Folic acid supplementation during preconception period in sub-Saharan African countries: A systematic review and meta-analysis. by Mekuriaw Nibret Aweke, Elsa Awoke Fentie, Muluken Chanie Agimas, Lemlem Daniel Baffa, Ever Siyoum Shewarega, Aysheshim Kassahun Belew, Esmael Ali Muhammad, Berhanu Mengistu

“…Heterogeneity was evaluated with Cochrane Q and I2 statistics, and small study effects were tested with Egger's test at a 5% significance level. …”
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An Unusual Case of Ototoxicity with Use of Oral Vancomycin by Umut Gomceli, Srija Vangala, Cosmina Zeana, Paul J. Kelly, Manisha Singh

“…Vancomycin was initiated at a dose of 125 mg po q6h. After the third dose of oral vancomycin, the patient reported new symptoms of lightheadedness, sensations of “buzzing” and whistling of bilateral ears, and decreased perception of hearing described as “clogged ears.” …”
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Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies by A. Seck, A. Hichami, S. Doucouré, F. Diallo Agne, H. Bassène, A. Ba, C. Sokhna, N. A. Khan, A. Samb

“…The mRNA expression of inflammatory markers in T cells was determined by RT-qPCR. We observed that diabetic mothers exhibited higher HbA1C, glycemia, insulinemia, TG, Tchol, HDLchol, and LDLchol levels than control mothers. …”
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Effect of the gut microbiome in glaucoma risk from the causal perspective by He Chen, Meng Zhou, Zicheng Zhang, Siqi Bao, Jia Qu, Yaxuan Wu, Ronghua Shi

“…Inverse variance weighted, MR-Egger, weighted median, weighted mode, Mendelian Randomisation Pleiotropy Residual Sum and Outlier, MR-Egger intercept and Cochran’s Q statistical analyses were used to supplement MR results and sensitivity analysis. …”
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Hereditary angioedema diagnosis evaluation score (HADES): A new clinical scoring system for predicting hereditary angioedema with C1 inhibitor deficiency by Ricardo Zwiener, MD, Rafael Zamora, MD, Carlos María Galmarini, MD, PhD, Laura Brion, PhD, Laura Arias, MD, Andrea Pino, MD, Paula Rozenfeld, PhD

“…Blood samples were analyzed for C1-INH/C1q levels and C1-INH function. A predictive score was developed from the odds ratios derived from multivariate logistic regression analysis. …”
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Genome-wide characterization and expression analysis of the ADF gene family in response to salt and drought stress in alfalfa (Medicago sativa) by Mengmeng Shi, Yike Wang, Peng Lv, Yujie Gong, Qi Sha, Xinyan Zhao, Wen Zhou, Lingtao Meng, Zegang Han, Lingxiao Zhang, Lingxiao Zhang, Yongwang Sun, Yongwang Sun

“…Gene expression profiles analyzed by RT-qPCR experiments demonstrated that MsADF genes exhibited distinct expression patterns among different organs. …”
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Exploring MAP3K genes in gastric cancer: biomarkers, tumor microenvironment dynamics, and chemotherapy resistance by Senhui Wei, Ying Li, Jing Zhou, Yongming Xia

“…Methods In this study, we investigated the expression profiles and clinical implications of MAP3K genes in GC using in silico and in vitro experiments. Results Utilizing RT-qPCR analysis, we observed significant up-regulation of MAP3K1, MAP3K4, MAP3K5, MAP3K6, MAP3K7, MAP3K8, MAP3K9, and MAP3K10 in GC cell lines, while MAP3K2, MAP3K3, MAP3K11, MAP3K12, MAP3K13, MAP3K14, and MAP3K15 exhibited down-regulation. …”
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Calcium hydroxide nanoparticles induce cell death, genomic instability, oxidative stress and apoptotic gene dysregulation on human HepG2 cells by Hanan R. H. Mohamed, Esraa H. Ibrahim, Shahd E. E. Shaheen, Nesma O. E. Hussein, Ayman Diab, Gehan Safwat

