MT1G promotes iron autophagy and inhibits the function of gastric cancer cell lines by intervening in GPX4/SQSTM1 by Kaiqiang Meng, Jian Song, Fan Qi, Jiamin Li, Zhichao Fang, Liang Song

“…Perform bioinformatics analysis using the TCGA database to investigate the expression of MT1G in GC. RT-qPCR and Western blot were used to detect the expression of MT1G, ferroptosis related proteins, autophagy related proteins and ARNTL clock autophagy related proteins in Hgc27, MKN45 and AGS cell lines. …”
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Identification of Anoikis-Related Genes in Chronic Kidney Disease Based on Bioinformatics Analysis Combined with Experimental Validation by Liu H, Mei M, Zhong H, Lin S, Luo J, Huang S, Zhou J

“…And then, we adopted the quantitative real-time PCR (RT-qPCR) assay to validate the hub genes among several CKD animal models. …”
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Patient-Reported Outcomes in Children Undergoing the Modified Green Procedure for Treating Sprengel’s Deformity: Results from a Multicentric Study by Giovanni Trisolino, Paola Zarantonello, Marco Todisco, Giovanni Luigi Di Gennaro, Grazia Chiara Menozzi, Philipp Scheider, Alessandro Depaoli, Diego Antonioli, Gino Rocca, Sebastian Farr

“…At follow-up, 67.5% of patients had a qDASH score < 7, highlighting excellent functional outcomes. …”
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Enrichment of electrotrophic microorganisms from contrasting shallow-sea hydrothermal environments in bioelectrochemical reactors by Antoine Carissimo, Victoria Comes, Alenica Heussner, Anne-Hélène Prime, Roy E. Price, Gaël Erauso, Pierre-Pol Liebgott, Sven Kerzenmacher, Guillaume Pillot

“…A Multi-Factor Analysis revealed that the overall bioelectrochemical performances of these reactors set them apart from all the other Panarea and Prony conditions, not only due to their higher current production but also archaeal abundances (measured through qPCR). Most reactors produced organic acids (up to 2.9 mM in 6 days). …”
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Establishment of a prognostic signature and immune infiltration characteristics for uterine corpus endometrial carcinoma based on a disulfidptosis/ferroptosis-associated signature by Yong Huang, Huibin Li, Zhifu Wei, Wanshan He, Bin Chen, Shuang Cheng, Zhifang Zhao, Lv Deng, Lv Deng, Xiaohua Chen, Yu Lin, Yu Lin, Xiaoshan Hong, Xiaoshan Hong

“…The expression of two key genes, ACTN4 and LCN2, was verified by immunohistochemistry and RT-qPCR.ConclusionThis study established a clinical prognostic model associated with disulfidptosis/ferroptosis-related genes, and the expression characteristics of key genes were validated in clinical samples. …”
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Combining Serum DNA Methylation Biomarkers and Protein Tumor Markers Improved Clinical Sensitivity for Early Detection of Colorectal Cancer by Guoying Zhang, Fang He, Guodong Zhao, Zihui Huang, Xiang Li, Xia Xia, Yidi Guo, Weiping Xu, Shangmin Xiong, Yong Ma, Minxue Zheng, Wanli Liu

“…The ColoDefense test, a multiplex qPCR assay simultaneously detecting both methylated SEPT9 and SDC2 genes, has demonstrated improved clinical performance on either methylation biomarker alone for CRC screening with both blood and stool samples. …”
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Discovery and in vitro characterization of a human anti-CD36 scFv by Cecilia Mata-Cruz, Cecilia Mata-Cruz, Sandra L. Guerrero-Rodríguez, Keyla Gómez-Castellano, Gregorio Carballo-Uicab, Juan Carlos Almagro, Juan Carlos Almagro, S. Mayra Pérez-Tapia, S. Mayra Pérez-Tapia, S. Mayra Pérez-Tapia, Marco A. Velasco-Velázquez

