2′-O-Methylperlatolic Acid Enhances Insulin-Regulated Blood Glucose-Lowering Effect through Insulin Receptor Signaling Pathway by Wang Yinghao, Guan Qiaoli, Liu Guanfu, Wu Xiaoyun, Wang Xuanjun, Sheng Jun

“…Mice were injected with 2-O-M or normal saline and the average blood glucose concentration after 120 min, and the serum levels of insulin, glucagon, and C-peptide were measured. Next, qRT-PCR was performed to detect the mRNA expression of genes involved in lipid and glucose metabolism in the liver and muscle tissues. …”
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Genome-Wide Study of Plant-Specific PLATZ Transcription Factors and Functional Analysis of <i>OsPLATZ1</i> in Regulating Caryopsis Development of Rice (<i>Oryza sativa</i> L.) by Tao Yang, Xin-Tong Xu, Li-Jun Tang, Wen-Tao Wei, Yuan-Yuan Zhao, Jin-Xin Liu, Xue-Feng Yao, Heng Zhao, Chun-Ming Liu, Ai-Ning Bai

“…The expression profile and qRT-PCR results showed that <i>OsPLATZs</i> had distinct expression patterns in different tissues, with some responding to stress induction. …”
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The potential role of differentially expressed tRNA-derived fragments in high glucose-induced podocytes by Zhenxing Zhang, Yunyang Qiao, Jialing Ji, Chan Huang, Huimin Shi, Weihua Gan, Aiqing Zhang

“…The 10 tRFs with the highest differential expression were detected by real-time quantitative polymerase chain reaction (RT-qPCR), and these results were consistent with the sequencing results. …”
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Interactions of the anti-FcRn monoclonal antibody, rozanolixizumab, with Fcγ receptors and functional impact on immune cells in vitro by Omar S. Qureshi, Emma J. Sutton, Rosemary F. Bithell, Shauna M. West, Rona M. Cutler, Gillian McCluskey, Graham Craggs, Asher Maroof, Nicholas M. Barnes, David P. Humphreys, Stephen Rapecki, Bryan J. Smith, Anthony Shock

“…This format is frequently used for therapeutic mAbs due to its intrinsic lower affinity for Fc gamma receptors (FcγR) and lack of C1q engagement. However, with growing evidence suggesting that no Fc-containing agent is truly “silent” in this respect, we explored the engagement of FcγRs and potential functional consequences with rozanolixizumab. …”
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Role and mechanism of dexmedetomidine in regulating bone metabolism in tail-suspended osteoporotic rats by GUO Yunliang, WANG Can, ZHANG Xinyu

“…Secondly, after primary osteoblasts were isolated from the tibiae of tail-suspended rats and then treated with 1 nmol/L Dex, the proportion of alkaline phosphatase (ALP)-positive osteoblasts and the activity of the enzyme were detected by ALP staining and activity test. qRT-PCR was applied to measure the expression of osteogenic activity-related factors, including osteocalcin (Ocn), Runt related transcription factor 2 (Runx2), Osterix protein (Osx), and type 1 collagen (Col1). …”
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The Impact of PTPRK and ROS1 Polymorphisms on the Preeclampsia Risk in Han Chinese Women by Huihui Li, Xingyu Yan, Man Yang, Mei Liu, Shan Tian, Mengru Yu, Wei-Ping Li, Cong Zhang

“…Immunohistochemistry was used for localization. Both qPCR and Western blotting were utilized to investigate the levels of PTPRK in placentas of 20 PE and 20 normal pregnancies. …”
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Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes by Rafael Nepomuceno, Bárbara Scoralick Villela, Sâmia Cruz Tfaile Corbi, Alliny De Souza Bastos, Raquel Alves Dos Santos, Catarina Satie Takahashi, Silvana Regina Perez Orrico, Raquel Mantuaneli Scarel-Caminaga