“…Reactive oxygen species (ROS) levels were measured using 2,7-dichlorofluorescein diacetate, while the expression level of apoptosis-related genes (p53, Bax, and Bcl-2) were quantified using real-time PCR (qRT-PCR). The SRB cytotoxicity assay revealed that a 48-hour exposure to Ca(OH)2NPs caused concentration-dependent cell death and proliferation inhibition in both HSF and HepG2 cells, with IC50 values of 271.93 µg/mL for HSF and 291.8 µg/mL for HepG2 cells. …”
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Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration by Andrea Barragán-Cárdenas, Daniel Castellar-Almonacid, Yerly Vargas-Casanova, Claudia Parra-Giraldo, Adriana Umaña-Pérez, Joel López-Meza, Zuly Rivera-Monroy, Javier García-Castañeda

“…Evaluation of mechanism associated to the cytotoxic effect were carried out by flow cytometry, RT-qPCR, wound healing, transwell and zymography. …”
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Correlations among PPAR𝜸, DNMT1, and DNMT3B Expression Levels and Pancreatic Cancer by Valerio Pazienza, Francesca Tavano, Giorgia Benegiamo, Manlio Vinciguerra, Francesca Paola Burbaci, Massimiliano Copetti, Fabio Francesco di Mola, Angelo Andriulli, Pierluigi di Sebastiano

“…In this study we aimed to evaluate the expression of PPARγ, DNMT1, and DNMT3B and their correlation with clinical-pathological features in patients with pancreatic cancer (PC), and to define the effect of PPARγ activation on DNMTs expression in PC cell lines. qRT-PCR analysis showed that DNMT3B expression was downregulated in tumors compared to normal tissues (𝑃=0.03), whereas PPARγ and DNMT1 levels did not show significant alterations in PC patients. …”
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Modelling of a new form of nitrogen doped activated carbon for adsorption of various dyes and hexavalent chromium ions by Mohamed A. El-Nemr, Uyiosa Osagie Aigbe, Kingsley Eghonghon Ukhurebor, Kingsley Obodo, Adetunji Ajibola Awe, Mohamed A. Hassaan, Safaa Ragab, Ahmed El Nemr

“…The determined maximum sorption capacity (Q m) based on the NLM was 1114, 1929 and 318 mg.g-1 for AB14 dye, AO7 dye and Cr6+ ions, respectively. …”
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Analysis of the IGS rRNA Region and Applicability for Leishmania (V.) braziliensis Characterization by Tayná C. de Goes, Rayana C. S. de Morais, Maria G. N. de Melo, Rômulo P. e Silva, Antônio M. Rezende, Milena de Paiva-Cavalcanti

“…In addition, a set of primers for L. braziliensis identification for qPCR was developed and optimized. Sensitivity (S), specificity (σ), and efficiency (ε) tests were applied. …”
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Correlation of Surface Toll-Like Receptor 9 Expression with IL-17 Production in Neutrophils during Septic Peritonitis in Mice Induced by E. coli by Yunjia Ren, Li Hua, Xiuping Meng, Yue Xiao, Xu Hao, Sheng Guo, Peiyan Zhao, Luowei Wang, Boqi Dong, Yongli Yu, Liying Wang

“…Using experiments of in vitro blockage, qRT-PCR and cell sorting, we confirmed that PMNs in the PLCs did increase their IL-17 expression during E. coli infection. …”
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RNA-Seq-Based Gene Expression Pattern and Morphological Alterations in Chick Thymus during Postnatal Development by Zhouyiyuan Xue, Abdur Rahman Ansari, Xing Zhao, Kun Zang, Yu Liang, Lei Cui, Yafang Hu, Ranran Cheng, Xiaolong Zhang, Juming Zhong, Huazhen Liu

