Prediction of Poor Outcome Using the Urea to Albumin Ratio in Thoracic Empyema by Evgeni Dimitrov, Daniel Valchev, Georgi Minkov, Emil Enchev, Yovcho Yovtchev

“…The UAR showed the best ability to prognosticate mortality compared to qSOFA (AUROC = 0.828 vs 0.747) and SIRS (AUROC = 0.828 vs 0.676). …”
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Association of oxidative balance score with all-cause and cardiovascular mortality in overweight and obese by Shuxin Ying, Hongyan Ding, Yanjin Chen, Su Zheng

“…According to Kaplan–Meier analysis, mortality was highest among participants in the lowest OBS quartile (Q1) and lowest among those in the highest quartile (Q4). …”
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Characterization of novel human endogenous retrovirus structures on chromosomes 6 and 7 by Nicholas Pasternack, Nicholas Pasternack, Ole Paulsen, Avindra Nath

“…These results demonstrate a clear, previously unreported, relationship between HML-2 loci 6q14.1 and 7p22.1a and highlight the utility of long-read sequencing to study repetitive elements. …”
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Liver macrophage-derived exosomal miRNA-342-3p promotes liver fibrosis by inhibiting HPCAL1 in stellate cells by Wenshuai Li, Lirong Chen, Qi Zhou, Tiansheng Huang, Wanwei Zheng, Feifei Luo, Zhong Guang Luo, Jun Zhang, Jie Liu

“…The functions of miR-342-3p in exosomes were verified by qPCR and luciferase reporter gene experiments with HSCs. …”
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Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry by Junmei Ma, Qiang Li, Sufang Fan, Liangna He, Lei Sun, Di Wen, Hongwen Zhai, Yan Zhang

“…The analytes were determined by Q-TOF/MS in TOF MS and information dependent acquisition (IDA)-MS/MS mode. …”
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Efficiency of Automated Viral RNA Purification for Pediatric Studies of Dengue and Zika in Hyperendemic Areas by Sandra L. Delgado, Piedad M. Perilla, Doris M. Salgado, María Clemencia Rojas, Carlos F. Narváez

“…For the evaluation of ZIKV RNA, the commercial TaqMan Triplex® assay was used, along with a well-tested in-house RT-qPCR assay. The concentration of RNA (5.2 vs. 7.5 ng/μL, P=0.03) and the number of integral bands (9 vs. 11) were higher with the high-throughput protocol. …”
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Dysregulation of c-Jun (JUN) and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) in obese people and their predictive values for metabolic syndrome by Chenxi Yang, Yi Chen, Guangfeng Tang, Tongtong Shen, Li Li

“…The expression profiles and RT-qPCR results showed that JUN and FOSB were highly expressed in individuals with obesity, obese mouse models, and patients with MetS. …”
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Association between serum albumin levels and erectile dysfunction in American Adults: A cross-sectional study from NHANES 2001-2004. by Haibin Wen, Zhenyu Lan, Xueming Liang, Huabin Su, Yuqi Qin

“…Higher ALB quartiles were significantly correlated with a decreased risk of ED [Q4 vs. Q1: OR: 0.56 (0.35-0.90), P =  0.02; P for trend =  0.03]. …”
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Effect of Black Cumin on the Expression of Genes Related to Lipid Metabolism in Adipose Tissue: A Mouse Model Study by Roya Maleki, Ali Ahadi, Hoda Ayat

“…The expression changes of Leptin, AdipoQ, and PPARG genes were measured by real-time RT-PCR. …”
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Identification and validation of hub gene TNFAIP6 of fibroblast-like synoviocytes in rheumatoid arthritis by LEI Lei, LYU Yuxin, ZHANG Jing

“…The expression of TNFAIP6 in human RA fibroblast-like synovial cell line MH7A was detected by RT-qPCR and Western blotting under simulated inflammatory environment. …”
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Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics by Lili Xu, Yang Jiao, Weiliang Cui, Bing Wang, Dongxiao Guo, Fei Xue, Xiangrong Mu, Huifen Li, Yongqiang Lin, Huibin Lin

“…We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, the quantitative methods based on HPLC-DAD and LC-MS/MS were connected to the synchronous quantitative assurance of eleven and fourteen marker compounds. …”
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Knockdown of Adra2a Increases Secretion of Growth Factors and Wound Healing Ability in Diabetic Adipose-Derived Stem Cells by Xiangyuan Zhao, Yong Zhang, Xinzhen Zuo, Shubai Wang, Xiao Dong

