Clinical spectrum, treatment and outcomes of the m.10197G>A mutation in MT-ND3: a case report, systematic review and meta-analysis by YuZhi Shi, Bin Chen, SongTao Niu, XinGao Wang, ZaiQiang Zhang

“…The median age at onset for LS/LS+ was significantly younger than that for LHON/LHON+ [median, (Q1–Q3), 3.0 (0.58–9.5) vs. 13.5 (5.75–41.75), P = 0.001]. …”
Get full text

Optimizing Tongue Fluid Sampling and Testing Protocols for Enhanced PRRSV Isolation from Perinatal Swine Mortalities by Onyekachukwu Henry Osemeke, Isadora Machado, Elisa De Conti, Mariah Musskopf, Mafalda Pedro Mil-Homens, Samuel Stutzman, Baoqing Guo, Thomas Petznick, Gustavo De-Sousa-E Silva, Phillip Gauger, Jianqiang Zhang, Daniel C. L. Linhares

“…The mean RT-qPCR cycle threshold (Ct) values for the PBS, VTM, freeze-thaw, and homogenate groups were 21.9, 21.8, 22.6, and 24.8, respectively. …”
Get full text

Association between adult body shape index and serum levels of the anti-aging protein Klotho in adults: a population-based cross-sectional study of the NHANES from 2007 to 2016 by Li Gong, Jinghan Xu, Yiyang Zhuang, Liwei Zeng, Zhenfei Peng, Yuzhou Liu, Yinluan Huang, Yutian Chen, Fengyi Huang, Chunli Piao

“…When ABSI was divided into quartiles, after full adjustment, Klotho protein levels were lower in participants in the fourth fully adjusted ABSI quartile (Q4: -0.352 pg/ml) than in those in the lowest quartile (Q1) (P<0.0001).ConclusionThere was a negative linear correlation between ABSI score and serum Klotho concentration. …”
Get full text

Protein arginine methyltransferase 7 linked to schizophrenia through regulation of neural progenitor cell proliferation and differentiation by Ting Shen, Jing Yu, Bin Xie, Cuiping Huang, Jingjie Cui, Kefu Liu, Chunyu Liu, Chao Chen

“…Together, the results of this study reveal that PRMT7 is a functional gene at 16q22.1, contributing to the etiology of SCZ by modulating NPC proliferation and differentiation as an epigenetic regulator.…”
Get full text

Fermented sugarcane juice-derived probiotic Levilactobacillus brevis RAMULAB54 enhances lipid metabolism and glucose homeostasis through PPAR-γ activation by V. B. Chandana Kumari, Ramith Ramu, Sujay S. Huligere, Shashank M. Patil, Shivasharanappa Nayakvadi, Sharath Bijoor, Uma Venkateswaran Manjappara, Mohammad Z. Ahmed, Ling Shing Wong

“…LBR54 did not affect 3T3-L1 cell viability but reduced lipid accumulation by 13% and triglycerides by ≤ 44 mg/dL. qRT-PCR revealed upregulation of PPAR-γ and C/EBP-α, and downregulation of FAS, suggesting modulation of adipogenesis. …”
Get full text

AGD1/USP10/METTL13 complexes enhance cancer stem cells proliferation and diminish the therapeutic effect of docetaxel via CD44 m6A modification in castration resistant prostate can... by Hong Wang, Chunli Cui, Weiyi Li, Hui Wu, Jianjun Sha, Jiahua Pan, Wei Xue

“…Methods Quantitative real-time PCR (qPCR) was employed to determine the expression levels of AGD1 and METTL13 mRNAs in PCSCs and exosomes. …”
Get full text

Efficacy of biocompost from pineapple waste coupled with indigenous fungi strains Trichoderma spp. on soil fertility, nutrients uptake, growth, and yield of Ananas comosus (L.) M... by Nguyen Quoc Khuong, Truong Thi Kim Chung, Le Thi My Thu, Tran Chi Nhan, Lieu Mong Ca, Le Thanh Quang, Ly Ngoc Thanh Xuan, Ngo Thanh Phong

Get full text

بررسی رابطه واحدهای زمین‌شناسی با شدت فرسایش به کمک دو مدل با ساختار متفاوت (مطالعه موردی: حوزه آبخیز اخترآباد)... by بهارک معتمدوزیری, صادق واله, حسن احمدی

“…بررسی سازندها در مدل فارگاس نشان داده است که واحدهای سنگ چینه‌ای E1t1, E1t2, E2ig3, Q2f, Qt1 و Qt2 دارای شدت فرسایش بسیار شدید بوده‌اند؛ درحالی‌که در مدل پسیاک اصلاح شده واحدهای سنگ چینه‌ای E1t1 ,Qt1 و Qt2 دارای شدت فرسایش زیاد هستند که دلیل آن فرسایش­پذیری سازندها است، همچنین واحدهای سنگ چینه‌ای E1t2 و Q2t دارای شدت فرسایش خیلی زیاد هستند که دلیل آن علاوه بر فرسایش­پذیر بودن سازندها، تراکم بالای زهکشی در آن‌ها نیز هست.…”
Get full text

