Preliminary investigation on the establishment of a new meibomian gland obstruction model and gene expression by Ming Sun, Huanmin Cheng, Zheng Yang, Jiangqin Tang, Shengshu Sun, Zhanglin Liu, Shaozhen Zhao, Lijie Dong, Yue Huang

“…Meanwhile, RNA sequencing and qPCR showed that lipid peroxidation (LPO), transient receptor potential vanilloid-3 (TRPV3) and genes in PPAR signaling pathway were differentially expressed in 16-week meibomian gland obstructive rats (p < 0.05). …”
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Robotic total knee arthroplasty safely reduces length of stay in an Asian public healthcare system by Kai C. A. Chan, Amy Cheung, Ping-Keung Chan, Michelle H. Luk, Kwong Y. Chiu, Henry Fu

“…Overall robotic use had risen to 20.4% (2023 Q3 to Q4: 355/1,738) by the end of 2023, with Mako being the most popular at 10.3% (179/1,738). …”
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miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 Pathway by Jiao Tian, Yiming He, Zihui Zhang, Yuxin Zhu, Haixia Ren, Liang Zhang, Lei Li, Wei Li, Weidong Zhang, Ting Xiao, Honggang Zhou, Xiaoping Li

“…Results The GEO database and qRT‐PCR analysis indicated that miR‐224‐5p was significantly downregulated in NSCLC patients and cell lines. …”
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The effects of Fraxini cortex and Andrographis herba on Escherichia coli-induced diarrhea in chicken by Yunying Wang, Zhenwei Sheng, Huicong Li, Xuewen Tan, Yingqiu Liu, Weimin Zhang, Wuren Ma, Lin Ma, Yunpeng Fan

“…Mass spectrometry technology and network pharmacology were used to analyze the optimal TCM formula corroborated by molecular docking and qPCR for further explore mechanism exploration. …”
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Comprehensive Analysis of m5C RNA Methylation Regulator Genes in Clear Cell Renal Cell Carcinoma by Jiajin Wu, Chao Hou, Yuhao Wang, Zhongyuan Wang, Pu Li, Zengjun Wang

“…Furthermore, we performed qRT-PCR experiments and measured global m5C RNA methylation level to validate this signature in vitro and tissue samples. …”
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Iron triggers TvPI4P5K proteostasis and Arf-mediated cell membrane trafficking to regulate PIP2 signaling crucial for multiple pathogenic activities of the parasitic protozoan Tric... by Kuan-Yi Wu, Yen-Ju Chen, Shu-Fan Lin, Hong-Ming Hsu

“…In dominant-active HA-TvArf220 Q71L mutant, TvPI4P5K plasma membrane trafficking, PIP2 production, and intracellular calcium levels were increased, while these processes were inhibited in dominant-negative T31N mutant or those by Brefeldin A (BFA) treatment. …”
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Genome-wide association study unravels mechanisms of brain glymphatic activity by Shu-Yi Huang, Yi-Jun Ge, Peng Ren, Bang-Sheng Wu, Weikang Gong, Jing Du, Shi-Dong Chen, Ju-Jiao Kang, Qing Ma, Arun L. W. Bokde, Sylvane Desrivières, Hugh Garavan, Antoine Grigis, Herve Lemaitre, Michael N. Smolka, Sarah Hohmann, IMAGEN Consortium, Jian-Feng Feng, Ya-Ru Zhang, Wei Cheng, Jin-Tai Yu

“…Genetic correlation and polygenic overlap analyses indicate a common underlying genetic mechanism between the ALPS-index, ventricular volumes, and cerebrospinal fluid tau levels, with GMNC (3q28) and C16orf95 (16q24.2) as the shared genetic basis. …”
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Identification and functional characterization of AsWRKY9, a WRKY transcription factor modulating alliin biosynthesis in garlic (Allium sativum L.) by Jiaying Wu, Min Li, Wanni Wang, Yiren Su, Jie Li, Jiaxin Gong, Xianfeng Meng, Chenyuan Lin, Qiantong Zhang, Yanyan Yang, Chunyan Xu, Limei Zeng, Jihong Jiang, Xuqin Yang

