Long Noncoding RNA SNHG4 Attenuates the Injury of Myocardial Infarction via Regulating miR-148b-3p/DUSP1 Axis by Sheng Wang, Zhaoyun Cheng, Xianjie Chen, Guoqing Lu, Xiliang Zhu, Gaojun Xu

“…The expression of relating genes was measured by qRT-PCR and/or western blot. In vitro, cell viability was detected by MTT assay, and cell apoptosis was assessed by caspase-3 level, Bax/Bcl-2 expression, and/or flow cytometry. …”
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Efficacy of vaccines based on chimeric or multiepitope antigens for protection against visceral leishmaniasis: A systematic review. by Karine Ferreira Lopes, Mariana Lourenço Freire, Silvane Maria Fonseca Murta, Edward Oliveira

“…Limiting dilution was the most used method for assessing parasite load, followed by quantitative real-time polymerase chain reaction (qPCR). Most domains had an uncertain risk of bias due to insufficient information described.…”
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A Study Comparing Immunohistochemistry, Fluorescence in situ hybridization, GeneXpert® Breast Cancer STRAT4 Assay for Determining Hormone Receptor, Human Epidermal Growth Factor Re... by Rajaa El Aje, Abdellah Naya, Ayoub Khoaja, Younes Zaid, Mounia Oudghiri, Jodi Weidler, Mehdi Karkouri

“…The Xpert® Breast Cancer STRAT4, an RT-qPCR based system, can be used to classify invasive breast carcinomas based on the assessment of these four biomarkers; Methods: We evaluated ESR1, PGR, ERBB2, and MKi67 mRNA expression by Xpert Breast Cancer STRAT4 and ER, PR, HER2 and Ki67 by IHC (FISH for HER2 IHC 2+) in 200 formalin-fixed paraffin-embedded (FFPE) tissue blocks with invasive breast cancer, collected from the Pathology Department of Casablanca Ibn Rochd University Hospital; Results: Concordance between Xpert ® Breast Cancer STRAT4 and IHC was 93.5% for ER, 83.51% for PR, 95% for HER2 (92% for IHC+FISH), and 81.20% for Ki67 (excluding intermediate IHC Staining 10 ≤ %IHC <20). …”
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Elevation of Circulating miR-210 Participates in the Occurrence and Development of Type 2 Diabetes Mellitus and Its Complications by Xi Chen, Feng Tian, Zhilian Sun, Guoqing Zeng, Ping Tang

“…The miR-210 level in exosomes from serum was detected by qRT-PCR. Then, the correlations of BMI or miR-210 level with patients’ clinical characteristics were analyzed. …”
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The external validity of machine learning-based prediction scores from hematological parameters of COVID-19: A study using hospital records from Brazil, Italy, and Western Europe. by Ali Safdari, Chanda Sai Keshav, Deepanshu Mody, Kshitij Verma, Utsav Kaushal, Vaadeendra Kumar Burra, Sibnath Ray, Debashree Bandyopadhyay

“…The gold standard for COVID-19 detection, quantitative-Real-Time-Polymerase-Chain-Reaction (qRT-PCR), is expensive and time-consuming. Alternatively, haematology-based detections were fast and near-accurate, although those were less explored. …”
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Usp7 contributes to the tail regeneration of planarians via Islet/Wnt1 axis by Ang Liang, Jinglong Liu, Zhiyuan Zhang, Jing Xiao, Dezeng Liu, Zimei Dong, Guangwen Chen

“…Methods We identified an evolutionarily conserved gene, Usp7, and utilized RNA interference (RNAi), Quantitative real-time PCR (qRT-PCR), Whole-mount immunofluorescence, Tunnel, Whole-mount in situ hybridization (WISH), and western blotting to detect the function of Usp7 during the planarian regeneration. …”
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Thermal optimization and exergy efficiency of two new parabolic solar collectors using response surface methodology by Samaneh Baharloui, Mohammad Mohsen Peiravi, Mofid Gorji bandpy

“…The results illustrate the new design using circular collectors integrate with Nano particle increases the pressure drop but significantly improves the heat rate up to q=1075.6w/m2. Also, the performance of exergy efficiency improved for the collector mounted circular baffle by 30.45%. …”
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EZH2 Might Affect Macrophage Chemotaxis and Anti-Inflammatory Factors by Regulating CCL2 in Dental Pulp Inflammation by Ziqi Hu, Yingyi Chen, Jie He, He Liu, Tianqian Hui

“…The expressions of anti-inflammatory cytokine interleukins (IL-4 and IL-10) and transforming growth factor-β (TGF-β) in HDPCs which were treated by EZH2 complex, CCL2 complex, and CCL2 antibody were examined by quantitative real-time polymerase chain reaction (q-PCR). Results. The expression of TNF-α gradually increased in dental pulp inflammation. …”
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Banking service quality perceived by students: Implications to green services by Đokić Nenad, Milićević Nikola, Kalaš Branimir, Đokić Ines

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Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis by Jurate Savickiene, Grazina Treigyte, Sandra Baronaite, Giedre Valiuliene, Algirdas Kaupinis, Mindaugas Valius, Audrone Arlauskiene, Ruta Navakauskiene

