circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis by Yandong Huang, Qinyang Bai, Zhanlong Wang, Hongbo Yu, Yanru Li, Hao Lu, Huimin Kang, Xuewei Shi, Kai Feng

“…First, the circ_0052184 expression in clinical tissue and cell samples was analyzed by qRT-PCR. Then, we constructed circ_0052184-silenced CRC cells and detected by qRT-PCR. …”
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Transcriptome analysis reveals the molecular mechanism of γ-linolenic acid eradicating the biofilm of vancomycin-resistant Enterococcus faecium by Ming Wei, Peng Wang, Tianmeng Li, Jun Liu, Yu Wang, Li Gu, Shuai Wang

“…Validation by qRT-PCR in six VRE-fm isolates confirmed the significant changes in the expression levels of all genes except for lpxtg-cwa, with statistical significance. …”
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Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines by Stefano Guariniello, Giovanni Di Bernardo, Giovanni Colonna, Marcella Cammarota, Giuseppe Castello, Susan Costantini

“…In the present paper we have carried out a global analysis of the selenotranscriptome expression in HepG2 and Huh7 cells compared to the normal human hepatocytes by reverse transcription-qPCR (RT-qPCR). Our data showed that in both cells there are three downregulated (DIO1, DIO2, and SELO) and ten upregulated (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1) genes. …”
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Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress by WU Tian-yi, WU Yi, FANG Zhi-yi, ZHANG Xiao-pei, WANG Fu-ling, XUE Ding-rong

“…The results demonstrated consistency between RT-qPCR and transcriptome sequencing analysis. This study offers preliminary insights into the molecular mechanisms of C. ferrugineus adaptation to low-temperature stress, forming a foundation for applying low-temperature grain storage technology in integrated pest management strategies.…”
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Transformation induced plasticity (TRIP) due to precipitation during continuous tempering of quenched and tempered steels by Niki Nouri, Elena Hillenmeyer, Thanusan Thavarajan, Stefan Dietrich, Volker Schulze

“…Quenched and tempered (Q&T) steels are often tempered to adjust the strength-ductility ratio by taking advantage of the precipitation of carbides. …”
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Evolution of SARS-CoV-2 in white-tailed deer in Pennsylvania 2021-2024. by Andrew D Marques, Matthew Hogenauer, Natalie Bauer, Michelle Gibison, Beatrice DeMarco, Scott Sherrill-Mix, Carter Merenstein, Ronald G Collman, Roderick B Gagne, Frederic D Bushman

“…To assess viral load and genotypes, RNA was isolated from retropharyngeal lymph nodes and analyzed using RT-qPCR and viral whole genome sequencing. Samples showed a 14.64% positivity rate by RT-qPCR. …”
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Aligning Indonesia's economic goals with SDGS: Strengthening Qur'anic principles in Islamic finance by Azwar Azwar, Abur Hamdi Usman

“…Methodology – Quarterly time-series data from Q1 2014 to Q1 2022 were analyzed using the cointegration and ARDL approaches. …”
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Comparative Analyses of Antiviral Potencies of Second-Generation Integrase Strand Transfer Inhibitors (INSTIs) and the Developmental Compound 4d Against a Panel of Integrase Quadru... by Steven J. Smith, Xue Zhi Zhao, Stephen H. Hughes, Terrence R. Burke

“…The mutations are centered around G140S/Q148H, including positions L74, E92, and T97 combined with E138A/K/G140S/Q148H. …”
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Sleep Deprivation Influences Circadian Gene Expression in the Lateral Habenula by Beilin Zhang, Yanxia Gao, Yang Li, Jing Yang, Hua Zhao

“…LHb-lesioning decreased the amplitude of reduced wake time and increased NREMS following sleep deprivation in rats. qPCR results demonstrated that Per2 expression was elevated after sleep deprivation, while the other two genes were unaffected. …”
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Endogenous economic growth from the perspective of Islamic economic doctrine by Qasemi Abdulkhaliq, Tavakkoli Mohammad Javad

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A QSAR Study Based on SVM for the Compound of Hydroxyl Benzoic Esters by Li Wen, Qing Li, Wei Li, Qiao Cai, Yong-Ming Cai

