Unique Case Reports Associated with Ovarian Failure: Necessity of Two Intact X Chromosomes by Lakshmi Rao Kandukuri, Venkata Padmalatha, Murthy Kanakavalli, Raseswari Turlapati, Mangalipally Swapna, Metuku Vidyadhari, Govindaraghavan Saranaya, Kattera Himaja, Mamata Deenadayal, Bipin Kumar Sethi, Prasun Deb, Nalini Gupta, Baidyanath Chakraborthy, Pratibha Nallari, Lalji Singh

“…Interestingly, majority of the abnormalities involved the X-chromosome and we report two unique abnormalities, (46,XXdel(Xq21-22) and q28) and (mos,45XO/46,X+ringX) involving X chromosome in association with ovarian failure. …”
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Antenatal depression among pregnant women in Ethiopia: An umbrella review. by Mesfin Abebe, Yordanos Sisay Asgedom, Amanuel Yosef Gebrekidan, Tsion Mulat Tebeje

“…Study quality was assessed with the AMSTAR tool, and data extraction and analysis were performed using Microsoft Excel 2016 and STATA 14.0. The I2 and Cochran's Q tests were used to assess heterogeneity. Pooled effect sizes were calculated based on the pooled prevalence of antenatal depression and odds ratios for associated factors, with a 95% confidence interval indicating statistical significance.…”
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Geographic Distribution of Vaccinia Virus, Diagnosis and Demographic Aspects of Affected Populations, Minas Gerais, Brazil, 2000–2023 by Pedro H. B. e Silva, Maycon D. de Oliveira, Iara M. de Almeida, Iago J. S. Domingos, Ana G. Stoffella-Dutra, Galileu Barbosa Costa, Jaqueline S. de Oliveira, Felipe C. M. Iani, Márcio R. de Castro, Jonatas S. Abrahão, Erna G. Kroon, Giliane de S. Trindade

“…Laboratory findings indicate high levels of anti-OPXV antibodies in most individuals, with some showing acute infections confirmed by qPCR testing. This analysis informs public health policies, emphasizing the need for enhanced surveillance of and preventive measures for dairy farming communities.…”
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Experimental study of the piezoelectricity of Middle Pleistocene loess in the Weibei region, China by Yanlin JING, Jie LIU, Qingying LIU, Xiaoxiao DUAN, Na XIE, Dengke YANG

“…MethodsThis study investigated the \begin{document}${\mathrm{Q}}_2^{{\mathrm{eol}}} $\end{document} loess from the Weibei Loess Tableland in Shaanxi Province as an example to explore the piezoelectricity of loess. …”
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miR-21 Overexpression Promotes Esophageal Squamous Cell Carcinoma Invasion and Migration by Repressing Tropomyosin 1 by Zhuojian Shen, Xia Xu, Liangzhan Lv, Honglue Dai, Ju Chen, Baishen Chen

“…MiR-21 and TPM1 expressions were analyzed by RT-qPCR and WB in 30 ESCC, 10 lung cancer, and 10 bladder cancer clinical specimens, each with matched adjacent normal tissue. …”
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Hantavirus and Leptospira are important causes of nonspecific acute febrile syndrome, Meta, Colombia by Liliana Sánchez-Lerma, Salim Mattar, Verónica Contreras, Jorge Miranda, Vaneza Tique, Virginia Rodríguez, Derly Rodriguez, Sonia Lopez, Andrés Rojas-Gulloso

“…All enrolled patients underwent routine hematology and blood biochemistry examinations. RT-qPCR was performed for Dengue and serology for laboratory diagnoses using ELISA and MAT for Hantavirus and Leptospirosis, respectively. …”
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The Search of miRNA Related to Invasive Growth of Nonfunctioning Gonadotropic Pituitary Tumors by Joanna Boresowicz, Paulina Kober, Natalia Rusetska, Maria Maksymowicz, Agnieszka Paziewska, Michalina Dąbrowska, Natalia Zeber-Lubecka, Jacek Kunicki, Wiesław Bonicki, Jerzy Ostrowski, Janusz A. Siedlecki, Mateusz Bujko

