A Rare Case of Severe Congenital RYR1-Associated Myopathy by Nicola Laforgia, Manuela Capozza, Lucrezia De Cosmo, Antonio Di Mauro, Maria Elisabetta Baldassarre, Francesca Mercadante, Anna Laura Torella, Vincenzo Nigro, Nicoletta Resta

“…The diagnostic procedure was completed with the complete exome sequencing of the proband and of his parents and his sister, which showed new mutations in the ryanodine receptor gene (RYR1), which maps to chromosome 19q13.2 and encodes the skeletal muscle isoform of a calcium-release channel in the sarcoplasmic reticulum (RyR1). …”
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The microbial mechanism of maize residue decomposition under different temperature and moisture regimes in a Solonchak by Fan Huang, Wan Zhang, Lihua Xue, Bahar Razavi, Kazem Zamanian, Xiaoning Zhao

“…Using the double exponential equation, the decomposition rates of labile and recalcitrant components, the labile component and cumulative CO2 emissions increased faster with increased moisture than with temperature. The Q10 was greater at 15–25 °C than at 25–35 °C, which increased with the increased soil moisture in the leaf but with decreased soil moisture in the stem at 15–25 °C. …”
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Study on hot deformation behavior and microstructure evolution of ultrafine W-0.5 wt%La2O3 alloy wire during processing by Yuan Yao, Jianwei Guo, Shizhong Wei, Jinghong Yang, Zhou Li, Hongan Geng, Liujie Xu

“…Lanthanum oxide gradually aggregates to the grain boundary during the hot deformation process, which improves the thermal deformation activation energy of tungsten. The Q value is about 410.018 kJ/mol, which improves the high temperature strength of tungsten alloy. …”
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Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA by Qingdong Ma, Xiaoxiang Chen, Ke Zhang, Dahong Yao, Lu Yang, Hangyu Wang, Santai Bulemasi, Jian Huang, Jinhui Wang

“…The model is supported by good R and Q coefficients. In addition, different haplotypes of S. involucrata can be discriminated in the OPLS-DA model using the 20 common peaks. …”
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Immunological Aspects of Fulminant Type 1 Diabetes in Chinese by Zhen Wang, Ying Zheng, Yiting Tu, Zhijie Dai, Jian Lin, Zhiguang Zhou

“…The mRNA levels of several related cytokines and molecules, such as IFN-γ, IL-4, RORC, and IL-17 in PBMCs from FT1D patients were analyzed by qRT-PCR. Result. We found that a certain proportion of Chinese FT1D patients actually have developed islet-related autoantibodies after onset of the disease. …”
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واکاوی ارتباط امواج گرمایی با جزایر حرارتی شهر (مطالعه موردی: شهرستان آبادان) by کبری شجاعی زاده, شهریار خالدی, طیبه اکبری ایرانی

“…نقشه کاربری اراضی با 4 کلاس کاربری از فراورده MCD12Q1 سنجنده مودیس به روش طبقه‌بندی نظارت شده استخراج شد. …”
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Lentinan Attenuates Damage of the Small Intestinal Mucosa, Liver, and Lung in Mice with Gut-Origin Sepsis by Zhongshen Kuang, Tingting Jin, ChangYi Wu, Yanan Zong, Panpan Yin, WangYu Dong, Xue Lin, WeiYan You, Chenlong Zhang, Lijie Wang, Yongying Liu, Shan Ren, Jiangwen Yin, Hang Xu

“…In vitro assessment of bacterial translocation was achieved through inoculated culture media. Western blot and RT-qPCR were used to detect the expression of molecules related to the NF-κB signaling pathway in the small intestine tissues of mice. …”
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Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation by Guoyi Dong, Shengpeng Wang, Yuping Ge, Qiuting Deng, Qi Cao, Quanlei Wang, Zhouchun Shang, Wenjie OuYang, Jing Li, Chao Liu, Jie Tang, Weihua Zhao, Ying Gu

“…Interestingly, genes related to inflammatory response, immune response, and angiogenesis were upregulated in spheroids at day 6 and qPCR results further validated the increased expression level of related genes, including STC1, CCL7, HGF, IL24, and TGFB3. …”
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Factors Associated with the Severity of Stress Urinary Incontinence in Elderly Women by Suting Li, Zhi Wang, Lian Yang, Shaoying Liu, Lili Jing, Li Hong

“…Data collection encompassed demographic information, clinical features (including obstetric history, chronic diseases, Urogenital Distress Inventory-6 (UDI-6), and International Consultation on Incontinence Questionnaire-Short Form (ICIQ-SF) scores), as well as physical examinations (including assessments of pelvic floor muscle strength, pelvic organ prolapse quantification (POP-Q) system, and pelvic floor ultrasound imaging). …”
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Effects of Novaluron Exposure on the Oviposition and Expression of Ovarian Development Related Genes in Silkworm, <i>Bombyx mori</i> (Lepidoptera: Bombycidae) by Meng-Jiao Wang, En-Xi Chen, Yi-Lin Ji, Yi-Xuan Qian, Yu-Ming Zhang, Lin Zhu, Guo-Dong Zhao, He-Ying Qian

“…In this study, the effect of trace novaluron on the oviposition of silkworms and histopathological changes were first evaluated, and then the gene expression level changes after novaluron exposure were also determined by employing qRT-PCR. It was found that the number of eggs and the hatching rate of eggs in silkworms decreased significantly after feeding on leaves with a trace amount of novaluron (<i>p</i> ≤ 0.01). …”
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A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice by Guohao Han, Dan Li, Haisen Zhang, Chao Li, Luda Yang, Tiantian Ma, Xuerong Wang, Bairong Ma, Xiaodie Wu, Yang Tao, Ziang Wang, Aihua Wang, Hsu-Wen Chao, Yaping Jin, Huatao Chen