“…The expression pattern revealed by RNA-seq was validated by quantitative real-time PCR (qPCR) analysis of four important genes, which are PCNA, CCNA2, CCNB2, and CDK1. …”
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Bioinformatics insights into mitochondrial and immune gene regulation in Alzheimer's disease by Tian Meng, Yazhou Zhang, Yuan Ye, Hui Li, Yongsheng He

“…After platform removal, there was a notable increase in the path complexity and time required to reach the target quadrant, suggesting a reduction in spatial memory capacity in AD mice. Moreover, qRT-PCR validation confirmed that the mRNA expression of the five biomarkers was consistent with bioinformatics results. …”
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Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis by Jiaqi Ai, Wuhong Wang, Tianhua Hu, Haijiao Hu, Yaqin Yan, Jinglei Wang, Yunzhu Wang, Na Hu, Hongtao Pang, Chonglai Bao, Qingzhen Wei

“…The quantitative real-time PCR (qRT-PCR) analysis results showed that SmeMYB175 was regarded as the most likely key transcription factor regulating anthocyanin synthesis in calyx. …”
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Evaluation of the diagnostic accuracy of two point-of-care tests for COVID-19 when used in symptomatic patients in community settings in the UK primary care COVID diagnostic accura... by Brian D Nicholson, Philip J Turner, Thomas R Fanshawe, Alice J Williams, Gayatri Amirthalingam, Sharon Tonner, Maria Zambon, Richard Body, Kerrie Davies, Rafael Perera, Simon de Lusignan, Gail N Hayward, F D Richard Hobbs, RAPTOR-C19 Study Group and the CONDOR Steering Committee

“…Participants were tested by trained healthcare workers using at least one of two index POCTs (Roche-branded SD Biosensor Standard™ Q SARS-CoV-2 Rapid Antigen Test and/or BD Veritor™ System for Rapid Detection of SARS-CoV-2). …”
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Dynamic Complement Protein Changes in Aqueous Humor and Plasma of Patients With Retinal Vein Occlusion During Ranibizumab Treatment by Guo T, Zhao Y, Liang S, Wang J, Liu H, Zhou Y, Xu H, Chen Z

“…The concentrations of 13 complement proteins and vascular endothelial growth factor A (VEGF-A) were measured using Luminex® × MAP® technology.Results: During ranibizumab treatment, a reduction in the levels of C1q (p < 0.001), C2 (p = 0.030), C4 (p = 0.001), C4b (p = 0.026), C3b/iC3b (p < 0.001), C5 (p = 0.007), C5a (p = 0.005), CFD (p = 0.022), CFH (p < 0.001), and CFI (p < 0.001) in AH was observed. …”
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Genetic Analysis of in Korean Patients Associated with Atrioventricular Conduction Block by Hyoung Seob Park, Yoon Nyun Kim, Young Soo Lee, Byung Chun Jung, Sang Hee Lee, Dong Gu Shin, Yongkeun Cho, Myung Hwan Bae, Sang Mi Han, Myung Hoon Lee

“…Of the 7 variations, 5 (G87A-A29A, intervening sequence 9-3C>A, A1673G-H558R, G3578A-R1193Q, and T5457C-D1819D) have been reported in previous studies, and 2 (C48G-F16L and G3048A-T1016T) were novel variations that have not been reported. …”
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Comprehensive analysis of the circRNA expression profile and circRNA-miRNA-mRNA network in pelvic organ prolapse by Qian Wang, Zuoxi He, Lisha Ding, Yuqing Liu, Xiaoli Zhang, Tao Wang, Xiaoyu Niu

“…A total of 75 differentially expressed circRNAs (DEcircRNAs) were identified (fold change &gt;2.0, P &lt; 0.05). Furthermore, RT-qPCR confirmed that the expression levels of two circRNAs (hsa_circ_0067962 and hsa_circ_0057051) were significantly lower in the POP group. …”
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