“…The expression of selected miRNAs was assessed in the independent cohort of 80 patients with qRT-PCR. Results. When miRNA profiles of invasive and noninvasive tumors were compared, 29 miRNAs were found differentially expressed. …”
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The e-STROKE Study: The Design of a Prospective Observational Multicentral Study by Kateřina Dvorníková, Veronika Kunešová, Svatopluk Ostrý, Robert Mikulík, Michal Bar

“…The analysis will integrate data from the RES-Q registry and radiological data from the e-STROKE system provided by Brainomix Ltd. …”
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Midgut immune profiling and functional characterization of Aedes aegypti ABC transporter gene(s) using systemic and local bacterial challenges by Vikas Kumar, Shilpi Garg, Diksha Sisodia, Lalita Gupta, Sanjeev Kumar, Vishal Saxena

“…Furthermore, the transcriptional alterations of midgut ABC transporters were explored at different time points upon a thoracic nano-injection (systemic challenge) or infectious blood meal (local challenge) of the bacterial mixture through quantitative real-time PCR (qPCR), and one gene was selected for RNAi-mediated gene silencing and its role assessment in midgut immune responses. …”
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Transcriptomics Analysis of Maize (<i>Zea mays</i> L.) in Response to the Infection by <i>Setosphaeria turcica</i> by Hui Jia, Pan Li, Bu Tao, Yuwei Liu, Zhihang Liu, Mengfang Zhu, He Zhou, Maocun Wang, Jingao Dong, Shouqin Gu, Xiaodong Gong

“…The expression levels of six randomly selected DEGs were validated using qRT-PCR, confirming the accuracy of the RNA-Seq findings. …”
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Comprehensive identification, characterization and expression analyses of the class III POD gene family in water lily (Nymphaea colorata) by Wasi Ullah Khan, Wasi Ullah Khan, Latif Ullah Khan, Noor Muhammad Khan, Ji Zhang, Wang Wenquan, Wang Wenquan, Fei Chen, Fei Chen

“…The analysis of promoter cis-acting elements indicated the presence of regulatory elements associated with various responses such as ABA, MeJA, light responsiveness, anaerobic conditions, and drought inducibility. Finally, the RT-qPCR based expression and enzymes activity of ten NcPOD genes depicted the dynamically differential response to NaCl, heat, cold, and heavy metals (CuSO4 and CdCl2) stresses. …”
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Seroprevalence of anti-ToxoplasmaIgG among the human population in Indonesia: a systematic review and meta-analysis by Taufik Mulya Perdana, Alfin Harjuno Dwiputro, Stefanie Kusuma, Artha Maressa Theodora Simanjuntak, Frengki Prabowo Saputro Wijayanto

“…Heterogeneity was assessed using I2 statistics and Cochran’s Q test. Risk-of-Bias (RoB) was evaluated using the Joanna Briggs Institute Checklist for Prevalence Studies. …”
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Analysis of Mannose‐Binding Lectin Protein and mRNA Levels on Selected Chicken Breeds in South Africa by Peter Ayodeji Idowu, Takalani J. Mpofu, Oliver T. Zishiri, Khathutshelo A. Nephawe, Bohani Mtileni

“…Also 20 liver samples from symptomatic two indigenous chicken breeds, OV (5) and PK (5), and two exotic chicken breeds, RIR (5) and LB (5), were used for MBL expression using quantitative polymerase chain reaction (qPCR) techniques. A general linear model was done using Tukey's multiple comparison post hoc test. …”
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Exoplanet Occurrence Rate with Age for FGK Stars in Kepler by Maryum Sayeed, Ruth Angus, Travis A. Berger, Yuxi(Lucy) Lu, Jessie L. Christiansen, Daniel Foreman-Mackey, Melissa K. Ness

