Regulatory Role of rno-miR-30b-5p in IL-10 and Toll-like Receptor 4 Expressions of T Lymphocytes in Experimental Autoimmune Uveitis In Vitro by Yuanyuan Sun, Dadong Guo, Bin Liu, Xuewei Yin, Huixia Wei, Kai Tang, Hongsheng Bi

“…Meanwhile, the levels of rno-miR-30b-5p in eye tissues, spleen, and lymph nodes were measured using quantitative PCR (Q-PCR). IL-10 and TLR4 in spleen and lymph nodes were further separately determined by using Q-PCR and Enzyme-Linked Immunosorbent Assay (ELISA). …”
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The Influence of Cell Source and Donor Age on the Tenogenic Potential and Chemokine Secretion of Human Mesenchymal Stromal Cells by Weronika Zarychta-Wiśniewska, Anna Burdzińska, Katarzyna Zielniok, Marta Koblowska, Kamila Gala, Piotr Pędzisz, Roksana Iwanicka- Nowicka, Anna Fogtman, Aleksandra Aksamit, Agnieszka Kulesza, Aleksandra Zołocińska, Leszek Pączek

“…All these 10 factors were tested for gene expression by the qRT-PCR method, and all except of RUNX2 were additionally evaluated for protein expression or secretion. …”
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The impact of E × B shear on microtearing based transport in spherical tokamaks by B.S. Patel, M.R. Hardman, D. Kennedy, M. Giacomin, D. Dickinson, C.M. Roach

“…Fusion 65 045011) at low β when $q\sim 1$ , but the agreement worsens at higher q where the theory breaks down. …”
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Identification of robust and abundant reference transcripts for EV mRNA cargo normalization by Antje M. Zickler, Radosław Grochowski, André Görgens, Erik Bäcklin, Maximilian Kordes, J.-Matthias Löhr, Joel Z. Nordin, Samir EL Andaloussi, Daniel W. Hagey

“…As such, we subjected the RT-qPCR results from two independent oligo-dT cDNA synthesis methods to stability assessment using the RefFinder analysis tool, conducted a proof-of-concept normalization on the levels of the variably expressed gene RAB13 and compared quantification of engineered mRNA loading with that of digital PCR. …”
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A comprehensive, population level evaluation of previously reported drug triggers of pemphigus highlights immunomodulatory capacity as a common characteristic by Justin Baroukhian, Kristina Seiffert-Sinha, Animesh A. Sinha

“…Despite anecdotal reports linking multiple drugs to PV, corroborating evidence from large datasets is missing.ObjectiveTo examine the extent to which previously reported associations between a comprehensive list of 36 drugs implicated in PV pathogenesis could be replicated using population-level pharmacovigilance data.DesignSeries of observational, retrospective, case-control, pharmacovigilance analyses (one analysis/drug, 36 total).SettingPopulation based.ParticipantsIndividuals who submitted a report of a drug-related adverse event to the FDA from Q4 of 2003 to Q2 of 2023.ExposureCases were identified by the presence of adverse events described by the MedDRA preferred term “pemphigus” (10034280) and then sorted based on exposure to each of the drugs of interest.Main outcomes and measuresReporting Odds Ratios (RORs) quantifying the association between a given drug exposure and reports of pemphigus adverse events.ResultsThe analyses revealed statistically significant associations between reports of pemphigus and exposure to 11/36 previously reported drugs, two of which had particularly high RORs (>200) [gold sodium thiomalate (ROR, 266.0; 95% CI, 202.6-349.3) and hydroxychloroquine (ROR, 282.6; 95% CI, 261.0-306.1)], three had very strong RORs (14-45) [penicillamine (ROR, 30.5; 95% CI, 11.4-81.7), piroxicam (ROR, 14.8; 95% CI, 8.2-26.7), and imiquimod (ROR, 42.3; 95% CI, 26.2-68.3)], and six had modestly strong RORs (2-5) [rifampin (ROR, 2.8; 95% CI, 1.4-5.6), hydrochlorothiazide (ROR, 1.6; 95% CI, 1.2-2.1), irbesartan (ROR, 2.7; 95% CI, 1.6-4.4), lisinopril (ROR, 5.3; 95% CI, 4.5-6.2), nivolumab (ROR, 2.7; 95% CI, 1.8-4.1), and nifedipine (ROR, 3.0; 95% CI, 1.9-5.0)]. …”
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Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma by Yunxi Chen, Eva Jin, Mohamed Abdouh, Éric Bonneil, Daniel Alexander Jimenez Cruz, Thupten Tsering, Qianqian Zhou, Aurélie Fuentes-Rodriguez, Alexandra Bartolomucci, Alicia Goyeneche, Solange Landreville, Miguel N. Burnier, Julia V. Burnier

“…MutCMs were generated from 10 donors using GNAQ Q209L -carried lentivirus with high mutant copies (up to 98.8% of total GNAQ copies being mutant). …”
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LINC01089 governs the miR-1287-5p/HSPA4 axis to negatively regulate osteogenic differentiation of mesenchymal stem cells by Hao Zou, Fei Hu, Xin Wu, Bin Xu, Guifeng Shang, Dong An, Dehao Qin, Xiaolei Zhang, Aofei Yang

