Exosomes Derived from CXCR4-Overexpressing BMSC Promoted Activation of Microvascular Endothelial Cells in Cerebral Ischemia/Reperfusion Injury by Xutong Li, Ye Zhang, Yong Wang, Dan Zhao, Chengcheng Sun, Shaoting Zhou, Dongsheng Xu, Jing Zhao

“…Exosomes derived from BMSCCXCR4 (ExoCXCR4) were isolated and characterized by transmission electron microscopy and dynamic light scattering. Western blot and qPCR were used to analyze the expression of CXCR4 in BMSCs and exosomes. …”
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Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident by Syun Tokita, Ryo Nakayama, Yohei Fujishima, Valerie Swee Ting Goh, Donovan Anderson, Ippei Uemura, Hikari Ikema, Jin Shibata, Yoh Kinoshita, Yoshinaka Shimizu, Hisashi Shinoda, Jun Goto, Maria Grazia Palmerini, Abdulla Mohamed Hatha, Takashi Satoh, Akifumi Nakata, Manabu Fukumoto, Tomisato Miura, Hideaki Yamashiro

“…Conversely, in the non‐breeding season, Fmo2 and Fmo2 (highly similar) were significantly upregulated in radiation‐exposed Fukushima mice. qPCR analysis results were consistent with transcriptome sequencing, detecting Lsp1 and Ptprk regulation in the testes of Fukushima mice. …”
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The association between eight complete blood count-derived inflammatory markers and muscle health by Jingyuan Zhang, Yuqi Wang, Heng Liu, Zhuolin Lei, Shouquan Cheng, Hong Cao

“…However, NMLR and NLR were significantly associated with changes in muscle mass only in Q4 (P < 0.05). In the stratified analysis by body mass index (BMI), only the SII, NLR, and NMLR were unaffected by BMI. …”
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REDD1 mediates HDM-induced nuclear-cytoplasmic translocation and release of IL-33 in airway epithelial cells by downregulating Nrf2 by Tian Luo, Wentao Ji, Yuxin Gong, Lichang Chen, Chao Liu, Dandan Zhang, Xi Li, Yanhua Lv

“…The relationship between varying levels of REDD1 expression, Nrf2, and related inflammatory factors was assessed using Western blot and qPCR. To further investigate the role of the REDD1-Nrf2-IL-33 axis in the development of asthma, we employed Nrf2 activators and inhibitors to reassess the impact of REDD1 on IL-33. …”
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Next-generation calcium oxide nanoparticles: A breakthrough in energy storage and humidity sensing by Asif Khan, Syed Tasleem Hussain, Abdul Naeem, Ayesha Sadiqa, Awais Ahmad, Muhammad Aamir Ali Shehzada, Munirah D. Albaqami

“…Humidity sensor measurements were performed by determining changes in resistance of the sensing material applying LCR (Q) meter due to changes in humidity levels in a close chamber. …”
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Downregulation of Long Noncoding RNA LINC00261 Attenuates Myocardial Infarction through the miR-522-3p/Trinucleotide Repeat-Containing Gene 6a (TNRC6A) Axis by Chaoxin Jiang, Qing Zhao, Chenlong Wang, Minyan Peng, Guoqing Hao, Zhifeng Liu, Wenjin Fu, Kewei Zhao

“…This study is aimed at investigating the expression of long noncoding RNA (lncRNA) LINC00261 in MI and its effect on myocardial cells. Methods. qRT-PCR was performed to detect the expression levels of LINC00261, miR-522-3p, and TNRC6A in normal and MI cells. …”
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Thermal and Aerodynamic Researches of Staggered Bundles for Choice of an Effective Spacing of Round-Finned Tubes by V. B. Kuntysh, A. B. Sukhotskii, G. S. Marshalova, V. V. Dudarev, V. N. Farafontov

