Predictive value of dendritic cell-related genes for prognosis and immunotherapy response in lung adenocarcinoma by Zihao Sun, Mengfei Hu, Xiaoning Huang, Minghan Song, Xiujing Chen, Jiaxin Bei, Yiguang Lin, Size Chen

“…Expression of key genes in clinical samples was examined by real-time q-PCR. Performance of the prognostic model, DCRGS, for the prognostic evaluation, was assessed using a multiple time-dependent receiver operating characteristic (ROC) curve, the R package, “timeROC”, and validated using GEO datasets. …”
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Development and validation of a LAMP-based method for rapid and reliable detection of Xanthomonas albilineans, the causal agent of sugarcane leaf scald by Moutoshi Chakraborty, Moutoshi Chakraborty, Shamsul Arafin Bhuiyan, Shamsul Arafin Bhuiyan, Simon Strachan, Simon Strachan, Muhammad J. A. Shiddiky, Nam-Trung Nguyen, Rebecca Ford, Rebecca Ford

“…Quantification of Xalb was accurately correlated with sugarcane cultivar disease ratings. Validation using qPCR showed 91–98% agreement. This assay also effectively determined optimal sampling times and plant parts by monitoring the progression of the disease over time.DiscussionThis diagnostic assay holds significant potential as a commercial opportunity for a kit-based DNA extraction/purification-free molecular detection alternative. …”
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Potential Value of Circular RNA circTBC1D4 in Gastrointestinal Stromal Tumors by null Chunyan-Zou, null Yanpeng-He, null Changshun-Xie, null Fulai-Gao, null Xiaoli-Xie, null Xiukun-Chai, null Chenxi-He, null Dongqiang-Zhao

“…Quantitative real-time PCR (qRT-PCR) was used to measure the expression levels of hsa-circ-0002917 (circTBC1D4), hsa-miR-590-5p (miR-590-5p), and PLN. …”
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Impact of Prone Position on 12-Lead Electrocardiogram in Healthy Adults: A Comparison Study with Standard Electrocardiogram by Yunis Daralammouri, Murad Azamtta, Hamza Hamayel, Amro Adas, Osama Sawalmeh, Yahia Ismail, Saed H. Zyoud

“…In addition, changes in the QRS morphology in leads V1–V3 with the appearance of new Q waves were noted. A notable variation in the mean corrected QT (QTc) period with decrease in duration in prone posture ECG (385 ± 64.8) relative to supine (406 ± 18.8, p=0.05). …”
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Genome-wide association study identifies four pan-ancestry loci for suicidal ideation in the Million Veteran Program. by Allison E Ashley-Koch, Nathan A Kimbrel, Xue J Qin, Jennifer H Lindquist, Melanie E Garrett, Michelle F Dennis, Lauren P Hair, Jennifer E Huffman, Daniel A Jacobson, Ravi K Madduri, Hilary Coon, Anna R Docherty, Jooeun Kang, Niamh Mullins, Douglas M Ruderfer, VA Million Veteran Program (MVP), MVP Suicide Exemplar Workgroup, International Suicide Genetics Consortium, Philip D Harvey, Benjamin H McMahon, David W Oslin, Elizabeth R Hauser, Michael A Hauser, Jean C Beckham

“…Gene-set analysis implicated synaptic and startle response pathways (q's<0.05). European ancestry (EA) analysis identified GWS loci on chromosomes 6 and 9, as well as GWS gene associations in EXD3, DRD2, and DCC. …”
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Genes related to neural tube defects and glioblastoma by Rui Cao, Yurong Liu, Kaixin Wei, Ning Jin, Yuxiang Liang, Ruifang Ao, Weiwei Pan, Xiang Wang, Xiuwei Wang, Li Zhang, Jun Xie

“…We performed bioinformatics analysis on transcriptome sequencing data of retinoic acid (RA)-induced NTDs mice, human NTDs samples and GBM samples. RT-qPCR, Western blot, and immunohistochemistry were used to validate the expression of candidate genes. …”
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Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells by Antonella Bordin, Francesca Pagano, Eleonora Scaccia, Matteo Saccucci, Iole Vozza, Noemi Incerti, Antonella Polimeni, Elena Cavarretta, Isotta Chimenti, Elena De Falco

“…Cultures were also induced to differentiate towards the osteogenic lineage, and ALP and osteocalcin gene expression levels were evaluated by real-time qPCR. Results have shown that the number of clones generated by DPSCs exposed to T2D oral plaque was significantly lower compared to controls (ctl). …”
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Role of Aging in Ulcerative Colitis Pathogenesis: A Focus on ETS1 as a Promising Biomarker by Ni M, Peng W, Wang X, Li J

“…Finally, the expression levels of key genes were determined in a dextran sulfate sodium (DSS)-induced UC mouse model by qRT-PCR.Results: UC samples were classified into two subtypes (1 and 2), which displayed significant differences in the immune landscape and JAK/STAT signaling pathways. …”
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Ionizing radiation and photodynamic therapy lead to multimodal tumor cell death, synergistic cytotoxicity and immune cell invasion in human bladder cancer organoids by Annabell Reinhold, Annegret Glasow, Sandra Nürnberger, Annett Weimann, Lucie Telemann, Jens-Uwe Stolzenburg, Jochen Neuhaus, Mandy Berndt-Paetz

“…The cell death mode was analyzed by applying specific inhibitors followed by immunofluorescence or qPCR analyses of cell death markers. A matrix-based co-culture model was used to study T cell migration into the environment of treated organoids. …”
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Exploring the anti-metastatic potential of sunitinib and novel analogs in colorectal cancer: insights into HIF-1α mediated metastasis by Fatemeh B. Rassouli, Maryam M. Matin, Maryam M. Matin, Farzin Hadizadeh, Farzin Hadizadeh, Masoud Nejabat, Hossein Allahverdizadeh, Hamidreza Jamali, Shahin Gharedaghi, Halimeh Hassanzadeh