“…The phenotypic changes induced by CD36 ligands were assessed in vitro by: i) oil red staining, ii) tumorsphere assays, and iii) RT-qPCR.ResultsWe identified an anti-CD36 scFv, called D11, that competes with a commercial anti-CD36 antibody with proven efficacy in disease models. …”
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Expression pattern of Calbindin-D28k, NeuN proteins, ATOH1 and EN2 genes in the human fetal cerebellum by Phanindra Prasad Poudel, Arnab Ghosh, Chacchu Bhattarai, Saman Pradhan, Nirmal Panthi, Dela Singh Joshi, Shanti Khadka, Sandhya Kumari, Guruprasad Kalthur, R. Vani Lakshmi, Sneha Guruprasad Kalthur

“…Methods: This is a descriptive observational study carried out in human fetal cerebellum, fluorescent immunohistochemistry was performed to study the expression of Calbindin-D28k and NeuN, and while the expression of ATOH1 and EN2 genes were quantified with the help of qPCR. Results: Calbindin-D28k was highly immunoreactive in the Purkinje cells and located in their cytoplasm, nucleus and dendrites whereas absent in their axons. …”
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Elite Alleles of EPE1 Identified via Genome-wide Association Studies Increase Panicle Elongation Length in Rice by Hao Sun, Qiqi Yao, Mei Hai, Tianhu Li, Jinghan Sun, Zhengbo Liu, Yang Ang, Yingying Zhao, Yanan Zhang, Xianping Cheng, Tao Huang, Yinping Chang, Mingyu Du, Erbao Liu

“…Notably, our study identified a previously unreported QTL, qPE2.1. Three candidate genes associated with PEL were predicted by non-synonymous SNPs and functional annotation. …”
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Azithromycin Promotes the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells after Stimulation with TNF-α by Tingting Meng, Ying Zhou, Jingkun Li, Meilin Hu, Xiaomeng Li, Pingting Wang, Zhi Jia, Liyu Li, Dayong Liu

“…Real-time quantitative polymerase chain reaction (RT-qPCR) analysis was performed to detect the mRNA expression of osteogenic-related genes, including RUNX2, OCN, and BSP. …”
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Frequency of Trypanosoma cruzi Infection in Synanthropic and Wild Rodents Captured in a Rural Community in Southeast of Mexico by Ivonne Hernández-Cortazar, Karla Cecilia Amaya Guardia, Marco Torres-Castro, Karla Acosta-Viana, Eugenia Guzmán-Marín, José Israel Chan-Pérez, Antonio Ortega-Pacheco, Roger I. Rodríguez-Vivas, Rodrigo Medina-Pinto, Matilde Jiménez-Coello

“…DNA was extracted from samples to detect the presence of T. cruzi through quantitative PCR (qPCR). T. cruzi DNA was detected in the 9.75% of the blood samples of the synanthropic species (4/41) (14.28%) for Rattus rattus samples and 25% for Ototylomys phyllotis samples, with an average of parasitic load of 4.80 ± 1.17 parasites/μL. …”
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Comparative transcriptome analysis reveals potential regulatory mechanisms in response to changes in physiological functions in Oreochromis aureus under salinity stress by Chang Yuan, Kangqi Zhou, Xianhui Pan, Yong Lin, Junqi Qin, Dapeng Wang, Zhong Chen, Xuesong Du, Yin Huang

“…On the basis of GO, KEGG and STEM analyses, the data enabled the development of a mechanistic model that details how O. aureus adapts to salinity stress by regulating physiological changes. Finally, RT-qPCR assays verified the accuracy and reliability of the high-throughput sequencing results. …”
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Magnitude of consistent condom use and associated factors among people living with HIV/AIDS in Ethiopia: Implication for reducing infections and re-infection. A systematic review a... by Firomsa Bekele, Lalise Tafese, Teshome Debushe

“…The asymmetry of funnel plot and the Egger test and Begg's test P value of 0 >0.5 showed the absence of publication bias. The Cochrane Q test statistic and I2 tests were used to assess heterogeneity.…”
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Diversity in mitotic DNA repair efficiencies between commercial inbred maize lines and native Central American purple landraces by Carlos Víquez-Zamora, Sergio Castro-Pacheco, María Viñas, Pablo Bolaños-Villegas