“…The results show that R2 and Q2LOO of 4 kinds of nonlinear models are more than 0.6 and SDEPext is 0.213, 0.222, 0.189, and 0.218, respectively. …”
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Algunas consideraciones para el cálculo del tamaño muestral en investigaciones de las Ciencias Médicas by Manuel E. Cortés Cortés, Norma Mur Villar, Miriam Iglesias León, Manuel Cortés Iglesias

“…Se expone la fórmula del muestreo para media y varianza conocidas, se da una técnica para encontrar el número de elementos de la muestra inicial cuando no se tienen conocimientos previos de media y varianza; se estudia el muestreo aleatorio simple de proporciones con las probabilidades de éxito y fracaso p y q respectivamente, explicándose el caso cuando se tiene p = q = 0.5 para la obtención de un tamaño de la muestra máximo.…”
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Experimental Study of the Effect of the Expansion Segment Geometry on the Atomization of a Plain-Jet Airblast Atomizer by Yi Jin, Kanghong Yao, Xiaomin He, Kai Zhang, Yunbiao Wang

“…The results show that more uniform droplet size distribution can be achieved with the addition of expansion segment, and the droplet size distribution factor q of the case adding the expansion segment is 52.8% bigger than that of the case with no expansion segment. q increases as the length and angle of expansion segment increase. …”
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Rapid detection of FAdV-4 by one-tube RPA-CRISPR/Cas12a assay by Lei Ma, Xueping Wang, Mingliang Zhang, Mengjie Zhu

“…Additionally, the agreement rate between the results of RPA-CRISPR/Cas12a and qPCR for detecting clinical samples is as high as 97.5%.DiscussionTherefore, the RPA-CRISPR/Cas12a assay presents a promising alternative for the simple, sensitive, and specific identification of FAdV-4.…”
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RNA Sequencing Reveals the Differentially Expressed circRNAs between Stable and Unstable Carotid Atherosclerotic Plaques by Xueguang Lin, Ying Deng, Lujuan Ye, Bo Chen, Jindong Tong, Weijun Shi, Bo Wang, Bo Yu, Jingdong Tang

“…DE circRNAs were screened, and six DE circRNAs were verified using quantitative real-time PCR (qRT-PCR). Functional evaluation of the DE circRNAs was conducted via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. …”
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Population Pharmacokinetics of Vancomycin in Thai Patients by Tunggul Adi Purwonugroho, Suvatna Chulavatnatol, Yupaporn Preechagoon, Busba Chindavijak, Kumthorn Malathum, Pakwan Bunuparadah

“…The interindividual variability for CL, 𝑉1, 𝑄, and 𝑉2 was 35.78, 20.93, 39.50, and 57.27%, respectively. …”
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Relationship between METS-IR and ABSI index and the prevalence of nocturia: a cross-sectional analysis from the 2005–2020 NHANES data by Fei Xue, Yating Zhou

“…Specifically, compared to the lowest quartile (Q1), participants in the highest quartile (Q4) had an OR of 1.45 (95% CI: 1.30–1.61, p < 0.0001) for METS-IR and 1.38 (95% CI: 1.23–1.55, p < 0.0001) for ABSI. …”
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Optimization of Bulk Cargo Terminal Unloading and Outbound Operations Based on a Deep Reinforcement Learning Framework by Haijiang Li, Jiapeng Zhao, Peng Jia, Hongdong Ou, Weili Zhao

“…Secondly, this study proposes an advanced solution methodology that employs a reinforcement learning technique integrating a Dueling Deep Q-Network and Double Deep Q-Network. This hybrid algorithm significantly enhances optimization performance and accelerates the learning process, thereby improving the efficiency of the solutions. …”
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Variability of mitochondrial DNA D-loop sequences in Zabaikalskaya horse breed by L. A. Khrabrova, N. V. Blohina, B. Z. Bazaron, T. N. Khamiruev

“…The haplogroups Q (25.81 %), B (19.35 %), G (16.13 %) and H (12.90 %) were prevailing in the mtDNA structure of this breed. …”
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STUDY OF TRANSIENT AND STATIONARY OPERATION MODES OF SYNCHRONOUS SYSTEM CONSISTING IN TWO MACHINES by V. S. Safaryan

“…In the article mathematical models of transient process of a two-machine system in natural form and in d–q coordinate system are presented. The mathematical model of two-machine system is considered in case of two windings of excitement at the rotors. …”
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