“…The results of variant thermal and aerodynamic calculations with the use of the obtained data established the technical and economic feasibility of placing tubes at the vertices of an isosceles triangle with spacing S1 = 68–69 mm and S2 = 55 mm with failure to use the location of the tubes along an equilateral triangle with S1 = S2' = 64 mm (where S2' – is diagonal spacing). With Q = idem and other conditions being equal, the number of tubes on AVO decreases by 5.7 % with a decrease in power consumption to 4.0 %.…”
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The in vitro anticancer effects of FS48 from salivary glands of Xenopsylla cheopis on NCI-H460 cells via its blockage of voltage-gated K+ channels by Xiong Weichen, Fan Huizhen, Zeng Qingye, Deng Zhenhui, Li Guanhui, Lu Wancheng, Zhang Bei, Lai Shian, Chen Xin, Xu Xueqing

“…The present study aims to reveal the effects of FS48 on the Kv channels and the NCI-H460 human lung cancer cells through patch clamp, MTT, wound healing, transwell, gelatinase zymography, qRT-PCR and WB assays. The results demonstrated that FS48 can be effective in suppressing the Kv currents, migration, and invasion of NCI-H460 cells in a dose-dependent manner, despite the failure to inhibit the proliferation. …”
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Expectativas acerca das atividades síncronas na formação a distância do Programa Mais Médicos para o Brasil by Inara Pereira da Cunha, Sílvia Helena Mendonça de Moraes, Adriana Carvalho dos Santos, Débora Dupas Gonçalves do Nascimento

“…As respostas dos 722 estudantes e dos 72 tutores foram analisadas pelo software IRaMuTeQ, com tratamento lexical, classificação hierárquica descendente (CHD), análise fatorial por correspondência (AFC) e análise de similitude. …”
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Metformin Ameliorates Ulcerative Colitis Through Inhibiting NLRP3 Inflammasome Activation by Cao R, Jing J, Ma Y, Qi W, Huang X, Zhang C, Lu Z, He J, Wang G, Ma Y, Zhang H

“…Enzyme-linked immunosorbent assay (ELISA), Reverse transcription - quantitative polymerase chain reaction (RT-qPCR), Western blotting (WB), immunohistochemistry, and immunofluorescence were used to detect NLRP3 inflammasome activation in vivo. …”
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Autophagy inhibitor 3-methyladenine mitigates the severity of colitis in aged mice by inhibiting autophagy by Ailing Liu, Hongying Wang, Hong Lv, Hong Yang, Yue Li, Jiaming Qian

“…The levels of TNF -α, IL-6, CCL4, CXCL12 and CD86 were measured by qRT-PCR. The transcriptional activity of NF-κB was measured by electrophoretic mobility shift assay (EMSA). …”
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Over-Expression of MicroRNA-210 and MicroRNA-185-5p in Normotensive Late-Fetal Growth Restriction: Preliminary Cohort Study by Esra Altan Erbilen, Gulizar Fusun Varol, Necdet Sut, Nebiye Pelin Turker, Niyazi Cenk Sayin

“…We determined these two miRNAs using Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) and statistically analyzed them with clinical data to determine robustness. …”
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High hemolytic activity in Staphylococcus aureus t1081/ST45 due to increased hla protein production and potential RNAIII-independent regulation by Yu-Tzu Lin, Ngoc-Niem Bui, Yu-Syuan Cheng, Cheng-Wen Lin, Chun-Li Lee, Tai-Fen Lee, Po-Ren Hsueh

“…The expression of hla and its regulators RNAIII, mgrA, and saeR was analyzed via qRT‒PCR, while Hla protein levels were measured via Western blotting. …”
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Increased Co-Expression of PD-L1 and CTLA-4 Predicts Poor Overall Survival in Patients with Acute Myeloid Leukemia Following Allogeneic Hematopoietic Stem Cell Transplantation by Chen C, Qiu K, Chen J, Wang S, Zhang Y, Wang C, Li Y

