TLR4 Inhibition Attenuated LPS‐Induced Proinflammatory Signaling and Cytokine Release in Mouse Hearts and Cardiomyocytes by Christine W. Wiger, Trine Ranheim, Henriette Arnesen, Jarle Vaage, Søren E. Pischke, Arne Yndestad, Kåre‐Olav Stensløkken, May‐Kristin Torp

“…Cytokine expression was analyzed with ELISA, western blot analysis, and qPCR. Results In isolated hearts, E. coli increased the expression of proinflammatory cytokines (IL‐6 and CXCL2), which was not attenuated with TLR4 inhibition. …”
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Diagnostic Accuracy of Procalcitonin for Bacterial Infection in Liver Failure: A Meta-Analysis by Xinchun He, Liang Chen, Haiou Chen, Yuqing Feng, Baining Zhu, Caixia Yang

“…In the forest map, the DOR of each study and the combined DOR are not distributed along the same line, and Q = 2.2 × 1014, P≤0.001. Furthermore, the metaregression analysis of PCT study design, bacterial infection site, and mean age displayed that the P values were >0.05. …”
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Targeting CXCL8 signaling sensitizes HNSCC to anlotinib by reducing tumor-associated macrophage-derived CLU by Xin Hu, Yikang Ji, Mi Zhang, Zhihui Li, Xinhua Pan, Zhen Zhang, Xu Wang

“…In vivo and in vitro CXCL8 and CLU expression levels in tumor cells were determined using qPCR, immunohistochemistry and ELISA. The ability of CLU from TAMs to respond to tumor-derived CXCL8 was assessed using RNA sequencing, siRNA silencing, immunofluorescence and CCK-8 assays. …”
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Study on the effects of Mogroside V in inhibiting NLRP3-mediated granulosa cell pyroptosis and insulin resistance to improve PCOS by Wenqin Yang, Yujie Ma, Yafei Wu, Xiaocan Lei, Jing Zhang, Meixiang Li

“…Expression of pyroptosis and insulin resistance markers was analyzed using qRT-PCR, Western blot, and IHC. Additionally, an in vitro model assessed MV's impact on inflammation and insulin resistance. …”
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Bi-targeting of thioredoxin 1 and telomerase by thiotert promotes cell death of myelodysplastic syndromes and lymphoma by Qiangan Jing, Yunyi Wu, Yanchun Li, Chaoting Zhou, Junyu Zhang, Jun Xia, Keyi Li, Yuhuan Shen, Hongfeng Yao, Xiangmin Tong, Jing Du, Lushan Yu, Ying Wang

“…Meanwhile, pharmacological administration of glutathione (GSH), N-acetylcysteine (NAC), and mitoquinone (MitoQ), or genetic overexpression of TRX1 or TERT in MDS and cells could dampen the toxicity caused by thiotert. …”
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Comparison of Different Invasive Devices for the Treatment of Urinary Incontinence after Radical Prostatectomy by Stefano Salciccia, Pietro Viscuso, Giulio Bevilacqua, Antonio Tufano, Paolo Casale, Ettore De Berardinis, Giovanni Battista Di Pierro, Susanna Cattarino, Alessandro Gentilucci, Francesca Lourdes Lia, Di Giulio Ivan, Davide Rosati, Francesco Del Giudice, Alessandro Sciarra, Gianna Mariotti

“…Considering a random effect model among eligible studies, ER of continence recovery was 0.33 (95% CI −0.12–0.78), 0.63 (95% CI 0.55–0.71), 0.65 (95% CI 0.58–0.72), 0.50 (95% CI 0.34–0.66), and 0.53 (95%CI 0.36–0.70), respectively, in groups A, B, C, D, and E (I2 85.87%; Q 249.82—P>0.01) (test of group differences P=0.22). …”
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Emergency Physicians’ and Nurses’ Perspectives on Transgender, Intersexual, and Non-Binary Patients in Germany by Torben Brod, Carsten Stoetzer, Christoph Schroeder, Stephanie Stiel, Kambiz Afshar