“…The differential genes were verified by real-time RT-qPCR. Clonidine was used to active Adra2a, and lentivirus-mediated RNAi was used to knockdown Adra2a. …”
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Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice by Liang Dong, Lang Li

“…Acute lung inflammation was measured by the cytokines in the lung homogenate and BALF using RT-qPCR and ELISA, respectively. Lung injury was evaluated by HE staining and lung injury scoring. …”
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Optimization of Butanol Production from Mixed Sugars and Sweet Sorghum Bagasse Hydrolysate Using <i>Clostridium beijerinckii</i> TISTR 1461 by Chalida Daengbussadee, Lakkana Laopaiboon, Thanawat Thanapornsin, Pattana Laopaiboon

“…The highest butanol concentration (<i>P_B</i>, 10.25–10.60 g/L), butanol yield (<i>Y_B/S</i>, 0.27–0.28 g/g), butanol productivity (<i>Q_B</i>, 0.22 g/L·h), and sugar consumption (<i>SC</i>, 61–63%) were achieved when the glucose content was at least 75% of the total sugars. …”
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Translational regulation of PKD1 by evolutionarily conserved upstream open reading frames by Lei Chen, Xia Gao, Xiangshen Liu, Ye Zhu, Dong Wang

“…Luciferase reporter assays and RT-qPCR results reveal that rs2092942382 and rs1596636969 increase, while rs2092942900 decreases main gene translation without affecting transcription. …”
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Navigating uncertainty in environmental DNA detection of a nuisance marine macroalga. by Patrick K Nichols, Kaʻuaʻoa M S Fraiola, Alison R Sherwood, Brian B Hauk, Keolohilani H Lopes, Colt A Davis, James T Fumo, Chelsie W W Counsell, Taylor M Williams, Heather L Spalding, Peter B Marko

“…We verified the eDNA assay using site-occupancy detection modeling from quantitative polymerase chain reaction (qPCR) data, calibrated with visual estimates of benthic cover of C. tumulosa that ranged from < 1% to 95%. …”
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Numerical Approximations and Fractional Calculus: Extending Boole’s Rule with Riemann–LiouvilleFractional Integral Inequalities by Abdul Mateen, Wali Haider, Asia Shehzadi, Hüseyin Budak, Bandar Bin-Mohsin

“…Future directions for this research include extending the framework to incorporate <i>q</i>-calculus, symmetrized <i>q</i>-calculus, alternative fractional operators, multiplicative calculus, and multidimensional spaces. …”
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Computational and In silico study of novel fungicides against combating root rot, gray mold, fusarium wilt, and cereal rust. by Mollah Naimuzzaman, Md Mahabub Hasan, Ajoy Kumer, Abu Yousuf Hossin, Mohammad Harun-Ur-Rashid, Swapan Kumar Roy, Abu Noman Faruq Ahmmed, Jamal Uddin

“…Next, two docking tools, such as AutoDock by PyRx and Molecular Docking by Glide from the Schrödinger suite, were used to convey the docking score, and ligand protein interactions against four main proteases, for instance 7VEM, 8H6Q, 8EBB, and 7XDS having name of pathogens: Phytophthora capsici, Botrytis cinerea, Fusarium oxysporum f. sp. lycopersici, and Puccinia graminis f. sp. tritici, respectively. …”
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UHPLC-UV method validation for simultaneous quantification of vitexin and isovitexin from Santalum album L. leaves by Thi Kim Phung Phan, Minh Quan Le, Kien Duc Ngo

“…The quantification procedure was performed on a Hypersil GOLD aQ Column (3 μm; 150 × 2.1 mm) at 35°C, with a mobile phase of acetonitrile (A) and and water with 0.1% formic acid (B), a flow rate of 0.3 mL/min, a detection wavelength of 336 nm, and an injection volume of 3 µL. …”
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Multispectral Polarization State Analyzer of Scanning Polarimeter ScanPol by Ivan Syniavskyi, Yevgen Oberemok, Yuriy Ivanov, Mikhail Sosonkin

“…Stokes parameters I, Q, and U are used to determine a degree of linear polarization of radiation that will allow obtaining the phase function and polarization characteristics of aerosol particle scattering, estimate their size, and determine the aerosol type and optical thickness. …”
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