Extended-spectrum beta-lactamase and carbapenemase-producing Gram-negative bacteria in urinary tract infections in Ethiopia: a systematic review and meta-analysis by Mitkie Tigabie, Getnet Ayalew, Lidetu Demoze, Kebebe Tadesse, Yalewayker Gashaw, Muluneh Assefa

“…The presence of between-study heterogeneity was checked via the Cochrane Q statistic, and the magnitude was quantitatively measured via I2 statistics. …”
Get full text

Thermodynamics guided design and processing of a WC/HEA cermet tool for high temperature friction stir welding by Supreeth Gaddam, Surekha Yadav, Amit Kishan Behera, Noriaki Arai, Zaynab Mahbooba, Shikhar Krishn Jha, Qiaofu Zhang, Rajiv S. Mishra

Get full text

Soil phosphorus mobilization as affected by long-term organic fertilization in a tropical Regosol by Kalline De Almeida Alves Carneiro, Tancredo Augusto Feitosa De Souza, Gislaine Dos Santos Nascimento, Vânia Da Silva Fraga, Bruno de Oliveira Dias

Get full text

Multi-task aquatic toxicity prediction model based on multi-level features fusion by Xin Yang, Jianqiang Sun, Bingyu Jin, Yuer Lu, Jinyan Cheng, Jiaju Jiang, Qi Zhao, Jianwei Shuai

Get full text

Bone Defect Repair Using a Bone Substitute Supported by Mesenchymal Stem Cells Derived from the Umbilical Cord by Michal Kosinski, Anna Figiel-Dabrowska, Wioletta Lech, Lukasz Wieprzowski, Ryszard Strzalkowski, Damian Strzemecki, Lukasz Cheda, Jacek Lenart, Krystyna Domanska-Janik, Anna Sarnowska

“…WJ-MSCs in osteodifferentiation medium are able to attach and proliferate on Bio-Oss® scaffolds. Results obtained from qPCR and Luminex® indicate that WJ-MSCs possess the ability to differentiate into osteoblast-like cells and may induce osteoclastogenesis, angiogenesis, and mobilization of host MSCs. …”
Get full text

A dual-band microwave sensor for glucose measurements utilizing an enclosed split ring metamaterial-based array by Abhishek Kandwal, Ziheng Ju, Louis W.Y. Liu, Rohit Jasrotia, Choon Kit Chan, Zedong Nie, Ali M. Almuhlafi, Hamsakutty Vettikalladi

“…The backside of the PET substrate was fabricated with a defected ground plane designed to suppress the Q-factor associated with 4.5 GHz while leaving the Q-factor associated with 9.2 GHz unchanged. …”
Get full text

Droplets Cas13a‐RPA measurement delineates potential role for plasma circWDR37 in colorectal cancer by Jingsong Xu, Li Cao, Shuang Yang, Ying Jian, Yu Liu, Zhen Shen, Qian Liu, Xiang Chen, Min Li, Shun Li, Xiaolei Zuo, Min Li, Hua Wang

“…The arraystar circRNA microarray assays, functional experiments in vitro and in vivo, and qPCR were performed to discover and validate that circWDR37 is a biomarker for early screening and prognosis evaluation of CRC. …”
Get full text

Evaluation of Yellow Fever Virus Infection in <i>Aedes aegypti</i> Mosquitoes from Pakistan with Distinct Knockdown Resistance Genotypes by Carlucio Rocha dos Santos, Caleb Guedes Miranda dos Santos, Dinair Couto-Lima, Bárbara Silva Souza, Rafi Ur Rahman, Marcos Dornelas Ribeiro, José Bento Pereira Lima, Ademir Jesus Martins

“…After 14 days, RNA and DNA were extracted for viral RNA detection (qPCR) and <i>kdr</i> genotyping (TaqMan qPCR and HRM for T1520I and F1534C SNPs). …”
Get full text

3D-QSAR and Docking Studies of a Series of β-Carboline Derivatives as Antitumor Agents of PLK1 by Jahan B. Ghasemi, Valentin Davoudian

“…Validation of this model was performed using leave-one-out, q2loo of 0.85, and leave-multiple-out. This model gives a remarkably high r2pred(0.66) for a test set of 10 compounds. …”
Get full text

Performance features of virus removal filters with novel regenerated cellulose hollow fiber membranes by Tomoko Hongo-Hirasaki, Hiroki Fukutomi

“…Following the revision of International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) Q5A, the guidelines for the virus safety of biopharmaceuticals now state that an effective viral clearance process should achieve a viral reduction of 4 log10 or greater. …”
Get full text

Linear Methods for Analysis and Quality Control of Relative Expression Ratios from Quantitative Real-Time Polymerase Chain Reaction Experiments by Robert B. Page, Arnold J. Stromberg

“…Relative expression quantitative real-time polymerase chain reaction (RT-qPCR) experiments are a common means of estimating transcript abundances across biological groups and experimental treatments. …”
Get full text

A New Simple Method for the Strength of High-Strength Steel Thin-Walled Box Columns Subjected to Axial Force and Biaxial End Moments by Hong-Xia Shen

“…The strength of Q460 steel welded thin-walled box columns under biaxial bending is investigated by an FE model. …”
Get full text