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  1. 2501

    Transcutaneous vagus nerve stimulation as a pain modulator in knee osteoarthritis: a randomized controlled clinical trial by Gehad Gamal Elsehrawy, Maha Emad Ibrahim, Nermeen Hassan A.moneim, Mohamed Ahmed Hefny, Nashwa Kamel El Shaarawy

    Published 2025-01-01
    “…There were statistically significant improvements in the PD-Q and DN4 scores in the active tVNS group (P < 0.001), whereas in the sham group, DN4 questionnaire did not show any improvement. …”
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  2. 2502
  3. 2503

    Intraocular lens calculation formula selection for short eyes: based on axial length and anterior chamber depth by Ruoxi Gao, Jiaqing Zhang, Xiaotong Han, Yiguo Huang, Ruoxuan Huang, Jinfeng Ye, Ling Wen, Xiaozhang Qiu, Xiaoyun Chen, Xuhua Tan, Lixia Luo

    Published 2025-01-01
    “…The BUII, Hoffer QST, Hoffer Q, Holladay 1, and SRK/T showed myopic shifts (-0.49 to -0.18 diopters [D], P < 0.05), whereas K6 displayed a hyperopic shift (0.11 D, P = 0.03). …”
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  4. 2504

    Decreased Spp1 Expression in Acute Myocardial Infarction after Ischemia and Reperfusion Injury by Ling Li, Jungang Huang, Zongkai Zhao, Zhuzhi Wen, Kang Li, Tianjiao Ma, Lisui Zhang, Junmeng Zheng, Shi Liang

    Published 2021-01-01
    “…In the end, we used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting to measure and compare the expression of Spp1 in patients who accepted percutaneous coronary intervention. …”
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  5. 2505

    Unlocking melanoma Suppression: Insights from Plasma-Induced potent miRNAs through PI3K-AKT-ZEB1 axis by Pradeep Bhartiya, Apurva Jaiswal, Manorma Negi, Neha Kaushik, Eun Ha Choi, Nagendra Kumar Kaushik

    Published 2025-02-01
    “…Persistent NTP exposure for 5 min increased differential miRNA regulation, PI uptake, and the expression of genes involved in cell cycle arrest and death. qPCR confirmed that miR-200b-3p and miR-215-5p upregulation contributed to decreased cell viability and migration. …”
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  6. 2506

    Inhibition of id-1 reduces osteosarcoma growth and metastasis through mediation of snail by Rongbing Shu, Zhuanyi Yu, Jianmin Wu, Qiuxin Cheng, Zhihao Peng, Huaqiang Zhou, Min Zhao

    Published 2025-01-01
    “…The expression of Id-1 and Snail was evaluated by RT-qPCR and Western blotting.Cells were randomly divided into the Control group, sh-NC group, and sh-Id-1 group using lentiviral infection. …”
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  7. 2507
  8. 2508

    Disease control and quality of life in chronic spontaneous urticaria and recurrent angioedema are strongly linked, but not in all patients by Sophia Neisinger, Pascale Salameh, Annika Gutsche, Felix Aulenbacher, Frank Siebenhaar, Marcus Maurer

    Published 2025-01-01
    “…In CSU, QoL was impaired in 69.2% of patients based on the CU‐Q2oL and in 62.7% of patients based on a single numeric question from the UCT, with a mismatch in 89/445 patients. …”
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  9. 2509

    MiR-155-Mediated Deregulation of GPER1 Plays an Important Role in the Gender Differences Related to Inflammatory Bowel Disease by Xiaojuan Shao, Jintao Li, Fumin Xu, Dongfeng Chen, Kaijun Liu

    Published 2020-01-01
    “…The serum cytokine levels in the patients with IBD and the health donors were detected by ELISA, and the estrogen receptor level measurements for all of the participants were assessed by immunohistochemistry (IHC) and quantitative real-time PCR (qPCR). The miR-155 levels were detected by qPCR in all of the participants, and miR-155−/− mice were used to investigate the mechanism of miR-155 in the pathogenesis of IBD. …”
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  10. 2510

    Multidimension Analysis of the Prognostic Value, Immune Regulatory Function, and ceRNA Network of LY6E in Individuals with Colorectal Cancer by Ting Li, Weidong Liu, Chun Wang, Man Wang, Wenjia Hui, Jiajie Lu, Feng Gao

    Published 2022-01-01
    “…A ceRNA expression-based nomogram was established. Real-time PCR (qRT-PCR) was applied to validate the expression of LY6E-related ceRNA in CRC cell lines. …”
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  11. 2511

    Joint association of sleep patterns and oxidative balance score with all-cause and cardiovascular mortality among the general population by Chen Chen, Hanzhang Wu, Hanzhang Wu, Hongyu Jin, Zhiping Jiang, Wei Wang, Xiao Tan, Xiao Tan, Wen-Yang Li