“…We measure the exoplanet occurrence rate as a function of isochrone and gyrochronology ages using confirmed and candidate planets identified in Q1–17 DR25 Kepler data. We employ Kepler's pipeline detection efficiency to correct for the expected number of planets in each age bin. …”
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Starvation Affects the Muscular Morphology, Antioxidant Enzyme Activity, Expression of Lipid Metabolism-Related Genes, and Transcriptomic Profile of Javelin Goby (Synechogobius has... by Xiangning Chen, Yili Xu, Xiangyu Cui, Siying Zhang, Xiangqi Zhong, Juntao Ke, Yuze Wu, Zhiyu Liu, Chaoqing Wei, Zhujin Ding, Jianhe Xu, Hanliang Cheng

“…The enrichment analysis revealed that the DEGs were primarily involved in metabolism-related pathways, including ribosome, TCA pathway, and pyruvate metabolism. Moreover, the qRT-PCR results of 12 DEGs validated the expression trends observed in the RNA-seq data. …”
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Association of Self-DNA Mediated TLR9-Related Gene, DNA Methyltransferase, and Cytokeratin Protein Expression Alterations in HT29-Cells to DNA Fragment Length and Methylation Statu... by István Fűri, Ferenc Sipos, Sándor Spisák, Gergő Kiszner, Barnabás Wichmann, Andrea Schöller, Zsolt Tulassay, Györgyi Műzes, Béla Molnár

“…Expression levels of TLR9-signaling and proinflammatory cytokine-related genes were assayed by qRT-PCR. Methyltransferase activity and cell differentiation were examined by using DNA methyltransferase (DNMT1, -3A, -3B) and cytokeratin (CK) antibodies. …”
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High-Energy Burst-Mode 3.5 μm MIR KTA-OPO by Haowen Guo, Chunyan Jia, Shuai Ye, Yongping Yao, Tiejun Ma, Jiayu Zhang, Meng Bai, Jinbao Xia, Hongkun Nie, Bo Yao, Jingliang He, Baitao Zhang

“…Utilizing a quasi-continuous wave (QCW) laser diode (LD) side-pump module and electro-optic (EO) Q-switching technique, a high beam quality 1064 nm burst-mode laser was achieved as the fundamental source, generating 30 mJ high-energy pulses at burst repetition rates of 100 Hz and 200 Hz with sub-burst repetition rates of 20 kHz, 40 kHz, and 50 kHz. …”
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The members of zinc finger-homeodomain (ZF-HD) transcription factors are associated with abiotic stresses in soybean: insights from genomics and expression analysis by Hafiz Muhammad Rizwan, Jiayi He, Muhammad Nawaz, Keyu Lu, Mingfu Wang

“…Expression profiling through RNA-Seq showed diverse expressions of GmZF-HD genes in different tissues including seeds, roots, shoots and leaves under diverse conditions. Further, the qRT-PCR analysis demonstrated that all tested GmZF-HD genes were significantly induced in soybean leaves, mainly the GmZF-HD5/6/13/39 and GmZF-HD45 genes were significantly upregulated (2.5 to 8.8 folds) under the tested stress treatments compared to control, highlighting their potential roles in response to stresses in soybean. …”
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MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cells by Hou Lingli, Liu Qi, Zhao Ying, Yang Hongwei, Meng Qingying, Yu Fei

“…They consist of the cell proliferation assay, wound healing assay, transwell assay, colony formation assays and qRTPCR. Expression levels of target proteins were determined using Western blotting. …”
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Transcriptome and weighted gene co-expression network analysis of jujube (Ziziphus jujuba Mill.) fruit reveal putative genes involved in proanthocyanin biosynthesis and regulation by Wenqiang Wang, Yunfeng Pu, Hao Wen, Dengyang Lu, Min Yan, Minzhe Liu, Minyu Wu, Hongjin Bai, Lirong Shen, Cuiyun Wu

“…., MYB, bHLH, ERF, bZIP, NAC, SBP, MIKC, HB, WRKY) were considered as hub genes. The results of qRT-PCR analysis validating 10 genes were well consistent with the transcriptome data. …”
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