“…AF-MSCs differentiated into adipocytes, osteocytes, chondrocytes, myocytes, and neuronal cells, as determined by morphological changes, cell staining, and RT-qPCR showing the tissue-specific gene presence for differentiated cell lineages. …”
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Seismic Fragility Analysis of Bridge Group Pile Foundations considering Fluid-Pile-Soil Interaction by Chao Zhang, Chengwang Wu, Piguang Wang

“…Based on the analysis platform OpenSees, the three-dimensional finite model of the bridge pile foundation is developed, where the pile-water interaction is replaced by the added mass method, nonlinear p-y, t-z, and q-z elements are used to simulate pile-soil interaction, and the displacement of the surface ground motion due to seismic excitations is applied on all spring supports. …”
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PPARγ activation attenuates neonatal CRD-induced visceral pain sensitization and anxiety in male rats by alleviating oxidative stress by Minjie Li, Xiyu Zhuo, Yongxiao Liu, Jinchao You, Jianqing Lin

“…Superoxide dismutase (SOD) and malondialdehyde (MDA) in the spinal cord were analyzed by water-soluble tetrazolium-1 (WST-1) and thiobarbituric acid (TBA) methods, respectively, the expression levels of PPARγ in the spinal cord were assessed by qRT-PCR and Western blotting. Results Neonatal CRD-induced rats showed visceral pain sensitization and anxiety in adulthood, with down-regulated expression of PPARγ and SOD and elevated MDA levels in the spinal cord. …”
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Meta-analysis of microarray and RNAseq data reveal OsbZIP52 to mediate salt stress responses in sensitive, tolerant and halophyte rice varieties by Dipankor Chatterjee, Mohammad Umer Sharif Shohan, Nishat Tamanna, Zeba I. Seraj

“…The expression level of OsbZIP52 was quantified using RT-qPCR and observed downregulated expression in salt stress in root and leaf tissues of four rice cultivars (2 salt-tolerant and 2 salt-sensitive). …”
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A Novel Rat Infant Model of Medial Temporal Lobe Epilepsy Reveals New Insight into the Molecular Biology and Epileptogenesis in the Developing Brain by Carola Wormuth, Anna Papazoglou, Christina Henseler, Dan Ehninger, Karl Broich, Britta Haenisch, Jürgen Hescheler, Rüdiger Köhling, Marco Weiergräber

“…We further analyzed selected candidate genes potentially relevant to epileptogenesis using a RT-qPCR approach. Several candidates, such as the low-voltage-activated Ca2+ channel Cav3.2 and the auxiliary subunits β1 and β2, which were previously reported to be upregulated in the hippocampus of the adult pilocarpine mTLE model, were found to be downregulated (together with Cav2.1, Cav2.3, M1, and M3) in the hippocampus and cortex of our InfRPil-mTLE model. …”
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LINC02389/miR-7-5p Regulated Cisplatin Resistance of Non-Small-Cell Lung Cancer via Promoting Oxidative Stress by Peng Ma, Wen Han, Cunying Meng, Xiaohong Tan, Pengfei Liu, Lei Dong

“…Materials and Methods. qRT-PCR and Western blot were performed to detect the expression of LINC02389 and miR-7-5p in NSCLC tissues and cell lines. …”
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Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38/MAPK Pathway by Xiuxia Wang, Yinmin Wang, Xianyu Zhou, Fei Liu

“…The transcript abundance of TGF-β1, as well as that of insulin-like growth factor binding protein 3 (IGF-BP3), was evaluated using qRT-PCR on Jurkat cells cultured in ADSC-CM for 24 hours. …”
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Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling Pathway by Ga Hee Jeong, Ki Chan Kim, Ji Hyun Lee

“…The results showed that CBG improved dermatitis severity score, epidermal thickness, and mast cell count and reduced inflammatory cytokines (<i>Tslp</i>, <i>Il1b</i>, <i>Il4</i>, <i>Il6</i>, <i>Il13</i>, <i>Il17</i>, <i>Il18</i>, <i>Il22</i>, and <i>Il33</i>) by qRT-PCR (<i>p</i> < 0.001). Western blot results showed modulated changes in JAK1, JAK2, TYK2, STAT1, STAT2, STAT3, p-STAT3, STAT6, and p-STAT6 (<i>p</i> < 0.05). …”
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ZNF169 promotes thyroid cancer progression via upregulating FBXW10 by Wen Luo, Qiyu Xiao, Ying Fu

“…Methods The expression of ZNF169 was measured using immunohistochemistry, RT-qPCR, and western blot. Cell proliferation was detected using CCK-8 assay and cell colony formation assays, while cell migration was determined by Transwell assay. …”
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Comparative gametogenesis and genomic signatures associated with pollen sterility in the seedless mutant of grapevine by Siddhi Chavan, Satish Phalake, Sujata Tetali, Vitthal T. Barvkar, Ravindra Patil

“…The RNAseq results were validated by qRT-PCR. Genome sequence data was also used to identify induced mutations in the seedless mutant, which revealed three homozygous and 25 heterozygous InDels in the genes related to male gametophyte development. …”
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Morphological and Molecular Characterization of Toxigenic Aspergillus flavus from Groundnut Kernels in Kenya by Robert O. Okayo, Darius O. Andika, Mathews M. Dida, George O. K’Otuto, Bernard M. Gichimu

“…The expression analysis of aflR, aflS, aflD, aflP, and aflQ genes in the aflatoxin biosynthesis pathways was conducted to confirm the positive identification of A. flavus. …”
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