“…Protein sequence analysis was done in UniProt to compare the resulting aminoacid sequences from maize to the available sequences from Arabidopsis thaliana ecotype Col-0 (ATM protein code: Q9M3G7). Results Single-cell electrophoresis results of statistical significance suggested that the landrace P1-Pujagua Santa Cruz is resistant to damage caused by the radiomimetic agent Zeocin, and landrace P2-Pujagua La Cruz was able to repair all DNA damage after 24 h of treatment and 1 h of recovery time. …”
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Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines by Valerio Pazienza, Francesca Tavano, Massimo Francavilla, Andrea Fontana, Fabio Pellegrini, Giorgia Benegiamo, Vincenzo Corbo, Fabio Francesco di Mola, Pierluigi Di Sebastiano, Angelo Andriulli, Gianluigi Mazzoccoli

“…We investigated the time-related patterns of PPARG, DNMT1, and DNMT3B expression monitoring their mRNA levels by qRT-PCR at different time points over a 28-hour span in BxPC-3, CFPAC-1, PANC-1, and MIAPaCa-2 PC cells after synchronization with serum shock. …”
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Linear Programming as a Tool for Managing the Training Process of Esports Teams by Oksana Shynkaruk, Nataliia Byshevets, Alla Aloshyna, Olena Iakovenko, Kostiantyn Serhiienko, Valerii Pinchuk, Oleksandr Petryk, Ivan Lut

“…Reliable statistical methods were employed: the dichotomous scale (results were processed using Cochran’s Q concordance coefficient, which determined the consistency of expert opinions regarding each type of training); and the ratio scale (ranking) – the consistency of opinions was analyzed using Kendall’s W concordance coefficient. …”
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The Petri Dish-N2B27 Culture Condition Maintains RPE Phenotype by Inhibiting Cell Proliferation and mTOR Activation by Hui Lou, Chunpin Lian, Fanjun Shi, Liqun Chen, Sicheng Qian, Hui Wang, Xiaoyun Zhao, Xiaoyan Ji, Jingfa Zhang, Guoxu Xu

“…Morphological and biological characteristics were investigated using light microscopy, Q-PCR, and western blot. Results. The retina would curl inwardly during the growth medium incubation period, releasing RPE sheets in the medium. …”
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Vitamin B12, folate, and homocysteine levels in children and adolescents with obesity: a systematic review and meta-analysis by Juan R. Ulloque-Badaracco, Esteban A. Alarcon-Braga, Enrique A. Hernandez-Bustamante, Enrique A. Hernandez-Bustamante, Beatrix M. Von-Koeller-Jones, Miguel Huayta-Cortez, Esduardo Saavedra-Custodio, Percy Herrera-Añazco, Vicente A. Benites-Zapata

“…Standardized mean difference (SMD) with the corresponding 95% CI was used as the only effect size. The Cochran’s Q test and the I2 statistic were used to evaluate between-study heterogeneity. …”
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Influence of Titanium Alloy Scaffolds on Enzymatic Defense against Oxidative Stress and Bone Marrow Cell Differentiation by Lais Morandini Rodrigues, Elis Andrade Lima Zutin, Elisa Mattias Sartori, Daniela Baccelli Silveira Mendonça, Gustavo Mendonça, Yasmin Rodarte Carvalho, Luana Marotta Reis de Vasconcellos

“…Quantitative real time PCR (qRT-PCR) was performed after 3, 7, and 14 days, and Runx2 (Runt-related transcription factor2) expression was evaluated. …”
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Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium s... by Ruyi Li, Songkun Lin, Yin Yan, Yuming Chen, Linying Wang, Yuzhen Zhou, Shuling Tang, Ning Liu

“…Transcriptomic data revealed that seven <i>CsSOD</i>s exhibited a border expression in all sequenced tissues, while two exhibited undetectable expression levels. Further qRT-PCR analysis showed that all <i>CsSOD</i>s were upregulated under salt stress, with some exhibiting significant changes in expression. …”
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