“…Methods: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) and western blotting were performed to analyze LINC01089, miR-1287-5p, and heat shock protein family A (HSP70) member 4 (HSPA4) expression. …”
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Promotion of microfracture-mediated cartilage repair by the intra-articular injection of Mg2+ by Zhian Chen, Tianhua Zhou, Zhengbo Yin, Peiya Duan, Ying Zhang, Yujiao Feng, Rongmao Shi, Yongqing Xu, Rongqing Pang, Hongbo Tan

“…The expression levels of related genes were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Results: The histological results showed that the 0.5 mol/L Mg2+ group had deeper positive staining in haematoxylin-eosin (H&E), safranin O, Alcian blue, and type II collagen staining. …”
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Genomic insights into familial adenomatous polyposis: unraveling a rare case with whole APC gene deletion and intellectual disability by Hiroki Tanabe, Masami Ijiri, Kenji Takahashi, Honoka Sasagawa, Tomomi Kamanaka, Shohei Kuroda, Hiroki Sato, Takeo Sarashina, Yusuke Mizukami, Yoshio Makita, Toshikatsu Okumura

“…Whole-genome array comparative genomic hybridization (aCGH) revealed germline deletions at chromosome 5q22.1-22.2 encompassing the entire APC gene. The patient and her son exhibited mild intellectual disability without developmental delay. …”
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Grid-Connected PV System for 324 kW with Improved Maximum PowerPoint Tracking by Abbas Hussein Khariy, Ahmed Rashid Ajel, Sadik Kamel Gharghan, Noaman Mohammad Noaman

“…The duty cycle is changed in an incremental conductance maximum power point tracking (MPPT) technique to reach the maximum power point (MMP). In a rotating d-q synchronous reference frame, the multilevel voltage source inverter converts the maximum voltage (VSI). power obtained from first stage power converter (DC-DC) to AC power utilizing a droop controller approach. …”
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Predicting Tunnel Squeezing Using Multiclass Support Vector Machines by Yang Sun, Xianda Feng, Lingqiang Yang

“…This study presents a modified application of a multiclass support vector machine (SVM) to predict tunnel squeezing based on four parameters, that is, diameter (D), buried depth (H), support stiffness (K), and rock tunneling quality index (Q). We compiled a database from the literature, including 117 case histories obtained from different countries such as India, Nepal, and Bhutan, to train the multiclass SVM model. …”
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Mayfly Taylor Optimisation-Based Scheduling Algorithm with Deep Reinforcement Learning for Dynamic Scheduling in Fog-Cloud Computing by G. Shruthi, Monica R. Mundada, B. J. Sowmya, S. Supreeth

“…The developed MTOA-based Deep Q-Network (DQN) showed better performance with energy consumption, service level agreement (SLA), and computation cost of 0.0162, 0.0114, and 0.0855, respectively.…”
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Diamond Blackfan Anemia at the Crossroad between Ribosome Biogenesis and Heme Metabolism by Deborah Chiabrando, Emanuela Tolosano

“…Interestingly, some DBA patients have disease linkage to chromosome 1q31, where FLVCR1 is mapped. Moreover, it has been reported that cells from DBA patients express alternatively spliced isoforms of FLVCR1 which encode non-functional proteins. …”
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Complexities of fonts in disfluent experiments by John Adjah, Lucy Afeafa Ry-Kottoh, Agnes Decardi-Nelson, Samiratu Abdulai Mamah, Eric Sam

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Identification of Geographical Origin of Milk by Amino Acid Profile Coupled with Chemometric Analysis by Min Kang, Quanhong Yue, Shikuan Jia, Jiarong Wang, Mengdan Zheng, Ran Suo

“…The predictive model for the geographical origin of milk samples was established by orthogonal partial least squares-discriminant analysis (OPLS-DA) with a classification accuracy of 100% and the performance parameters of R2X 0.98, R2Y 0.82, and Q2 0.75. The excellent predictive ability of the model was validated using the validation data set. …”
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Exploring the contemporary spatial and temporary dynamics of the settlement hierarchy and system in Serbia's Srem Region by Đerčan Bojan, Bjelajac Dajana, Bubalo-Živković Milka, Lukić Tamara, Gatarić Dragica, Pogrmić Zorica

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Exploration and construction of competency training model for non-orthopedics residents in orthopedics base by GU Honglin, CHEN Rong, YE Yongyu, DAI Guolin, LIANG Changxiang, CHANG Yunbing, LI Huiyan

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Síndrome de Cohen. Presentación de un caso by Elayne Esther Santana Hernández, Victor Jesús Tamayo Chang

“…Se ha localizado el locus para el síndrome de Cohen en el cromosoma 8q 22 (COH 1). Desde su descripción se reportan pocos casos, su diagnóstico es clínico a través de una delineación correcta del fenotipo. …”
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Analytical Solutions of Fractional Walter’s B Fluid with Applications by Qasem Al-Mdallal, Kashif Ali Abro, Ilyas Khan

“…The general solutions are expressed in the format of generalized Mittag-Leffler function MΩ2,Ω3Ω1χ and Fox-H function Hp,q+11,p satisfying imposed conditions on the problem. …”
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On stabilization of magnetically charged brane shell and over-extremality by Sohei Tsukahara

“…Moreover, we show the existence of over-extremal states such that (gQ)2 ≥ G 4 m 2 is satisfied in regions where the magnetic field is sufficiently large. …”
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