    Published 2025-01-01
    “…Cox proportional hazards model was conducted to evaluate the association between sleep patterns or OBS alone and combined with all-cause and CVD mortality.ResultsPoor sleep patterns and pro-oxidant OBS (Q1 &amp; Q2) were identified as risk factors for mortality. …”
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  12. 2512

    CircCCT2/miR-146a-5p/IRAK1 axis promotes the development of head and neck squamous cell carcinoma by Long He, Lanruo Li, Liting Zhao, Xiaoya Guan, Yujia Guo, Qi Han, Huina Guo, Hongliang Liu, Chunming Zhang

    Published 2025-01-01
    “…Methods CircCCT2 expression levels in HNSCC tissues and cells were measured via qPCR. CircCCT2 was characterized by Sanger sequencing, qRT-PCR, RNase R & Actinomycin D treatment, nucleoplasmic separation and FISH experiments. …”
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  13. 2513

    Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments by Qiyao Liu, Tingyu Zhang, Yongan Ye, Xin Sun, Huan Xia, Xu Cao, Xiaoke Li, Wenying Qi, Yue Chen, Xiaobin Zao

    Published 2025-01-01
    “…Serum and liver tissues of the mice were collected for the detection of indicators, pathological staining, and expression analysis of hub targets using quantitative real-time polymerase chain reaction (qRT-PCR). Results: We identified 368 overlapping genes (OLGs) between SXN and LC targets. …”
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  14. 2514

    Carbon sequestration effects of the typical restored vegetation types in Eastern Qinling Mountains by XU Xiaoming, YAN Shanshan, PENG Xiaobang

    Published 2025-01-01
    “…Franco (16 325.2 g/m2) > Q. variabilis Blume (16 073.0 g/m2) > P . tabuliformis-Q. variabilis mixed forests (11 729.5 g/m2) > R. pseudoacacia L. (9 023.9 g/m2) > P. tabuliformis Carr. (8 610.1 g/m2) > grassland (7 177.9 g/m2) > farmland (5 496.2 g/m2). …”
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  15. 2515
  16. 2516

    Identification and validation of diagnostic genes IFI44 and IRF9 in insomnia-associated autoimmune uveitis by Chao Wu, Hui Feng, Meng Tian, Baorui Chu, Xianyang Liu, Shuhao Zeng, Yakun Wang, Hong Wang, Shengping Hou, Qingfeng Liang

    Published 2025-01-01
    “…Intersection of the above-mentioned key genes and compounds identified six genes and five compounds. After verification by qPCR and ROC curve analysis, IFI44 and IRF9 were confirmed as hub genes. …”
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  17. 2517

    Transcriptome Analysis of Effect of Cordyceps militaris Aqueous Extract in Human Gastric Cancer Cell BGC-823 on Different RCD Pathways by Mingqi CUI, Kaiwen YU, Yang SONG, Fangxu XU, Shenghou WANG, Ze WANG

    Published 2025-02-01
    “…Laser confocal microscopy, flow cytometry, and quantitative real time polymerase chain reaction (qRT-PCR) were employed to further validate the induction of apoptosis in gastric cancer cells by the XG aqueous extract. …”
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  18. 2518

    Distinct Murine Pancreatic Transcriptomic Signatures during Chronic Pancreatitis Recovery by Yinjie Zhang, Baibing Yang, Joy M. Davis, Madeline M. Drake, Mamoun Younes, Qiang Shen, Zhongming Zhao, Yanna Cao, Tien C. Ko

    Published 2021-01-01
    “…Pancreatic RNAs were extracted for RNA-seq and quantitative (q) PCR validation. Using RNA-seq, we identified a total of 3,600 differentially expressed genes (DEGs) in CP versus CON and 166 DEGs in CPR versus CONR. …”
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  19. 2519

    PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway by Xu Xu, Yuan-Yue Tang, Xiaohong Liang, Wen Luo, Dong-Mei Jiang, Jie Chen

    Published 2025-02-01
    “…PTEN and related protein levels were detected using RT-qPCR and Western blot. Statistical analyses were performed using the Mann-Whitney U test and Kruskal-Wallis H test. …”
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  20. 2520

    Dataset on electricity usage measurement for lower-to-middle-income primary and secondary schools in Western Cape, South AfricaMendeley Data by Jason Avron Samuels, Terhemba Michael-Ahile, M. J. (Thinus) Booysen

    Published 2025-04-01
    “…Public primary and secondary schools in South Africa are categorized into quintiles from Q1 (least economically advantaged) to Q5 (most affluent), a system designed to address economic disparities by considering the economic status of the surrounding community and the existing school infrastructure. …”
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