“…The expression of genes, including IL10, JAK1, STAT3, SOCS3, IP10, ICAM1, IFNA, IFNG, STAT1, and IRF1, was investigated by RT-qPCR. Patients with dyslipidemia demonstrated statistically higher expression of the IL10 and IFNA genes, while IFNG, IP10, IRF1, JAK1, and STAT3 were lower in comparison with nondyslipidemic patients. …”
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Cobalt oxide nanoparticles induce cytotoxicity and excessive ROS mediated mitochondrial dysfunction and p53-independent apoptosis in melanoma cells by Hanan R. H. Mohamed, Basma A. Mohamed, George M. Hakeem, Shahd H. Elnawasani, Maria Nagy, Rawan Essam, Ayman Diab, Gehan Safwat

“…Flow cytometric analysis revealed apoptosis and necrosis induction following Co3O4NP exposure at the IC50 concentration value. Results of qRT-PCR analysis demonstrated remarkable dysregulation of apoptotic and mitochondrial genes, including a significant downregulation of apoptotic p53 and mitochondrial ND3 genes and marked upregulation of the anti-apoptotic gene Bcl2. …”
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Role of B7H4 and Fas in regulation of trophoblast cells and development of pre-eclampsia: a cross-sectional study by Xiujuan Dong, Cuicui Nie, Yanli Yin, Haixia Xu, Yanxia Zhang, Lihua Han, Congcong Xu

“…The expression levels of B7H4 and Fas were analysed using western blotting and real-time quantitative polymerase chain reaction (RT-qPCR). The upstream regulators of B7H4 in trophoblasts were predicted and estimated using a luciferase reporter assay. …”
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Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells by Fariborz Bahrami, Filip Kukulski, Joanna Lecka, Alain Tremblay, Julie Pelletier, Liliana Rockenbach, Jean Sévigny

“…The expression of the ectoenzymes was evaluated by RT-PCR, qPCR, and immunoblotting, and their activity was analyzed by RP-HPLC of the products and by detection of Pi produced from the hydrolysis of ATP, ADP, and AMP. …”
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Angesinenolide B, A Phthalide Dimeric Peroxide, Exerts Anti-Inflammatory Properties by Suppressing MAPK/STATs Signaling Pathways and ROS Production by Zhang L, Liu Y, Wang H, Guo S, Lv J

“…ELISA was employed to investigate the generation of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), while qRT-PCR was utilized to analyze the mRNA expressions of TNF-α, IL-6, inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). …”
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Safety of Drinking Water from Primary Water Sources and Implications for the General Public in Uganda by Keneth Iceland, Kasozi, Sarah, Namubiru, Roland, Kamugisha, Ejike, Daniel Eze, Fred, Ssempijja, Alfred, Omachonu Okpanachi, Hellen, Wambui Kinyi, Jovile Kasande, Atusiimirwe, Dickson Stuart, Tayebwa, Joy, Suubo, Edgar Mario, Fernandez, Nathan, Nshakira, Andrew, Tamale

“…A spatial map was drawn using qGIS®, and analysis of quantitative data was done using MS Excel 2013 at 95% significance. …”
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Designing a financial literacy development model for Farhangian University students by Mohsen Molapanah, Yousef Namvar, Azam Rastgoo, Majid Ahmadlu, Toran Soleimani

“…The population of the quantitative part consisted of all the students of Farhangian universities in Ardabil province, and to determine the sample size, the structural equation modeling sampling formula (q ≤ n ≤ 15q5) was used, where q is the number of questions in the questionnaire and n is the desired sample size (Homan, 2013).. …”
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Integrated microbiome, transcriptome and metabolome insight into the alleviating mechanisms of sheep milk in a DSS-induced colitis mouse model by Wenna Yao, Ruilin Du, Shuo Yan, Teligun Bao, Huimin Zhang, Fang Yang, Yue Xue, Yulong Zhao, Siqin Bao, Xihe Li, Yongli Song