“…Differential expression analysis and RT-qPCR validation confirmed the reliability and validity of the dataset. …”
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Butyric Acid Modulates Gut Microbiota to Alleviate Inflammation and Secondary Bone Loss in Ankylosing Spondylitis by Ke You, Lianjun Yang, Zhihai Su, Jun Shen, Xinyang Fan, Yuanqing Guo, Zhen Yuan, Hai Lu

“…Various techniques, including an enzyme-linked immunosorbent assay (ELISA), magnetic resonance imaging (MRI), micro-CT, histology, quantitative PCR (qPCR) for intestinal tight junction protein expression, and 16S rDNA sequencing to analyze gut microbiota abundance, were employed to assess the inflammation and bone health in the target tissues. …”
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Vegetation Change and Its Response to Climate Change in Yunnan Province, China by Huaizhang Sun, Jiyan Wang, Junnan Xiong, Jinhu Bian, Huaan Jin, Weiming Cheng, Ainong Li

“…In this study, we explored the temporal changes of normalized difference vegetation index (NDVI) in different seasons based on MOD13Q1 NDVI by the maximum value composite and then analyzed spatial distribution characteristics of vegetation using Sen’s tendency estimation, Mann–Kendall significance test, and coefficient of variation model (CV) combined with terrain factors. …”
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Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine by Guanguan Qiu, Ruoyang Zhang, Huifeng Qian, Ruoqiong Huang, Jie Xia, Ruoxi Zang, Zhenkai Le, Qiang Shu, Jianguo Xu, Guoping Zheng, Jiangmei Wang

“…Subsequently, the expression of six miRNAs related to immune responses was examined in a validation cohort of 31 participants via qRT-PCR. Our results validated the differential expression of miR-25-5p, miR-34c-3p, and miR-206 post-booster, with a significant correlation to the receptor binding domain (RBD)-specific antibody. …”
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Curcumin nanoparticles alleviate brain mitochondrial dysfunction and cellular senescence in γ-irradiated rats by Omnia A. Moselhy, Nahed Abdel-Aziz, Azza El-bahkery, Said S. Moselhy, Ehab A. Ibrahim

“…In irradiated rats, CNPs also showed anti-neuroinflammatory effects by reducing brain interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), and nuclear factor-kappa B (NF-ĸB) levels (P < 0.05). Moreover, CNPs administered to irradiated rats significantly reduced brain β-galactosidase activity and the expression levels of p53, p21, and p16 genes (P < 0.05) while concurrently inducing a significant increase in AMPK mRNA expression compared to the irradiated group. …”
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Long non-coding RNA NEAT1 promotes ovarian granulosa cell proliferation and cell cycle progression via the miR-29a-3p/IGF1 axis by Lina He, Jie Lin, Zhengwen Qin, Qing Xu, Li Hao, Yanhong Fu, Xu Ran, Wei Chen

“…The interaction between NEAT1, miR-29a-3p, and IGF1 was examined using dual-luciferase reporter assays, qRT-PCR, and Western blot analysis. Results NEAT1 promoted granulosa cell proliferation and cell cycle progression by indirectly upregulated IGF1 expression through acting as a molecular sponge for miR-29a-3p. …”
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Development and comprehensive greenness assessment of HPLC method for quality control of β-sitosterol in pharmaceutical ointments with trio-color coded evaluation by Haya I. Aljohar, Abdullah M. Al-Hossaini, Seham A. Alzammay, Samiah Alhabardi, Hadir M. Maher, Aya R. Ahmed

“…The developed method was validated for its specificity, linearity, accuracy, precision, the limit of detection, the limit of quantification, robustness, and solution stability based on International Council for Harmonisation (ICH) guidelines Q2 (R1). Our proposed method demonstrated superior performance compared to other reported methods. …”
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The MADS-Box Transcription Factor ClAG2 Is a Key Regulator for the Formation of Double Flower in <i>Clematis</i> L. by Xuerong Chen, Ping Zhou, Nanhong Guo, Yiping Zheng, Xiumei Hou, Lihui Zeng

“…Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed tissue-specific expression patterns among these genes. …”
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The Chloroplastic Small Heat Shock Protein Gene KvHSP26 Is Induced by Various Abiotic Stresses in Kosteletzkya virginica by Xiaohua Liu, Lizi Zhao, Jianzhao Li, Lijun Duan, Kai Zhang, Xuqiang Qiao, Weihuan Li, Chengchao Zheng, Xiaoli Tang, Hongxia Zhang

“…Quantitative real-time PCR (qPCR) assays exhibited that KvHSP26 was constitutively expressed in different tissues such as leaves, stems, and roots, with a relatively higher expression in leaves. …”
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Obesity-induced mesenteric PVAT remodelling is sexually dimorphic, but not driven by ovarian hormones by Lisa Ivatt, Mhairi Paul, Allende Miguelez-Crespo, Patrick W. F. Hadoke, Matthew A. Bailey, Ruth A. Morgan, Mark Nixon

“…Results Transcriptomic analysis of mPVAT revealed sexually dimorphic responses to HFD, with upregulation of extracellular matrix (ECM) remodelling pathways in male but not female mice. Histological and RT-qPCR approaches demonstrated increased collagen deposition and ECM remodelling in mPVAT from obese male compared with obese female mice. …”
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