“…Further verification by electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation qPCR (ChIP-qPCR) confirmed that AsWRKY9 interacts by binding to the W-box site on the AsFMO1 promoter. …”
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Interactions between NAD+ metabolism and immune cell infiltration in ulcerative colitis: subtype identification and development of novel diagnostic models by Linglin Tian, Huiyang Gao, Tian Yao, Tian Yao, Yuhao Chen, Linna Gao, Jingxiang Han, Lanqi Zhu, He Huang, He Huang, He Huang

“…Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted to verify gene expression in cell lines.ResultsIn our study, UC patients were classified into two subtypes based on DE-NMRGs expression levels, with Cluster A exhibiting enhanced self-repair capabilities during inflammatory responses and Cluster B showing greater inflammation and tissue damage. …”
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First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024) by Paloma Kiwan, Eva Lopez, Morena Gasparine, Geraldine Piorkowski, Agathe Colmant, Achille Paguem, Stephanie Mvodo, Laurence Thirion, Xavier de Lamballerie, Remi Charrel, Alessandra Falchi

“…The quantitative reverse transcription polymerase chain reaction (qRT-PCR) screening of 251 tick pools yielded 61 JMTV-positive pools, of which 58 corresponded to R. …”
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The Association of Cell-Free LncH19 and miR-29b Expression with the PI3K/AKT/HIF-1/VEGF Pathway in Patients with Diabetic Nephropathy: In Silico Prediction and Clinical Validation by Noha M. Abd El-Fadeal, Basma Osman Sultan, Asmaa K. K. AbdelMaogood, Essam Al Ageeli, Fatma Tohamy Mekhamer, Sherihan Rohayem, Ahmed Shahidy, Nora Hosny, Manal S. Fawzy, Mohammed M. Ismail, Hidi A. A. Abdellatif

“…Furthermore, based on qPCR, the level of lncRNA H19 was negatively correlated with eGFR and miRNA-29b expression. …”
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Modeling Anomalous Transport of Cosmic Rays in the Heliosphere Using a Fractional Fokker–Planck Equation by José Luis Díaz Palencia

“…The model parameters, including the fractional orders <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>α</mi></semantics></math></inline-formula> and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>μ</mi></semantics></math></inline-formula> and the entropic index <i>q</i>, are estimated by a short comparison between theoretical predictions and observational data from cosmic ray experiments. …”
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Mass Transfer and Intrinsic Light Variability in the Contact Binary MT Cas by Haifeng Dai, Huiyu Yuan, Yuangui Yang

“…The results imply that MT Cas is a W-type weak-contact binary with a mass ratio of q=2.365(±0.005) and a fill-out factor of f=16.6(±1.2)%, respectively. …”
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Lymphoma of the Urinary Bladder by Anthony Kodzo-Grey Venyo

“…MALT lymphoma exhibits t(11; 18)(q21: 21). Radiotherapy is an effective treatment for the MALT type of LUB with no recurrence. …”
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Las juventudes rurales: tensiones entre los ciclos del trabajo rural y el doméstico. Estudio comparativo de casos en Argentina by Carla Daniela Rosales

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Technology, Electoral Malpractice and the Crisis of Election Administration in Nigeria by Daniel Godwin

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Four Central Nanocavity of Ultra-Sensitive Refractive Index Biosensor for Cancer Detection in Hexagonal Photonic Crystal by William Matsui, Jianqiao Fang

“…At best, the sensitivity and quality factor parameters are 734nm/RIU and Q.f=2330.09, respectively. The Figure of Merit (FOM) or detection limit is 412.87±45.87RIU-1. …”
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Prognostic Biomarker NUMB Is Inhibited by Breast Cancer Cell Exosomes to Promote Breast Cancer Progression by Xue Qin, Youde Cao

“…Meanwhile, Western blot and qPCR were conducted to determine the regulation of NUMB expression by exosomes in breast cancer cells. …”
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The importance of cash flow analysis by Ševkušić Ljubiša, Bodiroga Radomir

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