“…ELISA and qPCR analysis showed that relative mRNA expression levels of TNF-α, IL-1β, IL-6, and IL-17 were decreased in SM treatment compared to DSS-induced colitis mice. …”
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Silicon photonic modulators with a 2 × 1 Fabry–Perot cavity by Cao Hengzhen, Xie Jin, Sun Weichao, Zhu Mingyu, Xiang Yuluan, Zhang Gong, Guo Jingshu, Shi Yaocheng, Dai Daoxin

“…In particular, the straight modulation section in the FP cavity is broadened to be far beyond the single-mode regime, alleviating the inherent sensitivity to the variations of waveguide dimensions and thus reducing stochastic resonance-wavelength variations. The Q factor of the FP cavity is manipulated by optimally manipulating the reflection of the AMWGs, and the modulation bandwidth is enhanced to be over 40 GHz by utilizing the optical peaking enhancement effect, which happens when operating at the wavelength slightly detuning to its resonance wavelength. …”
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Enhancing the Therapeutic Effect and Bioavailability of Irradiated Silver Nanoparticle-Capped Chitosan-Coated Rosuvastatin Calcium Nanovesicles for the Treatment of Liver Cancer by Tamer Mohamed Mahmoud, Mohamed Mahmoud Abdelfatah, Mahmoud Mohamed Omar, Omiya Ali Hasan, Saad M. Wali, Mohamed S. El-Mofty, Mohamed G. Ewees, Amel E. Salem, Tarek I. Abd-El-Galil, Dina Mohamed Mahmoud

“…Nop had an entrapment efficiency percent (EE%) of 86.2%, a polydispersity index (PDI) of 0.254, a zeta potential (ZP) of −35.3 mV, and a drug release after 12 h (Q12) of 55.6%. The chitosan-coated optimized formulation (CS.Nop) showed significant mucoadhesive strength that was 1.7-fold greater than Nop. …”
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Informing grassland ecosystem modeling with in-situ and remote sensing observations by Johny Arteaga, Melannie D Hartman, William J Parton, Maosi Chen, Wei Gao

“…The model’s phenology was informed by the MODIS MCD12Q2 product, which showed good agreement with in-situ observations of growing season commencement and duration across different grassland ecosystems, and with observed historical trends. …”
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The <i>Meq</i> Genes of Nigerian Marek’s Disease Virus (MDV) Field Isolates Contain Mutations Common to Both European and US High Virulence Strains by Joseph N. Patria, Luka Jwander, Ifeoma Mbachu, Levi Parcells, Brian Ladman, Jakob Trimpert, Benedikt B. Kaufer, Phaedra Tavlarides-Hontz, Mark S. Parcells

“…Mutations common to field strains that can overcome vaccine protection were identified in the C-terminal proline-rich repeats of the oncoprotein Meq (Marek’s <i>Eco</i>RI-Q-encoded protein). These mutations in <i>meq</i> have been found to be distinct to their region of origin, with high virulence strains obtained in Europe differing from those having evolved in the US. …”
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Limited Effect of Future Land‐Use Changes on Human Heat Stress and Labor Capacity by Steven J. DeHertog, Anton Orlov, Felix Havermann, Suqi Guo, Iris Manola, Julia Pongratz, Quentin Lejeune, Carl‐Friedrich Schleussner, Inga Menke, Florian Humpenöder, Alexander Popp, Peter Lawrence, George C. Hurtt, Louise Chini, Inne Vanderkelen, Edouard L. Davin, Thomas Reerink, Sonia I. Seneviratne, Hans Verbeeck, Wim Thiery

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RfxCas13d-mediated inhibition of Circ1647 alleviates renal fibrosis via PI3K/AKT signaling pathway by Yufang Ni, Hongmin Zhang, Qianwen Xian, Wenjie Qin, Hongwei Su, Li Wang, Jianchun Li

“…Meanwhile, Immunohistochemistry, qRT-PCR and Western blot were conducted to assess the degree of fibrosis. …”
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