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Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock: Downstream Regulation of COX-2, IL-1β,... by André L. F. Sampaio, Jesmond Dalli, Vincenzo Brancaleone, Fulvio D'Acquisto, Mauro Perretti, Carmen Wheatley

“…We used quantitative polymerase chain reaction (qPCR), ELISA, Western blot, and NOS Griess assays, in a C57BL/6 mouse, acute endotoxaemia model. …”
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Influence of nano-polystyrene on cyfluthrin toxicity in honeybee Apis cerana cerana Fabricius by Yunfei Xue, Xueyang Gong, Linfu Yang, Dandan Zhi, Qingxin Meng, Yulong Guo, Kun Dong, Yakai Tian

“…Concerning the detoxification-related genes, the two toxins alone and in combination significantly promoted the expression of P450 9E2 and Cyp9Q3 genes, and the upregulation trend was found more significant for the combination. …”
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Evaluating H2BC9 as a potential diagnostic and prognostic biomarker in head and neck squamous cell carcinoma by Lanhua Wu, Liang Li, Mingjing Zhu, Ziyan Zhou, Xuejin Su, Yueming Jiang, Min Kang, Li Jiang

“…H2BC9 expression was validated using the Human Protein Atlas database, qRT-PCR, and western blotting. Prognostic value was assessed using Kaplan–Meier curves, Cox regression analysis, and a nomogram. …”
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Atrial fibrillation in acute coronary syndrome: clinical and anamnestic features and effects on the course by V.І. Tseluyko, F.B. Salem, N.A. Lopina, I.V. Kuznetsov

“…Patients with SCS and AF are more likely to develop complications in the acute period and the formation of pathological Q wave in future…”
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The In Vitro Immunomodulatory Effects of Gold Nanocomplex on THP-1-Derived Macrophages by Duaa Abuarqoub, Nouf N. Mahmoud, Rand Zaza, Rana Abu-Dahab, Enam A. Khalil, Dima A. Sabbah

“…After treatment, the production of the inflammatory cytokines measured at gene and protein levels by using qPCR and CBA array beads by flow cytometry. Results. …”
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Protective Effects of NaHS/miR-133a-3p on Lipopolysaccharide-Induced Cardiomyocytes Injury by Yi-Mei Jin, Ai-Rong Huang, Mei-qian Yu, Wan-Ding Ye, Xiao-guang Hu, Hua-min Wang, Zhi-wei Xu, Dong-shi Liang

“…The adenosine triphosphate (ATP) kit was used to detect ATP content; real-time quantitative polynucleotide chain reaction (qPCR) was used to measure the levels of mammalian targets of rapamycin (mTOR), AMP-dependent protein kinase (AMPK), and miR-133a-3p, and Western blot (WB) was used to detect protein levels of mTOR, AMPK, myosin-like Bcl2 interacting protein (Beclin-1), microtubule-associated protein 1 light chain 3 (LC3I/II), and P62 (sequestosome-1, sqstm-1/P62). …”
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KLRB1 expression in nasopharyngeal mucosa as a prognostic biomarker in COVID-19 patients by Marilina García-Aranda, María Ángeles Onieva, Desirée Martín-García, Raúl Quirós, Inmaculada López, María Padilla-Ruiz, Teresa Téllez, Beatriz Martínez-Gálvez, María Luisa Hortas, Alberto García-Galindo, José González-Gomariz, Rubén Armañanzas, Francisco Rivas-Ruiz, Alfonso Serrano, Isabel Barragán-Mallofret, Maximino Redondo

“…These findings were validated using qRT-PCR in an independent cohort of 168 patients hospitalized in 2022. …”
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Group 2 Innate Lymphoid Cells Participate in Renal Fibrosis in Diabetic Kidney Disease Partly via TGF-β1 Signal Pathway by Cuiping Liu, Ludan Qin, Jingya Ding, Luping Zhou, Chenlin Gao, Ting Zhang, Man Guo, Wei Huang, Zongzhe Jiang, Yang Long, Yong Xu