“…Gelatin zymography and qPCR analysis indicated decreased activity of MMP-2 and MMP-9, along with downregulation of EMT transcription factors in hypoxic condition. …”
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Exploring the antiviral potential of shikimic acid against Chikungunya virus through network pharmacology, molecular docking, and in vitro experiments by Jialiang Xin, Xingxing Song, Haohong Zheng, Wenjing Li, Yuyang Qin, Wei Wang, Wei Wang, He Zhang, Guangneng Peng

“…Additionally, SA was found to markedly downregulate the expression of CHIKV-related attachment factors ACTG1, TSPAN9, and TIM-1 in 293T cells infected with CHIKV. Furthermore, RT-qPCR analysis demonstrated that SA effectively decreased the expression of NFKB1, PTGS2, RELA, and EGF related to the IL-17 signaling pathway. …”
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Sustainable CO₂ Capture: N,S-Codoped Porous Carbons Derived from Petroleum Coke with High Selectivity and Stability by Jiawei Shao, Yingyi Wang, Mingyang Che, Ya Liu, Yongfu Jiang, Qiang Xiao, Muslum Demir, Linlin Wang, Xin Hu

“…Thermodynamic analysis indicated moderate isosteric heat of adsorption (<i>Q_st</i>) values ranging from 18 to 47 kJ/mol, ensuring both strong adsorption and efficient desorption. …”
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Asthma and obesity increase inflammatory markers in children by Harshita Shailesh, Safa Noor, Lena Hayati, Antonisamy Belavendra, Nicholas Van Panhuys, Abdul Badi Abou-Samra, Stefan Worgall, Ibrahim Janahi, Ibrahim Janahi

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Dufulin Impacts Plant Defense Against Tomato Yellow Leaf Curl Virus Infecting Tomato by Liping Huang, Yingying Tang, Shuaixin Wang, Jianbin Chen, Jiao Du, Shuo Yan, Deyong Zhang, Xiaobin Shi, Yong Liu, Fan Li

“…Additionally, this study assessed the expression levels of genes associated with systemic acquired resistance (SAR), specifically <i>proteinase inhibitor II</i> (<i>PI II</i>) and <i>non-expressor of pathogenesis-related genes 1</i> (<i>NPR1</i>), using real-time qRT-PCR. The chlorophyll and nitrogen content in the leaves were also measured. …”
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Mutational Analysis of KCNQ1 Gene in Type 2 Diabetes Mellitus: A Case-control Study by Ankush Ganjewar, Ishwar B Bagoji, Sharan Badiger, Rudragouda B Bulagouda, Gurushnatappa Kadakol

“…Introduction: KCNQ1 (Potassium voltage-gated channel subfamily Q member 1) gene encodes for pore-forming subunit of a voltage-gated K+ channel that is essential for the depolarisation phase of the action potential in cardiac muscle. …”
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GelMA@LNP/AST Promotes eNOS-Dependent Angiogenesis Through Autophagy Activation for the Treatment of Hind Limb Ischemia by Lai L, Wu H, Peng L, Zhang Z, Wu X, Zheng S, Su Z, Chu H

“…The changes in angiogenesis markers, autophagy markers, and eNOS levels were detected using qPCR and Western blotting. 3-MA was used to assess the role of autophagy in the activation of eNOS mediated by AST and its subsequent angiogenic effects.Results: GelMA@LNP/AST significantly promoted blood flow recovery in mice with hind limb ischemia. …”
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Describing viewpoints on human‐nature relationships to unveil socio‐environmental conflicts and support community‐based projects by Beatriz Demasi, Nina Garcia de Almeida Prado, Flávia Pereira Lima, Charbel N. El‐Hani, Renata Pardini

“…We developed a tool to describe viewpoints on human‐nature relationships that underpin socio‐environmental conflicts based on a multicultural conceptual framework and Q‐methodology. To investigate its usefulness to unveil conflicts within community‐based projects, we applied it to community members and researchers working collaboratively in an artisanal fishing community in Brazil. …”
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FoxA1 knockdown promotes BMSC osteogenesis in part by activating the ERK1/2 signaling pathway and preventing ovariectomy-induced bone loss by Lijun Li, Renjin Lin, Yang Xu, Lingdi Li, Zhijun Pan, Jian Huang

“…The expression of genes involved in osteogenic differentiation was examined via qRT‒PCR. Lentiviral transfection was utilized to suppress the expression of the FOXA1 gene in hBMSCs. …”
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Toward High-order Solar Corona Simulations: A High-order Hyperbolized Poisson Approach for Magnetic Field Initialization by Rayan Dhib, Firas Ben Ameur, Vatsalya Sharma, Andrea Lani, Stefaan Poedts

“…Our contributions include developing a Q 2 (i.e., quadratic) geometrical representation using prismatic elements for the computational domain, which enables high-order mesh generation. …”
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Impact of Amarogentin on Gastric Carcinoma Cell Multiplication, Apoptosis and Migration via circKIF4A/miR-152-3p by Zhi Tan, Weining Wang, Jin Peng, Zhen Zhou, Jia Pan, Aiming Peng, Hui Cao, Wenling Fan

“…To investigate the impact of Amarogentin (AG) on GC cell multiplication, apoptosis and migration and the possible mechanisms. Methods. qRT-PCR quantification of circKIF4A and miR-152-3p in GC tissues and normal counterparts as well as HGC-27 (human GC cell strain) and GES-1 (human gastric mucosal epithelial cell strain) was performed. …”
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