“…This study aims to investigate the significance of the alteration of IC co-expression for evaluating the prognosis of AML patients following allogeneic hematopoietic stem cell transplantation (allo-HSCT).Patients and Methods: Quantitative real-time PCR (qRT-PCR) data of bone marrow (BM) samples from 62 de novo AML patients, including 37 patients who received allo-HSCT and 25 patients who received chemotherapy only, were used for prognostic analysis.Results: High expression of PD-1, PD-L1, PD-L2, CTLA-4, and LAG-3 was associated with poor overall survival (OS) in AML patients receiving allo-HSCT, while the expression levels of PD-1, PD-L2, CTLA-4, and LAG-3, other than PD-L1, were not significantly correlated with OS in AML patients receiving chemotherapy. …”
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Curdione combined with borneol treats bacterial mixed HPV infection by regulating the crosstalk among immune cells by Jingwei Liu, Jingwei Liu, Tong Shu, Yiheng Mu, Wanlin Zheng, Xiaohuan Lu, Xiaohuan Lu, Hong Tao, Hong Tao

“…Bioinformatics and network pharmacology approaches were employed to identify therapeutic targets of Baofukang suppository for BV/HPV co-infections. qRT-PCR, Western blot, immunofluorescence staining, and flow cytometry were utilized to validate the therapeutic targets of curdione and borneol, along with the associated immune molecular changes. …”
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Characterization of polyethylene and polyurethane microplastics and their adsorption behavior on Cu2+ and Fe3+ in environmental matrices by Ilaria Zanoni, Lucia Briccolani, Lara Faccani, Magda Blosi, Simona Ortelli, Matteo Crosera, Giovanna Marussi, Stefania Albonetti, Anna Luisa Costa

“…We observed selective adsorption for Cu2⁺ ions, which was both medium-dependent (more ions adsorbed in Elendt M7) and plastic-dependent, with PU showing a stronger affinity for Cu2⁺ in MilliQ and Egg water. On the contrary, both plastics showed similar adsorption capacity for Fe3⁺ ions across all media.…”
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Impact of Charge on Strange Compact Stars in Rastall Theory by Malick Sallah, Muhammad Sharif

“…For a fixed bag constant, <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi mathvariant="script">B</mi><mo>=</mo><mn>60</mn><mspace width="3.33333pt"></mspace><msup><mrow><mi>MeV</mi><mo>/</mo><mi>fm</mi></mrow><mn>3</mn></msup></mrow></semantics></math></inline-formula>, and two sets of the Rastall and charge parameters, <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>ζ</mi><mo>=</mo><mn>0.255</mn><mo>,</mo><mn>0.259</mn></mrow></semantics></math></inline-formula> and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mover accent="true"><mi mathvariant="script">Q</mi><mo stretchy="false">˜</mo></mover><mo>=</mo><mn>0.2</mn><mo>,</mo><mn>0.7</mn></mrow></semantics></math></inline-formula>, respectively, we analyze the consistency of the matter variables in the model and other physical parameters such as energy conditions, stellar mass, compactness, and surface redshift. …”
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Plasma Metabolite Profiles of Healthy Volunteers after Administration of a Thai Herbal Formula for Dizziness by Ranida Boonrak, Kajee Pilakasiri, Suksalin Boonranasubkajorn, Natchagorn Lumlerdkij, Pravit Akarasereenont

“…Phytochemical and metabolite profiling were performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and LC/MS Q-ToF, respectively, on plasma samples from 90 healthy participants aged 20 to 60 years. …”
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High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> Buren by Xinyi Jiang, Jiacheng Shen, Peng Lin, Youming Hou

“…The expression patterns of these six CYPs were analyzed by RT-qPCR, which revealed that <i>SinvCYP6K1</i> and <i>SinvCYP4V2</i> were only highly expressed in the antennae of adult workers. …”
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Treadmill training improves lung function and inhibits alveolar cell apoptosis in spinal cord injured rats by Xianbin Wang, Yingxue Fu, Xianglian Yang, Yan Chen, Ni Zeng, Shouxing Hu, Shuai Ouyang, Xiao Pan, Shuang Wu

“…Various techniques including arterial blood gas analysis, lung wet/dry weight ratio, HE staining, immunofluorescence staining, immunohistochemical staining, qRT-PCR, and Western blotting were employed to assess alterations in lung function and the expression levels of crucial apoptosis-related factors. …”
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