“…The expression of transforming growth factor-β1 (TGF-β1), fibronectin (FN), collagen1, IL-4Rα, and IL-13Rα1 in renal tubular epithelial cells (HK-2) induced by IL-4, IL-13, or high glucose was analyzed by ELISA or qPCR. Results. The proportion of ILC2s and the levels of IL-4, IL-5, and IL-13 were significantly increased in DKD patients and were positively correlated with the severity of DKD (P<0.05). …”
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Circulating MicroRNAs in Patients with Psoriasis Treated with Anti-IL-23: A Cohort Study by Federico Diotallevi, Giulia Matacchione, Anna Campanati, Elena Marinelli Busilacchi, Nadia Viola, Ilaria Pace, Beatrice Fontana, Roberta Roncarati, Massimiliano Bonafè, Manuela Ferracin, Jacopo Sabbatinelli, Fabiola Olivieri

“…Findings were validated in a separate cohort of 23 patients using quantitative real-time PCR (qRT-PCR). T-regulatory cell (Treg) numbers and pro-inflammatory cytokine levels were also assessed. …”
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Exploring the Prevalence and Components of Metabolic Syndrome in Sub-Saharan African Type 2 Diabetes Mellitus Patients: A Systematic Review and Meta-Analysis by Nelson Musilanga, Hussein Nasib, Given Jackson, Frank Shayo, Clarkson Nhanga, Saleh Girukwigomba, Ambokile Mwakibolwa, Samson Henry, Keneth Kijusya, Edgar Msonge

“…Evidence of between-study variance attributed to heterogeneity was assessed using Cochran’s Q statistic and the I2 statistic. The Joanna Briggs Institute quality appraisal criteria were used to evaluate the methodological quality of the included studies. …”
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TLR7: A Key Prognostic Biomarker and Immunotherapeutic Target in Lung Adenocarcinoma by Feiming Hu, Chenchen Hu, Yuanli He, Yuanjie Sun, Chenying Han, Xiyang Zhang, Lingying Yu, Daimei Shi, Yubo Sun, Junqi Zhang, Dongbo Jiang, Shuya Yang, Kun Yang

“…Validation of TLR7 expression was conducted using GEO data, qPCR, WB, and IHC. A prognostic model was developed using a nomogram, incorporating TLR7 expression. …”
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Doxycycline and Benznidazole Reduce the Profile of Th1, Th2, and Th17 Chemokines and Chemokine Receptors in Cardiac Tissue from Chronic Trypanosoma cruzi-Infected Dogs by Guilherme de Paula Costa, Laís Roquete Lopes, Maria Cláudia da Silva, Aline Luciano Horta, Washington Martins Pontes, Cristiane M. Milanezi, Paulo Marcos da Mata Guedes, Wanderson Geraldo de Lima, Richard Schulz, João Santana da Silva, Andre Talvani

“…After 14 months of infection, hearts were excised and processed for qPCR analysis of Th1 (CCL2, CCL3, CCL4, CCL5, CXCL9, and CXCL11), Th2 (CCL1, CCL17, CCL24, and CCL26), Th17 (CCL20) CKs, Th1 (CCR5, CCR6, and CXCR3), and Th2/Th17 (CCR3, CCR4, and CCR8) CKR, as well as IL-17. …”
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Associations among circulating sphingolipids, β-cell function, and risk of developing type 2 diabetes: A population-based cohort study in China. by Huan Yun, Liang Sun, Qingqing Wu, Geng Zong, Qibin Qi, Huaixing Li, He Zheng, Rong Zeng, Liming Liang, Xu Lin

“…Network analysis further identified 5 modules, and 2 modules containing saturated sphingomyelins showed the strongest associations with increased T2D risk (RRQ4 versus Q1 = 1.59 and 1.43; both Ptrend < 0.001). Mediation analysis suggested that the detrimental associations of 13 sphingolipids with T2D were largely mediated through β-cell dysfunction, as indicated by HOMA-B (mediation proportion: 11.19%-42.42%; all P < 0.001). …”
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