periodontal inflammation and tissue regeneration were evaluated using quantitative PCR (qPCR) and histochemical staining, while alveolar bone repair and regeneration were analyzed through micro-CT. …”
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Understanding recruitment to a perioperative randomised controlled trial: protocol for a mixed-methods substudy nested within a feasibility trial of octreotide infusion during live... by Duncan Wagstaff, S Ramani Moonesinghe, Jez Fabes, Michael Spiro, Edgar Brodkin, Ee-Neng Loh, Vivienne Hannon

“…Recruitment consultations will be analysed using Q-QAT methodology and interviews will be analysed using framework analysis. …”
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Tetracycline and chloramphenicol exposure induce decreased susceptibility to tigecycline and genetic alterations in AcrAB-TolC efflux pump regulators in Escherichia coli and Klebsi... by Nian Anwar Nasralddin, Mehri Haeili, Sasan Karimzadeh, Fatemeh Alsahlani

“…The expression level of acrB was measured using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method. The excessive exposure (15 to 40 selection cycles) of studied bacteria to both antibiotics significantly decreased susceptibility of Tet-resistant (R) and Chl-R variants of E. coli (n = 6) and K. pneumoniae (n = 6) to several groups of antibiotics including tigecycline (4-16 and 8-64 times respectively) and quinolones. …”
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lncRNA NEAT1 Downregulation Ameliorates the Myocardial Infarction of Mice by Regulating the miR-582-5p/F2RL2 Axis by Zhenhua Wu, Yunpeng Bai, Yujuan Qi, Chao Chang, Yan Jiao, Yaobang Bai, Zhigang Guo

“…The expressions of F2RL2, miR-582-5p, and nuclear paraspeckle assembly transcript 1 (NEAT1) in myocardial tissues and HCMs were quantified by qRT-PCR or Western blot. Results. NEAT1 sponged miR-582-5p which targeted F2RL2. …”
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Appearance of L90I and N205S Mutations in Effector Domain of NS1 Gene of pdm (09) H1N1 Virus from India during 2009–2013 by Sachin Kumar, Shashi Khare, Bano Saidullah, Inderjeet Gandhoke, Hanu Ram, Supriya Singh, L. S. Chauhan, Arvind Rai

“…Mutations like K62R, K131Q, L147R, and A202P were observed for the first time in NS1 protein and their function remains to be determined.…”
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Identification and Verification of Hub Mitochondrial Dysfunction Genes in Osteoarthritis Based on Bioinformatics Analysis by Hui Niu, Xingxing Deng, Qian Zhang, Yijun Zhao, Jinfeng Wen, Wenyu Li, Huan Liu, Xiong Guo, Cuiyan Wu

“…The expression of hub genes in OA and normal chondrocytes was verified by external datasets, qRT-PCR and western blotting. Results. A total of 31 DEMDFGs were identified, with 15 genes upregulated and 16 genes downregulated. …”
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Multi Objective Prioritized Workflow Scheduling Using Deep Reinforcement Based Learning in Cloud Computing by Sudheer Mangalampalli, Syed Shakeel Hashmi, Amit Gupta, Ganesh Reddy Karri, K. Varada Rajkumar, Tulika Chakrabarti, Prasun Chakrabarti, Martin Margala

“…These priorities are fed to scheduler which uses Deep Q-Network model to dynamically schedule tasks by considering both priorities of tasks and VMs. …”
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